Abstract:
:Calcium ionophore (A23187)-induced high molecular weight (HMW) and internucleosomal DNA fragmentation were investigated in human leukemia cell lines. An apoptosis-sensitive cell line, HL-60, showed HMW, internucleosomal DNA fragmentation and morphological changes of apoptosis by A23187. MOLT-4, which is resistant to apoptosis, exhibited only HMW DNA fragmentation and died of necrosis under the same conditions. Autodigestion experiments suggested the endonucleolytic activity to cause HMW fragmentation in the cytoplasm of both cell lines. The activity was more dependent on Mg2+ than Ca2+ in HL-60, whereas it was Ca(2+)-dependent in MOLT-4. These results suggest that HMW DNA fragmentation is not specific to apoptosis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kataoka A,Kubota M,Wakazono Y,Okuda A,Bessho R,Lin YW,Usami I,Akiyama Y,Furusho Kdoi
10.1016/0014-5793(95)00405-xsubject
Has Abstractpub_date
1995-05-15 00:00:00pages
264-7issue
3eissn
0014-5793issn
1873-3468pii
001457939500405Xjournal_volume
364pub_type
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