Time-resolved Fourier-transform infrared studies of the cytochrome P-450cam carbonmonoxide complex bound with (1R)-camphor and (1S)-camphor substrate.


:The CO-binding reaction of cytochrome P-450cam bound with (1R)-camphor and (1S)-camphor are compared in the temperature region of 210-260 K using time-resolved Fourier-transform infrared spectroscopy with the CO stretch vibration as spectroscopic probe. For (1S)-camphor as substrate the association of CO is slowed down by a factor of 2, while the dissociation is accelerated by a factor of 3. The CO complex for the (1S)-camphor-bound P-450 is less stabilized (deltaG=-22 kJ/mol) compared to the natural substrate (1R)-camphor (deltaG=-30 kJ/mol). The data are interpreted by a smaller change of the mobility of the (1S)-camphor due to CO binding as compared to (1R)-camphor, which would indicate a higher mobility of (1S)-camphor already in the CO free reduced form of P-450cam. The higher mobility of (1S)-camphor in the heme pocket might explain the increased uncoupling rate (hydrogen peroxide formation) of 11% [Maryniak et al. (1993) Tetrahedron 49, 9373-9384] during the P-450cam catalyzed hydroxylation compared to 3% for the conversion of (1R)-camphor.




FEBS letters


Contzen J,Ristau O,Jung C




Has Abstract


1996-03-25 00:00:00














  • Tyrosine phosphorylation and translocation of LAT in platelets.

    abstract::Platelet aggregation is accompanied by the tyrosine phosphorylation of several proteins including syk. However, some of these proteins are not identified. Recent studies showed that LAT is a syk substrate and is tyrosine phosphorylated during T cell stimulation. In this study, we demonstrated that LAT is present in pl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sarkar S

    更新日期:1998-12-28 00:00:00

  • UCN-01, an anti-tumor drug, is a selective inhibitor of the conventional PKC subfamily.

    abstract::A selective PKC inhibitor, UCN-01, was shown to exhibit anti-tumor activity in vitro and in vivo. We investigated UCN-01 with respect to isozyme-specific PKC inhibition using purified recombinant or rabbit brain PKC isozymes, cPKC alpha, beta and gamma, nPKC delta, epsilon and eta, and a PKC zeta. Of the PKC isozymes ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mizuno K,Noda K,Ueda Y,Hanaki H,Saido TC,Ikuta T,Kuroki T,Tamaoki T,Hirai S,Osada S

    更新日期:1995-02-13 00:00:00

  • Molecular cloning of a novel C or gamma type chemokine, SCM-1.

    abstract::From human PBMC stimulated with PHA, we have isolated cDNA clones encoding a novel cytokine named SCM-1, which is significantly related to the CC and the CXC chemokines but has only the 2nd and the 4th of the four cysteines conserved in these proteins. Its gene is also distinctly mapped to human chromosome 1. SCM-1 is...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yoshida T,Imai T,Kakizaki M,Nishimura M,Yoshie O

    更新日期:1995-02-27 00:00:00

  • Capsaicin, a component of red peppers, induces expression of androgen receptor via PI3K and MAPK pathways in prostate LNCaP cells.

    abstract::In this study, capsaicin (trans-8-methyl-N-vanillyl-6-nonenamide) induced an increase in the cell viability of the androgen-responsive prostate cancer LNCaP cells, which was reversed by the use of the TRPV1 antagonists capsazepine, I-RTX and SB 366791. In further studies we observed that capsaicin induced a decrease i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Malagarie-Cazenave S,Olea-Herrero N,Vara D,Díaz-Laviada I

    更新日期:2009-01-05 00:00:00

  • The role of apoE in inhibitory effects of apoE-rich HDL on platelet function.

    abstract::Apolipoprotein E- (ApoE-) rich high-density lipoprotein (HDL), which was prepared from the bound fraction of normolipemic volunteers on heparin-Sepharose and from a hyperalphalipoproteinemic patient, potently inhibited aggregation of human platelets in a dose-dependent fashion. Dimyristoyl phosphatidylcholine liposome...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Higashihara M,Kinoshita M,Teramoto T,Kume S,Kurokawa K

    更新日期:1991-04-22 00:00:00

  • Differential role of PPAR gamma in the regulation of UCP-1 and adipogenesis by TNF-alpha in brown adipocytes.

    abstract::Extracellular regulated kinases (ERKs) mediate the inhibitory effect of tumor necrosis factor alpha (TNF-alpha) on uncoupling protein-1 (UCP-1), but not on lipid accumulation. TNF-alpha-induced ERK-dependent peroxisome proliferator activator receptor gamma (PPAR gamma) phosphorylation could be responsible for UCP-1 do...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Porras A,Valladares A,Alvarez AM,Roncero C,Benito M

    更新日期:2002-06-05 00:00:00

  • RanBPM is expressed in synaptic layers of the mammalian retina and binds to metabotropic glutamate receptors.

    abstract::In the central nervous system, synaptic signal transduction depends on the regulation of neurotransmitter receptors by interacting proteins. Here, we searched for proteins interacting with two metabotropic glutamate receptor type 8 isoforms (mGlu8a and mGlu8b) and identified RanBPM. RanBPM is expressed in several brai...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Seebahn A,Rose M,Enz R

    更新日期:2008-07-09 00:00:00

  • Changes in L-ornithine decarboxylase activity in regenerating lung lobes.

    abstract::Growth of regenerating lung lobes is preceded by an increase of the activity of L-ornithine decarboxylase (ODC). Mechanical factors play a role in determining both the growth rate and enhancement of activity of ODC in regenerating lung lobes. Hormonal regulation also appears of importance as shown by the increased ODC...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tatár-Kiss S,Bardócz S,Kertai P

    更新日期:1984-09-17 00:00:00

  • Single electron reduction of 'slow' and 'fast' cytochrome-c oxidase.

    abstract::Evidence is presented that single electron reduction is sufficient for rapid electron transfer (k greater than 20 s-1 at pH 8.0 in 0.43 M potassium EDTA) between haem a/CuA and the binuclear centre in 'fast' oxidase, whereas in 'slow' oxidase intramolecular electron transfer is slow even when both CuA and haem a are r...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Moody AJ,Brandt U,Rich PR

    更新日期:1991-11-18 00:00:00

  • IL-1 beta, a strong mediator for glucose uptake by rheumatoid and non-rheumatoid cultured human synoviocytes.

    abstract::Higher basal 2-deoxy-D-glucose uptake in rheumatoid synovial cells than in non-rheumatoid synovial cells, was found to be associated with an increased interleukin-1 beta (IL-1 beta) secretion (respectively 850 +/- 238 vs. 8.3 +/- 2.4 pg/24 h/10(5) cells, mean +/- S.E.M.). When exogenous human recombinant IL-1 beta was...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hernvann A,Aussel C,Cynober L,Moatti N,Ekindjian OG

    更新日期:1992-05-25 00:00:00

  • Identification of creatine kinase isoenzymes in the guinea-pig. Presence of mitochondrial creatine kinase in smooth muscle.

    abstract::Isoenzymes of creatine kinase (CK, EC in guinea-pig smooth, cardiac and skeletal muscles as well as in brain were analyzed by cellulose acetate electrophoresis and FPLC gel permeation chromatography. In crude tissue extracts of smooth muscles brain type BB-CK and the hybrid form MB-CK were detected, but in en...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ishida Y,Wyss M,Hemmer W,Wallimann T

    更新日期:1991-05-20 00:00:00

  • Interaction between human amphipathic apolipoproteins and amyloid beta-peptide: surface plasmon resonance studies.

    abstract::Several apolipoproteins including apoE and apoA-I are known to be associated with amyloid beta-peptide, a major component of senile plaques in Alzheimer's disease. In the present study the interaction between three human amphipathic apolipoproteins apoE3, apoA-I and apoA-II and immobilized amyloid beta-peptide (1-40) ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Shuvaev VV,Siest G

    更新日期:1996-03-25 00:00:00

  • Psoralen photofootprinting of protein-binding sites on DNA.

    abstract::Using a BAL31 exonuclease assay to determine the sites of 4,5',8-trimethylpsoralen photocrosslinking in DNA we have shown that 5'-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, lambda-repressor E. coli or RNA polymerase are bound at t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zhen WP,Jeppesen C,Nielsen PE

    更新日期:1988-02-29 00:00:00

  • Substituting selenocysteine for active site cysteine 149 of phosphorylating glyceraldehyde 3-phosphate dehydrogenase reveals a peroxidase activity.

    abstract::Replacing the essential Cys-149 by a selenocysteine into the active site of phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPDH) from Bacillus stearothermophilus leads to a selenoGAPDH that mimics a selenoperoxidase activity. Saturation kinetics were observed with cumenyl and tert-butyl hydroperoxides, wit...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Boschi-Muller S,Muller S,Van Dorsselaer A,Böck A,Branlant G

    更新日期:1998-11-20 00:00:00

  • Specific inhibition of human beta-D-glucuronidase and alpha-L-iduronidase by a trihydroxy pipecolic acid of plant origin.

    abstract::The glucuronic acid analogue of 1-deoxynojirimycin, 2(S)-carboxy-3(R), 4(R), 5(S)-trihydroxypiperidine, recently isolated from seeds of Baphia racemosa, is a novel specific inhibitor of human liver beta-D-glucuronidase and alpha-L-iduronidase. No other glycosidases are inhibited. The inhibition of beta-D-glucuronidase...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cenci di Bello I,Dorling P,Fellows L,Winchester B

    更新日期:1984-10-15 00:00:00

  • Identification of region-specific transcription factor genes in the adult mouse brain by medium-scale real-time RT-PCR.

    abstract::We established a medium-scale real-time RT-PCR system focusing on transcription factors and applied it to their expression profiles in the adult mouse 11 brain regions (http://genome.gsc.riken.jp/qRT-PCR/). Almost 90% of the examined genes showed significant expression in at least one region. We successfully extracted...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Suzuki H,Okunishi R,Hashizume W,Katayama S,Ninomiya N,Osato N,Sato K,Nakamura M,Iida J,Kanamori M,Hayashizaki Y

    更新日期:2004-08-27 00:00:00

  • Investigation of H2AX methylation by the SUV39H2 protein lysine methyltransferase.

    abstract::The H3K9 protein lysine methyltransferase SUV39H2 was reported to methylate K134 of H2AX and stimulate H2AX phosphorylation during DNA damage response [Sone K et al. (2014) Nat Commun 5, 5691]. However, the sequence context of H2AX-K134 differs from the specificity of SUV39H2. We performed in vitro methylation reactio...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schuhmacher MK,Kudithipudi S,Jeltsch A

    更新日期:2016-06-01 00:00:00

  • Activation of oxidative stress response by hydroxyl substituted chalcones and cyclic chalcone analogues in mitochondria.

    abstract::We investigated the effect of hydroxyl substituted chalcone (1a) and some chalcone analogues (1b-d) on isolated rat liver mitochondria to gain new insights into the cytotoxic mechanism of these compounds. We observed an inhibitory effect on phosphorylation and the partial uncoupling of compounds 1a and 1d. Increased r...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Guzy J,Vasková-Kubálková J,Rozmer Z,Fodor K,Mareková M,Poskrobová M,Perjési P

    更新日期:2010-02-05 00:00:00

  • Amylin injection causes elevated plasma lactate and glucose in the rat.

    abstract::Intravenous injections of 25.5 nmol rat amylin into fasted anesthetized rats caused a rapid increase in plasma lactate followed by an increase in plasma glucose; there was a transient fall in blood pressure. Subcutaneous injection of 25.5 nmol amylin also caused increases in lactate and glucose but did not change bloo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Young AA,Wang MW,Cooper GJ

    更新日期:1991-10-07 00:00:00

  • Plasma 8-epi-PGF2 alpha levels are elevated in individuals with non-insulin dependent diabetes mellitus.

    abstract::This study reports plasma levels of a specific nonenzymatic peroxidation product of arachidonic acid, esterified 8-epi-PGF2 alpha, from healthy- and NIDDM individuals as an index of oxidative stress in vivo. Plasma 8-epi-PGF2 alpha was isolated by solid-phase extraction on a C18 followed by an NH2 cartridge and analyz...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gopaul NK,Anggård EE,Mallet AI,Betteridge DJ,Wolff SP,Nourooz-Zadeh J

    更新日期:1995-07-17 00:00:00

  • Requirements for a conservative protein translocation pathway in chloroplasts.

    abstract::The chloroplast inner envelope translocon subunit Tic110 is imported via a soluble stromal translocation intermediate. In this study an in-organellar import system is established which allows for an accumulation of this intermediate in order to analyze its requirements for reexport. All results demonstrate that the re...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vojta L,Soll J,Bölter B

    更新日期:2007-06-12 00:00:00

  • Site-directed mutagenesis of alanine-382 of human antithrombin III.

    abstract::Antithrombin III Hamilton is a structural variant of antithrombin III (AT-III) with normal heparin affinity but impaired serine protease inhibitory activity. The molecular defect of AT-III-Hamilton is a substitution of threonine for alanine at amino acid residue 382. Recently it has been shown that both plasma-derived...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Austin RC,Rachubinski RA,Blajchman MA

    更新日期:1991-03-25 00:00:00

  • Robustness, evolvability, and neutrality.

    abstract::Biological systems, from macromolecules to whole organisms, are robust if they continue to function, survive, or reproduce when faced with mutations, environmental change, and internal noise. I focus here on biological systems that are robust to mutations and ask whether such systems are more or less evolvable, in the...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wagner A

    更新日期:2005-03-21 00:00:00

  • Rapid purification of bovine kidney branched-chain 2-oxoacid dehydrogenase complex containing endogenous kinase activity.

    abstract::Branched-chain 2-oxoacid dehydrogenase complex has been purified to near homogeneity by a simple, rapid procedure. The final product contains endogenous kinase activity capable of phosphorylating and inactivating the complex. Phosphorylation continues after complete inactivation, indicating the possibility of several ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lawson R,Cook KG,Yeaman SJ

    更新日期:1983-06-27 00:00:00

  • The Drosophila cation channel trpl expressed in insect Sf9 cells is stimulated by agonists of G-protein-coupled receptors.

    abstract::Structures and regulations of vertebrate channels responsible for sustained calcium elevations after hormone stimulation are largely unknown. Therefore, the Drosophila photoreceptor channels, trp and trpl, which are assumed to be involved in calcium influx, serve as model system, trpl expressed in Sf9 cells showed spo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Harteneck C,Obukhov AG,Zobel A,Kalkbrenner F,Schultz G

    更新日期:1995-01-30 00:00:00

  • Agarose gel electrophoretic evidence for domains of nuclear DNA linked with bonds cleavable with sulfhydryl molecules.

    abstract::Complexes of intact nuclear DNA with proteins undissociable by 2.0 M NaCl and nonionic detergents were analyzed by agarose gel electrophoresis following physical or enzymatic fragmentation. Sulfhydryl molecules converted these DNAs (but not the bacteriophage lambda DNA) into smaller-Mr forms. Following limited restric...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Taş S,De Larco J,Altiok E

    更新日期:1985-10-21 00:00:00

  • Haemozoin (beta-haematin) biomineralization occurs by self-assembly near the lipid/water interface.

    abstract::Several blood-feeding organisms, including the malaria parasite detoxify haem released from host haemoglobin by conversion to the insoluble crystalline ferriprotoporphyrin IX dimer known as haemozoin. To date the mechanism of haemozoin formation has remained unknown, although lipids or proteins have been suggested to ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Egan TJ,Chen JY,de Villiers KA,Mabotha TE,Naidoo KJ,Ncokazi KK,Langford SJ,McNaughton D,Pandiancherri S,Wood BR

    更新日期:2006-09-18 00:00:00

  • Demonstration of cell-specific phosphorylation of LTC4 synthase.

    abstract::PMA-induced leukotriene C4 synthase (LTC4S) phosphorylation was investigated over a period of 8 h in a monocytic cell line (THP-1). The level of LTC4S phosphorylation was increased 3-5 fold over a 4 h period decreasing to basal levels after 8 h. This phosphorylation event was found to be specific to THP-1 cells as the...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gupta N,Nicholson DW,Ford-Hutchinson AW

    更新日期:1999-04-16 00:00:00

  • Heterologous coexpression of the blue light receptor psRII and its transducer pHtrII from Natronobacterium pharaonis in the Halobacterium salinarium strain Pho81/w restores negative phototaxis.

    abstract::The photophobic receptor (psRII) and its transducer pHtrII from Natronobacterium pharaonis were heterologously coexpressed in the phototaxis-deficient Halobacterium salinarium strain Pho81/w which lacks all four bacterial rhodopsins, i.e. the two ion pumps bacteriorhodopsin and halorhodopsin as well as the two sensory...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lüttenberg B,Wolff EK,Engelhard M

    更新日期:1998-04-10 00:00:00

  • Phosphatidic acid metabolism in rat liver cell nuclei.

    abstract::The aim of the present research was to analyze the pathways for phosphatidic acid metabolism in purified nuclei from liver. Lipid phosphate phosphatase, diacylglycerol lipase, monoacylglycerol lipase and PA-phospholipase type A activities were detected. The presence of lysophosphatidic acid significantly reduced DAG p...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gaveglio VL,Pasquaré SJ,Giusto NM

    更新日期:2013-04-02 00:00:00