A constitutive form of heat-shock protein 70 is located in the outer membranes of mitochondria from rat liver.

abstract：:A complete cDNA encoding rabbit uteroglobin was constructed and expressed in MDCK and Caco-2 cells. The MDCK cells secrete uteroglobin in approximately equal amounts to the apical and the basolateral side, whereas the Caco-2 cells secrete uteroglobin mainly to the basolateral side. Both MDCK and Caco-2 cells thus secr...

journal_title：FEBS letters

authors： Vogel LK,Suske G,Beato M,Norén O,Sjöström H

更新日期：1993-09-20 00:00:00

abstract：:KOR-3, also known as ORL-1, is a member of the opioid receptor family, encoding the murine receptor for orphanin FQ/nociceptin. In the current studies we have identified five different splice variants of KOR-3 in mouse brain, three of which have not been previously reported. In addition to variants with a 15 bp deleti...

journal_title：FEBS letters

authors： Pan YX,Xu J,Wan BL,Zuckerman A,Pasternak GW

更新日期：1998-09-11 00:00:00

abstract：:The retinal pigment epithelium-specific 65 kDa (RPE65) isomerase plays a pivotal role in photoreceptor survival and function. RPE65-catalyzed synthesis of 11-cis-retinol from all-trans-retinyl esters in the visual cycle is negatively regulated, through a heretofore unknown mechanism, by the fatty acid transport protei...

journal_title：FEBS letters

authors： Li S,Green JF,Jin M

更新日期：2020-02-01 00:00:00

abstract：:Excessive reactive oxygen species (ROS) play a key role in the pathogenesis of diabetic nephropathy. The thioredoxin (TRX) system, a major thiol antioxidant system, regulates the reduction of intracellular ROS. Here we show that high glucose (HG) inhibits TRX ROS-scavenging function through p38 mitogen-activated prote...

journal_title：FEBS letters

authors： Ren Y,Shi Y,Wang Y,Li Y,Wu S,Li H,Zhang Y,Duan H

更新日期：2010-08-04 00:00:00

abstract：:A hybrid protein from Neisseria meningitidis, which contains both a peroxiredoxin and a glutaredoxin domain, has been isolated. The enzyme was active in the reduction of various peroxides and dehydroascorbate in the presence of reduced glutathione. These findings suggest that both the peroxiredoxin and glutaredoxin do...

journal_title：FEBS letters

authors： Rouhier N,Jacquot JP

更新日期：2003-11-06 00:00:00

abstract：:Serine/threonine phosphatases such as PP1, PP2A, and PP2B are well known to regulate the transition phase of the cell cycle. However, the function of PP2Cgamma in cell cycle progression is still unclear. In the present study, we report the characterization of PP2Cgamma in mammalian cells during the cell cycle. After r...

journal_title：FEBS letters

authors： Suh EJ,Kim TY,Kim SH

更新日期：2006-11-13 00:00:00

abstract：:VinJ is an amidohydrolase belonging to the serine peptidase family that catalyzes the hydrolysis of the terminal aminoacyl moiety of a polyketide intermediate during the biosynthesis of vicenistatin. Herein, we report the crystal structure of VinJ. VinJ possesses a unique hydrophobic tunnel for the recognition of the ...

journal_title：FEBS letters

authors： Shinohara Y,Miyanaga A,Kudo F,Eguchi T

更新日期：2014-03-18 00:00:00

abstract：:A central step in xanthone biosynthesis is the formation of the C13 skeleton, i.e. an intermediate benzophenone. Biosynthesis of 2,3',4,6-tetrahydroxybenzophenone from m-hydroxybenzoyl-CoA and malonyl-CoA was shown in cell-free extracts from cultured cells of Centaurium erythraea. The enzyme catalyzing this reaction w...

journal_title：FEBS letters

authors： Beerhues L

更新日期：1996-04-01 00:00:00

abstract：:In the rat liver, transcription of the serine:pyruvate/alanine:glyoxylate aminotransferase (SPT/AGT) gene occurs from two sites, +1 and +66, in exon 1, resulting in the formation of two mRNAs, one for a precursor of mitochondrial SPT/AGT and the other for peroxisomal SPT/AGT, respectively. In this study, we attempted ...

journal_title：FEBS letters

authors： Sugiyama T,Uchida C,Oda T,Kitagawa M,Hayashi H,Ichiyama A

更新日期：2001-11-09 00:00:00

abstract：:Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin affinity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identified by mass spectrometry as adenosine kinase. The ligand interaction reported here can be dis...

journal_title：FEBS letters

authors： Laukens K,Lenobel R,Strnad M,Van Onckelen H,Witters E

更新日期：2003-01-02 00:00:00

abstract：:Ceramide-1-phosphate (CER-1-P) is emerging as a novel bioactive sphingolipid. It is formed by phosphorylation of ceramide catalyzed by ceramide kinase, and has been implicated in different cellular processes. Cer-1-P is mitogenic for fibroblasts, blocks apoptosis in macrophages, controls phagocytosis in neutrophils, a...

journal_title：FEBS letters

authors： Gómez-Muñoz A

更新日期：2004-03-26 00:00:00

abstract：:Posttranslational modifications of circadian oscillator components are crucial for the generation of circadian rhythms. Among those phosphorylation plays key roles ranging from regulating degradation, complex formation, subcellular localization and activity. Although most of the known clock proteins are phosphoprotein...

journal_title：FEBS letters

authors： Reischl S,Kramer A

更新日期：2011-05-20 00:00:00

abstract：:More than 40 years have passed since the original publication by Hayflick and Moorhead led to the concept of the 'Hayflick limit' of the maximum number of divisions which somatic cells undergo in vitro. This concept is still regarded as a fundamental characteristic of species longevity. Here we want to emphasize anoth...

journal_title：FEBS letters

authors： Yegorov YE,Zelenin AV

更新日期：2003-04-24 00:00:00

abstract：:Treatment of the chemically transformed fibroblasts BP-A31 and other cell lines with low concentrations of cycloheximide (CHM) for 72 h followed by the removal of the protein synthesis inhibitor leads to the proliferation of alpha-difluoromethylornithine (DFMO)-resistant phenotypes. These drug-resistant cells contain ...

journal_title：FEBS letters

authors： Medrano EE,Burrone OR,Ferrer MM,Cafferata EG,Algranati ID

更新日期：1986-09-29 00:00:00

abstract：:Transport of the precursor for carbamoyl-phosphate synthetase I into mitochondria in Reuber hepatoma H-35 cells was inhibited by adding monensin or nigericin to the culture medium at a concentration of 0.5 microM, and the enzyme precursor accumulated, mainly in the cytosolic fraction. Accumulated precursor was degrade...

journal_title：FEBS letters

authors： Kitagawa Y,Murakami A,Sugimoto E

更新日期：1984-01-02 00:00:00

abstract：:A human teratoma cell line (Tera 2) was grown in serum-free medium, and the population multiplication was stimulated by the addition of 1-10 ng basic fibroblast growth factor (bFGF)/ml. The bFGF-effect was abrogated by the addition of protamine sulphate. When high concentrations of bFGF were added, a preferential effe...

journal_title：FEBS letters

authors： Schofield PN,Granerus M,Lee A,Ektröm TJ,Engström W

更新日期：1992-02-24 00:00:00

abstract：:Casein kinase Ialpha (CKIalpha) belongs to a family of serine/threonine protein kinases involved in membrane trafficking, RNA processing, mitotic spindle formation and cell cycle progression. In this report, we identified several CKIalpha interacting proteins including RCC1, high mobility group proteins 1 and 2 (HMG1,...

journal_title：FEBS letters

authors： Dubois T,Howell S,Zemlickova E,Aitken A

更新日期：2002-04-24 00:00:00

abstract：:For more than three decades, RNA recombination remained a puzzle and has only begun to be solved in the last few years. The available data provide evidence for a variety of RNA recombination mechanisms. Non-homologous recombination seems to be the most common for RNA. Recent experiments in both the in vitro and the in...

journal_title：FEBS letters

authors： Chetverin AB

更新日期：1999-10-22 00:00:00

abstract：:Vav functions as a specific GDP/GTP nucleotide exchange factor which is regulated by tyrosine phosphorylation in the hematopoietic system. Loss of the amino-terminus sequences of Vav was sufficient to control its transforming potential and its function in T cells. We report here the identification of the hematopoietic...

journal_title：FEBS letters

authors： Groysman M,Russek CS,Katzav S

更新日期：2000-02-04 00:00:00

abstract：:Peptic digestion of bovine hemoglobin yields a fragment with antibacterial activity. This peptide was purified to homogeneity by a two-step procedure including anion exchange chromatography and preparative reversed-phase HPLC. Mass determination and fragmentation indicated that this peptide corresponded to the 1-23 fr...

journal_title：FEBS letters

authors： Froidevaux R,Krier F,Nedjar-Arroume N,Vercaigne-Marko D,Kosciarz E,Ruckebusch C,Dhulster P,Guillochon D

更新日期：2001-02-23 00:00:00

abstract：:The kinetic properties of dUTPase from equine infectious anemia virus (EIAV) were investigated. K(M) (1.1 +/- 0.1 microM) and k(cat) (25 s(-1)) were found to be independent of pH in the neutral pH range. Above pH 8.0, K(M) increases slightly. Below pH 6.0, the enzyme is rapidly deactivated. Detergent was found to enha...

journal_title：FEBS letters

authors： Nord J,Larsson G,Kvassman JO,Rosengren AM,Nyman PO

更新日期：1997-09-08 00:00:00

abstract：:The modifying effects of the products of the equimolar addition Of DL-alanine and DL-alanyl-DL-alanine to fullerene C60 on the structure and permeability of the lipid bilayer of phosphatidylcholine liposomes has been studied using the luminescence probe technique. It is shown that these water soluble amino acid and di...

journal_title：FEBS letters

authors： Kotelnikova RA,Kotelnikov AI,Bogdanov GN,Romanova VS,Kuleshova EF,Parnes ZN,Vol'pin ME

更新日期：1996-07-01 00:00:00

abstract：:Zinc metallopeptidases that contain the His-Glu-Xaa-Xaa-His (HEXXH) motif generally have a third ligand of the metal ion that may be either a Glu residue (in clan MA) or a His residue (in clan MB) (Rawlings and Barrett (1995) Methods Enzymol. 248, 183-228). Thimet oligopeptidase has not yet been assigned to either cla...

journal_title：FEBS letters

authors： Chen JM,Stevens RA,Wray PW,Rawlings ND,Barrett AJ

更新日期：1998-09-11 00:00:00

abstract：:Selenium-dependent glutathione peroxidase activity is documented for the first time in insects. Reduction in glutathione peroxidase activity in the cytosol of adult house flies by lowering selenium in the diet results in significant increases in peroxidative injury. Catalase activity, while higher in low-selenium flie...

journal_title：FEBS letters

authors： Simmons TW,Jamall IS,Lockshin RA

更新日期：1987-06-29 00:00:00

abstract：:The crystal structure of Arthromyces ramosus peroxidase (ARP) in complex with benzhydroxamic acid (BHA) as determined by X-ray analysis at 1.6 A shows unambiguously how BHA binds to ARP. BHA is located in the distal heme pocket. Its functional groups are held by three hydrogen bonds to His56N(epsilon), Arg52N(epsilon)...

journal_title：FEBS letters

authors： Itakura H,Oda Y,Fukuyama K

更新日期：1997-07-21 00:00:00

abstract：:DNA bending is due to two main factors: the inherent curvature of the sequence and its flexibility. Most methods of analysis do not allow a differentiation between these two factors. In this paper I show that the flexibility of DNA sequences can be estimated from the standard deviation of roll values determined by X-r...

journal_title：FEBS letters

authors： Subirana JA

更新日期：1997-11-17 00:00:00

abstract：:The Ala-30 and Asn-32 residues involved in the major antiparallel beta-sheet structure of human epidermal growth factor (hEGF) were substituted with various amino acid residues, and the receptor-binding affinities of the nine variant hEGFs were determined by the use of human KB cells. The Ala-30----Arg, Ala-30----His ...

journal_title：FEBS letters

authors： Koide H,Muto Y,Kasai H,Hoshi K,Takusari H,Kohri K,Takahashi S,Sasaki T,Tsukumo K,Miyake T

更新日期：1992-05-04 00:00:00

abstract：:Insulin causes a translocation of glucose transporters from intracellular microsomes to the plasma membrane in adipocytes. To determine whether insulin has a similar effect in rat hindlimb muscles, we used glucose-inhibitable cytochalasin B binding to estimate the number of glucose transporters in membrane fractions f...

journal_title：FEBS letters

authors： Klip A,Ramlal T,Young DA,Holloszy JO

更新日期：1987-11-16 00:00:00

abstract：:Proteasomes were purified from human hepatoma tissues, and their sensitivities to Na+ and K+ were examined. At concentrations of 10 mM or more, these cations were found to inhibit completely polylysine-activated casein degradation by the purified proteasomes. They also strongly inhibited the hydrolyses of peptides, al...

journal_title：FEBS letters

authors： Seol JH,Park SC,Ha DB,Chung CH,Tanaka K,Ichihara A

更新日期：1989-04-24 00:00:00

abstract：:Leaves and chloroplasts from Arabidopsis plants with increased amounts of PsbS protein showed the same percentage increase in nonphotochemical quenching in comparison to the wild type both in the presence and absence of zeaxanthin. The absorption change at 525-535 nm was also more pronounced in both cases. It is sugge...

journal_title：FEBS letters

authors： Crouchman S,Ruban A,Horton P

更新日期：2006-04-03 00:00:00