Triple helix assembly and processing of human collagen produced in transgenic tobacco plants.

abstract：:A large number of protein-protein interactions involved turn or loop regions. The excised linear peptides from these regions reveal complex conformational averaging. To circumvent this motional averaging and to stabilize the beta-turn conformation, extensive effort has been devoted to the design of constrained peptido...

journal_title：FEBS letters

authors： Lee MS,Gardner B,Kahn M,Nakanishi H

更新日期：1995-02-13 00:00:00

abstract：:Several analogues of heat-stable enterotoxins (STh and STp) produced by enterotoxigenic Escherichia coli were synthesized. Peptides (STh[6-18] and STp[5-17]) consisting of 13 amino acid residues from the Cys residue near the N-terminus to the Cys residue near the C-terminus and linked by three disulfide bonds had the ...

journal_title：FEBS letters

authors： Yoshimura S,Ikemura H,Watanabe H,Aimoto S,Shimonishi Y,Hara S,Takeda T,Miwatani T,Takeda Y

更新日期：1985-02-11 00:00:00

abstract：:The human gut microbiota comprises approximately 100 trillion microbial cells and has a significant effect on many aspects of human physiology including metabolism, nutrient absorption and immune function. Disruption of this population has been implicated in many conditions and diseases, including examples such as obe...

journal_title：FEBS letters

authors： Walsh CJ,Guinane CM,O'Toole PW,Cotter PD

更新日期：2014-11-17 00:00:00

abstract：:Cloning and nucleotide sequence analysis of the human calcitonin mRNA from the BEN lung carcinoma cell line, a cell line known to secrete high-Mr forms of calcitonin, showed no difference in the coding region at the nucleotide level compared with calcitonin mRNA isolated from medullary thyroid carcinoma which secretes...

journal_title：FEBS letters

authors： Riley JH,Edbrooke MR,Craig RK

更新日期：1986-03-17 00:00:00

abstract：:The FMN-heme interdomain (intraprotein) electron transfer (IET) kinetics in full length and oxygenase/FMN (oxyFMN) construct of human iNOS were determined by laser flash photolysis over the temperature range from 283 to 304K. An appreciable increase in the rate constant value was observed with an increase in the tempe...

journal_title：FEBS letters

authors： Li W,Chen L,Fan W,Feng C

更新日期：2012-01-20 00:00:00

abstract：:The efflux of [3H]purines from cultured sympathetic neurons prelabelled with [3H]adenine is accelerated 2-3-fold within hours of nerve growth factor (NGF) withdrawal and is reduced by readdition of NGF. Addition of 8-(4-chlorphenyl-thio) cAMP, which delays neurite degeneration, reduced the enhanced efflux of purines, ...

journal_title：FEBS letters

authors： Tolkovsky AM,Buckmaster EA

更新日期：1989-09-25 00:00:00

abstract：:In lower and higher eukaryotes, a family of tightly related proteins designated eRF1 (for eukaryotic release factor 1) catalyses termination of protein synthesis at all three stop codons. The human genome contains four eRF1 homologous sequences localised on chromosomes 5, 6, 7 and X. We report here the cloning and the...

journal_title：FEBS letters

authors： Guenet L,Toutain B,Guilleret I,Chauvel B,Deaven LL,Longmire JL,Le Gall JY,David V,Le Treut A

更新日期：1999-07-02 00:00:00

abstract：:S100, a subfamily of the EF-hand type calcium sensing proteins, is implicated in many cellular functions including muscle contractility. Two isoforms, S100A1 and S100B, at 2-10 microM significantly inhibit active tension, stiffness and ATPase of skinned single rabbit psoas muscle fibers at sub-maximal (pCa approximate...

journal_title：FEBS letters

authors： Adhikari BB,Wang K

更新日期：2001-05-25 00:00:00

abstract：:N-Oligosaccharyltransferase catalyzes the N-glycosylation of asparagine residues of nascent polypeptide chains in the endoplasmic reticulum, a pathway highly conserved in all eukaryotes. An enzymatically active complex was isolated from microsomal membranes from Saccharomyces cerevisiae, which is composed of four prot...

journal_title：FEBS letters

authors： Knauer R,Lehle L

更新日期：1994-05-09 00:00:00

abstract：:A cell-free protein expression system was established that provides protein samples of adequate concentration and purity for direct NMR analysis. The Escherichia coli peptidyl-prolyl cis-trans isomerase PpiB was expressed in this system with dual amino acid-selective isotope labeling to identify the NMR signal from th...

journal_title：FEBS letters

authors： Guignard L,Ozawa K,Pursglove SE,Otting G,Dixon NE

更新日期：2002-07-31 00:00:00

abstract：:The sequence of the HIV Nef protein has no significant homology to other proteins in the SwissProt database, and experimental data concerning its function are sparse and contradictory. Using a novel protein sequence comparison method, we find similarities between different Nef sequences and the alpha chain of human MH...

journal_title：FEBS letters

authors： Hobohm U,Sander C

更新日期：1993-11-01 00:00:00

abstract：:This work describes the molecular cloning of a variant isoform of the low-affinity levocabastine-sensitive neurotensin receptor isolated from mouse brain. Although the corresponding mRNA encodes for a 282 amino acid protein unable to bind neurotensin after transient transfection in COS-7 cells, this non-functional neu...

journal_title：FEBS letters

authors： Botto JM,Sarret P,Vincent JP,Mazella J

更新日期：1997-01-03 00:00:00

abstract：:Statistically significant similarity was revealed between amino acid sequences of NTP-binding pattern-containing domains which are among the most conserved protein segments in dissimilar groups of ss and dsDNA viruses (papova-, parvo-, geminiviruses and P4 bacteriophage), and RNA viruses (picorna-, como- and nepovirus...

journal_title：FEBS letters

authors： Gorbalenya AE,Koonin EV,Wolf YI

更新日期：1990-03-12 00:00:00

abstract：:The 77 kDa subunit of the polyadenylation cleavage stimulation factor (CstF77) is important in messenger RNA 3' end processing. Previously, we demonstrated that AtCstF77 interacts with AtCPSF30, the Arabidopsis ortholog of the 30 kDa subunit of the Cleavage and Polyadenylation Specificity Factor. In further dissecting...

journal_title：FEBS letters

authors： Bell SA,Hunt AG

更新日期：2010-04-16 00:00:00

abstract：:We propose a unified framework for the identification of functional interaction structures of biomolecular networks in a way that leads to a new experimental design procedure. In developing our approach, we have built upon previous work. Thus we begin by pointing out some of the restrictions associated with existing s...

journal_title：FEBS letters

authors： Cho KH,Choo SM,Wellstead P,Wolkenhauer O

更新日期：2005-08-15 00:00:00

abstract：:It is increasingly clear that microRNAs play a crucial role in tumorigenesis. Recently, emerging evidence suggested that miR-26a is aberrantly expressed in tumor tissues. In our study, frequent down-regulation of miR-26a was observed in 10 human bladder cancer tissues. Forced expression of miR-26a in the bladder cance...

journal_title：FEBS letters

authors： Lin Y,Chen H,Hu Z,Mao Y,Xu X,Zhu Y,Xu X,Wu J,Li S,Mao Q,Zheng X,Xie L

更新日期：2013-08-02 00:00:00

abstract：:The thermal blockage of QA-. oxidation was analysed in PS II membrane fragments by monitoring flash-induced changes of the relative fluorescence quantum yield as a function of temperature. The results obtained reveal: (a) in dark-adapted samples the fraction of QA-. that is not reoxidised within a time domain of 10 s ...

journal_title：FEBS letters

authors： Reifarth F,Renger G

更新日期：1998-05-29 00:00:00

abstract：:Human monoclonal antibodies often display limited thermodynamic and colloidal stabilities. This behavior hinders their production, and places limitations on the development of novel formulation conditions and therapeutic applications. Antibodies are highly diverse molecules, with much of the sequence variation observe...

journal_title：FEBS letters

authors： Rouet R,Lowe D,Christ D

更新日期：2014-01-21 00:00:00

abstract：:Human legumain was characterized following overexpression in a murine cell line as the C-terminal Ig-fusion protein. Upon acid treatment, the prolegumain autoproteolyzed distal to two aspartic acid residues to yield a highly active form. The ability of mature legumain to cleave after aspartic acid residues was confirm...

journal_title：FEBS letters

authors： Halfon S,Patel S,Vega F,Zurawski S,Zurawski G

更新日期：1998-10-30 00:00:00

abstract：:Glycosaminoglycans are known to be associated with extracellular amyloid deposits of various amyloidogenic proteins. In this study, we found that the glycosaminoglycan heparin greatly accelerates the elongation step in fibril formation by the N-terminal 1-83 fragment of human apolipoprotein A-I (apoA-I), especially in...

journal_title：FEBS letters

authors： Mikawa S,Mizuguchi C,Nishitsuji K,Baba T,Shigenaga A,Shimanouchi T,Sakashita N,Otaka A,Akaji K,Saito H

更新日期：2016-10-01 00:00:00

abstract：:All ligands of the epidermal growth factor receptor (EGFR), which has important roles in development and disease, are made as transmembrane precursors. Proteolytic processing by ADAMs (a disintegrin and metalloprotease) regulates the bioavailability of several EGFR-ligands, yet little is known about the enzyme respons...

journal_title：FEBS letters

authors： Sahin U,Blobel CP

更新日期：2007-01-09 00:00:00

abstract：:We show that inverted membrane vesicles from Corynebacterium glutamicum, a Gram-positive bacterium, are able to generate and maintain an electrochemical gradient of protons in response to the addition of NADH. This result indicates that the respiratory chain is intact and that the vesicles are reasonably impermeable t...

journal_title：FEBS letters

authors： Schrempp S,Bayan N,Shechter E

更新日期：1994-12-12 00:00:00

abstract：:We report on the cDNA cloning and characterization of a novel short-chain isoprenyl diphosphate synthase from the aphid Myzus persicae. Of the three IPPS cDNAs we cloned, two yielded prenyltransferase activity following expression in Escherichia coli; these cDNAs encode identical proteins except for the presence, in o...

journal_title：FEBS letters

authors： Vandermoten S,Charloteaux B,Santini S,Sen SE,Béliveau C,Vandenbol M,Francis F,Brasseur R,Cusson M,Haubruge E

更新日期：2008-06-11 00:00:00

abstract：:We provide first large scale analysis of the peculiarities of surface areas of 5658 dissimilar (below 50% sequence similarity) proteins with known 3D-structures that bind to proteins, DNA or RNAs. We show here that area of the protein surface is highly correlated with the protein length. The size of the interface surf...

journal_title：FEBS letters

authors： Wu Z,Hu G,Yang J,Peng Z,Uversky VN,Kurgan L

更新日期：2015-09-14 00:00:00

abstract：:Yeast genes involved in thiamin pyrophosphate (TPP) synthesis are transcriptionally induced in response to thiamin starvation. In this system, three proteins (Thi2p, Thi3p, and Pdc2p) act as positive regulatory factors. Thi3p is a TPP-binding protein and upregulates THI genes expression when TPP is not bound. We found...

journal_title：FEBS letters

authors： Nosaka K,Onozuka M,Konno H,Akaji K

更新日期：2008-12-10 00:00:00

abstract：:T cells express two different types of voltage-independent Ca(2+)-activated K(+) channels with small (SK) and intermediate (IK) conductance that serve important roles in the activation of T lymphocytes. In contrast to the IK channels from T lymphocytes which are upregulated upon mitogen stimulation, SK channels of Jur...

journal_title：FEBS letters

authors： Jäger H,Adelman JP,Grissmer S

更新日期：2000-03-10 00:00:00

abstract：:PsbY is one of the low molecular mass subunits of oxygen-evolving photosystem II (PSII). Its location, however, has not been identified in the current crystal structure of PSII. We constructed a PsbY-deletion mutant of Thermosynechococcus elongatus, crystallized, and analyzed the crystal structure of the mutant PSII d...

journal_title：FEBS letters

authors： Kawakami K,Iwai M,Ikeuchi M,Kamiya N,Shen JR

更新日期：2007-10-16 00:00:00

abstract：:Five-lipoxygenase-activating protein (FLAP) is usually described as an essential protein to activate the leukotriene (LTs) synthesis via the 5-lipoxygenase pathway. In the enterocyte model HT29 cl.19A cell line, 5-lipoxygenase metabolism was found despite the lack of FLAP expression. Therefore HT29 cl.19A represents a...

journal_title：FEBS letters

authors： Battu S,Beneytout JL,Pairet M,Rigaud M

更新日期：1998-10-16 00:00:00

abstract：:Using reverse transcription followed by PCR amplification (RT-PCR), we have identified multiple messenger RNAs encoding for the neuronal pore-forming Ca(2+) channel subunits alpha(1A) (P/Q channel), alpha(1B) (N channel), alpha(1D) (neuronal/endocrine L channel), alpha(1E) (R channel), alpha(1G-H) (T channel) and alph...

journal_title：FEBS letters

authors： García-Palomero E,Cuchillo-Ibáñez I,García AG,Renart J,Albillos A,Montiel C

更新日期：2000-09-22 00:00:00

abstract：:We have investigated the availability of zinc in the periplasmic space of Escherichia coli using a mutant Cu,Zn superoxide dismutase whose dimerization is triggered by zinc binding. This mutant enzyme accumulates in the monomeric form when wild type cells are grown in minimal medium, but assembles in the dimeric form ...

journal_title：FEBS letters

authors： Berducci G,Mazzetti AP,Rotilio G,Battistoni A

更新日期：2004-07-02 00:00:00