Abstract:
:As an attempt to investigate the dynamic interactions between plasma serine protease, coagulation factor VIIa (VIIa) and its cofactor, tissue factor (TF), we performed normal mode analysis (NMA) of the complex of VIIa with soluble TF (the extracellular part of TF; sTF). We compared fluctuations of Calpha atoms of VIIa or sTF derived from NMA in the VIIa-sTF complex with those of VIIa or sTF in an uncomplexed condition. The atomic fluctuations of the Calpha atoms of sTF complexed with VIIa did not significantly differ from those of sTF without VIIa. In contrast, the atomic fluctuations of VIIa complexed with sTF were much smaller than those of VIIa without sTF. These results suggest that domain motions of VIIa molecule alone are markedly dampened in the VIIa-sTF complex and that the sTF molecule is relatively more rigid than the VIIa molecule. This may indicate functions of TF as a cofactor.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Soejima K,Kurihara Y,Kamiya K,Umeyama Hdoi
10.1016/s0014-5793(99)01564-1keywords:
subject
Has Abstractpub_date
1999-12-10 00:00:00pages
19-23issue
1-2eissn
0014-5793issn
1873-3468pii
S0014-5793(99)01564-1journal_volume
463pub_type
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