Abstract:
:Transgenic Escherichia coli expressing pyrroloquinoline-quinone (PQQ) synthase gene from Deinococcus radiodurans showed superior survival during Rose Bengal induced oxidative stress. Such cells showed significantly low levels of protein carbonylation as compared to non-transgenic control. In vitro, PQQ reacted with reactive oxygen species with rate constants comparable to other well known antioxidants, producing non-reactive molecular products. PQQ also protected plasmid DNA and proteins from the oxidative damage caused by gamma-irradiation in solution. The data suggest that radioprotective/oxidative stress protective ability of PQQ in bacteria may be consequent to scavenging of reactive oxygen species per se and induction of other free radical scavenging mechanism.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Misra HS,Khairnar NP,Barik A,Indira Priyadarsini K,Mohan H,Apte SKdoi
10.1016/j.febslet.2004.10.061keywords:
subject
Has Abstractpub_date
2004-12-03 00:00:00pages
26-30issue
1-2eissn
0014-5793issn
1873-3468pii
S0014579304013158journal_volume
578pub_type
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