Mitogen-stimulated events in nuclei of Swiss 3T3 cells. Evidence for a direct link between changes of inositol lipids, protein kinase C requirement and the onset of DNA synthesis.

abstract：:In Alzheimer's disease (AD) brain, microtubule-associated protein tau is abnormally modified by hyperphosphorylation and glycosylation, and is aggregated as neurofibrillary tangles of paired helical filaments. To investigate the role of tau glycosylation in neurofibrillary pathology, we isolated various pools of tau p...

journal_title：FEBS letters

authors： Liu F,Zaidi T,Iqbal K,Grundke-Iqbal I,Merkle RK,Gong CX

更新日期：2002-02-13 00:00:00

abstract：:Platelet aggregation is accompanied by the tyrosine phosphorylation of several proteins including syk. However, some of these proteins are not identified. Recent studies showed that LAT is a syk substrate and is tyrosine phosphorylated during T cell stimulation. In this study, we demonstrated that LAT is present in pl...

journal_title：FEBS letters

authors： Sarkar S

更新日期：1998-12-28 00:00:00

abstract：:The amyloid precursor protein (APP) is proteolytically processed by β- and γ-secretases to release amyloid-β peptide (Aβ), the main component found in senile plaques of Alzheimer's disease (AD) patient brains. Alternatively, APP can be cleaved within the Aβ sequence by α-secretase, thus precluding the generation of Aβ...

journal_title：FEBS letters

authors： León-Otegui M,Gómez-Villafuertes R,Díaz-Hernández JI,Díaz-Hernández M,Miras-Portugal MT,Gualix J

更新日期：2011-07-21 00:00:00

abstract：:In a wild-type strain of Saccharomyces cerevisiae, cadmium induces the activities of both gamma-glutamyl transferase (gamma-GT) and glutathione transferase 2 (Gtt2). However, Gtt2 activity did not increase under gamma-GT or Ycf1 deficiencies, suggesting that the accumulation of glutathione-cadmium in the cytosol inhib...

journal_title：FEBS letters

authors： Adamis PD,Mannarino SC,Eleutherio EC

更新日期：2009-05-06 00:00:00

abstract：:The eukaryotic Ssu72 factor is involved in several RNA biogenesis processes. It has phosphatase activity on the carboxy-terminal domain (CTD) of the major subunit of RNA polymerase II. The Kluyveromyces lactis Ssu72 (KlSsu72) shows in vitro phosphatase activity for the pNPP substrate, and this activity is inhibited by...

journal_title：FEBS letters

authors： Rodríguez-Torres AM,Lamas-Maceiras M,García-Díaz R,Freire-Picos MA

更新日期：2013-08-19 00:00:00

abstract：:A chemokine receptor, CXCR4, and its endogenous ligand, stromal cell-derived factor-1 (SDF-1), have been recognized to be involved in the metastasis of several types of cancers. T140 analogs are peptidic CXCR4 antagonists composed of 14 amino acid residues that were previously developed as anti-HIV agents having inhib...

journal_title：FEBS letters

authors： Tamamura H,Hori A,Kanzaki N,Hiramatsu K,Mizumoto M,Nakashima H,Yamamoto N,Otaka A,Fujii N

更新日期：2003-08-28 00:00:00

abstract：:Expression of calcyclin in different cell lines and mouse tissues was determined with polyclonal antibodies raised against calcyclin from Ehrlich ascites tumour (EAT) cells. The protein was detected in mouse skeletal and cardiac muscle, in lung, kidney and spleen, and was especially enriched in mouse smooth muscle as ...

journal_title：FEBS letters

authors： Kuźnicki J,Filipek A,Heimann P,Kaczmarek L,Kamińska B

更新日期：1989-08-28 00:00:00

abstract：:Under hypobaric hypoxia, antioxidant defenses of the heart are stressed by the enhanced production of ROS. Mammalian heart acclimatizes to hypoxia through altered gene expression, which we studied in murine heart exposed to 10h of acute hypobaric hypoxia (AHH), equivalent to 15000ft, using cDNA arrays. Functional clas...

journal_title：FEBS letters

authors： Karar J,Dolt KS,Mishra MK,Arif E,Javed S,Pasha MA

更新日期：2007-10-02 00:00:00

abstract：:In mouse mammary epithelial cells in primary culture, mechanical stimulation of a cell induced in other cells within the same colony a short depolarization of less than 15 mV with a duration of 1-8 s and a subsequent, prominent hyperpolarization of 6 mV lasting 10-40 s. Epidermal growth factor induces a spontaneous hy...

journal_title：FEBS letters

authors： Enomoto K,Furuya K,Maeno T,Edwards C,Oka T

更新日期：1987-10-19 00:00:00

abstract：:Protein import into isolated tobacco mitochondria was investigated using mitochondria from leaves harvested at different times of the day and night. Efficient import was only detected with mitochondria isolated from leaves harvested during the dark period of the growth cycle, only low levels of import were detected fr...

journal_title：FEBS letters

authors： Dessi P,Whelan J

更新日期：1997-09-29 00:00:00

abstract：:In the presence of ADP, the molecular chaperones GroEL and GroES from Escherichia coli not only facilitated the refolding of various proteins, but also prevented their irreversible heat inactivation in vitro. Without nucleotides the refolding reactions were arrested by GroEL. Addition of GroES and ADP to the reaction ...

journal_title：FEBS letters

authors： Kawata Y,Nosaka K,Hongo K,Mizobata T,Nagai J

更新日期：1994-05-30 00:00:00

abstract：:Crosslinking of proteins by tissue transglutaminase (tTG) is enhanced in amyloid (Abeta) deposits characteristic of Alzheimer's disease and sporadic inclusion body myositis. Small heat shock proteins (sHsps) also occur in amyloid deposits. We here report the substrate characteristics for tTG of six sHsps. Hsp27, Hsp20...

journal_title：FEBS letters

authors： Boros S,Kamps B,Wunderink L,de Bruijn W,de Jong WW,Boelens WC

更新日期：2004-10-08 00:00:00

abstract：:Metabolic activities of orexin A (OXA) in mature adipocytes are mediated via PI3K/PKB and PPARγ. However, the effects of OXA on preadipocytes are largely unknown. We report here that OXA stimulates the proliferation and viability of 3T3-L1 preadipocytes and protects them from apoptosis via ERK1/2, but not through PKB....

journal_title：FEBS letters

authors： Skrzypski M,Kaczmarek P,Le TT,Wojciechowicz T,Pruszyńska-Oszmalek E,Szczepankiewicz D,Sassek M,Arafat A,Wiedenmann B,Nowak KW,Strowski MZ

更新日期：2012-11-30 00:00:00

abstract：:The nuclear factor NF-IL6 had been suggested to be responsible for the IL-6-mediated induction of several acute-phase proteins. To obtain evidence for the involvement of NF-IL6 in the induction of acute-phase proteins, we introduced the NF-IL6 gene and its truncated mutant (delNFIL6) gene into a hepatoma cell line Hep...

journal_title：FEBS letters

authors： Natsuka S,Isshiki H,Akira S,Kishimoto T

更新日期：1991-10-07 00:00:00

abstract：:The high affinity (low Km) cyclic GMP phosphodiesterase (PDE) is activated by GTP, while the cyclic AMP PDE is not. GTP and its hydrolysis-resistant analogue, guanylylimidodiphosphate (GppNHp), display a half-maximal stimulating effect at almost the same concentration (5 X 10(-6) M). The GTP stimulating effect is not ...

journal_title：FEBS letters

authors： Spina A,Chiosi E,Paolisso G,Laurenza A,Illiano G

更新日期：1983-07-04 00:00:00

abstract：:We have investigated whether the expression in Arabidopsis thaliana seeds of a transgene (the Phaseolus vulgaris arcelin (arc)5-I gene) could be enhanced by the simultaneous introduction of an antisense gene for an endogenous seed storage protein (2S albumin). Seeds of plants transformed with both the arc5-I gene and ...

journal_title：FEBS letters

authors： Goossens A,Van Montagu M,Angenon G

更新日期：1999-07-30 00:00:00

abstract：:We report that sodium butyrate, a natural product of fiber degradation by colonic bacteria, markedly suppresses c-Myc-mediated apoptosis induced in murine plasmacytomas and human Burkitt lymphomas by growth factor deprivation, but not in cell lines devoid of c-myc translocations. Attenuation of cell death is associate...

journal_title：FEBS letters

authors： Alexandrov I,Romanova L,Mushinski F,Nordan R

更新日期：1998-08-28 00:00:00

abstract：:The vertebrate transcription factor CTCF is not only involved in transcriptional activation, insulation and genomic imprinting, but also in transcriptional repression. Sequence motifs mediating these activities have not been identified so far. We have mapped a short repression motif to residues 150-170 within the N-te...

journal_title：FEBS letters

authors： Drueppel L,Pfleiderer K,Schmidt A,Hillen W,Berens C

更新日期：2004-08-13 00:00:00

abstract：:Combining mutation and chemical modification, we have introduced Asp, Gln, Cys, S-carboxymethylcysteine (Cax) and S-carbamoylmethylcysteine (Cam) into the positions of Glu190 and Glu201 of the beta subunit of F1-ATPase from the thermophilic Bacillus PS3. The steady-state ATPase activities of alpha 3 beta 3 gamma compl...

journal_title：FEBS letters

authors： Amano T,Tozawa K,Yoshida M,Murakami H

更新日期：1994-07-04 00:00:00

abstract：:Macrophage cyclooxygenase-2 (COX-2) transcription is mediated through the collaboration of different promoter elements. Here, the role of an overlapping cyclic AMP responsive element (CRE)/E-box was investigated. Nuclear proteins bound both the CRE and E-box, which synergized with other promoter elements to induce COX...

journal_title：FEBS letters

authors： Mestre JR,Rivadeneira DE,Mackrell PJ,Duff M,Stapleton PP,Mack-Strong V,Maddali S,Smyth GP,Tanabe T,Daly JM

更新日期：2001-05-11 00:00:00

abstract：:A steady-state framework was applied to the ubiquitous tricyclic enzyme cascade structure, as seen in the mitogen-activated protein (MAP) kinase system, to analyze the effect of upstream kinase concentrations on final output response. The results suggest that signal amplification achieved by the cascade structure ensu...

journal_title：FEBS letters

authors： Mutalik VK,Singh AP,Edwards JS,Venkatesh KV

更新日期：2004-01-30 00:00:00

abstract：:Reg, first isolated from a rat regenerating islet cDNA library, is expressed in regenerating islet beta-cells. Recently, it has been revealed that Reg and Reg-related genes constitute a multigene family, the Reg family. In human, the four REG family genes, i.e., REG 1 alpha, REG 1 beta, REG-related sequence (RS) and H...

journal_title：FEBS letters

authors： Miyashita H,Nakagawara K,Mori M,Narushima Y,Noguchi N,Moriizumi S,Takasawa S,Yonekura H,Takeuchi T,Okamoto H

更新日期：1995-12-27 00:00:00

abstract：:We have demonstrated over a decade ago that hepoxilins cause the release of insulin from isolated pancreatic islets of Langerhans in vitro. However, no studies are available so far to indicate whether these compounds are active in vivo. The present study is the first to our knowledge which demonstrates that hepoxilins...

journal_title：FEBS letters

authors： Pace-Asciak CR,Demin PM,Estrada M,Liu G

更新日期：1999-11-19 00:00:00

abstract：:Niemann-Pick C (NPC) disease is a progressive neurological disorder in which cholesterol, gangliosides and bis-monoacylglycerol phosphate accumulate in late endosomes/lysosomes. This disease is caused by mutations in either the NPC1 or NPC2 gene. NPC1 and NPC2 are involved in egress of lipids, particularly cholesterol...

journal_title：FEBS letters

authors： Vance JE

更新日期：2006-10-09 00:00:00

abstract：:A novel gene encoding a new H3 histone varian (H3L) has been identified in P. lividus sea urchin embryo. It encodes a H3 histone protein showing the S.//.A.IG amino acid motif typical of the replication independent H3.3 variants but in a mRNA showing the 3' terminal stem-loop nucleotide sequence that is typical of the...

journal_title：FEBS letters

authors： Fucci L,Forte A,Mancini P,Affaitati A,Branno M,Aniello F,Geraci G

更新日期：1997-04-21 00:00:00

abstract：:Dun1p and Rad53p of the budding yeast Saccharomyces cerevisiae are members of a conserved family of cell cycle checkpoint protein kinases that contain forkhead-associated (FHA) domains. Here, we demonstrate that these FHA domains contain 130-140 residues, and are thus considerably larger than previously predicted by s...

journal_title：FEBS letters

authors： Hammet A,Pike BL,Mitchelhill KI,Teh T,Kobe B,House CM,Kemp BE,Heierhorst J

更新日期：2000-04-14 00:00:00

abstract：:Cystatins A and C were both shown to inhibit cathepsin B by a two-step mechanism, involving an initial weak interaction followed by a conformational change. Disruption of the major salt bridge anchoring the occluding loop of cathepsin B to the main body of the enzyme by mutation of His110 to Ala converted the binding ...

journal_title：FEBS letters

authors： Pavlova A,Krupa JC,Mort JS,Abrahamson M,Björk I

更新日期：2000-12-29 00:00:00

abstract：:DNA methylation has a profound impact on the regulation of gene expression in normal cell development, and aberrant methylation has been recognized as a key factor in the pathogenesis of human diseases such as cancer. The discovery of modified nucleobases arising from 5-methylcytosine (5mC) through consecutive oxidati...

journal_title：FEBS letters

authors： Dietzsch J,Feineis D,Höbartner C

更新日期：2018-06-01 00:00:00

abstract：:(7-methoxycoumarin-4-yl)Acetyl-Pro-Leu-Gly-Leu-(3-[2,4-dinitrophenyl]-L- 2,3-diaminopropionyl)-Ala-Arg-NH2 (Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2) has been synthesised as a fluorogenic substrate for the matrix metalloproteinases. The highly fluorescent 7-methoxycoumarin group is efficiently quenched by energy transfer t...

journal_title：FEBS letters

authors： Knight CG,Willenbrock F,Murphy G

更新日期：1992-01-27 00:00:00

abstract：:Subcellular fractionation revealed that a significant fraction of total sphingosine kinase, the enzyme that phosphorylates sphingosine to form the bioactive lipid metabolite sphingosine-1-phosphate, resides in the nuclei of Swiss 3T3 cells, localized to both the nuclear envelope and the nucleoplasm. Platelet-derived g...

journal_title：FEBS letters

authors： Kleuser B,Maceyka M,Milstien S,Spiegel S

更新日期：2001-08-10 00:00:00