Human S100A9 potentiates IL-8 production in response to GM-CSF or fMLP via activation of a different set of transcription factors in neutrophils.

abstract：:Only one copy of the cell adhesion molecule L1 gene is present in the mouse genome, and only one mRNA of 6 kilobases (kb) is expressed in mouse brain [1987, Neurosci. Lett. 82, 89-94]. We have constructed 5 synthetic oligonucleotide probes covering different parts of the published mouse L1 cDNA sequence. Using these p...

journal_title：FEBS letters

authors： Gaardsvoll H,Andersson AM,Bock E

更新日期：1991-12-02 00:00:00

abstract：:3C-like (3CL) protease is essential for the life cycle of severe acute respiratory syndrome-coronavirus (SARS-CoV) and therefore represents a key anti-viral target. A compound library consisting of 960 commercially available drugs and biologically active substances was screened for inhibition of SARS-CoV 3CL protease....

journal_title：FEBS letters

authors： Hsu JT,Kuo CJ,Hsieh HP,Wang YC,Huang KK,Lin CP,Huang PF,Chen X,Liang PH

更新日期：2004-09-10 00:00:00

abstract：:The aim of the present study is to determine the role of intracellular Ca(2+) in VEGF signaling. We demonstrate that reduction in Ca(2+) by chelating compound BAPTA-AM or by IP(3)-endoplasmic reticulum blocker 2-APB selectively inhibited VEGF-induced activation of c-Src-PI3K-Akt but not ERK1/2 in human coronary artery...

journal_title：FEBS letters

authors： Lee M,Spokes KC,Aird WC,Abid MR

更新日期：2010-07-16 00:00:00

abstract：:Syntenin has crucial roles in cell adhesion, cell migration and synaptic transmission. Its closely linked postsynaptic density-95, discs large 1, zonula occludens-1 (PDZ) domains typically interact with C-terminal ligands. We profile syntenin PDZ1-2 through proteomic peptide phage display (ProP-PD) using a library tha...

journal_title：FEBS letters

authors： Garrido-Urbani S,Garg P,Ghossoub R,Arnold R,Lembo F,Sundell GN,Kim PM,Lopez M,Zimmermann P,Sidhu SS,Ivarsson Y

更新日期：2016-01-01 00:00:00

abstract：:Bacteriophage T5 Bg/II/Hind III DNA fragment (803 basepairs), containing the genes for 2 tRNAs and 2 RNAs with unknown functions, was cloned in the plasmid pBR322. The analysis of DNA sequence indicates that tRNA genes code isoacceptor tRNAsSer (tRNA1Ser and tRNA2Ser) with anticodons UGA and GGA, respectively. The mai...

journal_title：FEBS letters

authors： Kryukov VM,Ksenzenko VN,Kaliman AV,Bayev AA

更新日期：1983-07-11 00:00:00

abstract：:Messenger RNA from intact rat pancreatic islets, or from transformed hamster beta (HIT) cells, hybridized with the cDNA probe for type I (but not type II) phospholipase A2. The levels of phospholipase A2 mRNA increased in islets from fasted rats; they decreased in islets cultured in a high glucose concentration (contr...

journal_title：FEBS letters

authors： Metz S,Holmes D,Robertson RP,Leitner W,Draznin B

更新日期：1991-12-16 00:00:00

abstract：:PsbY is one of the low molecular mass subunits of oxygen-evolving photosystem II (PSII). Its location, however, has not been identified in the current crystal structure of PSII. We constructed a PsbY-deletion mutant of Thermosynechococcus elongatus, crystallized, and analyzed the crystal structure of the mutant PSII d...

journal_title：FEBS letters

authors： Kawakami K,Iwai M,Ikeuchi M,Kamiya N,Shen JR

更新日期：2007-10-16 00:00:00

abstract：:Bcl-xL, a close homolog of Bcl2, is an important regulator of apoptosis and is overexpressed in human cancer. Phosphorylation of Bcl-xL can be induced by microtubule-damaging drugs such as taxol or 2-methoxyestradiol (2-ME). By site-directed mutagenesis studies, we have identified that serine 62 is the necessary site ...

journal_title：FEBS letters

authors： Basu A,Haldar S

更新日期：2003-03-13 00:00:00

abstract：:We describe the use of a phage-displayed random pentadecamer peptide library for searching glycosphingolipid mimicking peptides. Two phage clones (AD-1 and AD-2) were selected by biopanning using monoclonal antibody AD117m, directed to lactotetraosylceramide (Lc4Cer). The amino acid sequences of the selected clones sh...

journal_title：FEBS letters

authors： Taki T,Ishikawa D,Hamasaki H,Handa S

更新日期：1997-11-24 00:00:00

abstract：:Spectral variants of the green fluorescent protein (GFP) have been extensively used as reporters to image molecular interactions in living cells by fluorescence resonance energy transfer (FRET). However, those GFP variants which are the most efficient donor acceptor pairs for FRET measurements show a high degree of sp...

journal_title：FEBS letters

authors： Zimmermann T,Rietdorf J,Girod A,Georget V,Pepperkok R

更新日期：2002-11-06 00:00:00

abstract：:Microsomal membranes from the slow-growing Morris hepatoma 9618A catalyze, in the presence of t-butyl hydroperoxide, lower rates of lipid peroxidation than rat liver microsomes. The cytochrome P-450 content of hepatoma microsomes is about 40% that of the liver. SKF 525-A, an inhibitor of mixed-function oxidase, produc...

journal_title：FEBS letters

authors： Borrello S,Galeotti T,Palombini G,Minotti G

更新日期：1986-12-15 00:00:00

abstract：:The dynamic properties of 111 backbone HN sites in uncomplexed human profilin, a protein of 139 residues, have been characterized by two-dimensional inverse-detected 1H-15N NMR spectroscopy. Heteronuclear (1H)-15N nuclear Overhauser effects and 15N longitudinal and transverse relaxation rates have been analyzed in ter...

journal_title：FEBS letters

authors： Constantine KL,Friedrichs MS,Bell AJ,Lavoie TB,Mueller L,Metzler WJ

更新日期：1993-12-28 00:00:00

abstract：:A new approach is suggested for delineating the structural and functional amino acid residues in proteins with known three-dimensional structure, basing on the involvement of residues in intramolecular hydrophobic and hydrophilic interactions and additional information about the conservativity of the residues. The app...

journal_title：FEBS letters

authors： Golovanov AP,Efremov RG,Jaravine VA,Vergoten G,Arseniev AS

更新日期：1995-11-13 00:00:00

abstract：:Signaling via G-protein coupled receptors is initiated by receptor-catalyzed nucleotide exchange on Galpha subunits normally bound to GDP and Gbetagamma. Activated Galpha . GTP then regulates effectors such as adenylyl cyclase. Except for Gbetagamma, no known regulators bind the adenylyl cyclase-stimulatory subunit Ga...

journal_title：FEBS letters

authors： Johnston CA,Ramer JK,Blaesius R,Fredericks Z,Watts VJ,Siderovski DP

更新日期：2005-10-24 00:00:00

abstract：:Azotobacter vinelandii (Av) and chroococcum (Ac) ferredoxin I contain [3Fe-4S]1 + 0 and [4Fe-4S]2+1+ clusters, when isolated aerobically, which undergo one-electron redox cycles at potentials of -460 +/- 10 mV (vs SHE) at pH 8.3 and -645 +/- 10 mV, respectively. The X-ray structure of Fd I (Av) reveals that the N-term...

journal_title：FEBS letters

authors： Thomson AJ

更新日期：1991-07-22 00:00:00

abstract：:Anandamide (AEA) exhibits anti-inflammatory effects. However, its role in the periodontal field remains unknown. Here, we found that gingival crevicular fluid contained a detectable level of AEA. The cannabinoid receptors CB1 and CB2 were expressed by human gingival fibroblasts (HGFs), and markedly upregulated under p...

journal_title：FEBS letters

authors： Nakajima Y,Furuichi Y,Biswas KK,Hashiguchi T,Kawahara K,Yamaji K,Uchimura T,Izumi Y,Maruyama I

更新日期：2006-01-23 00:00:00

abstract：:To search for negative regulatory components of the NF-kappaB activation pathways, we mutagenized Rat-1 fibroblasts and established a stable mutant cell line with a constitutive NF-kappaB activity. This mutant cell line, designated as TK26, showed permanently elevated I kappa B kinase (IKK) activity and a genetically ...

journal_title：FEBS letters

authors： Chinanonwait N,Miura H,Yamamoto N,Yamaoka S

更新日期：2002-11-20 00:00:00

abstract：:The two cytoskeletal proteins VASP and WASP and the protein Homer share a conserved domain, currently designated the WHI domain (WASP homology domain 1) or EVH1 domain (ENA/VASP homology domain 1), which could play an important role in various cellular events such as transport, folding of proteins, and signal transduc...

journal_title：FEBS letters

authors： Callebaut I,Cossart P,Dehoux P

更新日期：1998-12-18 00:00:00

abstract：:tRNAs play a central role in protein translation, acting as the carrier of amino acids. By cloning microRNAs, we unexpectedly obtained some tRNA fragments generated by tRNA cleavage in the anticodon loop. These tRNA fragments are present in many cell lines and different mouse tissues. In addition, various stress condi...

journal_title：FEBS letters

authors： Fu H,Feng J,Liu Q,Sun F,Tie Y,Zhu J,Xing R,Sun Z,Zheng X

更新日期：2009-01-22 00:00:00

abstract：:The involvement of H2O2 generated by photosystem II (PSII) in the process of photoinhibition of thylakoids with a functional oxygen-evolving complex (OEC) was investigated. The rate of photoinhibition was decreased to the rate of loss of activity in the dark when bovine Fe-catalase was present during the photoinhibito...

journal_title：FEBS letters

authors： Bradley RL,Long KM,Frasch WD

更新日期：1991-07-29 00:00:00

abstract：:Apomyoglobin is a widely used model for studying the molecular mechanisms of globular protein folding. This work aimed to analyze the effects of rigidity and length of loops linking protein secondary structure elements on the stability of the molten globule intermediate state. For this purpose, we studied folding/unfo...

journal_title：FEBS letters

authors： Majorina MA,Balobanov VA,Uversky VN,Melnik BS

更新日期：2020-10-01 00:00:00

abstract：:Antipsychotic drugs of known antidiarrhoeal and anticalmodulin activity inhibited the cholera-toxin-catalysed ADP-ribosylation of proteins of Mr 37,000, 40,000 and 45,000 (thought to be regulatory components of the adenylate cyclase complex) that was previously shown to occur in plasma membranes from rabbit intestinal...

journal_title：FEBS letters

authors： Longbottom D,van Heyningen S

更新日期：1990-10-15 00:00:00

abstract：:A 26 kDa peptide has been cleaved from the C-terminus of the S1 heavy chain with formic acid. Cleavage occurs in the '50 kDa' domain probably at the Asp-600-Pro-601 bond. This fragment has been renatured in the presence of the A2 light chain and the 26 kDa(A2) complex shown to interact with actin in an ATP-sensitive m...

journal_title：FEBS letters

authors： Griffiths AJ,Trayer IP

更新日期：1989-01-02 00:00:00

abstract：:Hepatitis C virus (HCV) replication and pathogenesis involve both virus-encoded proteins and cellular factors. In our study, we showed that NS5B, the HCV RNA-dependent RNA polymerase, interacted with M2 type pyruvate kinase (M2PK) but not L type pyruvate kinase. We confirmed the interaction by GST pull down, coimmunop...

journal_title：FEBS letters

authors： Wu X,Zhou Y,Zhang K,Liu Q,Guo D

更新日期：2008-06-25 00:00:00

abstract：:Protein kinase C (PKC) from human promyelocytic leukemia HL-60 cells can be resolved into three fractions (peak, a, b and c) by hydroxyapatite column chromatography. Peak a and c enzymes are indistinguishable from the brain type II PKC having beta (beta I and beta II)-sequence and type III having alpha-sequence, respe...

journal_title：FEBS letters

authors： Hashimoto K,Kishimoto A,Aihara H,Yasuda I,Mikawa K,Nishizuka Y

更新日期：1990-04-09 00:00:00

abstract：:Stimulation of platelets results in the liberation of arachidonic acid (AA) which is further metabolized via the cyclooxygenase or lipoxygenase (LPG) pathway. We have examined the effect of inhibition of LPG on (i) the ADP-induced increase of cytoplasmic Ca2+ concentration and (ii) platelet aggregation. Lipoxygenase i...

journal_title：FEBS letters

authors： Borin ML,Pinelis VG,Ivanova MA,Kudinov YuV,Azizova OA,Markov CM,Khodorov BI

更新日期：1989-11-06 00:00:00

abstract：:Transcription mediators are evolutionarily conserved from yeast to human. We previously reported the specific in vivo roles of mediators during development. Transcriptional mediators including med-6, med-7, and med-10 were shown to be involved in the regulated transcription of specific genes, but not in the transcript...

journal_title：FEBS letters

authors： Kwon JY,Kim-Ha J,Lee BJ,Lee J

更新日期：2001-11-23 00:00:00

abstract：:The use of tobacco plants as a novel expression system for the production of human homotrimeric collagen I is presented in this report. Constructs were engineered from cDNA encoding the human proalpha1(I) chain to generate transgenic tobacco plants expressing collagen I. The recombinant proalpha1(I) chains were expres...

journal_title：FEBS letters

authors： Ruggiero F,Exposito JY,Bournat P,Gruber V,Perret S,Comte J,Olagnier B,Garrone R,Theisen M

更新日期：2000-03-03 00:00:00

abstract：:Spermidine N1-acetyltransferase in an extract from phytohemagglutinin-stimulated bovine lymphocytes was inactivated by preincubation with alkaline phosphatase. Inactivation of the acetylase with the phosphatase was totally inhibited by addition of pyrophosphate. These results suggest that spermidine N1-acetyltransfera...

journal_title：FEBS letters

authors： Matsui I,Otani S,Kamei M,Morisawa S

更新日期：1982-12-13 00:00:00

abstract：:Human CBS1 is a methionine sulfoxide reductase of type B (MSRB) as it specifically reduced Met-R-SO in peptides with dithiothreitol or the thioredoxin system as reductants. Mutation C169S in the active site completely abolished enzymatic activity, while mutation W110A only reduced activity and C105S had no effect. Lik...

journal_title：FEBS letters

authors： Jung S,Hansel A,Kasperczyk H,Hoshi T,Heinemann SH

更新日期：2002-09-11 00:00:00