Abstract:
:X-linked inhibitor of apoptosis protein (XIAP) is a potent antagonist of caspases, and functions as a ubiquitin-E3 ligase by itself and for caspases. Recently, NEDD8, a ubiquitin-like modifier, has been suggested to be used for modification of caspase-7 mediated by XIAP. However, it is not clear whether caspase-7 is a bona fide target for NEDD8. Here we showed that no neddylation of caspase-7 but that of XIAP itself was observed under the conditions in which caspase-7 was modified with ubiquitin. These results reveal that XIAP does not function as a NEDD8-E3 ligase for caspase-7 in vivo.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Nagano T,Hashimoto T,Nakashima A,Kikkawa U,Kamada Sdoi
10.1016/j.febslet.2012.04.056subject
Has Abstractpub_date
2012-06-04 00:00:00pages
1612-6issue
11eissn
0014-5793issn
1873-3468pii
S0014-5793(12)00359-6journal_volume
586pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The cloning and analysis of a cDNA clone encoding the soybean metalloproteinase obtained by polymerase chain reaction (PCR) and the rapid amplification of cDNA ends (RACE) reaction are described. The cDNA was constructed from poly(A)+ RNA isolated from 15-17 day old leaves. The deduced amino acid sequence of the cDNA ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00141-5
更新日期:1997-03-10 00:00:00
abstract::The 500 MHz 1H NMR spectrum of a 68-residue peptide, encompassing the rat thyroid transcription factor 1 homeodomain (TTF-1 HD), was fully assigned using standard 2D NMR methodology. The secondary structure elements and their spatial organization were determined and led to a structure very similar to that previously d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80845-l
更新日期:1993-12-28 00:00:00
abstract::In this study, we demonstrated that laminin-1 (LM-1) induces neurite outgrowth and enhances the expression of neurofilament-L and MAP2 in human bone marrow mesenchymal stem cells (MSCs). The c-Jun transcription factor was strongly activated by LM-1 during neurite induction. Suppression of c-Jun inhibited the expressio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.04.072
更新日期:2011-06-23 00:00:00
abstract::Chimeric antibodies composed of mouse-derived variable regions and human-derived constant regions have been developed for clinical use. However, construction of chimeric mouse/human genes in expression vectors is time-consuming work. In this study, we developed convenient vectors for construction of chimeric mouse/hum...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80550-2
更新日期:1989-02-27 00:00:00
abstract::DNA complementary to mRNA extracted from the thyroid glands of patients suffering from medullary carcinoma of the thyroid (MCT), a calcitonin-producing tumour, was inserted in the Pst site of pBR 322 by G-C tailing. The recombinant plasmids were used to transform Escherichia coli DP 50. Ampicillin-resistant clones wer...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80839-x
更新日期:1984-02-13 00:00:00
abstract::Fructose-1,6-bisphosphatase, a key enzyme in gluconeogenesis, undergoes catabolite inactivation when glucose is added to gluconeogenetically active cells of the yeast Saccharomyces cerevisiae. Phosphorylation of the enzyme is followed by rapid degradation. To elucidate the cellular proteolytic system involved in catab...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00668-7
更新日期:1994-08-01 00:00:00
abstract::Arabidopsis thaliana contains three genes with high homology to potato p24 which was described as a member of the Whirly family of nuclear transcriptional activators. Computer-based analysis revealed that all Arabidopsis Whirly (Why) proteins contain targeting sequences for either plastids or mitochondria. The functio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.05.059
更新日期:2005-07-04 00:00:00
abstract::The HBx protein of hepatitis B virus is involved in deregulation of cell cycle and development of hepatocellular carcinoma. Since c-Myc also plays an important role in cell proliferation and tumor development, we studied its regulation by HBx in a human hepatoma cell line. Co-expression of HBx and c-Myc resulted in in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.12.034
更新日期:2006-01-23 00:00:00
abstract::Dimethylsulphoxide (DMSO) reductase from R. capsulatus contains a molybdenum-pterin cofactor at its active site. As prepared the molybdenum is in the 6+ oxidation state, devoid of EPR signals. Stepwise reduction generates an EPR signal characteristic of Mo(V) having hyperfine coupling to a single proton and integratin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80760-e
更新日期:1992-07-28 00:00:00
abstract::The main immunogenic region (MIR) of the acetylcholine receptor (AChR) is the target for the majority of high-affinity autoantibodies produced in myasthenia gravis patients. Some monoclonal antibodies (mAbs) to the MIR bind specifically, but with low affinity, to synthetic AChR alpha subunit peptides with the sequence...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80286-r
更新日期:1990-07-02 00:00:00
abstract::Several recombinant proteins in inclusion bodies expressed in Escherichia coli have been measured by Fourier transform infrared and solid-state nuclear magnetic resonance spectra to provide the secondary structural characteristics of the proteins from hyperthermophilic archaeon Pyrococcus horikoshii OT3 (hyperthermoph...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01441-8
更新日期:2004-01-16 00:00:00
abstract::Type II restriction endonucleases (REases) exist in multiple oligomeric forms. The tetrameric REases have two DNA binding interfaces and must synapse two recognition sites to achieve cleavage. It was hypothesised that binding of two recognition sites by tetrameric enzymes contributes to their fidelity. Here, we experi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.07.009
更新日期:2012-09-21 00:00:00
abstract::The archaeal exosome, a protein complex responsible for phosphorolytic degradation and tailing of RNA, has an RNA-binding platform containing Rrp4, Csl4, and DnaG. Aiming to detect novel interaction partners of the exosome, we copurified Nop5, which is a part of an rRNA methylating ribonucleoprotein complex, with the ...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12915
更新日期:2017-12-01 00:00:00
abstract::It has previously been shown that jasmonic acid affects the ethylene signaling pathway. EIN2 is a central component of ethylene signaling that is downstream of the receptors. EIN2 has previously been shown to be required for ethylene responses. We found that reducing jasmonic acid levels, either mutationally or chemic...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.11.030
更新日期:2013-01-16 00:00:00
abstract::Using reverse transcription followed by PCR amplification (RT-PCR), we have identified multiple messenger RNAs encoding for the neuronal pore-forming Ca(2+) channel subunits alpha(1A) (P/Q channel), alpha(1B) (N channel), alpha(1D) (neuronal/endocrine L channel), alpha(1E) (R channel), alpha(1G-H) (T channel) and alph...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01984-0
更新日期:2000-09-22 00:00:00
abstract::In washed anterior pituitary membranes, there is enough GTP to occupy Ns and therefore to obtain activation of adenylate cyclase by vasointestinal peptide. GTP concentrations needed to obtain adenylate cyclase inhibition by dopamine (above 5 X 10- M) stimulate the adenylate cyclase. The dopamine effect is a blockade o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80741-8
更新日期:1983-09-05 00:00:00
abstract::The mammalian Ptdlns 3-kinase is shown to be inhibited by low nanomolar concentrations of demethoxyviridin, an antifungal agent structurally related to wortmannin. The inhibitory potency of both compounds could be observed in purified Ptdlns 3-kinase whether or not the regulatory subunit (p85 alpha) was present, sugge...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80482-6
更新日期:1994-04-04 00:00:00
abstract::VP64 is widely used as a transcriptional activator to investigate the biological function of genes, but its potential for application in genetic improvement of crops has not been fully investigated. Here, we characterized an OsMYB1R1-VP64 fusion protein that enhanced the grain yield of rice cultivar 'Kita-ake' by 35%....
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12374
更新日期:2016-10-01 00:00:00
abstract::Water-soluble amyloid beta-peptides (sA beta), ending at residue 42, precede amyloid plaques in Down's syndrome (DS). Here we report that sA beta consists of the full-length A beta(1-42) and peptides truncated and modified by cyclization of the N-terminal glutamates, A beta[3(pE)-42] and A beta[11(pE)-42]. The A beta[...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00564-4
更新日期:1997-06-16 00:00:00
abstract::Specific binding sites for 3,3'-T2 can be detected in swollen and osmotically treated mitochondria (OTM) from normal and hypothyroid rat liver. In hypothyroid animals, maximal values of binding were obtained at 0 degrees C while values were lower at 37 degrees C and no specific binding could be observed at 60 degrees ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00840-x
更新日期:1994-09-05 00:00:00
abstract::Molecular dynamics analyses were performed to examine conformational changes in the C-domain of calmodulin and the N-domain of troponin C induced by binding of Ca(2+) ions. Analyses of conformational changes in calmodulin and troponin C indicated that the shortening of the distance between Ca(2+) ions and Ca(2+) bindi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(04)00114-0
更新日期:2004-03-12 00:00:00
abstract::Tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF family of cytokines that promotes apoptosis and NF-kappaB activation. Here we show that recombinant hu-TRAIL initiates the activation of multiple caspases, the loss of mitochondrial transmembrane potential, the cleavage of BI...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01375-2
更新日期:2000-04-07 00:00:00
abstract::Phosphotyrosine-containing proteins in Dictyostelium discoideum were detected by immunoblot analysis and immunoprecipitation using a monoclonal anti-phosphotyrosine antibody. The iodinated antibody recognized on bots a cluster of 205-220 kDa polypeptides and bands of 107 and 60 kDa. The 107 and 60 kDa polypeptides and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81007-b
更新日期:1990-07-30 00:00:00
abstract::Mitochondrial translocation of ceramides triggers Bax-dependent apoptosis. To elucidate how ceramides activate Bax and commit cells to death, we developed a switchable version of the ceramide transfer protein CERT, sCERT. Upon its drug-induced recruitment to mitochondria, sCERT retains the ability to bind VAP proteins...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13956
更新日期:2020-11-01 00:00:00
abstract::Structural gene expression of human immunodeficiency virus type 1 (HIV-1) requires a viral regulatory protein, Rev transactivator. We investigated Rev-dependency of HIV-1 gene expression by various reporter systems. Expression of unspliced and single-spliced viral mRNAs was demonstrated to be differentially dependent ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00444-e
更新日期:1995-05-29 00:00:00
abstract::Abscisic acid (ABA) is a plant hormone involved in many developmental and physiological processes, but as yet, no ABA receptor has been identified. Flow cytometry of rice protoplasts and immunoblotting of purified plasma membranes (PMs) have been used to demonstrate that the monoclonal antibody JIM19 recognizes carboh...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00972-2
更新日期:1999-08-06 00:00:00
abstract::SufC is an ATPase component of the SUF machinery, which is involved in the biosynthesis of Fe-S clusters. To gain insight into the function of this protein, we have determined the crystal structure of Escherichia coli SufC at 2.5A resolution. Despite the similarity of the overall structure with ABC-ATPases (nucleotide...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.11.058
更新日期:2006-01-09 00:00:00
abstract::Previously, we cloned rat MRP3 as a candidate for an inducible transporter for the biliary excretion of organic anions [Hirohashi et al. (1998) Mol. Pharmacol. 53, 1068-10751. In the present study, we cloned human MRP3 (1527 amino acids) from Caco-2 cells. Human MRP3 is predominantly expressed in liver, small intestin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00899-0
更新日期:1998-08-14 00:00:00
abstract::In the present study we have examined the levels and phosphorylation state of the insulin receptor and insulin receptor substrate 1 (IRS-1) as well as the association between IRS-1 and phosphatidylinositol 3-kinase (PI 3-kinase) in the liver and muscle of rats treated with glucagon. There was a decrease in the insulin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00809-n
更新日期:1995-08-14 00:00:00
abstract::ADP-forming acetyl-CoA synthetase (ACD) catalyzes the interconversion of acetyl-CoA and acetate. The related succinyl-CoA synthetase follows a three-step mechanism involving a single phosphoenzyme, but a novel four-step mechanism with two phosphoenzyme intermediates was proposed for Pyrococcus ACD. Characterization of...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12573
更新日期:2017-02-01 00:00:00