Enhanced suicide gene therapy by chimeric tumor-specific promoter based on HSF1 transcriptional regulation.

abstract：:Mediatophore is a protein purified from the nerve terminal membranes of Torpedo electric organ. It confers to artificial membranes a calcium-dependent mechanism that translocates acetylcholine. When similar reconstitution experiments are applied to rat brain synaptosomal membranes they reveal the presence of mediatoph...

journal_title：FEBS letters

authors： Israël M,Lesbats B,Morel N,Manaranche R,Le Gal la Salle G

更新日期：1988-06-20 00:00:00

abstract：:The pH dependence of spin-spin coupling constants J (P-O-P) of ADP and ATP has been determined from the phosphorus NMR spectra at 0 degrees. The variation curves have been interpreted as titration curves. These give different pK values. Comparison with the tripolyphosphate shows the existence of triphosphate chain str...

journal_title：FEBS letters

authors： Ellenberger M,Brehamet L,Villemin M,Toma F

更新日期：1970-06-01 00:00:00

abstract：:The effects of guanine nucleotides on arachidonic acid (AA) release were studied in intact and saponin-permeabilized human platelets. While GTP[S] itself caused a stimulation of AA release in permeabilized cells, GTP[S], GDP[S], GTP, ATP and other nucleotides inhibited AA release in response to thrombin and other agon...

journal_title：FEBS letters

authors： Rehm AG,Wu H,Halenda SP

更新日期：1988-07-18 00:00:00

abstract：:The ATPase Cdc48 is required for membrane fusion and protein degradation. Recently it has been suggested that Cdc48 in a complex with Ufd1 and Npl4 acts as an ubiquitin-dependent chaperone. Here it is shown that recombinant Cdc48 alone can distinguish between the native and the non-native conformation of model substra...

journal_title：FEBS letters

authors： Thoms S

更新日期：2002-06-05 00:00:00

abstract：:We describe the use of a phage-displayed random pentadecamer peptide library for searching glycosphingolipid mimicking peptides. Two phage clones (AD-1 and AD-2) were selected by biopanning using monoclonal antibody AD117m, directed to lactotetraosylceramide (Lc4Cer). The amino acid sequences of the selected clones sh...

journal_title：FEBS letters

authors： Taki T,Ishikawa D,Hamasaki H,Handa S

更新日期：1997-11-24 00:00:00

abstract：:The human DNA-dependent activator of IFN-regulatory factor (DAI) protein, which activates the innate immune response in response to DNA, contains two tandem Z-DNA binding domains (Zα and Zβ) at the NH(2) terminus. The hZβ(DAI) structure is similar to other Z-DNA binding proteins, although it demonstrates an unusual Z-...

journal_title：FEBS letters

authors： Kim HE,Ahn HC,Lee YM,Lee EH,Seo YJ,Kim YG,Kim KK,Choi BS,Lee JH

更新日期：2011-03-09 00:00:00

abstract：:The Xenopus laevis oocyte has endogenous sites that bind muscarinic agonists, which have been pharmacologically characterized as M3 and/or M1 receptor subtypes. In order to define the molecular identify of the receptor protein we have analyzed a Xenopus oocyte cDNA library and cloned a 2.9 kb cDNA fragment encoding a ...

journal_title：FEBS letters

authors： Herrera L,Carvallo P,Antonelli M,Olate J

更新日期：1994-09-26 00:00:00

abstract：:The protein kinase C activator, phorbol-12-myristate-13-acetate (PMA), augments the cyclic AMP accumulation induced by forskolin in pheochromocytoma (PC 12) cells with an EC50 value of 14 nM, while having no effect on basal values. At a concentration of 100 nM PMA markedly augmented the magnitude of the forskolin resp...

journal_title：FEBS letters

authors： Hollingsworth EB,Ukena D,Daly JW

更新日期：1986-02-03 00:00:00

abstract：:Oligonucleotide probes based on the known amino acid sequence of salmon calcitonin were used to screen a cDNA library obtained from ultimobranchial glands of salmon for clones encoding salmon calcitonin. From the cDNA sequence of strongly hybridizing clones the complete primary structure of the calcitonin precursor co...

journal_title：FEBS letters

authors： Pöschl E,Lindley I,Hofer E,Seifert JM,Brunowsky W,Besemer J

更新日期：1987-12-21 00:00:00

abstract：:A conformational transition between E2 and E1 forms of Na, K-ATPase induced by different nucleotides has been studied under steady state conditions using the enzyme labelled with 5-iodoacetamidofluorescein. In the presence of K+ the plot of fluorescence as a function of [ATP], [ADP] or [CTP] (in a range of 5 microM-12...

journal_title：FEBS letters

authors： Lopina OD,Pindel EV,Boldyrev AA

更新日期：1990-06-18 00:00:00

abstract：:VacA is a major virulence factor of the widespread stomach-dwelling bacterium Helicobacter pylori. It causes cell vacuolation and tissue damage by forming anion-selective, urea-permeable channels in plasma and endosomal membranes. We report that several flavone derivatives and other polyphenols present in vegetables a...

journal_title：FEBS letters

authors： Tombola F,Campello S,De Luca L,Ruggiero P,Del Giudice G,Papini E,Zoratti M

更新日期：2003-05-22 00:00:00

abstract：:Amiloride, a specific inhibitor for the Na(+)-driven flagellar motors of alkalophilic Bacillus, is known to inhibit secondarily the growth of alkalophiles. The motility of a marine Vibrio, V. alginolyticus, was almost completely inhibited by 2 mM amiloride either at pH 7.0 or 8.5. We found that this concentration of a...

journal_title：FEBS letters

authors： Atsumi T,Maekawa Y,Tokuda H,Imae Y

更新日期：1992-12-14 00:00:00

abstract：:A novel member of the neurotrophin family, zebrafish neurotrophin-7 (zNT-7), was isolated from the zebrafish Danio rerio. The amino acid sequence of zNT-7 is more closely related to that of fish nerve growth factor (NGF) and neurotrophin-6 (NT-6) than to that of any other neurotrophin. zNT-7 is, however, equally relat...

journal_title：FEBS letters

authors： Nilsson AS,Fainzilber M,Falck P,Ibáñez CF

更新日期：1998-03-13 00:00:00

abstract：:Initiation of translation in hepatitis C virus (HCV) is dependent on the presence of an internal ribosome entry site (IRES) contained in its 341-nt-long 5'-untranslated region (UTR). This region is very conserved among different isolates and has been used to classify HCV isolates in six different genotypes. These geno...

journal_title：FEBS letters

authors： Buratti E,Gerotto M,Pontisso P,Alberti A,Tisminetzky SG,Baralle FE

更新日期：1997-07-14 00:00:00

abstract：:Plasmid pGC20 containing the (dGC)9 insert in SmaI recognition site has been used to study the inhibition of cleavage by different restriction endonuclease due to Z-DNA formation in (dCG)10 sequence of the negatively supercoiled plasmid. Data obtained indicate the different sensitivity of restriction endonucleases to ...

journal_title：FEBS letters

authors： Lesnik EA,Bes'chetnikova ZhA,Maslova RN,Varshavsky JaM

更新日期：1991-03-11 00:00:00

abstract：:The nature of the G-proteins present in the pre- and post-synaptic plasma membranes and in the synaptic vesicles of cholinergic nerve terminals purified from the Torpedo electric organ was investigated. In pre- and post-synaptic plasma membranes, Bordetella pertussis toxin, known to catalyze the ADP-ribosylation of th...

journal_title：FEBS letters

authors： Toutant M,Bockaert J,Homburger V,Rouot B

更新日期：1987-09-28 00:00:00

abstract：:The membrane-membrane fusion during fertilization of oocyte by spermatozoa is believed to be mainly mediated by so called "fusion proteins". In the present study we have tried to demonstrate that beside the proteins, lipid components of membrane may play an important role in fusion of oocyte with spermatozoa. Conventi...

journal_title：FEBS letters

authors： Atif SM,Hasan I,Ahmad N,Khan U,Owais M

更新日期：2006-04-17 00:00:00

abstract：:The GAL11 gene encodes an auxiliary transcription factor required for full expression of many, if not all, genes of the yeast Saccharomyces cerevisiae. We have recently shown that GAL11-encoded protein (Gal11p) enhances basal transcription from the CYC1 promoter in a cell-free transcription system [(1993) Proc. Natl. ...

journal_title：FEBS letters

authors： Sakurai H,Ohishi T,Amakasu H,Fukasawa T

更新日期：1994-09-05 00:00:00

abstract：:CXCR4 (fusin) is a chemokine receptor which is involved as a coreceptor in gp120 binding to the cell surface. In this study we provide evidence that binding of gp120 triggers CXCR4 recruitment to glycosphingolipid-enriched microdomains. Scanning confocal microscopy showed a nearly complete localization of CXCR4 within...

journal_title：FEBS letters

authors： Sorice M,Garofalo T,Misasi R,Longo A,Mattei V,Sale P,Dolo V,Gradini R,Pavan A

更新日期：2001-09-28 00:00:00

abstract：:Respiration in all cells depends upon synthesis of ATP by the ATP synthase complex, a rotary motor enzyme. The structure of the catalytic moiety of ATP synthase, the so-called F(1) headpiece, is well established. F(1) is connected to the membrane-bound and ion translocating F(0) subcomplex by a central stalk. A periph...

journal_title：FEBS letters

authors： Dudkina NV,Heinemeyer J,Keegstra W,Boekema EJ,Braun HP

更新日期：2005-10-24 00:00:00

abstract：:The yeast gene, ACP1, encoding the mitochondrial acyl carrier protein, was deleted by gene replacement. The resulting acp1-deficient mutants had only 5-10% of the wild-type lipoic acid content remaining, and exhibited a respiratory-deficient phenotype. Upon meiosis, the lipoate deficiency co-segregated with the acp1 d...

journal_title：FEBS letters

authors： Brody S,Oh C,Hoja U,Schweizer E

更新日期：1997-05-19 00:00:00

abstract：:The synthesis of gangliosides GM3 and GD3 is carried out by the successive addition of sialic acid residues on lactosylceramide (LacCer) by the Golgi located sialyltransferases Sial-T1 and Sial-T2, respectively. CHO-K1 cells lack Sial-T2 and only express GM3. Here we show that the activity of Sial-T1 was near 2.5-fold...

journal_title：FEBS letters

authors： Spessott W,Crespo PM,Daniotti JL,Maccioni HJ

更新日期：2012-07-30 00:00:00

abstract：:Mouse embryonic stem (ES) cells homozygous for disruption of the MSK1 gene had no detectable MSK1 activity. However, their activators (extracellular signal related kinase (ERK)1/ERK2) were stimulated normally in mitogen- and stress-activated protein kinase (MSK)1-/- and wild type cells in response to tetradecanoylphor...

journal_title：FEBS letters

authors： Arthur JS,Cohen P

更新日期：2000-09-29 00:00:00

abstract：:In the methylerythritol phosphate pathway for isoprenoid biosynthesis, the GcpE/IspG enzyme catalyzes the conversion of 2-C-methyl-d-erythritol 2,4-cyclodiphosphate into (E)-4-hydroxy-3-methylbut-2-enyl diphosphate. This reaction requires a double one-electron transfer involving a [4Fe-4S] cluster. A thylakoid prepara...

journal_title：FEBS letters

authors： Seemann M,Tse Sum Bui B,Wolff M,Miginiac-Maslow M,Rohmer M

更新日期：2006-03-06 00:00:00

abstract：:The integrin alpha IIb beta 3-mediated redistribution of the tyrosine kinases pp125FAK and pp60Src and the small GTP-binding proteins CDC42Hs and Rap1B from the membrane skeleton to the cytoskeleton was found to be reversible: upon prolonged platelet aggregation (up to 15 min) induced by the thrombin-receptor activati...

journal_title：FEBS letters

authors： Dash D,Aepfelbacher M,Siess W

更新日期：1995-04-24 00:00:00

abstract：:We have investigated the effect of botulinum neurotoxin (BoNT) C1 light chain (LC) on insulin exocytosis from the clonal beta-cell line HIT-T15. In streptolysin-O permeabilized cells, the beta-cell impermeant BoNT C1 cleaved mainly syntaxin 1 and inhibited Ca2+ as well as GTPgammaS induced exocytosis. To study the eff...

journal_title：FEBS letters

authors： Land J,Zhang H,Vaidyanathan VV,Sadoul K,Niemann H,Wollheim CB

更新日期：1997-12-08 00:00:00

abstract：:Oncostatin M (OSM) is a potent modulator of human lung-derived epithelial cell function. This cytokine binds two distinct receptor complexes: type I OSM receptor which is also a functional receptor for leukemia inhibitory factor (LIF), and type II OSM-specific receptor. The role of these two distinct receptors in medi...

journal_title：FEBS letters

authors： Cichy J,Rose-John S,Puré E

更新日期：1998-06-16 00:00:00

abstract：:An experimental procedure is described for the reproducible production of hybridoma cells using the electrofusion technique. High yields can be obtained when fusion is performed in isotonic inositol solutions containing Ca2+ and Mg2+ in a ratio of 1:5 in the millimolar range. The hybridoma cells are transferred 10 min...

journal_title：FEBS letters

authors： Vienken J,Zimmermann U

更新日期：1985-03-25 00:00:00

abstract：:A novel antimicrobial peptide, named misgurin, was isolated and characterized from the loach (mudfish), Misgurnus anguillicaudatus. The 21-amino-acid peptide with a molecular mass of 2502 Da was purified to homogeneity using a heparin-affinity column and C18 reverse-phase and gel-permeation high-performance liquid chr...

journal_title：FEBS letters

authors： Park CB,Lee JH,Park IY,Kim MS,Kim SC

更新日期：1997-07-14 00:00:00

abstract：:The effect of persistent measles virus infection on c-fos protooncogene and protein kinase C (PKC-I) gene expression in a murine neuroblastoma cell line was studied. Overexpression of c-fos protooncogene by infected NS20Y/MS cells was detected when compared with uninfected NS20Y cells. The level of PKC-I-specific mRNA...

journal_title：FEBS letters

authors： Wolfson M,Katorza A,Segal S,Rager-Zisman B

更新日期：1989-07-03 00:00:00