Abstract:
:A complete cDNA encoding rabbit uteroglobin was constructed and expressed in MDCK and Caco-2 cells. The MDCK cells secrete uteroglobin in approximately equal amounts to the apical and the basolateral side, whereas the Caco-2 cells secrete uteroglobin mainly to the basolateral side. Both MDCK and Caco-2 cells thus secrete uteroglobin in a non-sorted manner. It has, however, previously been shown that uteroglobin is secreted exclusively at the apical membrane in primary cell culture of endometrial epithelial cells [S.K. Mani et al. (1991) Endocrinology 128, 1563-1573]. This suggests that either the endometrial epithelium has an apical default pathway or recognises a sorting signal not recognised by MDCK cells and Caco-2 cells. Our data thus show that a soluble molecule can be secreted at the apical, the basolateral or both membranes depending on the cell type.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Vogel LK,Suske G,Beato M,Norén O,Sjöström Hdoi
10.1016/0014-5793(93)80891-wsubject
Has Abstractpub_date
1993-09-20 00:00:00pages
293-6issue
3eissn
0014-5793issn
1873-3468pii
001457939380891Wjournal_volume
330pub_type
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