Abstract:
:The fate of newly synthesized human immunodeficiency virus type 1 env gp160 was examined in COS-1 cells. The results of morphological chase experiments involving cycloheximide demonstrated that gp160 was retained in the Golgi apparatus for longer than the half-life of the molecule. The degradation of gp160 was insensitive to both bafilomycin A1 and leupeptin (< 0.2 mM), which block lysosomal proteolysis. However, degradation was effectively suppressed by leupeptin at higher concentrations, maximally at 1.7 mM. Furthermore, undegraded gp160 was accumulated in the Golgi apparatus, but was not detected in lysosomes. These results indicate that in COS-1 cells gp160 is not degraded in lysosomes, but rather that degradation takes place in the Golgi apparatus.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kimura T,Nishikawa M,Fujisawa Jdoi
10.1016/0014-5793(96)00614-xsubject
Has Abstractpub_date
1996-07-15 00:00:00pages
15-20issue
1eissn
0014-5793issn
1873-3468pii
001457939600614Xjournal_volume
390pub_type
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