Abstract:
:Furin, a member of the subtilisin-like pro-protein convertase family, is a type I membrane protein that undergoes ectodomain shedding. Metabolic labeling of cells stably expressing furin demonstrated that the shed form of furin is detected after 30 min. Moreover, sequence analysis revealed that specific residues of the cysteine-rich region of furin aligned with those of tumor necrosis factor receptor, which is also shed. Introduction within furin's cysteine-rich region of mutations that impair TNFR1 shedding also abolished furin shedding. Our results show that shedding of furin occurs rapidly and further suggest that specific cysteine residues may impart a conformation to the enzyme, thereby affecting its susceptibility to proteolysis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Denault J,Bissonnette L,Longpré J,Charest G,Lavigne P,Leduc Rdoi
10.1016/s0014-5793(02)03249-0keywords:
subject
Has Abstractpub_date
2002-09-11 00:00:00pages
309-14issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579302032490journal_volume
527pub_type
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