Abstract:
:In order to study the transactivational property of the X gene in the methylotropic yeast Pichia pastoris, a Rous sarcoma virus-chloramphenicol acetyltransferase (RSV-CAT) cassette was co-transformed and integrated into the host yeast strain as a reporter which showed an overwhelming CAT activity. Immunoprecipitation of the yeast cell extracts with an X-specific monoclonal antibody, however, showed a low level expression of the X gene. Therefore besides a trans-effect of the X protein, the enhanced reporter activity could be a manifestation of a cis-effect of the X gene sequences also. Therefore, unlike the transactivation studies with X gene in animal cells where limited functional activity is observed, P. pastoris appears to be an excellent system to study cis- and trans-aspects of gene regulation by the X gene.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Lal SK,Kumar Vdoi
10.1016/s0014-5793(99)00930-8keywords:
subject
Has Abstractpub_date
1999-07-30 00:00:00pages
108-12issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00930-8journal_volume
456pub_type
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