Abstract:
:The efflux of [3H]purines from cultured sympathetic neurons prelabelled with [3H]adenine is accelerated 2-3-fold within hours of nerve growth factor (NGF) withdrawal and is reduced by readdition of NGF. Addition of 8-(4-chlorphenyl-thio) cAMP, which delays neurite degeneration, reduced the enhanced efflux of purines, as did the addition of cycloheximide, MgCl2 and the protease inhibitor tosyl-L-lysine chloromethyl ketone. Colchicine accelerated purine efflux and neurite degeneration but 2-deoxyglucose increased purine efflux without inducing degeneration, suggesting that ATP reduction itself is not the cause of neurite degeneration. The increase in purine efflux is thus an early biochemical event that has diagnostic value for the study of NGF action since deprivation is detected well before irreversible changes become established.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tolkovsky AM,Buckmaster EAdoi
10.1016/0014-5793(89)81113-5subject
Has Abstractpub_date
1989-09-25 00:00:00pages
315-20issue
2eissn
0014-5793issn
1873-3468journal_volume
255pub_type
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