Expression of the vasopressin and oxytocin genes in rats occurs in mutually exclusive sets of hypothalamic neurons.


:The genes for the hypothalamic hormones vasopressin and oxytocin are located in close proximity to each other within the rat genome. They are separated by only approx. 11 kbp of DNA sequence and oriented in such a way that their transcription occurs on opposite DNA strands. Although the two genes are structurally very similar including common potential regulatory elements in their putative promotor regions, they are expressed in discrete populations of magnocellular neurons of the hypothalamus. In rats placed under osmotic stress, the vasopressin gene is upregulated; concomitantly transcription of the oxytocin gene is also stimulated. To address the question of whether this coordinated rise in oxytocin-encoding mRNA is the result of switching on oxytocin gene transcription in vasopressinergic neurons, in situ hybridization with double labelled cRNA probes was carried out. Biotinylated and [alpha-35S]CTP labelled antisense cRNA probes specific for either vasopressin or oxytocin mRNA were constructed and hybridized to hypothalamic sections from salt-loaded rats. The results demonstrate that upregulation of oxytocin gene transcription is restricted solely to oxytocinergic cells; no oxytocin gene transcripts can be detected in vasopressinergic neurons.




FEBS letters


Mohr E,Bahnsen U,Kiessling C,Richter D




Has Abstract


1988-12-19 00:00:00














  • Evidence that nebulin is a protein-ruler in muscle thin filaments.

    abstract::Partial amino acid sequence was obtained from the massive myofibrillar protein nebulin. This consists of repeating motifs of about 35 residues and super-repeats of 7 x 35 = 245 residues. The repeat-motifs are likely to be largely alpha-helical and to interact with both actin and tropomyosin in thin filaments. Nebulin ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Labeit S,Gibson T,Lakey A,Leonard K,Zeviani M,Knight P,Wardale J,Trinick J

    更新日期:1991-05-06 00:00:00

  • The structural elements of hirudin which bind to the fibrinogen recognition site of thrombin are exclusively located within its acidic C-terminal tail.

    abstract::Six lysyl residues of human thrombin (LysB21, LysB52, LysB65, LysB106, LysB107 and LysB154) have been previously shown to participate in the binding site of hirudin, a thrombin-specific inhibitor [(1989) J. Biol. Chem. 264, 7141-7146]. In this report, we attempted to delineate the region of hirudin which binds to thes...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chang JY,Ngai PK,Rink H,Dennis S,Schlaeppi JM

    更新日期:1990-02-26 00:00:00

  • Occurrence of bifurcated three-center hydrogen bonds in proteins.

    abstract::Analysis of 13 high-resolution protein X-ray crystal structures shows that 1204 (24%) of all the 4974 hydrogen bonds are of the bifurcated three-center type with the donor X-H opposing two acceptors A1, A2. They occur systematically in alpha-helices where 90% of the hydrogen bonds are of this type; the major component...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Preissner R,Egner U,Saenger W

    更新日期:1991-08-19 00:00:00

  • In vitro translation of cytosolic and peroxisomal epoxide hydrolase and catalase on liver polyribosomes from untreated and clofibrate-treated C57B1/6 mice.

    abstract::Free and membrane-bound polyribosomes were isolated from the livers of untreated and clofibrate-treated male C57B1/6 mice. The in vitro translation products were investigated in a rabbit reticulocyte cell-free system by immunoprecipitation of cytosolic epoxide hydrolase, catalase and albumin. The soluble forms of epox...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Joste V,Meijer J

    更新日期:1989-05-22 00:00:00

  • Insulin stimulates the translocation of protein kinase C in rat adipocytes.

    abstract::Insulin-induced changes in protein kinase C were examined in cytosol and membrane fractions of rat adipocytes enzymatically after Mono Q column chromatography and by immunoblotting. During a 5-20 min period of insulin treatment, cytosolic protein kinase C decrease by approximately 50%, whereas membrane protein kinase ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ishizuka T,Cooper DR,Farese RV

    更新日期:1989-11-06 00:00:00

  • Inhibition of Na,K-ATPase activity by cGMP is isoform-specific in brain endothelial cells.

    abstract::cGMP has been shown to either activate or inhibit Na,K-ATPase activity. Using mouse brain endothelial cells which express both ouabain-resistant alpha1 and ouabain-sensitive alpha2 and alpha3 isoforms, we show that cGMP reduces total Na,KATPase activity to about 58%. The inhibition is prevented by the protein kinase G...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pontiggia L,Winterhalter K,Gloor SM

    更新日期:1998-10-09 00:00:00

  • Analysis of U1snRNP-specific A protein cross-linked complexes.

    abstract::The organization of the U1snRNP-specific A protein (34 kDa) has been analyzed by 12 and 16 A thiol-reversible chemical cross-linking and Western blotting. A-containing cross-linked complexes had molecular masses of 43, 47, 56, 62, 67, 105 and 125 kDa. None of these complexes could be cross-linked following ribonucleas...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Boix J,Habets WJ,van Venrooij WJ,Smith HC

    更新日期:1990-10-15 00:00:00

  • Expression of c-fos gene inhibits proteoglycan synthesis in transfected chondrocyte.

    abstract::The effect of expression of c-fos gene on proteoglycan synthesis, one of the important markers of cartilage metabolism, was examined by introducing the c-fos DNA into HCS 2/8 chondrocytes. The [35S]sulfate incorporation into proteoglycan was decreased in the c-fos transfectants expressing exogenous c-fos mRNA, when co...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tsuji M,Funahashi S,Takigawa M,Seiki M,Fujii K,Yoshida T

    更新日期:1996-03-04 00:00:00

  • Molecular cloning and sequence analysis of cDNA encoding human cholesterol 7 alpha-hydroxylase.

    abstract::A complete cDNA clone encoding human cholesterol 7 alpha-hydroxylase has been isolated using a rat P-450ch7 alpha cDNA insert [(1989) FEBS Lett. 257, 97-100] as a probe and totally sequenced. The cDNA contained 1512-base pair open reading frame encoding 504 amino acid residues (Mr 57,630), 39-base pair 5'-untranslated...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Noshiro M,Okuda K

    更新日期:1990-07-30 00:00:00

  • Negative regulation of the anti-human immunodeficiency virus and chemotactic activity of human stromal cell-derived factor 1alpha by CD26/dipeptidyl peptidase IV.

    abstract::Stromal cell-derived factor 1alpha (SDF-1alpha) is a chemokine that has been shown to prevent infection of T-tropic HIV strains and is a possible substrate of CD26/dipeptidyl peptidase IV (DPPIV). In this study, we show that SDF-1alpha was cleaved at the N-terminal region by CD26/DPPIV and as a result the inhibitory a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ohtsuki T,Hosono O,Kobayashi H,Munakata Y,Souta A,Shioda T,Morimoto C

    更新日期:1998-07-17 00:00:00

  • Enhanced cellular uptake of biotinylated antisense oligonucleotide or peptide mediated by avidin, a cationic protein.

    abstract::The cellular uptake of a model antisense oligonucleotide complementary to 21 bases of the bovine GLUT-1 glucose transporter mRNA and a model vasopressin peptide that were biotinylated, was markedly stimulated by the presence of avidin, a cationic protein. Conversely, the bacteria homologue of avidin, streptavidin, whi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pardridge WM,Boado RJ

    更新日期:1991-08-19 00:00:00

  • H2O2 activates Nox4 through PLA2-dependent arachidonic acid production in adult cardiac fibroblasts.

    abstract::Stimulated production of reactive oxygen species (ROS) by plasma membrane-associated nicotinamide adenine dinucleotide phosphate oxidases (Nox) in non-phagocytic cells regulates a number of biological processes including growth, vessel tone, and oxygen sensing. The purpose of this study was to investigate H(2)O(2)-sti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Colston JT,de la Rosa SD,Strader JR,Anderson MA,Freeman GL

    更新日期:2005-04-25 00:00:00

  • Determination of amino acids critical to the main immunogenic region of intact acetylcholine receptors by in vitro mutagenesis.

    abstract::The main immunogenic region (MIR) of the acetylcholine receptor (AChR) is the target for the majority of high-affinity autoantibodies produced in myasthenia gravis patients. Some monoclonal antibodies (mAbs) to the MIR bind specifically, but with low affinity, to synthetic AChR alpha subunit peptides with the sequence...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Saedi MS,Anand R,Conroy WG,Lindstrom J

    更新日期:1990-07-02 00:00:00

  • Selective inhibition of the mitochondrial permeability transition pore at the oxidation-reduction sensitive dithiol by monobromobimane.

    abstract::In this paper we introduce monobromobimane, a thiol reagent, as a selective blocker of the recently identified dithiol whose oxidation-reduction status modifies voltage sensing by the mitochondrial permeability transition pore, a cyclosporin A-sensitive channel. Monobromobimane does not inhibit the phosphate carrier, ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Costantini P,Chernyak BV,Petronilli V,Bernardi P

    更新日期:1995-04-03 00:00:00

  • The human 32-kDa stress protein induced by exposure to arsenite and cadmium ions is heme oxygenase.

    abstract::Exposure of HeLa and HL60 cells to sodium arsenite or cadmium chloride led to marked increases in cellular heme oxygenase activity. SDS-polyacrylamide gel electrophoresis of [35S]methionine-labeled cellular proteins indicated that these treatments also resulted in the induction of a 32-kDa protein. Immunoblot analysis...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Taketani S,Kohno H,Yoshinaga T,Tokunaga R

    更新日期:1989-03-13 00:00:00

  • YDL142c encodes cardiolipin synthase (Cls1p) and is non-essential for aerobic growth of Saccharomyces cerevisiae.

    abstract::The unassigned open reading frame YDL142c was identified to code for cardiolipin synthase, Cls1p. A cls1 deletion strain is viable on glucose, galactose, ethanol, glycerol and lactate containing media, although the growth rate on non-fermentable carbon sources is decreased. Mitochondria of the cls1 mutant are devoid o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tuller G,Hrastnik C,Achleitner G,Schiefthaler U,Klein F,Daum G

    更新日期:1998-01-02 00:00:00

  • Expression of Mona (monocytic adapter) in myeloid progenitor cells results in increased and prolonged MAP kinase activation upon macrophage colony-stimulating factor stimulation.

    abstract::Mona is an SH3 and SH2 domain-containing adapter molecule that is induced during monocytic differentiation. Here we have first shown that M-CSFR is the major Mona partner in M-CSF signaling, the interaction being mediated through tyrosine 697 of the receptor. Next we asked whether Mona expression would alter the Ras/M...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bourgin C,Bourette R,Mouchiroud G,Arnaud S

    更新日期:2000-09-01 00:00:00

  • Crystallization and crystallographic investigations of the small subunit of mouse ribonucleotide reductase.

    abstract::The R2 protein component of mouse ribonucleotide reductase has been obtained from overproducing Escherichia coli bacteria. It has been crystallized using NaCl as precipitant. The crystals are orthorhombic, space group C222(1), with cell dimensions a = 76.9 A, b = 108.9 A, c = 92.7 A and diffract to at least 2.5 A. The...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nielsen BB,Kauppi B,Thelander M,Thelander L,Larsen IK,Eklund H

    更新日期:1995-10-16 00:00:00

  • Does ferredoxin I (Azotobacter) represent a novel class of DNA-binding proteins that regulate gene expression in response to cellular iron(II)?

    abstract::Azotobacter vinelandii (Av) and chroococcum (Ac) ferredoxin I contain [3Fe-4S]1 + 0 and [4Fe-4S]2+1+ clusters, when isolated aerobically, which undergo one-electron redox cycles at potentials of -460 +/- 10 mV (vs SHE) at pH 8.3 and -645 +/- 10 mV, respectively. The X-ray structure of Fd I (Av) reveals that the N-term...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Thomson AJ

    更新日期:1991-07-22 00:00:00

  • Adiponectin receptor binding proteins--recent advances in elucidating adiponectin signalling pathways.

    abstract::Adiponectin whose systemic levels are reduced in obesity-related diseases ameliorates insulin sensitivity and regulates biological processes like apoptosis, proliferation, migration and inflammation. Adiponectin binds to adiponectin receptors, AdipoR1 and AdipoR2, which are ubiquitously expressed. Clathrin-dependent e...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Buechler C,Wanninger J,Neumeier M

    更新日期:2010-10-22 00:00:00

  • Studies on the interaction of the dye, stains-all, with individual calcium-binding domains of calmodulin.

    abstract::We show that the calcium-mimic dye, Stains-all, is a convenient probe to study the structural features of the individual calcium-binding sites of calmodulin (CaM) and related calcium-binding proteins (CaBP). These peptides bind the dye in their calcium-binding sites, and induce a circular dichroism (CD) band in the bo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sharma Y,Gopalakrishna A,Balasubramanian D,Fairwell T,Krishna G

    更新日期:1993-07-12 00:00:00

  • Autocatalytic processes in cooperative mechanisms of prion diseases.

    abstract::According to the leading theory, the agent responsible for prion diseases would be the conformational isomer PrP(Sc) of a cellular protein PrP(C), the pathogenic form PrP(Sc) multiplying by converting the normal protein into a likeness of itself. The pathogenic isoform could catalyze the conformational transition so t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Laurent M

    更新日期:1997-04-21 00:00:00

  • MicroRNA-206 regulates surfactant secretion by targeting VAMP-2.

    abstract::Lung surfactant secretion is a highly regulated process. Our previous studies have shown that VAMP-2 is essential for surfactant secretion. In the present study we investigated the role of miR-206 in surfactant secretion through VAMP-2. VAMP-2 was confirmed to be a target of miR-206 by 3'-untranslational region (3'-UT...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zhang H,Guo Y,Mishra A,Gou D,Chintagari NR,Liu L

    更新日期:2015-01-02 00:00:00

  • MFH-1 is required for bone morphogenetic protein-2-induced osteoblastic differentiation of C2C12 myoblasts.

    abstract::Mesenchyme forkhead-1 (MFH-1), a winged helix/forkhead transcription factor, is expressed in developing cartilaginous tissues, kidney and arch arteries, and is essential for the normal development of the axial skeleton and aortic arch formation of mice. To investigate the possible role of MFH-1 in osteogenesis and ost...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yang XL,Matsuura H,Fu Y,Sugiyama T,Miura N

    更新日期:2000-03-17 00:00:00

  • Sargaquinoic acid and sargahydroquinoic acid from Sargassum yezoense stimulate adipocyte differentiation through PPARalpha/gamma activation in 3T3-L1 cells.

    abstract::We screened active compounds from natural marine products able to increase PPARalpha/gamma transcriptional activity. Sargaquinoic acid (SQA) and sargahydroquinoic acid (SHQA) from Sargassum yezoense were identified as novel PPARalpha/gamma dual agonists. The binding affinity of SQA with PPARgamma was higher than that ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kim SN,Choi HY,Lee W,Park GM,Shin WS,Kim YK

    更新日期:2008-10-15 00:00:00

  • The maximum of the histone acetyltransferase activity precedes DNA-synthesis in regenerating rat liver.

    abstract::A pronounced transitory increase of histone acetyltransferase (EC activity before the onset of DNA replication is shown to occur in regenerating rat liver. This effect is followed by an increase in the H1/H1(0) ratio at the start of DNA synthesis; H1(0) de is replaced by H1. Histone acetylation and H1/H1(0) ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Weiss G,Puschendorf B

    更新日期:1988-09-26 00:00:00

  • X-linked inhibitor of apoptosis protein mediates neddylation by itself but does not function as a NEDD8-E3 ligase for caspase-7.

    abstract::X-linked inhibitor of apoptosis protein (XIAP) is a potent antagonist of caspases, and functions as a ubiquitin-E3 ligase by itself and for caspases. Recently, NEDD8, a ubiquitin-like modifier, has been suggested to be used for modification of caspase-7 mediated by XIAP. However, it is not clear whether caspase-7 is a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nagano T,Hashimoto T,Nakashima A,Kikkawa U,Kamada S

    更新日期:2012-06-04 00:00:00

  • Metabolic engineering to produce phytochromes with phytochromobilin, phycocyanobilin, or phycoerythrobilin chromophore in Escherichia coli.

    abstract::By co-expression of heme oxygenase and various bilin reductase(s) in a single operon in conjunction with apophytochrome using two compatible plasmids, we developed a system to produce phytochromes with various chromophores in Escherichia coli. Through the selection of different bilin reductases, apophytochromes were a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mukougawa K,Kanamoto H,Kobayashi T,Yokota A,Kohchi T

    更新日期:2006-02-20 00:00:00

  • Lipopolysaccharide signaling induces serum amyloid A (SAA) synthesis in human hepatocytes in vitro.

    abstract::To investigate the role of lipopolysaccharide (LPS) in hepatocyte activation, we examined the expression of Toll-like receptor 4 (TLR4), the putative receptor for LPS in human hepatocytes. TLR4 mRNA and protein expression was confirmed in human hepatocytes. Stimulation of human hepatocytes with LPS results in rapid de...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Migita K,Abiru S,Nakamura M,Komori A,Yoshida Y,Yokoyama T,Daikoku M,Ueki T,Takii Y,Yano K,Yastuhashi H,Eguchi K,Ishibashi H

    更新日期:2004-07-02 00:00:00

  • Conformational change of 50 S ribosomes on enzymatic binding of phenylalanyl-tRNA.

    abstract::Tight couple 70 S ribosomes are converted to loose couple ones on enzymatic binding of phenylalanyl-tRNA. Enzymatic binding at 0 degree C as well as nonenzymatic binding does not lead to any change. Further, no change takes place when the P site is occupied by N-acetylphenylalanyl-tRNA. Loose couple 70 S ribosomes are...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Srivastava S,Burma DP

    更新日期:1985-07-01 00:00:00