Abstract:
:Anandamide (AEA) exhibits anti-inflammatory effects. However, its role in the periodontal field remains unknown. Here, we found that gingival crevicular fluid contained a detectable level of AEA. The cannabinoid receptors CB1 and CB2 were expressed by human gingival fibroblasts (HGFs), and markedly upregulated under pathological conditions. AEA significantly reduced the production of pro-inflammatory mediators (IL-6, IL-8 and MCP-1) induced by Porphyromonas gingivalis LPS in HGFs, and this effect was attenuated by AM251 and SR144528, selective antagonists of CB1 and CB2, respectively. Moreover, AEA completely blocked LPS-triggered NF-kappaB activation, implying that AEA may regulate hyperinflammatory reactions in periodontitis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Nakajima Y,Furuichi Y,Biswas KK,Hashiguchi T,Kawahara K,Yamaji K,Uchimura T,Izumi Y,Maruyama Idoi
10.1016/j.febslet.2005.12.079keywords:
subject
Has Abstractpub_date
2006-01-23 00:00:00pages
613-9issue
2eissn
0014-5793issn
1873-3468pii
S0014-5793(05)01564-4journal_volume
580pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::We have investigated the availability of zinc in the periplasmic space of Escherichia coli using a mutant Cu,Zn superoxide dismutase whose dimerization is triggered by zinc binding. This mutant enzyme accumulates in the monomeric form when wild type cells are grown in minimal medium, but assembles in the dimeric form ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.06.008
更新日期:2004-07-02 00:00:00
abstract::The dinuclear paramagnetic center of the soluble CuA domain of the cytochrome c oxidase from Bacillus subtilis has been studied using 1H NMR. The spectrum possesses remarkably sharp shifted resonances. Comparison with the spectrum of the CuA amicyanin variant provides the spin density distribution in the CuA site of c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00981-7
更新日期:1996-10-07 00:00:00
abstract::LUSH is a soluble odorant-binding protein of the fruit fly Drosophila melanogaster. Mutants not expressing this protein have been reported to lack the avoidance behaviour, exhibited by wild type flies, to high concentrations of ethanol. Very recently, the three-dimensional structure of LUSH complexed with short-chain ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(03)01521-7
更新日期:2004-01-30 00:00:00
abstract::The early response gene IEX-1 modulates apoptosis and cell growth in a poorly defined fashion. Here, we describe the effect of hammerhead ribozymes specifically disrupting IEX-1 expression in 293 cells. Compared to vector control, 293 cells exhibit a reduced growth rate and a slowed cell cycle progression, when stably...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02344-4
更新日期:2001-04-13 00:00:00
abstract::Tyrosine O-sulfation is one of the post-translational modification processes that occur to membrane proteins and secreted proteins in eukaryotes. Tyrosylprotein sulfotransferase (TPST) is responsible for this modification, and in this report, we describe the expression pattern and the biological role of TPST-A in the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.11.044
更新日期:2005-01-03 00:00:00
abstract::Reversible phosphorylation of myosin subunits is observed in almost all eukaryotic cells. The data concerning sites and effects of phosphorylation on actin-activated ATPase activity of myosin and on its filament formation are described. These observations are discussed in terms of possible evolutionary trends and rule...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(86)80806-7
更新日期:1986-08-18 00:00:00
abstract::Benzimidazoles are widely used as anthelmintic agents and systemic fungicides. In susceptible organisms, benzimidazoles bind to beta-tubulin and block microtubule polymerization. To further characterize this interaction, site-directed mutagenesis followed by gene replacement was used to change Saccharomyces cerevisiae...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00334-1
更新日期:1996-04-29 00:00:00
abstract::In adipogenesis, growth factors play a crucial role. Using serum depleted condition, we studied the causal role of endothelin-1 (ET-1) and epidermal growth factor (EGF), separately or together, in adipocyte differentiation of 3T3-L1 cells. ET-1 stimulation caused an anti-adipogenic response and this effect was potenti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.09.032
更新日期:2006-10-16 00:00:00
abstract::Circular dichroism (CD) spectra have been obtained from several variants of green fluorescent protein: blue fluorescent protein (BFP), enhanced cyan fluorescent protein (CFP), enhanced green fluorescent protein (GFP), enhanced yellow fluorescent protein (YFP), all from Aequorea victoria, and the red fluorescent protei...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02808-9
更新日期:2002-06-19 00:00:00
abstract::The covalent binding of pyridoxal 5'-phosphate (PLP) to human serum albumin (HSA) is important in the regulation of PLP metabolism. In plasma, PLP is bound to HSA at a single high-affinity and at two or more nonspecific sites. To characterize the primary PLP binding site, HSA was incubated with [3H] PLP, and the Schif...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80073-p
更新日期:1992-02-24 00:00:00
abstract::In human platelet membranes enhanced tyrosine phosphorylation of certain proteins was observed when Zn2+ instead of Mg2+ or Mn2+ was used as a divalent cation for the kinase reaction. An enhanced level of phosphate incorporation into tyrosine residues occurred into a 68 kDa polypeptide besides the 45 kDa and 105 kDa p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81232-8
更新日期:1988-08-01 00:00:00
abstract::PsbV2 is a c-type cytochrome present in a very low abundance in the thermophilic cyanobacterium Thermosynechococcus elongatus. We purified this cytochrome and solved its crystal structure at a resolution of 1.5Å. The protein existed as a dimer in the crystal, and has an overall structure similar to other c-type cytoch...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.08.023
更新日期:2013-10-01 00:00:00
abstract::Cytochrome oxidase o has been isolated from the obligately aerobic, methylotrophic bacterium Methylophilus methylotrophus in the form of a cytochrome cL-o complex. The latter is comprised of cytochrome cL (Mr 21 000) and cytochrome o (Mr 29 000) in a 1-2:1 ratio, possibly in association with one or more minor polypept...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(82)80599-1
更新日期:1983-05-08 00:00:00
abstract::Sequential reverse transcription and polymerase chain reaction (RT-PCR) of the mRNA were used to investigate the expression of dopamine receptors in human peripheral blood lymphocytes. The RT-PCR products contained three types of sequences, each corresponding to those of the D5 dopamine receptor gene and the two relat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81452-r
更新日期:1992-12-07 00:00:00
abstract::A lowered efficiency of oxidative phosphorylation was recently found in a Leber hereditary optic neuropathy (LHON) proband carrying a mutation in the mtDNA gene for subunit 6 of the membrane-bound F0 segment of the F1F0-ATP synthase [9]. This phenotype was transferred to cytoplasmic hybrid cells together with the muta...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00757-6
更新日期:1997-07-28 00:00:00
abstract::Phosphorylation of the basal site with glycogen synthase kinase-4 enhanced the rate of phosphorylation of the regulatory site by cyclic AMP-dependent protein kinase 1.7-fold. In contrast, the phosphorylation state of the regulatory site did not affect the rate of phosphorylation of the basal site with glycogen synthas...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80390-9
更新日期:1988-05-09 00:00:00
abstract::We have examined the formation of DNA triple helices between the oligonucleotides T8XT8 (X = A,C,G,T) and DNA fragments containing the target sequences A8XA8.T8YT8 (X = T,C,G; Y = A,G,C), by DNase I footprinting. We find that A8GA8.T8CT8 yields a footprint with T8CT8 and shows a weaker interaction with T17 and T8GT8. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80510-2
更新日期:1993-10-11 00:00:00
abstract::So far, various approaches for phylogenetic analysis have been developed. Almost all of them put stress on analyzing nucleic acid sequences or protein primary structures. In this paper, we take the physicochemical properties of amino acids into account and introduce the hydropathy profile of amino acids into phylogene...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.08.086
更新日期:2006-10-02 00:00:00
abstract::The mucosal epithelium secretes a variety of antimicrobial peptides that act as part of the innate immune system to protect against invading microbes. Here, we describe the functional properties of human defensin (HD) 5, the major antimicrobial peptide produced by Paneth cells in the ileum, in relation to its structur...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.12.036
更新日期:2007-02-06 00:00:00
abstract::The Bacillus thuringiensis Cry toxin causes swelling and necrosis in insect cells, but the route(s) and significance of the water influx involved in its cytotoxicity are unclear. Here, we assessed the role of aquaporins (AQPs), known as water channels, in Cry toxin intoxication. An AQP inhibitor did not interfere with...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12506
更新日期:2017-01-01 00:00:00
abstract::Using time-resolved low-temperature spectroscopy, we have examined whether or not bovine rhodopsin has a unique transducin-binding state, meta Ib, previously detected from chicken rhodopsin. Unlike chicken meta Ib, bovine meta Ib was detected only by detailed kinetics analysis of the bleaching process, but it was stab...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00216-6
更新日期:1998-03-20 00:00:00
abstract::Polyubiquitylation leading to proteasomal degradation is a well-established mechanism for regulating TGF-β signal transduction components such as receptors and Smads. Recently, an equally important role was suggested for monoubiquitylation of both Smad4 and receptor-associated Smads that regulates their function witho...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2012.03.037
更新日期:2012-07-04 00:00:00
abstract::Three novel neuropeptides, isolated from ganglia of the African giant snail, Achatina fulica, were named WWamide-1, -2 and -3. These substances were biologically active heptapeptide amides with a Trp residue at both the N- and C-termini. WWamide-1, which displayed an inhibitory activity on a central neuron of the snai...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81458-c
更新日期:1993-05-24 00:00:00
abstract::We have studied the endosome-lysosome transfer of internalized epidermal growth factor receptor (EGFR) complexes in a cell-free system from rat liver. Analytical subfractionation of a postmitochondrial supernatant fraction showed that a pulse of internalized [(125)I]EGF was largely associated with a light endosomal fr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01413-1
更新日期:1999-11-12 00:00:00
abstract::Protein synthesis in cell-free systems of rat liver and kidney decreases markedly with age. Examination of activity changes of the different steps revealed for both types of organs that reduced binding of aminoacyl-tRNA to ribosomes and reduced peptidyl transfer might be of major importance for the decrease in overall...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80948-x
更新日期:1983-08-22 00:00:00
abstract::p21-activated protein kinase gamma-PAK phosphorylates prolactin (PRL) in rat pituitary secretory granules on Ser-177 and on the equivalent site, Ser-179, in recombinant human PRL. This is shown by comparison of phosphopeptide maps with the human PRL mutant S179D. gamma-PAK is present in rat and bovine granules as iden...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02444-4
更新日期:2002-03-27 00:00:00
abstract::The nature of the G-proteins present in the pre- and post-synaptic plasma membranes and in the synaptic vesicles of cholinergic nerve terminals purified from the Torpedo electric organ was investigated. In pre- and post-synaptic plasma membranes, Bordetella pertussis toxin, known to catalyze the ADP-ribosylation of th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80190-4
更新日期:1987-09-28 00:00:00
abstract::The O2-dependent formation of side products during the oxidation of veratryl alcohol (VA) by lignin peroxidase has previously been proposed to start with the attack of H2O on the VA radical cation (VA*+). This initial reaction is unlikely since it would also lead to side product formation in the absence of O2, which i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02379-6
更新日期:2001-01-05 00:00:00
abstract::The addition of 5-azacytidine to erythroleukemic cells which were induced to differentiate with DMSO or BA altered the expression of the hemoglobins. After the addition of 5-azacytidine there was an increase in hemoglobin synthesis especially in the embryonic E2 band. The beta-globin increased in synthesis after 5-aza...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80803-5
更新日期:1983-10-17 00:00:00
abstract::Adenosine can be metabolized by chicken erythrocytes as a carbon source maintaining ATP levels. In addition, it stimulates glucose uptake and lactate production. Adenosine effects do not involve binding to membrane receptors. They are dependent on the provision of a carbon source to glycolysis and correlate with the i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81635-7
更新日期:1989-11-20 00:00:00