Diminished AMPK signaling response to fasting in thioredoxin-interacting protein knockout mice.

abstract：:The DNA binding characteristics of a mono-, di- and trimeric derivative of 9-aminoacridine were studied. The length of the linking carboxamidoalkyl chains was selected to allow bis- or tris-intercalation according to the excluded-site model. Measurements of DNA unwinding angle using closed circular DNA showed that the...

journal_title：FEBS letters

authors： Gaugain B,Markovits J,Le Pecq JB,Roques BP

更新日期：1984-04-24 00:00:00

abstract：:Large conductance voltage-dependent and Ca(2+)-modulated K(+) channels play a crucial role in myometrium contractility. Western blots and immunocytochemistry of rat uterine sections or isolated cells show that MaxiK channel protein signals drastically decrease towards the end of pregnancy. Consistent with a transcript...

journal_title：FEBS letters

authors： Song M,Zhu N,Olcese R,Barila B,Toro L,Stefani E

更新日期：1999-11-05 00:00:00

abstract：:Bcl-xL, a close homolog of Bcl2, is an important regulator of apoptosis and is overexpressed in human cancer. Phosphorylation of Bcl-xL can be induced by microtubule-damaging drugs such as taxol or 2-methoxyestradiol (2-ME). By site-directed mutagenesis studies, we have identified that serine 62 is the necessary site ...

journal_title：FEBS letters

authors： Basu A,Haldar S

更新日期：2003-03-13 00:00:00

abstract：:The nuclear factor NF-IL6 had been suggested to be responsible for the IL-6-mediated induction of several acute-phase proteins. To obtain evidence for the involvement of NF-IL6 in the induction of acute-phase proteins, we introduced the NF-IL6 gene and its truncated mutant (delNFIL6) gene into a hepatoma cell line Hep...

journal_title：FEBS letters

authors： Natsuka S,Isshiki H,Akira S,Kishimoto T

更新日期：1991-10-07 00:00:00

abstract：:The gene of ecotin, an E. coli proteinase inhibitor, was cloned, and by site-directed mutagenesis the active site residue of the protein, Met84, was mutated to Lys, Arg and Leu. The recombinant wild-type and mutant inhibitors were overexpressed in E. coli, purified to homogeneity and their inhibitory effects on trypsi...

journal_title：FEBS letters

authors： Pál G,Sprengel G,Patthy A,Gráf L

更新日期：1994-03-28 00:00:00

abstract：:Transmembrane helices (TMHs) in membrane proteins can be orientated with their N-terminus towards the cytoplasm (Nin), or facing the non-cytoplasmic side (Nout). Most membrane proteins are inserted co-translationally into membranes, aided by Sec-type translocons. Since the final orientation of Nin- and Nout-orientated...

journal_title：FEBS letters

authors： Cymer F,Ismail N,von Heijne G

更新日期：2014-05-21 00:00:00

abstract：:Neuritic plaques are the key pathological feature of Alzheimer's disease, and amyloid beta (Abeta) peptides are major component of these plaques. In this study, we demonstrated the influence of aluminum (Al) on the Abeta peptide degradation by cathepsin D. Al did not directly affect the cathepsin D activity using smal...

journal_title：FEBS letters

authors： Sakamoto T,Saito H,Ishii K,Takahashi H,Tanabe S,Ogasawara Y

更新日期：2006-12-11 00:00:00

abstract：:Urate oxidase from Aspergillus flavus is a 135 kDa homo-tetramer which has a hydrophobic cavity buried within each monomer and located close to its active site. Crystallographic studies under moderate gas pressure and high hydrostatic pressure have shown that both gas presence and high pressure would rigidify the cavi...

journal_title：FEBS letters

authors： Colloc'h N,Prangé T

更新日期：2014-05-02 00:00:00

abstract：:Azotobacter vinelandii (Av) and chroococcum (Ac) ferredoxin I contain [3Fe-4S]1 + 0 and [4Fe-4S]2+1+ clusters, when isolated aerobically, which undergo one-electron redox cycles at potentials of -460 +/- 10 mV (vs SHE) at pH 8.3 and -645 +/- 10 mV, respectively. The X-ray structure of Fd I (Av) reveals that the N-term...

journal_title：FEBS letters

authors： Thomson AJ

更新日期：1991-07-22 00:00:00

abstract：:In the central nervous system, synaptic signal transduction depends on the regulation of neurotransmitter receptors by interacting proteins. Here, we searched for proteins interacting with two metabotropic glutamate receptor type 8 isoforms (mGlu8a and mGlu8b) and identified RanBPM. RanBPM is expressed in several brai...

journal_title：FEBS letters

authors： Seebahn A,Rose M,Enz R

更新日期：2008-07-09 00:00:00

abstract：:Epidermal growth factor (EGF) induces transformed phenotypes in EGF receptor-overexpressing NIH3T3 (ER12) cells. Tyrosine kinase inhibitors such as erbstatin and its stable analogue methyl 2,5-dihydroxycinnamate inhibited the EGF-induced phenotypic changes in these cells; while 5'-O-methylerbstatin, an inactive analog...

journal_title：FEBS letters

authors： Umezawa K,Sugata D,Yamashita K,Johtoh N,Shibuya M

更新日期：1992-12-21 00:00:00

abstract：:Energetic behaviors of eight pairs of RNA/DNA hybrid duplexes with identical nearest neighbors have been investigated by UV melting analysis. In the pairs with identical nearest-neighbor pairs, the melting curve traces at the same strand concentration were very similar. The average difference in stabilization energy o...

journal_title：FEBS letters

authors： Sugimoto N,Katoh M,Nakano S,Ohmichi T,Sasaki M

更新日期：1994-10-31 00:00:00

abstract：:Respiration in all cells depends upon synthesis of ATP by the ATP synthase complex, a rotary motor enzyme. The structure of the catalytic moiety of ATP synthase, the so-called F(1) headpiece, is well established. F(1) is connected to the membrane-bound and ion translocating F(0) subcomplex by a central stalk. A periph...

journal_title：FEBS letters

authors： Dudkina NV,Heinemeyer J,Keegstra W,Boekema EJ,Braun HP

更新日期：2005-10-24 00:00:00

abstract：:Human milk cholesterol esterase (bile salt-activated lipase) plays a role in the dietary uptake of triacylglyceride and cholesteryl ester. The activities toward these substrates are mediated through a unique bile salt-activated mechanism. Previously, it has been proposed that a necessary step in this process is prior ...

journal_title：FEBS letters

authors： Loomes KM,Senior HE

更新日期：1997-04-01 00:00:00

abstract：:Based on the amino acid alignment, Caenorhabditis elegans F32D1.1 was identified to be a homologue of the mammalian fidgetin. We produced and purified the F32D1.1 protein by using a baculovirus-expression system. F32D1.1 has an ATPase activity, which is sensitive to N-ethylmaleimide. Km and Vmax for the ATPase activit...

journal_title：FEBS letters

authors： Yakushiji Y,Yamanaka K,Ogura T

更新日期：2004-12-03 00:00:00

abstract：:Botulinum neurotoxins (BoNTs), the causative agent of human botulism, are the most potent naturally occurring toxins known. BoNT/A1, the most studied BoNT, is also used as an important biopharmaceutical. In this study, the biological activity of BoNT/A1 is compared to that of BoNT/A2 using neuronal cell models. The da...

journal_title：FEBS letters

authors： Pier CL,Chen C,Tepp WH,Lin G,Janda KD,Barbieri JT,Pellett S,Johnson EA

更新日期：2011-01-03 00:00:00

abstract：:The small GTPase Rho1 plays an essential role in controlling the organization of the actin cytoskeleton and synthesis of the cell wall in the fission yeast Schizosaccharomyces pombe. Here we studied the role of Rho5 whose primary structure is very similar to that of Rho1. It was found that elevated expression of Rho5 ...

journal_title：FEBS letters

authors： Nakano K,Arai R,Mabuchi I

更新日期：2005-09-26 00:00:00

abstract：:VASP (vasodilator-stimulated phosphoprotein), a protein associated with microfilaments at cellular contact sites, has been identified as a ligand for profilin and zyxin, two proteins also involved in microfilament dynamics and organization at these regions. Here, we report that VASP also directly binds to vinculin, an...

journal_title：FEBS letters

authors： Reinhard M,Rüdiger M,Jockusch BM,Walter U

更新日期：1996-12-09 00:00:00

abstract：:Tissue plasminogen activator was purified in high yield from pig heart by immunoaffinity chromatography and characterized by analysis of the glycosylation pattern and the N-terminal amino acid sequence. Comparisons with the human enzyme reveals residue exchanges in the A-chain at positions 3 (porcine Arg/human Gln) an...

journal_title：FEBS letters

authors： Pohl G,Jörnvall H,Kok P,Wallén P

更新日期：1986-09-01 00:00:00

abstract：:The intestinal epithelium is equipped with sensing receptor mechanisms that interact with luminal microorganisms and nutrients to regulate barrier function and gut immune responses, thereby maintaining intestinal homeostasis. Herein, we clarify the role of the extracellular calcium-sensing receptor (CaSR) using intest...

journal_title：FEBS letters

authors： Cheng SX,Lightfoot YL,Yang T,Zadeh M,Tang L,Sahay B,Wang GP,Owen JL,Mohamadzadeh M

更新日期：2014-11-17 00:00:00

abstract：:Caloric restriction (CR) is known to promote longevity in various species. Sirtuin-mediated deacetylation has been shown to be related to the promotion of longevity in some species. Here, we show that CR of rats led to an increase in the level of Werner syndrome protein (WRN), a recognized DNA repair protein. In addit...

journal_title：FEBS letters

authors： Kahyo T,Mostoslavsky R,Goto M,Setou M

更新日期：2008-07-23 00:00:00

abstract：:To address biophysical principles and lipid interactions that underlie the properties of membrane proteins, modifications that vary the neighbors of tryptophan residues in the highly dynamic transmembrane helix of GW4,20 ALP23 (acetyl-GGAW4 A(LA)6 LAW20 AGA-amide) were examined using deuterium NMR spectroscopy. It was...

journal_title：FEBS letters

authors： McKay MJ,Greathouse DV,Koeppe RE 2nd

更新日期：2020-12-01 00:00:00

abstract：:We have previously published a simple kinetic model to analyse possible pitfalls in kinetic measurements of H+/O ratios in mitochondria [(1984) FEBS Lett. 178, 187-192]. While this model demonstrated how relative electrode response times may affect the results, it did not adequately describe the kinetics of proton bac...

journal_title：FEBS letters

authors： Krab K,Wikström M

更新日期：1986-06-09 00:00:00

abstract：:Phosphatidylinositol 3 kinase (PI-3 kinase) activity has been linked to cell proliferation and growth regulation. Therefore, we studied changes in phosphoinositide metabolism during the cell cycle of HT-29 cells, a colonic epithelial cell line. HT29 cells were treated with the microtubule disrupter, nocodozole, separa...

journal_title：FEBS letters

authors： Dobos GJ,Wu XR,Traynor-Kaplan A

更新日期：1993-06-14 00:00:00

abstract：:A microsomal fraction prepared from the mycelia of the band (bd) strain of Neurospora crassa showed enhanced phosphorylation of two small proteins with molecular masses of around 15 kDa (ps15) by the irradiation of the reaction mixture containing [gamma-32P]ATP at 0 degrees C for 1 s with blue light (450 nm, 6 mumol/m...

journal_title：FEBS letters

authors： Oda K,Hasunuma K

更新日期：1994-05-30 00:00:00

abstract：:The primary structure of 20 beta-hydroxysteroid dehydrogenase from Streptomyces hydrogenans was determined after FPLC purification of a commercial preparation. Peptides obtained from different proteolytic cleavages were purified by reverse phase HPLC. The 255-residue structure deduced was found to be distantly homolog...

journal_title：FEBS letters

authors： Marekov L,Krook M,Jörnvall H

更新日期：1990-06-18 00:00:00

abstract：:Previously, several studies have demonstrated that autophosphorylation of the C-terminal tyrosine residues (Tyr1316 and Tyr1322) affects the signaling properties of the insulin receptor in vivo. To assess the biochemical consequences of the C-terminal phosphorylation in vitro, we have constructed, purified and charact...

journal_title：FEBS letters

authors： Tennagels N,Bergschneider E,Al-Hasani H,Klein HW

更新日期：2000-08-11 00:00:00

abstract：:Parathyroid hormone (PTH) secretion is stimulated by low extracellular calcium (Ca2+) in association with a reduction in cyosolic Ca2+, indicating that this cell type does not conform to classical models of stimulus-secretion coupling. We used the phorbol ester TPA (12-O-tetradecanoyl phorbol 13-acetate), which direct...

journal_title：FEBS letters

authors： Brown EM,Redgrave J,Thatcher J

更新日期：1984-09-17 00:00:00

abstract：:A Bacillus subtilis strain deficient in seven extracellular proteases was used to produce human mast cell chymase and is a viable expression system for serine proteases and other classes of proteins. Chymase is produced at 0.3-0.5 mg/l and is purified by three chromatography steps. Two crystal forms of PMSF-treated ch...

journal_title：FEBS letters

authors： McGrath ME,Osawa AE,Barnes MG,Clark JM,Mortara KD,Schmidt BF

更新日期：1997-08-25 00:00:00

abstract：:Thimet oligopeptidase (EC 3.4.24.15; TOP) is a Zn(II) endopeptidase implicated in physiological regulation of processes involving neuropeptides. The present study clarifies the active site structure and mechanism of catalysis of TOP. The enzyme exhibited a bell-shaped pH dependence of activity having an acidic limb du...

journal_title：FEBS letters

authors： Sigman JA,Edwards SR,Pabon A,Glucksman MJ,Wolfson AJ

更新日期：2003-06-19 00:00:00