Post X-ray crystallographic studies of chymosin: the existence of two structural forms and the regulation of activity by the interaction with the histidine-proline cluster of kappa-casein.

abstract：:We have previously reported that synthetic dsRNA can activate p21 expression by targeting the p21 promoter, thereby suppressing the proliferation of human bladder cancer cells. As complementarity between dsRNA and its target sequences is necessary for RNA activation, miRNAs may also trigger p21 expression through the ...

journal_title：FEBS letters

authors： Wang C,Chen Z,Ge Q,Hu J,Li F,Hu J,Xu H,Ye Z,Li LC

更新日期：2014-12-20 00:00:00

abstract：:Activity of protein phosphatase measured in the absence of divalent cations was decreased by 50% during all-trans retinoic acid (ATRA)-induced HL-60 cell differentiation into the granulocytic phenotype. Treatment of HL-60 cells with ATRA led to a dramatic decrease in the amount of protein phosphatase type 2A (PP2A) pr...

journal_title：FEBS letters

authors： Tawara I,Nishikawa M,Morita K,Kobayashi K,Toyoda H,Omay SB,Shima H,Nagao M,Kuno T,Tanaka C

更新日期：1993-04-26 00:00:00

abstract：:Haloalkane dehalogenase hydrolyses various 1-halo-n-alkanes to the corresponding alcohols by covalent catalysis with formation of an alkyl-enzyme intermediate. The carboxylate function of the nucleophilic aspartate (Asp-124) that displaces the halogen during formation of the intermediate was changed to an amide by sit...

journal_title：FEBS letters

authors： Pries F,Kingma J,Janssen DB

更新日期：1995-01-23 00:00:00

abstract：:Plasma membrane, sarco-endoplasmic reticulum and secretory pathway Ca2+-ATPases (designated PMCA, SERCA and SPCA) regulate intracellular Ca2+ in animal cells. The presence of PMCA, and the absence of SERCA, in sea urchin sperm is known. By using inhibitors of Ca2+-ATPases, we now show the presence of SPCA and Ca2+ sto...

journal_title：FEBS letters

authors： Gunaratne HJ,Vacquier VD

更新日期：2006-07-10 00:00:00

abstract：:The C-terminal regions of Kv1 K+ channels show little conservation between isoforms except for the last four C-terminal residues, (E/L)TDV, which are well conserved from Drosophila to man. Deletions of the 4, 16, and 57 C-terminal residues of the human Kv1.5 channel did not affect whole cell current amplitude, midpoin...

journal_title：FEBS letters

authors： Uebele VN,Yeola SW,Snyders DJ,Tamkun MM

更新日期：1994-02-28 00:00:00

abstract：:A method was established for measuring molecule numbers of three different myosin heavy chain (MHC) mRNA isoforms in total RNA preparations. The quantification was based on a combination of primer-directed reverse transcriptase and polymerase chain reactions with 5'-digoxigenin-labeled oligonucleotides, using external...

journal_title：FEBS letters

authors： Peuker H,Pette D

更新日期：1993-03-08 00:00:00

abstract：:HBV particles with genome derived from spliced mRNAs accumulate in patients with virus-derived severe liver necrosis and fibrosis. We investigated the role of an intronic element (intronic splicing silencer-long, ISS(L)) on splicing of HBV minigene transcripts. Removal of the entire ISS(L) showed two-fold increase in ...

journal_title：FEBS letters

authors： Chowdhury JB,Roy D,Ghosh S

更新日期：2011-10-20 00:00:00

abstract：:Oncostatin M (OSM) is a potent modulator of human lung-derived epithelial cell function. This cytokine binds two distinct receptor complexes: type I OSM receptor which is also a functional receptor for leukemia inhibitory factor (LIF), and type II OSM-specific receptor. The role of these two distinct receptors in medi...

journal_title：FEBS letters

authors： Cichy J,Rose-John S,Puré E

更新日期：1998-06-16 00:00:00

abstract：:The human gut microbiome refers to a highly diverse microbial ecosystem, which has a symbiotic relationship with the host. Molecular hydrogen (H2 ) and carbon dioxide (CO2 ) are generated by fermentative metabolism in anaerobic ecosystems. H2 generation and oxidation coupled to CO2 reduction to methane or acetate help...

journal_title：FEBS letters

authors： Hylemon PB,Harris SC,Ridlon JM

更新日期：2018-06-01 00:00:00

abstract：:The Escherichia coli K12 mutant gene, asnS40, coding for asparaginyl-tRNA synthetase (AsnRS) in the temperature-sensitive strain HO202, was isolated from genomic DNA using the Polymerase Chain Reaction. DNA sequencing revealed that the mutant enzyme differs from the wild-type AsnRS by two amino acids, but only the P23...

journal_title：FEBS letters

authors： Madern D,Anselme J,Härtlein M

更新日期：1992-03-24 00:00:00

abstract：:We report here the molecular cloning, nucleotide sequence, and predicted amino acid sequence of an alpha-subunit of the developmentally useful model, Artemia. The amino acid sequence shows divergence from that of mammals, birds, Torpedo, and Drosophila. However, regions in the putative ATP binding and transmembrane do...

journal_title：FEBS letters

authors： Baxter-Lowe LA,Guo JZ,Bergstrom EE,Hokin LE

更新日期：1989-10-23 00:00:00

abstract：:Osteogenesis depends on a coordinated network of transcription factors including Sp7. Emerging evidence indicates that microRNAs (miRNAs) act as pivotal regulators in various biological processes including osteoblast proliferation and differentiation. Here, we investigated the effect of miR-145 on osteogenic different...

journal_title：FEBS letters

authors： Jia J,Tian Q,Ling S,Liu Y,Yang S,Shao Z

更新日期：2013-09-17 00:00:00

abstract：:Disruption of yeast spheroplasts by DEAE-dextran in isoosmotic conditions allows isolation of relatively undamaged subcellular fractions from yeast. The preservation of mitochondria and vacuoles permits the simultaneous isolation of the cytosol with negligible contamination by vacuolar proteinases and therefore, virtu...

journal_title：FEBS letters

authors： Schwencke J,Canut H,Flores A

更新日期：1983-06-13 00:00:00

abstract：:Fourteen truncated forms of Leuconostoc mesenteroides NRRL B512-F dextransucrase, involving N-, C- or N- plus C-terminal domain truncations were tested for their ability to bind dextrans. The shortest fragment (14kDa molecular weight) that still exhibited a strong interaction with dextran was localized between amino a...

journal_title：FEBS letters

authors： Suwannarangsee S,Moulis C,Potocki-Veronese G,Monsan P,Remaud-Simeon M,Chulalaksananukul W

更新日期：2007-10-02 00:00:00

abstract：:We report the molecular analysis of the transthyretin gene in a large Italian pedigree with familial amyloidotic polyneuropathy and demonstrate the presence of a Met30 mutation. The usefulness of the genetic analysis in the identification of presymptomatic persons and the diagnosis of individuals with partial symptoms...

journal_title：FEBS letters

authors： Strocchi P,Tang XM,Cambi F

更新日期：1995-02-13 00:00:00

abstract：:Lipid metabolism is extensively studied in Saccharomyces cerevisiae. Here, we report that revertant of glycogen synthase kinase mutation-1 (Rog1p) possesses monoacylglycerol (MAG) lipase activity in S. cerevisiae. The lipase activity of Rog1p was confirmed in two ways: through analysis of a strain with a double deleti...

journal_title：FEBS letters

authors： Vishnu Varthini L,Selvaraju K,Srinivasan M,Nachiappan V

更新日期：2015-01-02 00:00:00

abstract：:The dynamic properties of 111 backbone HN sites in uncomplexed human profilin, a protein of 139 residues, have been characterized by two-dimensional inverse-detected 1H-15N NMR spectroscopy. Heteronuclear (1H)-15N nuclear Overhauser effects and 15N longitudinal and transverse relaxation rates have been analyzed in ter...

journal_title：FEBS letters

authors： Constantine KL,Friedrichs MS,Bell AJ,Lavoie TB,Mueller L,Metzler WJ

更新日期：1993-12-28 00:00:00

abstract：:Cap-independent translation (CIT) occurs at the leader sequences of uncapped plant viral RNAs, but also at a number of normally capped cellular mRNAs and has been correlated with sequence complementarity to 18S rRNA. The ribosomal protein S18 (RPS18) is a component of the small ribosomal subunit and is encoded by thre...

journal_title：FEBS letters

authors： Vanderhaeghen R,De Clercq R,Karimi M,Van Montagu M,Hilson P,Van Lijsebettens M

更新日期：2006-05-15 00:00:00

abstract：:Synaptotagmin I, the calcium sensor for neurotransmission, is palmitoylated. We have identified the palmitoylation sites as five cysteine residues located between the transmembrane and cytoplasmic regions. In contrast to wild-type synaptotagmin, the non-acylated mutant is not converted to the endoglycosidase-H-resista...

journal_title：FEBS letters

authors： Heindel U,Schmidt MF,Veit M

更新日期：2003-06-05 00:00:00

abstract：:Xanthine oxidase was decreased 2- to 10-fold in all examined rat hepatomas irrespective of the malignancy; growth rate and degrees of histological differentiation of the neoplasms. The affinity to substrate (KM=6-8 muM) and the pH optimum (8.0) of the liver and hepatoma enzymes were the same. The reprogramming of gene...

journal_title：FEBS letters

authors： Prajda N,Weber G

更新日期：1975-11-15 00:00:00

abstract：:The freshwater ciliate Tetrahymena pyriformis is an ubiquitous organism that is present in all aquatic ecosystems. This protozoan showed a clear resistance against some polycyclic aromatic hydrocarbons which can be attributed to an efflux pump probably of the multidrug resistance (MDR) type. Immunocytochemical detecti...

journal_title：FEBS letters

authors： Bamdad M,Brousseau P,Denizeau F

更新日期：1999-08-13 00:00:00

abstract：:Epidemiological studies testing the effect of beta-carotene in humans have found a relative risk for lung cancer in smokers supplemented with beta-carotene. We investigated the reactions of retinal and beta-apo-8'-carotenal, two beta-carotene oxidation products, with 2'-deoxyguanosine to evaluate their DNA damaging po...

journal_title：FEBS letters

authors： Marques SA,Loureiro AP,Gomes OF,Garcia CC,Di Mascio P,Medeiros MH

更新日期：2004-02-27 00:00:00

abstract：:FITC-labeled LPS from Neisseria meningitidis can be used as a probe to follow the process of LPS incorporation into liposomal membrane and to study its interaction with a bilayer. The incorporation of FITC-LPS into the bilayer was proved by physicochemical methods as well as by liposomal LPS toxicity decrease in actin...

journal_title：FEBS letters

authors： Trubetskoy VS,Koshkina NV,Omel'yanenko VG,L'vov VL,Dmitriev BA,Petrov AB,Torchilin VP

更新日期：1990-08-20 00:00:00

abstract：:We investigated 38 cases of B-cell chronic lymphocytic leukemia (B-CLL) for the presence of non-productive rearrangements in the S(mu) regions and defined for the first time the molecular nature of these rearrangements. Southern blot analysis revealed S(mu) region rearrangements in 13 cases (34%) and polymerase chain ...

journal_title：FEBS letters

authors： Nardini E,Rizzi S,Capello D,Vitolo U,Gaidano G,Menard S,Balsari A

更新日期：2002-05-08 00:00:00

abstract：:The action of substrate analogs containing the tetrazolyl group instead of the C-terminal carboxy group on the peptidase activity of carboxypeptidase A is studied. The analogs compete with the substrate for the secondary binding site thus showing activation phenomena. ...

journal_title：FEBS letters

authors： Kojro E,Willhardt I,Römbach A,Grzonka Z,Hermann P

更新日期：1987-02-09 00:00:00

abstract：:Hell's Gate globin I (HGbI), a heme-containing protein structurally homologous to mammalian neuroglobins, has been identified from an acidophilic and thermophilic obligate methanotroph, Methylacidiphilum infernorum. HGbI has very high affinity for O(2) and shows barely detectable autoxidation in the pH range of 5.2-8....

journal_title：FEBS letters

authors： Teh AH,Saito JA,Baharuddin A,Tuckerman JR,Newhouse JS,Kanbe M,Newhouse EI,Rahim RA,Favier F,Didierjean C,Sousa EH,Stott MB,Dunfield PF,Gonzalez G,Gilles-Gonzalez MA,Najimudin N,Alam M

更新日期：2011-10-20 00:00:00

abstract：:Early in the course of pregnancy, at the preimplantation stage, the pig embryo is likely to exert a paracrine effect on the tissue intended to receive it, via the secretion of interferons. Our observations show that trophoblastic interferons induce an increase of some mRNAs in the epithelial cells of the gilt endometr...

journal_title：FEBS letters

authors： Chwetzoff S,d'Andrea S

更新日期：1997-03-24 00:00:00

abstract：:Platinum electrodes can be coated with cytochrome b5-phospholipid monolayers by the Langmuir-Blodgett technique. Cyclic voltammetry of a series of dyes shows that the coated electrodes become selective for certain electroactive species. The electron transfer reactions of negatively charged species are inhibited at the...

journal_title：FEBS letters

authors： Zaba BN,Wilkinson MC,Taylor DM,Lewis TJ,Laidman DL

更新日期：1987-03-09 00:00:00

abstract：:We report results from automated docking and microscopic molecular dynamics simulations of the tetraethylammonium (TEA) complexes with KcsA. Binding modes and energies for TEA binding at the external and internal sides of the channel pore are examined utilising the linear interaction energy method. Effects of the chan...

journal_title：FEBS letters

authors： Luzhkov VB,Aqvist J

更新日期：2001-04-27 00:00:00

abstract：:Phosphoenolpyruvate carboxylases from various organisms contain two conserved lysine residues. In the C4 dicot Flaveria trinervia, one of these residues is Lys600. Converting this Lys600 to Arg600 or Thr600 mainly increased the Km values and but had minimal effect on the Vmax. The Km for PEP, Mg2+ increased by up to 3...

journal_title：FEBS letters

authors： Gao Y,Woo KC

更新日期：1995-11-13 00:00:00