Abstract:
:A cDNA sequence putatively encoding a Delta(6)-fatty acid desaturase was isolated from Rhizopus arrhizus using reverse transcription polymerase chain reaction and rapid amplification of cDNA ends methods. Sequence analysis indicated that this cDNA sequence had an open reading frame of 1377 bp encoding 458 amino acids of 52 kDa. The deduced amino acid sequence showed high similarity to those of fungal Delta(6)-fatty acid desaturases which comprised the characteristics of membrane-bound desaturases, including three conserved histidine-rich motifs and hydropathy profile. A cytochrome b(5)-like domain was observed at the N-terminus. To elucidate the function of this novel putative desaturase, the coding sequence was expressed heterologously in Saccharomyces cerevisiae strain INVScl. The result demonstrated that the coding product of the sequence exhibited Delta(6)-fatty acid desaturase activity by the accumulation of gamma-linolenic acid.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Zhang Q,Li M,Ma H,Sun Y,Xing Ldoi
10.1016/s0014-5793(03)01380-2keywords:
subject
Has Abstractpub_date
2004-01-02 00:00:00pages
81-5issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579303013802journal_volume
556pub_type
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