Abstract:
:Incubation of calf thymus DNA with 3-morpholinosydnonimine (SIN-1), which simultaneously generates nitric oxide (NO) and superoxide (O2-), induced a significant increase of 8-hydroxydeoxyguanosine (8-OH-dG). Peroxynitrite also increased 8-OH-dG in calf thymus DNA. Addition of free hydroxyl radical (.OH) scavengers inhibited the increase of 8-OH-dG by SIN-1 or peroxynitrite. Incubation of 32P-labeled DNA fragment with SIN-1 or peroxynitrite caused DNA cleavage at every nucleotide with a little dominance at guanine residues. The results suggest that NO reacts with O2- to form peroxynitrite and the peroxynitrite induces oxidative DNA damage through an active intermediate of which reactivity is similar to .OH.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Inoue S,Kawanishi Sdoi
10.1016/0014-5793(95)00873-8subject
Has Abstractpub_date
1995-08-28 00:00:00pages
86-8issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(95)00873-8journal_volume
371pub_type
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