Abstract:
:We present a new water selective pulse sequence allowing rapid determination of exchange rates of labile protons on the millisecond time scale. Using diffusion measurements, exchange rates of resolved protons can be determined without prior knowledge of relaxation parameters in a short overnight experiment. The use of a sensitive, highly selective and easy to implement water excitation scheme allows for its straightforward application to a wide range of biomolecules. The results obtained for the imino proton exchange rates of a 16 bp DNA are in strong agreement with values obtained by the classical approach of two-dimensional exchange spectroscopy.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Böckmann A,Guittet Edoi
10.1016/s0014-5793(97)01360-4subject
Has Abstractpub_date
1997-11-24 00:00:00pages
127-30issue
1-2eissn
0014-5793issn
1873-3468pii
S0014-5793(97)01360-4journal_volume
418pub_type
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