Abstract:
:Based on the amino acid alignment, Caenorhabditis elegans F32D1.1 was identified to be a homologue of the mammalian fidgetin. We produced and purified the F32D1.1 protein by using a baculovirus-expression system. F32D1.1 has an ATPase activity, which is sensitive to N-ethylmaleimide. Km and Vmax for the ATPase activity of F32D1.1 were estimated to be 0.44 mM and 225 nmol/mg/min, respectively. When the cysteine at the position of 368 was mutated to alanine, the ATPase activity was greatly decreased; Vmax was decreased to one-sixth, while Km remained similar. These results suggest that the unique position of cysteine 368, located immediately downstream of the Walker A motif, plays an important role in the ATP hydrolysis process of C. elegans F32D1.1 protein.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Yakushiji Y,Yamanaka K,Ogura Tdoi
10.1016/j.febslet.2004.11.009keywords:
subject
Has Abstractpub_date
2004-12-03 00:00:00pages
191-7issue
1-2eissn
0014-5793issn
1873-3468pii
S0014-5793(04)01371-7journal_volume
578pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::We have isolated four lambda-phages covering the complete pig aminopeptidase N/CD13 gene. The sequence of 2.85 kbp encompasses 1.18 kbp of the 5' upstream region and 1.67 kbp of the structural gene. In the promoter region we find a TATA box and potential binding sites for CTF-1/NF-1 and AP-2. By sequence comparisons w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81470-x
更新日期:1989-07-17 00:00:00
abstract::Two synthetic photoactive azidoarylphosphatidylcholines were used to investigate the level of interaction between D-beta-hydroxybutyrate apodehydrogenase (apoBDH), an amphipathic membrane protein, with the hydrophobic domain of phospholipids. The two synthetic lecithins, PL I (1-myristoyl-2-12-N-(4-azido-2-nitrophenyl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81178-9
更新日期:1985-03-11 00:00:00
abstract::We have recently reported a neural variant of microtubule-associated protein 4 with a short pro-rich region (MAP4-SP). Here, we show that the neural MAP4 has reduced microtubule-stabilizing activity, compared to the ubiquitous MAP4 with a long pro-rich region (MAP4-LP), both in vitro and in vivo. Fluorescence recovery...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.05.028
更新日期:2006-06-12 00:00:00
abstract::Increasingly available genomic sequencing data are exploited to identify genes and variants contributing to diseases, particularly cancer. Traditionally, methods to find such variants have relied heavily on allele frequency and/or familial history, often neglecting to consider any mechanistic understanding of their fu...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12988
更新日期:2018-02-01 00:00:00
abstract::Chromatography of the soluble porcine lymphocyte phospholipase C on cellulose phosphate resolves the activity into two species. An HPLC method is described for separating the enzyme products, Ins 1,2 greater than P and Ins 1P. Use of these methods reveals that the two iso-enzymes liberate, with a high degree of reprod...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)81380-1
更新日期:1986-08-11 00:00:00
abstract::The YukD protein in Bacillus subtilis was identified in a hidden Markov model (HMM) search as being related in sequence to ubiquitin. By solving the crystal structure we show that YukD adopts a fold that is most closely related to ubiquitin, yet has the shortest C-terminal tail of all known ubiquitin-like proteins. Th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.06.002
更新日期:2005-07-04 00:00:00
abstract::JAK2 V617F mutant induces transformation through aberrant activation of various transcription factors. We found that the expression of Aurora kinase A (Aurka) was significantly induced by mutant JAK2 through c-Myc expression. Interestingly, mutant JAK2 enhanced resistance to cisplatin (CDDP)-induced DNA damage, and ef...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.04.068
更新日期:2011-06-23 00:00:00
abstract::Recent evidence indicates that cell-cycle regulating proteins are involved in apoptotic process in post-mitotic neurons. In this study, we examined cell-cycle regulators for G1/S cell-cycle progression after a transient focal cerebral ischemia induced by middle cerebral artery (MCA) occlusion. In the cerebral frontopa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.07.028
更新日期:2005-08-29 00:00:00
abstract::Pheromone receptors are expressed in the accessory olfactory system, which is vital for non-specific chemical communication and for sexual behavior. Under the hypothesis that some of the pheromone molecules released from female reproductive organs might regulate sperm chemotaxis or chemokinesis, we examined whether th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02411-x
更新日期:2001-01-19 00:00:00
abstract::It is known that a high incidence of hepatocellular carcinoma in rat liver can be induced by such azo dye carcinogens as 3'-methyl-4-dimethylaminoazobenzene (3'-MeDAB). Mammalian S-adenosylmethionine (AdoMet) synthetase exists as two isozymes, non-hepatic-type and liver-type enzymes, which are the products of two diff...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81682-p
更新日期:1993-11-08 00:00:00
abstract::CDK11(p58), a 58kDa protein of the PITSLRE kinase family, plays an important role in cell cycle progression, and is closely related to cell apoptosis. To gain further insight into the function of CDK11(p58), we screened a human fetal liver cDNA library for its interacting proteins using the yeast two-hybrid system. He...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.05.039
更新日期:2005-07-04 00:00:00
abstract::We have previously identified the binding region of a new Ca2+ antagonist semotiadil in the skeletal muscle Ca2+ channel. To the same semotiadil derivatives, the cardiac counterpart showed distinct and different binding characteristics: semotiadil and its photoaffinity analog D51-4700 inhibited [3H]PN200-110 binding t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01536-1
更新日期:1998-12-11 00:00:00
abstract::NG-Methyl-L-arginine (L-NMA) and NG-nitro-L-arginine (L-NNA) inhibited NO-induced cGMP accumulation in porcine aortic endothelial cells with half-maximally effective concentrations of 15 and 3.4 microM, respectively. The effects of both compounds were reversible, but the L-NNA-induced inhibition was only reversed by w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80405-j
更新日期:1993-10-25 00:00:00
abstract::The binding of glycosaminoglycans to a synthetic peptide (SKAQKAQAKQAKQAQKAQKAQAKQAKQW-CONH(2)), consisting of a hybrid consensus heparin binding sequence, is studied using circular dichroism, fluorescence anisotropy and nuclear magnetic resonance techniques. The results unveil certain novel features, most importantly...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01964-5
更新日期:2000-09-29 00:00:00
abstract::Increased expression of low voltage-activated, T-type Ca(2+) channels has been correlated with a variety of cellular events including cell proliferation and cell cycle kinetics. The recent cloning of three genes encoding T-type alpha(1) subunits, alpha(1G), alpha(1H) and alpha(1I), now allows direct assessment of thei...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01832-9
更新日期:2000-07-28 00:00:00
abstract::In contrast to the preponderance of affected males in families with X-linked mental retardation, Rett syndrome (RTT) is a neurological disorder occurring almost exclusively in females. The near complete absence of affected males in RTT families has been explained by the lethal effect of an X-linked gene mutation in he...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01994-3
更新日期:2000-09-22 00:00:00
abstract::The structure and organization of five genes responsible for the synthesis of six isoforms of short-chain alpha-neurotoxins in Pseudonaja textilis venom are reported in this paper. This also forms the first report which describes the synthesis of two neurotoxin mRNA variants from one of these genes (Pt-sntx1) as a res...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01549-0
更新日期:2000-05-19 00:00:00
abstract::In isolated rat liver perfused at constant flow with erythrocyte-free Krebs-Henseleit bicarbonate buffer containing 5 mM glucose and 2 mM lactate, perivascular stimulation of the hepatic nerves caused a rapid decrease of oxygen uptake, a decrease of the periportal and, after a transient rise, of the perivenous tissue ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80844-3
更新日期:1984-02-13 00:00:00
abstract::CD38, a type II transmembrane glycoprotein, behaves as a catalytically active transporter responsible for ectocellular generation of cyclic ADP-ribose (cADPR) from NAD+ and for subsequent influx of cADPR across membranes [Franco, L., Guida, L., Bruzzone, S., Zocchi, E., Usai, C. and De Flora, A. (1998) FASEB J. in pre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00929-6
更新日期:1998-08-21 00:00:00
abstract::In human platelet membranes enhanced tyrosine phosphorylation of certain proteins was observed when Zn2+ instead of Mg2+ or Mn2+ was used as a divalent cation for the kinase reaction. An enhanced level of phosphate incorporation into tyrosine residues occurred into a 68 kDa polypeptide besides the 45 kDa and 105 kDa p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81232-8
更新日期:1988-08-01 00:00:00
abstract::The release of a GPI-linked oncofetal protein (PLAP) from HEp-2 cells has been studied as an indicator of endogenous PI-specific PLC activation. A non-hydrolysable GTP analogue, EGF and insulin all produced a dose-dependent release of PLAP from these cells. This indicates that an increase in PLC activity following eit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80927-b
更新日期:1990-07-16 00:00:00
abstract::We have generated a mouse model for hepatic ischemia in which surgical subcutaneous transposition of the spleen allows hepatic ischemia to be applied without affecting other tissues. Using this mouse model we investigated the relationship between the length of ischemic periods in the liver and subsequent liver functio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01517-2
更新日期:1997-12-29 00:00:00
abstract::The plant hormone abscisic acid (ABA) regulates important developmental and stress responses. Recent data show that ABA activates phosphorylation events, but whether dephosphorylation events are post-translationally regulated by ABA or whether these are constitutive remains unknown. Slow anion channels in the plasma m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00526-2
更新日期:1998-05-29 00:00:00
abstract::The level of reduction of cytochrome a and CuA during the oxidation of ferrocytochrome c has been determined in stopped-flow experiments. Both components are partially reduced but become progressively more oxidized as the reaction proceeds. When all cytochrome c has been oxidized, CuA is also completely oxidized, wher...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80040-0
更新日期:1986-01-01 00:00:00
abstract::X-linked inhibitor of apoptosis protein (XIAP) is a potent antagonist of caspases, and functions as a ubiquitin-E3 ligase by itself and for caspases. Recently, NEDD8, a ubiquitin-like modifier, has been suggested to be used for modification of caspase-7 mediated by XIAP. However, it is not clear whether caspase-7 is a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.04.056
更新日期:2012-06-04 00:00:00
abstract::Golgin45 plays important roles in Golgi stack assembly and is known to bind both the Golgi stacking protein GRASP55 and Rab2 in the medial-Golgi cisternae. In this study, we sought to further characterize the cisternal adhesion complex using a proteomics approach. We report here that Acyl-CoA binding domain containing...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12780
更新日期:2017-09-01 00:00:00
abstract::It is shown that conformational changes of receptor proteins brought about by binding of a ligand induce changes in the lipid environment of the receptor that can be monitored by fluorescent lipid probes. On this basis a new approach to studies of ligand-receptor binding is proposed. Using the interaction of the ricin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80108-9
更新日期:1986-01-06 00:00:00
abstract::Retrovirus genomic mRNA exhibits a several hundred nucleotides-long untranslated region (5' UTR) which encloses many control elements required for retrovirus replication. In addition, this 5' UTR contains translation regulatory elements, such as internal ribosome entry sites (IRESes) that have been described in oncore...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02137-8
更新日期:2001-02-09 00:00:00
abstract::The 21-residue peptide KLLLLKLLLLKLLLLKLLLLK (KL4) has been synthesized and analyzed regarding its secondary structure and orientation in lipid environments. Fourier transform infrared and circular dichroism spectroscopy shows that the peptide exhibits approximately 80% alpha-helical content both in dodecylphosphochol...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00290-6
更新日期:1996-04-15 00:00:00
abstract::Methanosarcina barkeri inserts pyrrolysine (Pyl) at an in-frame UAG codon in its monomethylamine methyltransferase gene. Pyrrolysyl-tRNA synthetase acylates Pyl onto tRNAPyl, the amber suppressor pyrrolysine Pyl tRNA. Here we show that M. barkeri Fusaro tRNAPyl can be misacylated with serine by the M. barkeri bacteria...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.08.027
更新日期:2008-10-15 00:00:00