isomiRex: web-based identification of microRNAs, isomiR variations and differential expression using next-generation sequencing datasets.

abstract：:The complete amino acid sequence of the oligomycin sensitivity-conferring protein (OSCP) of beef-heart mitochondria is reported. The protein contains 190 amino acids and has a molecular mass of 20 967. Its structure is characterized by a concentration of charged amino acids in the two terminal segments (N 1-77 and C 1...

journal_title：FEBS letters

authors： Ovchinnikov YA,Modyanov NN,Grinkevich VA,Aldanova NA,Trubetskaya OE,Nazimov IV,Hundal T,Ernster L

更新日期：1984-01-23 00:00:00

abstract：:Mitochondrial translocation of ceramides triggers Bax-dependent apoptosis. To elucidate how ceramides activate Bax and commit cells to death, we developed a switchable version of the ceramide transfer protein CERT, sCERT. Upon its drug-induced recruitment to mitochondria, sCERT retains the ability to bind VAP proteins...

journal_title：FEBS letters

authors： Jain A,Dadsena S,Holthuis JCM

更新日期：2020-11-01 00:00:00

abstract：:The Kv3.1 channel plays a crucial role in regulating the high-frequency firing properties of neurons. Here, we determined whether Src regulates the subcellular distributions of the Kv3.1b channel. Co-expression of active Src induced a dramatic redistribution of Kv3.1b to the endoplasmic reticulum. Furthermore, co-expr...

journal_title：FEBS letters

authors： Bae SH,Kim DH,Shin SK,Choi JS,Park KS

更新日期：2014-01-03 00:00:00

abstract：:We have measured SGLT1 mRNA content and SGLT1-mediated glucose transport at different positions along the small intestine of control and streptozotocin diabetic rats and shown both parameters to be similar but higher in jejunal compared with ileal tissue. No such correlation was seen when comparing measurements of SGL...

journal_title：FEBS letters

authors： Dong R,Srai SK,Debnam E,Smith M

更新日期：1997-04-07 00:00:00

abstract：:Stability of green fluorescent protein (GFP) is sometimes important for a proper practical application of this protein. Random mutagenesis and targeted mutagenesis have been used to create better-folded variants of GFP, including recently reported extra-superfolder GFP. Our aim was to determine the crystal structure o...

journal_title：FEBS letters

authors： Choi JY,Jang TH,Park HH

更新日期：2017-01-01 00:00:00

abstract：:Tissue-transglutaminase (t-TGase) is a family of calcium-dependent enzymes. A Ca2+-independent soluble enzyme, in addition to t-TGase, capable of incorporating polyamines into proteins was demonstrated in rat intestinal mucosa. The Ca2+-independent enzyme was stimulated 2- to 5-fold by Fe2+ and Co2+ ions but inhibited...

journal_title：FEBS letters

authors： Tsai YH,Lai WF,Wu YW,Johnson LR

更新日期：1998-09-18 00:00:00

abstract：:The crystal structure for cce_0566 (171 aa, 19.4 kDa), a DUF269 annotated protein from the diazotrophic cyanobacterium Cyanothece sp. ATCC 51142, was determined to 1.60Å resolution. Cce_0566 is a homodimer with each molecule composed of eight α-helices folded on one side of a three strand anti-parallel β-sheet. Hydrop...

journal_title：FEBS letters

authors： Buchko GW,Robinson H

更新日期：2012-02-17 00:00:00

abstract：:Random sequences of 120-130 amino acid residues were inserted into a surface loop region of Escherichia coli RNase HI. This library was screened and about 10% of the clones were found to retain RNase H activity. Subsequent random mutagenesis led to an increase in RNase H activity and solubility of the protein. The ins...

journal_title：FEBS letters

authors： Doi N,Itaya M,Yomo T,Tokura S,Yanagawa H

更新日期：1997-02-03 00:00:00

abstract：:Yeast Golgi ecto-ATPase Ynd1p is an unusual type III membrane protein with the longest translocated N-terminus reported. Sequential deletion analysis reveals that translocation of this 500-residue-long hydrophilic domain across the membranes requires the C-terminal transmembrane domain of Ynd1p and its flanking region...

journal_title：FEBS letters

authors： Zhong X,Malhotra R,Guidotti G

更新日期：2005-10-24 00:00:00

abstract：:The fraction of polyclonal caldesmon antibodies cross-reacting with rabbit skeletal troponin T are shown to compete with smooth muscle tropomyosin for caldesmon and troponin T, as revealed by ELISA method. The epitope recognized by these antibodies was also found in Mr 77 kDa non-muscle caldesmon. These results provid...

journal_title：FEBS letters

authors： Birukov KG,Shirinsky VP,Vorotnikov AV,Gusev NB

更新日期：1990-03-26 00:00:00

abstract：:The hypothesis that calpain subunits dissociate in the presence of Ca2+ has been tested by methods which avoid interference by Ca2+-induced aggregation and large subunit autolysis. Inactive Cys105Ser-m-calpain, bound either to Ni-NTA-agarose or to immobilized casein, after incubation with Ca2+, could be recovered in h...

journal_title：FEBS letters

authors： Dutt P,Arthur JS,Croall DE,Elce JS

更新日期：1998-10-09 00:00:00

abstract：:Ballistic transfection, based on cell and tissue bombardment by the tungsten and gold microparticles covered with the gene DNA, was used for the delivery of a bacterial beta-galactosidase and a full-length cDNA copy of the human dystrophin genes into mouse skeletal muscles. CMV-lacZ, SV40-lacZ, LTR-lacZneo and full-le...

journal_title：FEBS letters

authors： Zelenin AV,Kolesnikov VA,Tarasenko OA,Shafei RA,Zelenina IA,Mikhailov VV,Semenova ML,Kovalenko DV,Artemyeva OV,Ivaschenko TE,Evgrafov OV,Dickson G,Baranovand VS

更新日期：1997-09-08 00:00:00

abstract：:A bovine anterior pituitary polypeptide that stimulates the replication of rat and human adipocyte precursors has been purified. Its Mr is 44 000-53 000 and its isoelectric point is 9.8-10.3. While pituitary basic fibroblast growth factor is equally mitogenic on adipocyte precursors and skin fibroblasts, the polypepti...

journal_title：FEBS letters

authors： Lau DC,Roncari DA,Yip DK,Kindler S,Nilsen SG

更新日期：1983-03-21 00:00:00

abstract：:The hyperthermophilic archaeon Sulfolobus solfataricus metabolises glucose and galactose by a 'promiscuous' non-phosphorylative variant of the Entner-Doudoroff pathway, in which a series of enzymes have sufficient substrate promiscuity to permit the metabolism of both sugars. Recently, it has been proposed that the pa...

journal_title：FEBS letters

authors： Lamble HJ,Theodossis A,Milburn CC,Taylor GL,Bull SD,Hough DW,Danson MJ

更新日期：2005-12-19 00:00:00

abstract：:We have used a panel of monoclonal antibodies in a study of the expression of multiple tubulins in Physarum polycephalum. Three anti-beta-tubulin monoclonal antibodies, DM1B, DM3B3 and KMX-1 all reacted with the beta 1-tubulin isotypes expressed in both myxamoebae and plasmodia. However, these antibodies showed a spec...

journal_title：FEBS letters

authors： Birkett CR,Foster KE,Johnson L,Gull K

更新日期：1985-08-05 00:00:00

abstract：:Transgenic Escherichia coli expressing pyrroloquinoline-quinone (PQQ) synthase gene from Deinococcus radiodurans showed superior survival during Rose Bengal induced oxidative stress. Such cells showed significantly low levels of protein carbonylation as compared to non-transgenic control. In vitro, PQQ reacted with re...

journal_title：FEBS letters

authors： Misra HS,Khairnar NP,Barik A,Indira Priyadarsini K,Mohan H,Apte SK

更新日期：2004-12-03 00:00:00

abstract：:The omega3 fatty-acid desaturases: FAD7 and FAD8 (plastid) and FAD3 (reticular) are responsible for trienoic fatty-acid (TA) production in plants. The expression of these enzymes seemed to be regulated differently in response to light. Darkness leads to a decrease in total TA level. Under such conditions, FAD3 and FAD...

journal_title：FEBS letters

authors： Collados R,Andreu V,Picorel R,Alfonso M

更新日期：2006-09-04 00:00:00

abstract：:Dried cells of Brevibacterium ammoniagenes are a good enzyme source for the preparative synthesis of CoA from pantothenic acid, L-cysteine and ATP. A problem with this synthesis is that the CoA synthesis is repressed by negative feedback inhibition by CoA to pantothenate kinase, the first step enzyme for the biosynthe...

journal_title：FEBS letters

authors： Shimizu S,Komaki R,Tani Y,Yamada H

更新日期：1983-01-24 00:00:00

abstract：:Mutations in the presenilin (PS) genes PSI and PS2 are involved in Alzheimer's disease (AD). Recently, apoptosis-associated cleavage of PS proteins was identified. Here we demonstrate that PS1 as well as PS2 are substrates for different members of the caspase protein family. Remarkably, the caspases acting on PS1 coul...

journal_title：FEBS letters

authors： van de Craen M,de Jonghe C,van den Brande I,Declercq W,van Gassen G,van Criekinge W,Vanderhoeven I,Fiers W,van Broeckhoven C,Hendriks L,Vandenabeele P

更新日期：1999-02-19 00:00:00

abstract：:We identified the d-galacturonic acid (GA)-responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger, which was found to be essential for growth on GA and polygalacturonic acid (PGA). Growth of the ΔgaaR strain was reduced on complex pectins. Genome-wide expression analysis showed that Ga...

journal_title：FEBS letters

authors： Alazi E,Niu J,Kowalczyk JE,Peng M,Aguilar Pontes MV,van Kan JA,Visser J,de Vries RP,Ram AF

更新日期：2016-06-01 00:00:00

abstract：:Epigenetic silencing mechanisms play an important role in chemoresistance of human cancer. Here we report the upregulated expression of metastasis-associated protein 1 (MTA1), a component of the nucleosome remodeling deacetylation (NuRD) complex, in chemoresistant prostate cancer (PCa). MTA1 knockdown in PC-3 cells in...

journal_title：FEBS letters

authors： Yu L,Su YS,Zhao J,Wang H,Li W

更新日期：2013-08-19 00:00:00

abstract：:Prefibrillar cytotoxicity was suggested as a common amyloid characteristic. We showed two types of albebetin prefibrillar oligomers are formed during incubation at pH 7.3. Initial round-shaped oligomers consist of 10-15 molecules determined by atomic force microscopy, do not bind thioflavin-T and do not affect viabili...

journal_title：FEBS letters

authors： Zamotin V,Gharibyan A,Gibanova NV,Lavrikova MA,Dolgikh DA,Kirpichnikov MP,Kostanyan IA,Morozova-Roche LA

更新日期：2006-05-01 00:00:00

abstract：:Calcium ionophore (A23187)-induced high molecular weight (HMW) and internucleosomal DNA fragmentation were investigated in human leukemia cell lines. An apoptosis-sensitive cell line, HL-60, showed HMW, internucleosomal DNA fragmentation and morphological changes of apoptosis by A23187. MOLT-4, which is resistant to a...

journal_title：FEBS letters

authors： Kataoka A,Kubota M,Wakazono Y,Okuda A,Bessho R,Lin YW,Usami I,Akiyama Y,Furusho K

更新日期：1995-05-15 00:00:00

abstract：:Cytotoxic effects were observed following 4 h incubation of human leukaemic cells with the iron chelator 1-hydroxypyridine-2-thione (omadine). Its cytotoxic activity was comparable to that of the cytotoxic drug doxorubicin. At the same concentration two other effective iron chelators, desferrioxamine and 1,2-dimethyl-...

journal_title：FEBS letters

authors： Kontoghiorghes GJ,Piga A,Hoffbrand AV

更新日期：1986-08-18 00:00:00

abstract：:Two tandem cassettes, one containing the telomerase reverse transcriptase gene (hTERT) promoter upstream of a constitutively activated form of heat shock transcription factor 1 (cHSF1) and followed by the other containing the heat shock protein 70B (hsp70B) promoter (HSE) upstream of the cytosine deaminase (CD) gene, ...

journal_title：FEBS letters

authors： Wang J,Yao M,Zhang Z,Gu J,Zhang Y,Li B,Sun L,Liu X

更新日期：2003-07-10 00:00:00

abstract：:Incorporation of dioleoyl N-(monomethoxy polyethyleneglycol succinyl)phosphatidylethanolamine (PEG-PE) into large unilamellar liposomes composed of egg phosphatidylcholine:cholesterol (1:1) does not significantly increase the content leakage when the liposomes are exposed to 90% human serum at 37 degrees C, yet the li...

journal_title：FEBS letters

authors： Klibanov AL,Maruyama K,Torchilin VP,Huang L

更新日期：1990-07-30 00:00:00

abstract：:During the course of large scale purification of the D1 dopamine receptor from rat brain, a protein of approximately 87,000 daltons (p87) was observed to copurify with the D1 receptor through four chromatographic steps. To characterize the nature of this protein, bovine and rat cDNA clones were isolated and sequenced....

journal_title：FEBS letters

authors： Gingrich JA,Andersen PH,Tiberi M,el Mestikawy S,Jorgensen PN,Fremeau RT Jr,Caron MG

更新日期：1992-11-09 00:00:00

abstract：:The genes for the hypothalamic hormones vasopressin and oxytocin are located in close proximity to each other within the rat genome. They are separated by only approx. 11 kbp of DNA sequence and oriented in such a way that their transcription occurs on opposite DNA strands. Although the two genes are structurally very...

journal_title：FEBS letters

authors： Mohr E,Bahnsen U,Kiessling C,Richter D

更新日期：1988-12-19 00:00:00

abstract：:We have expressed in E. coli segments of the rod portion of rabbit skeletal fast muscle myosin and compared physical properties of two different species, LMM-30 and LMM-30C'. LMM-30 consists of 263 amino acids including the original C-terminus of myosin heavy chain. LMM-30C' is colinear with LMM-30, but is devoid of 1...

journal_title：FEBS letters

authors： Maeda K,Rösch A,Maéda Y,Kalbitzer HR,Wittinghofer A

更新日期：1991-04-09 00:00:00

abstract：:Activation of T-cells infected by HIV-1 results in activation of long terminal repeat (LTR)-dependent viral transcription and ultimately the production of infectious virus. Although full T-cell activation requires a complex series of intracellular signals, including protein kinase C activation, calcium mobilisation, a...

journal_title：FEBS letters

authors： Baldari CT,Macchia G,Massone A,Telford JL

更新日期：1992-06-15 00:00:00