A constitutive form of heat-shock protein 70 is located in the outer membranes of mitochondria from rat liver.


:HSP73, the constitutive form of heat-shock protein 70, has been implicated in the translocation of preproteins across the mitochondrial membranes, being required for maintaining mitochondrial preproteins in an import competent conformation. Here we report that highly purified mitochondrial outer membranes contain a protein indistinguishable from HSP73 as a tightly associated peripheral component of the membrane. This membrane form of HSP73 was photolabelled with [alpha-32P]ATP and could be released from the outer membrane with sodium carbonate, but not after incubation of the membranes with salt or with ATP. A sensitive immunoassay with an anti-HSP73 monoclonal antibody, revealed the association of HSP73 with mitochondrial outer membrane vesicles at a level similar to that of preprotein import receptors.




FEBS letters


Lithgow T,Ryan M,Anderson RL,Høj PB,Hoogenraad NJ




Has Abstract


1993-10-18 00:00:00














  • Ca2+-modulated ONE-GC odorant signal transduction.

    abstract::In a subset of olfactory epithelium the odorant receptor guanylate cyclase, ONE-GC, is a central transduction component of the cyclic GMP signaling pathway. The odorant binds to the extracellular domain and activates its intracellular catalytic domain to generate the odorant second messenger, cyclic GMP. The present s...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Duda T,Sharma RK

    更新日期:2009-04-17 00:00:00

  • Selective inhibition of the mitochondrial permeability transition pore at the oxidation-reduction sensitive dithiol by monobromobimane.

    abstract::In this paper we introduce monobromobimane, a thiol reagent, as a selective blocker of the recently identified dithiol whose oxidation-reduction status modifies voltage sensing by the mitochondrial permeability transition pore, a cyclosporin A-sensitive channel. Monobromobimane does not inhibit the phosphate carrier, ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Costantini P,Chernyak BV,Petronilli V,Bernardi P

    更新日期:1995-04-03 00:00:00

  • Homing markers for atherosclerosis: applications for drug delivery, gene delivery and vascular imaging.

    abstract::Endothelial dysfunction plays a major role in the pathogenesis of atherosclerosis. Pro-inflammatory cytokines such as interleukin-1 beta and tumour necrosis factor alpha activate endothelial cells changing their resting phenotype to become pro-adhesive, pro-thrombotic and pro-atherogenic. Phage display in vivo biopann...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Houston P,Goodman J,Lewis A,Campbell CJ,Braddock M

    更新日期:2001-03-09 00:00:00

  • Evidence for sialidase hydrolyzing gangliosides GM2 and GM1 in rat liver plasma membrane.

    abstract::Rat liver plasma membrane removed sialic acid from mixed bovine brain gangliosides more efficiently than from sialyllactose and orosomucoid with an optimal pH of 4.5. When individual gangliosides, each labeled with [14C]sialic acid or [3H]sphingosine, were tested, not only GD1a and GM3 but also GM2 and GM1, both of wh...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Miyagi T,Tsuiki S

    更新日期:1986-10-06 00:00:00

  • Involvement of a 65 kDa phosphoprotein in the regulation of membrane fusion during exocytosis in Paramecium cells.

    abstract::Antisera were raised against a phosphoprotein of 65 kDa (PP65) from Paramecium cells (shown before to be selectively dephosphorylated during synchronous exocytosis) and specified by immunoblotting. By immunofluorescence PP65 has been localized within the cortex, beneath the cell membrane. This corresponds to data obta...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Stecher B,Höhne B,Gras U,Momayezi M,Glas-Albrecht R,Plattner H

    更新日期:1987-10-19 00:00:00

  • The effect of ammonia and pH on brain gamma-glutamyl transpeptidase in young rats.

    abstract::Acute hyperammonemia, induced by two consecutive injections of ammonium acetate (550 and 450 mg per kg b.wt.), decreased the activity of gamma-glutamyl transpeptidase (GGT) in most brain regions of 18- and 30-day-old rats. This decrease in the brain GGT activity was more pronounced in younger than in older rats. After...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Stastný F,Pitha J,Lisý V,Hilgier W,Kaucká I,Albrecht J

    更新日期:1992-04-06 00:00:00

  • The primary structure of the glutamic acid-specific protease of Streptomyces griseus.

    abstract::The amino acid sequence and part of the DNA sequence of a glutamic acid-specific serine protease from Streptomyces griseus is reported. This protease is shown to be homologous with other serine proteases. An improved purification protocol for this enzyme is described. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Svendsen I,Jensen MR,Breddam K

    更新日期:1991-11-04 00:00:00

  • The 49-kDa subunit of NADH-ubiquinone oxidoreductase (Complex I) is involved in the binding of piericidin and rotenone, two quinone-related inhibitors.

    abstract::Piericidin is a potent inhibitor of the mitochondrial and bacterial type I NADH-ubiquinone oxidoreductases (Complex I) and is considered to bind at or close to the ubiquinone binding site(s) of the enzyme. Piericidin-resistant mutants of the bacterium Rhodobacter capsulatus have been isolated and the present work demo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Darrouzet E,Issartel JP,Lunardi J,Dupuis A

    更新日期:1998-07-10 00:00:00

  • Substrate recognition by casein kinase-II: the role of histidine-160.

    abstract::Casein kinase-II (CK-II) belongs to the protein kinases recognizing serine/threonine in proximity to acidic residues in protein substrates. Crystallography and mutagenesis studies on the cAMP-dependent protein kinase (PKA) disclosed that glutamic acid-170 (E170), is important for interaction of substrates with the enz...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dobrowolska G,Meggio F,Marin O,Lozeman FJ,Li D,Pinna LA,Krebs EG

    更新日期:1994-12-05 00:00:00

  • Red and far-red light alter the transcript profile in the cyanobacterium Synechocystis sp. PCC 6803: impact of cyanobacterial phytochromes.

    abstract::Cyanobacteria possess genes encoding phytochrome-related proteins. We used a DNA microarray approach to evaluate the impact of the phytochromes Cph1 and Cph2 on red light (R)- and far-red light (FR)-dependent gene expression in the unicellular cyanobacterium Synechocystis sp. PCC 6803. In cells of wild-type and phytoc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hübschmann T,Yamamoto H,Gieler T,Murata N,Börner T

    更新日期:2005-03-14 00:00:00

  • Interaction of 10Sa RNA with ribosomes in Escherichia coli.

    abstract::10Sa RNA is a bacterial small stable RNA, in which the 5'- and 3'-end sequences are folded into a tRNA-like structure. The RNA accepts alanine in vitro, and interacts with 70S ribosomes in the cells. In this study, we examined the ribosome-binding properties of Escherichia coli 10Sa RNA in vivo, and found that the ami...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tadaki T,Fukushima M,Ushida C,Himeno H,Muto A

    更新日期:1996-12-16 00:00:00

  • The G protein-coupled receptor rhodopsin in the native membrane.

    abstract::The higher-order structure of G protein-coupled receptors (GPCRs) in membranes may involve dimerization and formation of even larger oligomeric complexes. Here, we have investigated the organization of the prototypical GPCR rhodopsin in its native membrane by electron and atomic force microscopy (AFM). Disc membranes ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fotiadis D,Liang Y,Filipek S,Saperstein DA,Engel A,Palczewski K

    更新日期:2004-04-30 00:00:00

  • The chloride channel blocker 5-nitro-2-(3-phenylpropyl-amino) benzoic acid (NPPB) uncouples mitochondria and increases the proton permeability of the plasma membrane in phagocytic cells.

    abstract::We present evidence that the potent chloride channel blocker NPPB has protonophoric activity in the mitochondria and across the plasma membrane of phagocytic cells. The resting O2 consumption of murine peritoneal macrophages was stimulated up to 2.5-fold in the presence of NPPB, with a K0.5 of 15 microM. The stimulato...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lukacs GL,Nanda A,Rotstein OD,Grinstein S

    更新日期:1991-08-19 00:00:00

  • Temperature effects on the presteady-state and transport-associated currents of GABA cotransporter rGAT1.

    abstract::The effects of temperature on the gamma-aminobutyric acid (GABA) uptake and on the presteady-state and transport-associated currents of the GABA cotransporter, rat gamma-aminobutyric acid transporter 1 (rGAT1), have been studied using heterologous oocyte expression and voltage-clamp. Increasing temperature from 15 to ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Binda F,Bossi E,Giovannardi S,Forlani G,Peres A

    更新日期:2002-02-13 00:00:00

  • Chemical synthesis and biological activity of a novel antibacterial peptide deduced from a pig myeloid cDNA.

    abstract::Several myeloid precursors of antibacterial peptides have recently been shown to share homologous pre- and pro-regions. Taking advantage of this homology, a novel cDNA was cloned from pig bone marrow RNA. This encodes a 166-residue polypeptide with highly conserved pre- (29 residues) and pro- (101 residues) sequences,...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Storici P,Scocchi M,Tossi A,Gennaro R,Zanetti M

    更新日期:1994-01-17 00:00:00

  • Receptor-activated cytoplasmic Ca2+ spikes in communicating clusters of pancreatic acinar cells.

    abstract::The transmission of receptor-generated cytoplasmic Ca2+ signals between communicating pancreatic acinar cells has been investigated by comparing patch-clamp recordings of Ca(2+)-dependent Cl- current in internally perfused single cells and small multi-cell clusters. Acetylcholine (50 nM) generates shortlasting repetit...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Petersen CC,Petersen OH

    更新日期:1991-06-17 00:00:00

  • Mammalian ER stress sensor IRE1β specifically down-regulates the synthesis of secretory pathway proteins.

    abstract::Accumulation of unfolded proteins in the endoplasmic reticulum (ER) causes ER stress. The ER stress sensor inositol requiring enzyme-1beta (IRE1β), which is specifically expressed in intestinal epithelial cells, is thought to be involved in translational repression. However, its mechanism of action is not fully unders...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nakamura D,Tsuru A,Ikegami K,Imagawa Y,Fujimoto N,Kohno K

    更新日期:2011-01-03 00:00:00

  • Cloning and functional analysis of the hematopoietic cell-specific phospholipase C(gamma)2 promoter.

    abstract::Phospholipase C(gamma)2 (PLCgamma2) is a phospholipid-converting enzyme which, upon receptor stimulation, is activated within membrane-bound signalling complexes. In contrast to the highly ubiquitous PLCgamma1, PLCgamma2 is expressed predominantly in B-lymphocytes. Associated with antigen-coupling receptors it is acti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kang JS,Kohlhuber F,Hug H,Marmé D,Eick D,Ueffing M

    更新日期:1996-12-09 00:00:00

  • Crystallization and crystallographic investigations of the small subunit of mouse ribonucleotide reductase.

    abstract::The R2 protein component of mouse ribonucleotide reductase has been obtained from overproducing Escherichia coli bacteria. It has been crystallized using NaCl as precipitant. The crystals are orthorhombic, space group C222(1), with cell dimensions a = 76.9 A, b = 108.9 A, c = 92.7 A and diffract to at least 2.5 A. The...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nielsen BB,Kauppi B,Thelander M,Thelander L,Larsen IK,Eklund H

    更新日期:1995-10-16 00:00:00

  • Diffusion of fluorescein-labelled molecules in suspensions of erythrocyte ghosts.

    abstract::The diffusion of fluorescein isothiocyanate-labelled dextran molecules in suspensions of centrifugally, tightly packed, erythrocyte ghosts was measured by fluorescence recovery after photobleaching. In comparison with diffusion in aqueous solution, the diffusion coefficients for probe molecules of varying size were ab...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Järnefelt J,Laurent T,Rigler R

    更新日期:1988-12-19 00:00:00

  • TAK1 mediates an activation signal from toll-like receptor(s) to nuclear factor-kappaB in lipopolysaccharide-stimulated macrophages.

    abstract::Stimulation of monocytes/macrophages with lipopolysaccharide (LPS) results in activation of nuclear factor-kappaB (NF-kappaB), which plays crucial roles in regulating expression of many genes involved in the subsequent inflammatory responses. Here, we investigated roles of transforming growth factor-beta activated kin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Irie T,Muta T,Takeshige K

    更新日期:2000-02-11 00:00:00

  • Phosphorylation of protein kinase C by casein kinase-1.

    abstract::Because phosphorylation of protein kinase C (PKC) may provide a mechanism for regulation of this enzyme, we have examined the ability of two other kinases to phosphorylate PKC. Our results show that casein kinase 1 (CK-1), but not casein kinase 2 (CK-2), can phosphorylate PKC in the absence of Ca2+ and phospholipids. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vila J,Walker JM,Itarte E,Weber MJ,Sando JJ

    更新日期:1989-09-11 00:00:00

  • The binding of the fluorescent ATP analogue 2'(3')-trinitrophenyladenosine-5'-triphosphate to rat liver fatty acid-binding protein.

    abstract::The less polar fluorescent analogue of ATP, 2'(3')-trinitrophenyl-5'-triphosphate bound to rat liver fatty acid-binding protein with high affinity (Kd 6.3 x 10(-6) M) and 1:1 molar stoichiometry. This probe bound to the fatty acid binding site of the protein and was displaced by oleic acid and oleoyl CoA. High concent...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sheridan M,Wilton DC

    更新日期:1992-12-21 00:00:00

  • Selective inhibition of lignoceroyl-CoA synthetase by adenosine 5'-alkylphosphates.

    abstract::Structural analogs of adenosine 5'-acylphosphates, which are intermediates of the reaction catalyzed by acyl-CoA synthetases, were synthesized by condensing primary alcohols with AMP to examine the inhibitory effects on the lignoceroyl-CoA and palmitoyl-CoA synthetase activities. Hexadecyl, octadecyl, eicosyl, docosyl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Shiraishi T,Tezuka K,Uda Y

    更新日期:1994-10-03 00:00:00

  • Conventional protein kinase C isoforms regulate human dopamine transporter activity in Xenopus oocytes.

    abstract::The hypothesis that specific protein kinase C (PKC) isoforms regulate dopamine transporter (DAT) function was tested in Xenopus laevis oocytes expressing human (h)DAT. Activation of conventional PKCs (cPKCs) and novel PKCs (nPKCs) using 10 nM phorbol 12-myristate 13-acetate (PMA) significantly inhibited DAT-associated...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Doolen S,Zahniser NR

    更新日期:2002-04-10 00:00:00

  • Cold-sensitive phenotypes of a yeast null mutant of ARV1 support its role as a GPI flippase.

    abstract::Glycosylphosphatidylinositol (GPI) is synthesized in the endoplasmic reticulum (ER) and added onto proteins to form GPI-anchored proteins. Among the many proteins involved in this process, ACAT-related enzyme-2 required for viability 1 (Arv1) is a candidate, functioning as a flippase that translocates GPI intermediate...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Okai H,Ikema R,Nakamura H,Kato M,Araki M,Mizuno A,Ikeda A,Renbaum P,Segel R,Funato K

    更新日期:2020-08-01 00:00:00

  • Synthesis of holo Paracoccus denitrificans cytochrome c550 requires targeting to the periplasm whereas that of holo Hydrogenobacter thermophilus cytochrome c552 does not. Implications for c-type cytochrome biogenesis.

    abstract::Expression from a plasmid of the complete gene, including the codons for the N-terminal periplasmic targeting signal, for cytochrome c550 of Paracoccus denitrificans led to the formation of the holo protein in the periplasms of both P. denitrificans and Escherichia coli. Expression of the gene from which the region co...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sambongi Y,Ferguson SJ

    更新日期:1994-02-28 00:00:00

  • Stabilization of a compact conformation of monomeric GroEL at low temperature by adenine nucleotides.

    abstract::E. coli GroEL chaperonin monomers, isolated after urea-induced dissociation of GroEL14, undergo cold denaturation below 5 degrees C. Above 5 degrees C, these monomers undergo MgATP-dependent self-assembly. We have demonstrated a conformational transition at 0 degree C induced by interaction of monomeric GroEL with ade...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lissin NM,Hemmingsen SM

    更新日期:1993-06-07 00:00:00

  • Alternative splicing of the human Shaker K+ channel beta 1 gene and functional expression of the beta 2 gene product.

    abstract::Mammalian voltage-activated Shaker K+ channels associate with at least three cytoplasmic proteins: Kv beta 1, Kv beta 2 and Kv beta 3. These beta subunits contain variable N-termini, which can modulate the inactivation of Shaker alpha subunits, but are homologous throughout an aldo-keto reductase core. Human and ferre...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: McCormack K,McCormack T,Tanouye M,Rudy B,Stühmer W

    更新日期:1995-08-14 00:00:00

  • On the role of N-7-mercaptoheptanoyl-O-phospho-L-threonine (component B) in the enzymatic reduction of methyl-coenzyme M to methane.

    abstract::The reduction of methyl-coenzyme M (CH3SCoM) to methane in methanogenic bacteria is dependent on component B (N-7-mercaptoheptanoyl-O-phospho-L-threonine, HSHTP). We report here that S-methyl-component B (N-7-(methylthio)heptanoyl-O-phospho-L-threonine, CH3SHTP) can substitute for neither CH3SCoM nor HSHTP in the meth...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ellermann J,Kobelt A,Pfaltz A,Thauer RK

    更新日期:1987-08-17 00:00:00