Chemoselective labeling and site-specific mapping of 5-formylcytosine as a cellular nucleic acid modification.

abstract：:Helical filaments of bacterial flagella are built up by a self-assembly process from thousands of flagellin subunits. To clarify how the disordered terminal regions of flagellin interact upon filament formation, polymerization ability of various terminally truncated fragments was investigated. Fragments deprived of 19...

journal_title：FEBS letters

authors： Gugolya Z,Muskotál A,Sebestyén A,Diószeghy Z,Vonderviszt F

更新日期：2003-01-30 00:00:00

abstract：:Proline-rich tyrosine kinase2 (Pyk2) is a cytoplasmic tyrosine kinase related to focal adhesion kinase. Pyk2 expression has been known to be restricted to neuronal and hematopoietic cells and Pyk2 tyrosine phosphorylation and its kinase activity is important for the function of monocytes/macrophages. In NB4 acute prom...

journal_title：FEBS letters

authors： Park MH,Park SY,Kim Y

更新日期：2008-02-06 00:00:00

abstract：:An antibody recognising acetylated core histones was used to immunoprecipitate chromatin fragments from proliferating human K562 cells and from cells induced to differentiate with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). The DNA of the acetylated chromatin was probed with sequences of platelet der...

journal_title：FEBS letters

authors： Clayton AL,Hebbes TR,Thorne AW,Crane-Robinson C

更新日期：1993-12-20 00:00:00

abstract：:We propose a dynamic model for the competition between exon-exon ligation and 3'-end hydrolysis valid for sequentially folded pre-mRNA introns of group I. This model accounts for the delay in the formation of conserved helix P10 until the 5' exon has been cleaved, a requirement to prevent hydrolysis at the 3' end of t...

journal_title：FEBS letters

authors： Fernández A

更新日期：1992-02-03 00:00:00

abstract：:The solution structure of endothelin-1, a newly discovered potent bicyclic peptide vaso-constrictor agent, has been investigated using 1H NMR conformational constraints and distance geometry calculations. The conformation is constrained by two disulphide bridges between Cys1-Cys15 and Cys3-Cys11 but the NMR data and c...

journal_title：FEBS letters

authors： Munro S,Craik D,McConville C,Hall J,Searle M,Bicknell W,Scanlon D,Chandler C

更新日期：1991-01-14 00:00:00

abstract：:The relationship between the antioxidant effects of reduced coenzyme Q10 (ubiquinol, UQH2) and vitamin E (alpha-tocopherol) was investigated in beef heart submitochondrial particles in which lipid peroxidation was initiated by incubation with ascorbate + ADP-Fe3+. These effects were examined after extraction of coenzy...

journal_title：FEBS letters

authors： Forsmark P,Aberg F,Norling B,Nordenbrand K,Dallner G,Ernster L

更新日期：1991-07-08 00:00:00

abstract：:The TOE1 gene was discovered as a target of the Egr1 transcription factor that participates in cell growth regulation through the upregulation of p21 and a cell cycle delay at the G2/M phase. We report here that TOE1 is able to bind to the p53 tumor suppressor protein, specifically interacting with the C terminal tetr...

journal_title：FEBS letters

authors： Sperandio S,Tardito S,Surzycki A,Latterich M,de Belle I

更新日期：2009-07-07 00:00:00

abstract：:The glycine-alanine (GA) repeat of the Epstein-Barr virus nuclear antigen-1 inhibits in cis ubiquitin-dependent proteolysis in mammalian cells through a yet unknown mechanism. In the present study we demonstrate that the GA repeat targets an evolutionarily conserved step in proteolysis since it can prevent the degrada...

journal_title：FEBS letters

authors： Heessen S,Dantuma NP,Tessarz P,Jellne M,Masucci MG

更新日期：2003-12-04 00:00:00

abstract：:Electron microscopy of mammalian smooth muscle myosin rods showed them to be 153 +/- 7 nm (SD) long, and to bend sharply (greater than 90 degrees) but infrequently, and pH independently (range 6.5-9.5), at a single site 45 +/- 4 nm from one end of the molecule. Light meromyosin (LMM) preparations were 99 +/- 10 nm lon...

journal_title：FEBS letters

authors： Cross RA

更新日期：1984-10-15 00:00:00

abstract：:Since the discovery of RNAi, its mechanism in plants and animals has been intensively studied, widely exploited as a research tool, and used for a number of potential commercial applications. In this article, we discuss the platforms for delivering RNAi in plants. We provide a brief background to these platforms and c...

journal_title：FEBS letters

authors： Watson JM,Fusaro AF,Wang M,Waterhouse PM

更新日期：2005-10-31 00:00:00

abstract：:A hybrid protein from Neisseria meningitidis, which contains both a peroxiredoxin and a glutaredoxin domain, has been isolated. The enzyme was active in the reduction of various peroxides and dehydroascorbate in the presence of reduced glutathione. These findings suggest that both the peroxiredoxin and glutaredoxin do...

journal_title：FEBS letters

authors： Rouhier N,Jacquot JP

更新日期：2003-11-06 00:00:00

abstract：:Polyamine depletion, obtained in FAO cells with specific inhibitors of biosynthetic enzymes, prevents or decreases the accumulation of hsp 70 mRNA following heat shock [Desiderio et al., Hepatology 24 (1996) 150-156]. The present study shows that under conditions of spermidine depletion caused by alpha-difluoromethylo...

journal_title：FEBS letters

authors： Desiderio MA,Dansi P,Tacchini L,Bernelli-Zazzera A

更新日期：1999-07-16 00:00:00

abstract：:We have characterized the cell surface of zebrafish stratified epithelium using a combined approach of light and atomic force microscopy under conditions which simulate wound healing. Microridges rise on average 100 nm above the surface of living epithelial cells, which correlate to bundles of cytochalasin B-insensiti...

journal_title：FEBS letters

authors： Sharma A,Anderson KI,Müller DJ

更新日期：2005-03-28 00:00:00

abstract：:The malaria parasite has for long been thought to escape host complement attack as a survival strategy. However, it was only recently that complement evasion mechanisms of the parasite were described. Simultaneously, the role of complement in antibody-mediated naturally acquired and vaccine-induced protection against ...

journal_title：FEBS letters

authors： Kiyuka PK,Meri S,Khattab A

更新日期：2020-08-01 00:00:00

abstract：:The nucleotide sequence of the Pseudomonas saccharophila gene encoding maltotetraohydrolase (G4-forming amylase) has been determined. The coding region for the G4-forming amylase precursor contained 1653 nucleotides. The deduced precursor protein included an N-terminal 21-residue putative signal peptide; the deduced m...

journal_title：FEBS letters

authors： Zhou JH,Baba T,Takano T,Kobayashi S,Arai Y

更新日期：1989-09-11 00:00:00

abstract：:The archaeal exosome, a protein complex responsible for phosphorolytic degradation and tailing of RNA, has an RNA-binding platform containing Rrp4, Csl4, and DnaG. Aiming to detect novel interaction partners of the exosome, we copurified Nop5, which is a part of an rRNA methylating ribonucleoprotein complex, with the ...

journal_title：FEBS letters

authors： Gauernack AS,Lassek C,Hou L,Dzieciolowski J,Evguenieva-Hackenberg E,Klug G

更新日期：2017-12-01 00:00:00

abstract：:A variety of plant tissues contain an uncoupling mitochondrial protein (PUMP), recently described and characterized by our group. In this study we show that the inhibition of PUMP activity in potato tuber mitochondria significantly increases mitochondrial H2O2 generation, while PUMP substrates, such as linoleic acid, ...

journal_title：FEBS letters

authors： Kowaltowski AJ,Costa AD,Vercesi AE

更新日期：1998-03-27 00:00:00

abstract：:Recombinant cell lines expressing D2 and D3 dopamine receptor isoforms have been used to study coupling of these receptors to G-proteins from the effects of GTP to reduce agonist binding affinities in agonist/[3H]spiperone competition experiments. D2(short) expressed in Ltk- cells, D2(long) expressed in Ltk- or CHO ce...

journal_title：FEBS letters

authors： Castro SW,Strange PG

更新日期：1993-01-11 00:00:00

abstract：:The half-lives of mRNAs in yeast vary from about 1 to over 100 min. While mRNA stabilities must strongly influence overall gene expression in this organism, very little is known about how they are determined. Labellings of yeast cells were conducted to investigate whether the 5'-cap structures of yeast mRNAs might inf...

journal_title：FEBS letters

authors： Piper PW,Curran B,Davies W,Hirst K,Seward K

更新日期：1987-08-10 00:00:00

abstract：:To assess the role of crystallinity in biological response, we quantitated the generation of reactive oxygen metabolites in human polymorphonuclear leukocytes (PMN) with a chemiluminescence assay using three hydroxyapatite preparations with sintering temperatures of 1,200 degrees C and 900 degrees C and a drying tempe...

journal_title：FEBS letters

authors： Nagase M,Nishiya H,Abe Y

更新日期：1993-07-05 00:00:00

abstract：:Although neurokinin A (NKA), a tachykinin peptide with sequence homology to substance P (SP), is a weak competitor of radiolabeled SP binding to the NK-1 receptor (NK-1R), more recent direct binding studies using radiolabeled NKA have demonstrated an unexpected high-affinity interaction with this receptor. To document...

journal_title：FEBS letters

authors： Bremer AA,Leeman SE,Boyd ND

更新日期：2000-12-01 00:00:00

abstract：:The [Cu(I)-Cu(II)] half-met form of the dinuclear copper site of tyrosinase has been probed by continuous wave electron paramagnetic resonance (EPR) and hyperfine sublevel correlation (HYSCORE) spectroscopy in the presence and absence of inhibitors. In all cases the EPR spectrum is indicative of a d(x(2)-y(2)) ground ...

journal_title：FEBS letters

authors： van Gastel M,Bubacco L,Groenen EJ,Vijgenboom E,Canters GW

更新日期：2000-06-02 00:00:00

abstract：:Understanding specific cargo distribution in differentiated cells is a major challenge. Trafficking kinesin proteins (TRAKs) are kinesin adaptors. They bind the cargo binding domain of kinesin-1 motor proteins forming a link between the motor and their cargoes. To refine the TRAK1/2 binding sites within the kinesin-1 ...

journal_title：FEBS letters

authors： Randall TS,Moores C,Stephenson FA

更新日期：2013-11-29 00:00:00

abstract：:The conformational behavior of cyclic peptides of the amino acid sequence Cys-Phe/Ala-Pro-Ala-Cys has been investigated through the combined use of molecular simulation methods and NMR experiments to find models for beta-VIa turns of proteins. Both oxidized (cyclic) peptides and reduced (linear) forms were investigate...

journal_title：FEBS letters

authors： Weisshoff H,Frost K,Brandt W,Henklein P,Mügge C,Frömmel C

更新日期：1995-09-25 00:00:00

abstract：:The functional molecular masses of the vacuolar membrane H+-ATPase in Saccharomyces cerevisiae under two kinetic conditions for ATP hydrolysis were measured by radiation inactivation. When vacuolar membrane vesicles were exposed to gamma-rays from 60Co, the activities catalyzing a single-cycle and multi-cycles of ATP ...

journal_title：FEBS letters

authors： Hirata R,Ohsumi Y,Anraku Y

更新日期：1989-02-27 00:00:00

abstract：:The interaction of Fas (Apo-1) and TNF receptor-1 with their respective ligands can lead to cell death. The so-called death domain, a sequence motif present in the cytoplasmic portion of the two receptors, has been identified as a critical structural element involved in signal transduction that leads to apoptosis. Her...

journal_title：FEBS letters

authors： Hofmann K,Tschopp J

更新日期：1995-09-11 00:00:00

abstract：:Aspergillus nidulans catalase B (CatB) was purified to homogeneity and characterized as a hydroperoxidase which resembles typical catalases in some physicochemical characteristics: (1) it has an apparent molecular weight of 360000 and is composed of four glycosylated subunits, (2) it has hydrophobic properties as reve...

journal_title：FEBS letters

authors： Calera JA,Sánchez-Weatherby J,López-Medrano R,Leal F

更新日期：2000-06-16 00:00:00

abstract：:Duchenne muscular dystrophy (DMD) is a fatal disorder caused by absence of functional dystrophin protein. Compensation in dystrophin-deficient (mdx) mice may be achieved by overexpression of its fetal paralogue, utrophin. Strategies to increase utrophin levels by stimulating promoter activity using small compounds are...

journal_title：FEBS letters

authors： Perkins KJ,Davies KE

更新日期：2018-06-01 00:00:00

abstract：:Furin, a member of the subtilisin-like pro-protein convertase family, is a type I membrane protein that undergoes ectodomain shedding. Metabolic labeling of cells stably expressing furin demonstrated that the shed form of furin is detected after 30 min. Moreover, sequence analysis revealed that specific residues of th...

journal_title：FEBS letters

authors： Denault J,Bissonnette L,Longpré J,Charest G,Lavigne P,Leduc R

更新日期：2002-09-11 00:00:00

abstract：:Antibodies have been prepared against the 2-oxoglutarate transport proteins purified from bovine heart and rat liver mitochondria. The anti-heart antiserum cross-reacts with the 2-oxoglutarate carrier (OGC) from beef, pig, rat and rabbit heart, but not with the OGC from liver of the same animals. Conversely, the anti-...

journal_title：FEBS letters

authors： Zara V,De Benedittis R,Ragan CI,Palmieri F

更新日期：1990-04-24 00:00:00