Abstract:
:Peptic digestion of bovine hemoglobin yields a fragment with antibacterial activity. This peptide was purified to homogeneity by a two-step procedure including anion exchange chromatography and preparative reversed-phase HPLC. Mass determination and fragmentation indicated that this peptide corresponded to the 1-23 fragment of the alpha chain of hemoglobin. The minimum inhibitory concentration and mode of action of this peptide towards Micrococcus luteus strain A270 were determined. Hemolytic assay, interaction with liposomes, and study of its structure in solution were also performed.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Froidevaux R,Krier F,Nedjar-Arroume N,Vercaigne-Marko D,Kosciarz E,Ruckebusch C,Dhulster P,Guillochon Ddoi
10.1016/s0014-5793(01)02171-8keywords:
subject
Has Abstractpub_date
2001-02-23 00:00:00pages
159-63issue
1-2eissn
0014-5793issn
1873-3468pii
S0014-5793(01)02171-8journal_volume
491pub_type
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