Abstract:
:The biosynthesis of human tumor necrosis factor (hTNF) was studied. The amino-terminal extension of the hTNF precursor (26 kDa polypeptide) was not cleaved off in a cell-free system supplemented with dog pancreas microsomes. Correct maturation of pre-hTNF was nevertheless not restricted to the macrophage system: in the medium of a TNF-producing, transformed CHO cell line, a (weak) approximately 20 kDa, an approximately 18.5 kDa (doublet) and a 17 kDa TNF polypeptide, the latter corresponding to mature hTNF, were revealed by specific immunoprecipitation. Similar results were obtained with Xenopus laevis oocytes, injected with hTNF mRNA, except that the 20 kDa band was lacking. The results are discussed in relation to the secretion mechanism of hTNF.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Müller R,Marmenout A,Fiers Wdoi
10.1016/0014-5793(86)80306-4subject
Has Abstractpub_date
1986-03-03 00:00:00pages
99-104issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(86)80306-4journal_volume
197pub_type
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