Abstract:
:Lipoic acid is a coenzyme essential to the activity of enzymes such as pyruvate dehydrogenase, which play important roles in central metabolism. However, neither the enzymes responsible for biosynthesis nor the biosynthetic event of lipoic acid has been reported in mammalian cells. In this study, a mouse mLIP1 cDNA for lipoic acid synthase has been identified. We have shown that the cDNA encodes a lipoic acid synthase by its ability to complement a mutant of Escherichia coli defective in lipoic acid synthase and that mLIP1 is targeted into the mitochondria. These findings suggest that mammalian cells are able to synthesize lipoic acid in mitochondria.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Morikawa T,Yasuno R,Wada Hdoi
10.1016/s0014-5793(01)02469-3keywords:
subject
Has Abstractpub_date
2001-06-01 00:00:00pages
16-21issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(01)02469-3journal_volume
498pub_type
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