Abstract:
:Strigolactones are a new class of phytohormones synthesized from carotenoids via carlactone. The complex structure of carlactone is not easily deducible from its precursor, a cis-configured β-carotene cleavage product, and is thus formed via a poorly understood series of reactions and molecular rearrangements, all catalyzed by only one enzyme, the carotenoid cleavage dioxygenase 8 (CCD8). Moreover, the reactions leading to carlactone are expected to form a second, yet unidentified product. In this study, we used 13 C and 18 O-labeling to shed light on the reactions catalyzed by CCD8. The characterization of the resulting carlactone by LC-MS and NMR, and the identification of the assumed, less accessible second product allowed us to formulate a minimal reaction mechanism for carlactone generation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Bruno M,Vermathen M,Alder A,Wüst F,Schaub P,van der Steen R,Beyer P,Ghisla S,Al-Babili Sdoi
10.1002/1873-3468.12593subject
Has Abstractpub_date
2017-03-01 00:00:00pages
792-800issue
5eissn
0014-5793issn
1873-3468journal_volume
591pub_type
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