Abstract:
:Dark-grown angiosperm seedlings lack chlorophylls, but accumulate protochlorophyllide a complexed with the light-dependent enzyme NADPH:protochlorophyllide oxidoreductase. Previous investigators correlated spectral heterogeneity of in vivo protochlorophyllide forms and a shift of chlorophyllide forms from 680 to 672 nm (Shibata shift) occurring after irradiation, with intact membrane structures which are destroyed by solubilization. We demonstrate here that the various protochlorophyllide forms and the Shibata shift which disappear upon solubilization are restored if the reconstituted complex is treated with plastid lipids and 80% (w/v) glycerol. We hypothesize that the lipids can form a cubic phase and that this is the precondition in vitro and in vivo for the observed spectral properties before and after irradiation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Klement H,Oster U,Rüdiger Wdoi
10.1016/s0014-5793(00)01887-1keywords:
subject
Has Abstractpub_date
2000-09-01 00:00:00pages
306-10issue
2-3eissn
0014-5793issn
1873-3468pii
S0014-5793(00)01887-1journal_volume
480pub_type
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