Abstract:
:We have isolated four lambda-phages covering the complete pig aminopeptidase N/CD13 gene. The sequence of 2.85 kbp encompasses 1.18 kbp of the 5' upstream region and 1.67 kbp of the structural gene. In the promoter region we find a TATA box and potential binding sites for CTF-1/NF-1 and AP-2. By sequence comparisons we have found three domains showing similarity to promoter regions of the genes encoding human alpha 1-antitrypsin and human intestinal alkaline phosphatase. The gene sequence includes the first three exons and two introns. It shows that a single exon encodes the cytoplasmic tail, the membrane anchor and the junctional peptide.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Olsen J,Sjöström H,Norén Odoi
10.1016/0014-5793(89)81470-xsubject
Has Abstractpub_date
1989-07-17 00:00:00pages
275-81issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(89)81470-Xjournal_volume
251pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The hirudin-like factor 1 (HLF1) of Hirudo medicinalis belongs to a new class of leech-derived factors. In previous investigations, HLF1 did not exhibit anticoagulatory activities. Here, we describe the analysis of natural and synthetic variants of HLF1 and HLF-Hyb, a yet uncharacterized member of the HLF family. Modi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13683
更新日期:2020-03-01 00:00:00
abstract::Acute myeloid leukemia (AML) associated translocations often cause gene fusions that encode oncofusion proteins. Although many of the breakpoints involved in chromosomal translocations have been cloned, in most cases the role of the chimeric proteins in tumorigenesis is not elucidated. Here we will discuss the fusion ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.04.002
更新日期:2010-06-18 00:00:00
abstract::We present an open-access web platform isomiRex, to identify isomiRs and on the fly graphical visualization of the differentially expressed miRNAs in control as well as treated library. The open-access web-platform is not restricted only to NGS sequence dataset from animals and potentially analyzes a wider dataset for...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.06.047
更新日期:2013-08-19 00:00:00
abstract::We have purified a form of protein phosphatase 1 (PP1) from HeLa cell nuclei, in which the phosphatase is complexed to a regulatory subunit termed p99. We report here the cloning and characterisation of the p99 component. p99 mRNA is widely expressed in human tissues and immunofluorescence analysis with anti-p99 antib...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01485-3
更新日期:1997-12-22 00:00:00
abstract::Mechanisms underlying the association between fibroblastic growth factor 23 (FGF-23) and inflammation are uncertain. We found that FGF-23 was markedly up-regulated in LPS/INF-γ-induced proinflammatory M1 macrophages and Hyp mouse-derived peritoneal macrophages, but not in IL-4-induced M2 anti-inflammatory macrophages....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12040
更新日期:2016-01-01 00:00:00
abstract::The malaria parasite harbours an indispensable plastid known as the 'apicoplast'. The apicoplast's exact role remains uncertain, but it houses components involved in fatty acid, isoprenoid and haem biosyntheses. These pathways offer opportunities to develop anti-malarials. In the absence of photosynthesis, how apicopl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.11.097
更新日期:2010-02-05 00:00:00
abstract::The oxysterols, 7beta-hydroxycholesterol and 7-ketocholesterol, are involved in the cytotoxicity of oxidized LDL. To elucidate their molecular mechanisms, the human promonocytic leukemia cells U937 and U4 were used. U4 cells overexpressing Bcl-2 were obtained by transfection of U937 cells. 7Beta-hydroxycholesterol and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01473-7
更新日期:1997-12-15 00:00:00
abstract::The possible in vivo interaction of the Nicotiana tabacum agglutinin (Nictaba) with endogenous glycoproteins was corroborated using a combination of confocal/electron microscopy of an EGFP-Nictaba fusion protein expressed in tobacco Bright Yellow-2 (BY-2) cells and biochemical analyses. In vitro binding studies demons...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.10.044
更新日期:2006-11-27 00:00:00
abstract::The interaction of vinculin with actin filaments was investigated by methods which exclude interference by contaminating proteins which may occur in vinculin preparations. Vinculin which was blotted from SDS-polyacrylamide gels onto nitrocellulose, was stained specifically by fluorescently labeled polymeric actin (100...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80595-7
更新日期:1988-02-08 00:00:00
abstract::Constitutively active tyrosine kinases are frequently expressed in various types of human leukemias as the result of chromosomal translocations. The TEL-Jak2 fusion oncoprotein possesses transforming properties in both animal and cellular models, that are tightly dependent on Stat5 activation. In the IL-3-independent ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02452-8
更新日期:2001-05-25 00:00:00
abstract::CDK11(p58), a 58kDa protein of the PITSLRE kinase family, plays an important role in cell cycle progression, and is closely related to cell apoptosis. To gain further insight into the function of CDK11(p58), we screened a human fetal liver cDNA library for its interacting proteins using the yeast two-hybrid system. He...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.05.039
更新日期:2005-07-04 00:00:00
abstract::The bi-functional protein dimerization cofactor of HNF1 (DCoH)/pterin-4 alpha-carbinolamine dehydratase (PCD) is found in liver cell nuclei bound to the transcription factor hepatocyte nuclear factor 1 (HNF1) as well as in the cytoplasm acting as an enzyme involved in the phenylalanine hydroxylation system. Deficiency...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01325-u
更新日期:1995-01-02 00:00:00
abstract::Rat brain microtubules were prepared at the adult stage and from immature (i.e., 4-day-old) animals. At an early stage of development, the composition of microtubule-associated proteins is qualitatively different from that found at the adult stage [(1982) Eur. J. Biochem. 129, 465-471]. The influence of calmodulin on ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81148-5
更新日期:1984-07-09 00:00:00
abstract::A developmentally regulated exon has been identified in the 5'-alternatively spliced region of the human fast Troponin T (TnT) gene. Expressed in fetal (but not adult) muscle, this exon is homologous with the fetal exons recently described in the rabbit and rat fast TnT genes. They all exhibit a split codon organizati...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00729-2
更新日期:1994-08-15 00:00:00
abstract::A gene encoding an antifreeze protein (AFP) was isolated from carrot (Daucus carota) using sequence information derived from the purified protein. The carrot AFP is highly similar to the polygalacturonase inhibitor protein (PGIP) family of apoplastic plant leucine-rich repeat (LRR) proteins. Expression of the AFP gene...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00280-x
更新日期:1999-03-26 00:00:00
abstract::The kinetics of the tRNA cycle is in itself capable of keeping the translational error level almost unaffected by amino acid starvation. There is no need to assume any yet unknown mechanism or property. Kinetic analysis shows that the concentration of aminoacyl-tRNA can stay high even for large reductions in aminoacyl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80498-2
更新日期:1987-10-19 00:00:00
abstract::The addition of hepatocyte growth factor (HGF) to rat hepatocytes in primary culture resulted in the formation of inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) and 1,2-diacylglycerol (DG) by a phosphoinositide-specific phospholipase C (PI-PLC). DG showed a biphasic increase; the first phase, corresponding with the peak ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80554-t
更新日期:1992-02-10 00:00:00
abstract::The key mechanism in prion disease is the conversion of cellular prion protein into an altered, pathogenic conformation, in which cellular mechanisms play a poorly understood role. Both forms of prion protein are lipid-anchored and reside in rafts that appear to protect the native conformation against conversion. Neur...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2006.07.053
更新日期:2006-10-09 00:00:00
abstract::The Mahogany/Attractin gene (Atrn) has been proposed as a downstream mediator of Agouti signaling because yellow hair color and obesity in lethal yellow (A(y)) mice are suppressed by the mahogany (Atrn(mg)) mutation. The present study examined the distribution of Atrn mRNA in the brain and spinal cord by in situ hybri...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01494-5
更新日期:1999-11-26 00:00:00
abstract::Ceramide-1-phosphate (CER-1-P) is emerging as a novel bioactive sphingolipid. It is formed by phosphorylation of ceramide catalyzed by ceramide kinase, and has been implicated in different cellular processes. Cer-1-P is mitogenic for fibroblasts, blocks apoptosis in macrophages, controls phagocytosis in neutrophils, a...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(04)00211-x
更新日期:2004-03-26 00:00:00
abstract::Antibodies generated against synthetic peptides that correspond to highly conserved sequence motifs in the annexins reacted with a variety of annexins from different species. These include Xenopus laevis and Drosophila melanogaster, which contain cross-reacting polypeptides of apparent Mr 34,000 and 30,000. As expecte...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81668-0
更新日期:1989-12-04 00:00:00
abstract::A variant form of the heptacosapeptide amide secretin, with C-terminal -Val-Gly-Lys-Arg instead of valine amide, has been isolated from porcine upper intestinal tissue. Unexpectedly, this triacontapeptide exhibited a substantially higher bioactivity than the heptacosapeptide amide. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80636-0
更新日期:1985-05-20 00:00:00
abstract::Lectin-like oxidized low-density lipoprotein receptor (LOX-1) is a scavenger receptor that binds oxidized low-density lipoprotein (OxLDL) and has a role in atherosclerosis development. The N-terminus intracellular region (cytoplasmic domain) of LOX-1 mediates receptor internalization and trafficking, potentially throu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.04.049
更新日期:2014-06-13 00:00:00
abstract::Malonyl-CoA-acyl carrier protein transacylase (MCAT) transfers the malonyl group from malonyl-CoA to holo-acyl carrier protein (ACP), and since malonyl-ACP is a key building block for fatty-acid biosynthesis it is considered as a promising antibacterial target. The crystal structures of MCAT from Staphylococcus aureus...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.02.038
更新日期:2010-03-19 00:00:00
abstract::A one-electron reductive metabolism of 1,2-dibromoethane (DBE) is described that gives rise to a free radical intermediate, which can be stabilized by a spin trapping agent and detected by electron spin resonance spectroscopy. Using rat liver microsomes or isolated hepatocytes from phenobarbitone pretreated animals, u...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80964-8
更新日期:1983-08-22 00:00:00
abstract::Acetylcholinesterase (AChE) is likely to have roles other than the hydrolysis of acetylcholine, e.g., related to developmental processes like neurite outgrowth, differentiation and adhesion. Here, we investigated whether AChE can function as a heterophilic cell adhesion molecule and searched for proteins interacting w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.08.078
更新日期:2004-10-08 00:00:00
abstract::Mg2+ -dependent catechol-O-methyltransferases occur in animals as well as in bacteria, fungi and plants, often with a pronounced selectivity towards one of the substrate's hydroxyl groups. Here, we show that the bacterial MxSafC exhibits excellent regioselectivity for para as well as for meta methylation, depending on...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12530
更新日期:2017-01-01 00:00:00
abstract::Extracts of the alkaliphilic sulfur-oxidizing autotroph strain AL3 contained sulfide:cytochrome c oxidoreductase. This was active above pH 8, and was associated with the cell membranes. Although up to 60% of the initial activity was lost during Triton X-100 extraction, further purification resulted in an enzyme that c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00379-2
更新日期:1998-05-01 00:00:00
abstract::We present evidence that the potent chloride channel blocker NPPB has protonophoric activity in the mitochondria and across the plasma membrane of phagocytic cells. The resting O2 consumption of murine peritoneal macrophages was stimulated up to 2.5-fold in the presence of NPPB, with a K0.5 of 15 microM. The stimulato...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80992-c
更新日期:1991-08-19 00:00:00
abstract::Apicomplexan parasites contain a vestigial plastid called apicoplast which has been suggested to be a site of [Fe-S] cluster biogenesis. Here we report the cloning of lipoic acid synthase (LipA) from Toxoplasma gondii, a well known [Fe-S] protein. It is able to complement a LipA-deficient Escherichia coli strain, clea...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00673-2
更新日期:2003-07-17 00:00:00