Abstract:
:Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin affinity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identified by mass spectrometry as adenosine kinase. The ligand interaction reported here can be disrupted by several other adenine- but not guanine-based purine derivatives. The observed interaction with cytokinins is discussed in view of a putative role for adenosine kinase in TBY-2 cytokinin metabolism. The presented results show for the first time a plant adenosine kinase affinity-purified to homogeneity that was identified by primary structure analysis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Laukens K,Lenobel R,Strnad M,Van Onckelen H,Witters Edoi
10.1016/s0014-5793(02)03750-xkeywords:
subject
Has Abstractpub_date
2003-01-02 00:00:00pages
63-6issue
1-3eissn
0014-5793issn
1873-3468pii
S001457930203750Xjournal_volume
533pub_type
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