Abstract:
:The intracellular expression of recombinant antibodies in mammalian cells is an experimental strategy to inhibit in vivo the function of selected molecules. However, one limitation of this technology is represented by the unpredictable behaviour of antibodies, under conditions of intracellular expression. For this reason, it would be desirable to exploit intracellular expression of antibodies to select or rescue more efficient ones from a polyclonal mixture. In this work we have successfully explored this possibility by rescuing a neutralizing anti-viral antibody fragment from an intracellularly expressed anti-reverse transcriptase polyclonal repertoire.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Gargano N,Cattaneo Adoi
10.1016/s0014-5793(97)01065-xsubject
Has Abstractpub_date
1997-09-15 00:00:00pages
537-40issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(97)01065-Xjournal_volume
414pub_type
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