Abstract:
:Phospholipase A2 (PLA2) from human pancreas, designated hPLA2-I, functions as a digestive enzyme. Interestingly, the present study demonstrated that the mature form of hPLA2-I stimulated the growth of a human pancreatic cancer cell line MIAPaCa-2, whereas the pro-form was ineffective. PLA2s from Laticauda semifasciata fraction I, Crotalus adamanteus venom, Streptomyces violaceoruber and bee venom, showed no proliferative effect to the growth of MIAPaCa-2. The Scatchard plot analysis revealed that the MIAPaCa-2 cell had a specific binding site for the mature hPLA2-I. The equilibrium binding constant (Kd) and the maximum binding capacity (Bmax) were 2.6 nM and 0.4 fmol/10(6) cells, respectively. These results suggest that the mature hPLA2-I, but not the pro-form, may function as a growth factor of pancreas carcinoma via the specific binding site.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hanada K,Kinoshita E,Itoh M,Hirata M,Kajiyama G,Sugiyama Mdoi
10.1016/0014-5793(95)01005-ysubject
Has Abstractpub_date
1995-10-02 00:00:00pages
85-7issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(95)01005-Yjournal_volume
373pub_type
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