Abstract:
:Osteogenesis depends on a coordinated network of transcription factors including Sp7. Emerging evidence indicates that microRNAs (miRNAs) act as pivotal regulators in various biological processes including osteoblast proliferation and differentiation. Here, we investigated the effect of miR-145 on osteogenic differentiation. miR-145 was decreased during osteogenic differentiation, which could suppress the osteogenic differentiation of C2C12 and MC3T3-E1 cells confirmed by gain- and loss-of-function experiments. Moreover, bioinformatic analysis combined with luciferase reporter assay, and Western blot validated that miR-145 negatively regulated Sp7 expression. Inhibition of Sp7 showed similar effect with miR-145 on osteogenic differentiation, whereas overexpression of Sp7 attenuated this effect. Collectively, these data indicate that miR-145 is a novel regulator of Sp7, and it suppresses the osteogenic differentiation of C2C12 and MC3T3-E1 cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Jia J,Tian Q,Ling S,Liu Y,Yang S,Shao Zdoi
10.1016/j.febslet.2013.07.030subject
Has Abstractpub_date
2013-09-17 00:00:00pages
3027-31issue
18eissn
0014-5793issn
1873-3468pii
S0014-5793(13)00559-0journal_volume
587pub_type
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