Abstract:
:The CO-binding reaction of cytochrome P-450cam bound with (1R)-camphor and (1S)-camphor are compared in the temperature region of 210-260 K using time-resolved Fourier-transform infrared spectroscopy with the CO stretch vibration as spectroscopic probe. For (1S)-camphor as substrate the association of CO is slowed down by a factor of 2, while the dissociation is accelerated by a factor of 3. The CO complex for the (1S)-camphor-bound P-450 is less stabilized (deltaG=-22 kJ/mol) compared to the natural substrate (1R)-camphor (deltaG=-30 kJ/mol). The data are interpreted by a smaller change of the mobility of the (1S)-camphor due to CO binding as compared to (1R)-camphor, which would indicate a higher mobility of (1S)-camphor already in the CO free reduced form of P-450cam. The higher mobility of (1S)-camphor in the heme pocket might explain the increased uncoupling rate (hydrogen peroxide formation) of 11% [Maryniak et al. (1993) Tetrahedron 49, 9373-9384] during the P-450cam catalyzed hydroxylation compared to 3% for the conversion of (1R)-camphor.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Contzen J,Ristau O,Jung Cdoi
10.1016/0014-5793(96)00103-2subject
Has Abstractpub_date
1996-03-25 00:00:00pages
13-7issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(96)00103-2journal_volume
383pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The trichothecene 3-O-acetyltransferase gene, Tri101, plays a pivotal role for the well-being of the type B trichothecene producer Fusarium graminearum. We have analyzed the cosmids containing Tri101 and found that this resistance gene is not in the biosynthetic gene cluster reported so far. It was located between the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01061-8
更新日期:1998-09-18 00:00:00
abstract::The temperature dependence of the rate constants of the univalent redox steps YzoxSi----YzSi + 1 (i = 0,1,2) and YzoxS3----(YzS4)----YzSo + O2 in the water oxidase was investigated by measuring time resolved absorption changes at 355 nm induced by a laser flash train in dark adapted PS II membrane fragments from spina...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80092-u
更新日期:1992-03-24 00:00:00
abstract::EcL-DER, the aspartate/glutamate racemase from the pathogen Escherichia coli O157, exhibits racemase activity for l-aspartate and l-glutamate. This study reports the crystal structures of apo-EcL-DER, the EcL-DER-l-aspartate and the EcL-DER-d-aspartate complexes. The EcL-DER structure contains two domains, forming pse...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12148
更新日期:2016-04-01 00:00:00
abstract::Connivance of cellular factors during virus-host cell membrane fusion is poorly understood. We have recently shown that cellular villin plays an important role during membrane fusion of reconstituted Sendai virosomes with hepatocytes. Here, we employed villin-null Chinese Hamster Ovary (CHO) cells, where villin expres...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13477
更新日期:2019-08-01 00:00:00
abstract::The effect of estramustine and estramustine phosphate (EP) on the interaction of microtubule associated protein 2 (MAP2) with actin has been examined. We show that (a) neither estramustine nor EP influences actin polymerisation (b) EP, but not estramustine, reduces the amount of MAP2 which co-sediments with F-actin in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01524-4
更新日期:1997-02-17 00:00:00
abstract::Tau exon 10 (E10) splicing is a crucial event in its developmental change of tau isoform and tauopathy. To investigate the splicing mechanism, we isolated and compared mouse tau genomic sequence with human sequence. We identified a new element in mouse intron 10 (I10) to suppress E10 splicing, which was located just a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.11.090
更新日期:2005-01-03 00:00:00
abstract::The influence of islet-activating protein (IAP), a Bordetella pertussis toxin, was studied on adenylate cyclase and GTPase activities in rat adipocyte membranes. Pretreatment of rats or intact rat adipocytes with IAP did not affect adenylate cyclase inhibition by the stable GTP analog, GTP gamma S, whereas inhibition ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80254-3
更新日期:1983-05-30 00:00:00
abstract::Protein gene product 9.5 (PGP 9.5), which in the normal nervous system is restricted to certain neurons, has been detected in two glioma cell lines, rat C6 and human GL15, by immunoblotting and immunocytochemistry. Its expression in these cells depends on the cellular growth state, being maximal between the first and ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81242-z
更新日期:1991-09-23 00:00:00
abstract::A novel potassium channel beta-subunit (Kv beta 3) was cloned from rat brain being the third member of a Kv beta subunit gene family. It is a protein of 403 amino acid residues with a 68% amino acid sequence homology to Kv beta 1.1. Kv beta 3 is primarily expressed in rat brain having a distribution distinct to those ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01377-6
更新日期:1995-12-27 00:00:00
abstract::Human class Alpha glutathione transferase (GST) A1-1 has been subjected to site-directed mutagenesis of a Tyr residue conserved in all classes of cytosolic GSTs. The change of Tyr8----Phe lowers the specific activities with three substrates to 2-8% of the values for the wild-type enzyme. The changes in the kinetic par...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81174-7
更新日期:1991-11-18 00:00:00
abstract::Reliably pinpointing which specific amino acid residues form the interface(s) between a protein and its binding partner(s) is critical for understanding the structural and physicochemical determinants of protein recognition and binding affinity, and has wide applications in modeling and validating protein interactions...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2015.10.003
更新日期:2015-11-30 00:00:00
abstract::A 32 kDa phospholipase A2 inhibitory protein was isolated from pig thyroid gland after calcium precipitation and fast protein liquid anion-exchange chromatography. SDS-polyacrylamide gel electrophoresis revealed the purity of the protein. The protein activity was assessed by the inhibition of pancreatic phospholipase ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81273-0
更新日期:1988-08-01 00:00:00
abstract::Various properties have been evaluated for the binding to tissue culture substrata of proteolytic fragments of human plasma or cellular fibronectins containing complementary sequences from the individual and alternatively spliced chains, since related fragments are known to yield differing adhesive responses from cell...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80166-2
更新日期:1988-09-12 00:00:00
abstract::A pulsed field gradient three-dimensional isotope-filtered 13C HMQC-NOESY experiment has been developed to characterize intermolecular contacts in a 37 kDa macromolecular ternary complex consisting of uniformly 13C labeled trp-repressor, its natural abundance co-repressor, L-tryptophan, and natural abundance operator ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00740-3
更新日期:1994-08-15 00:00:00
abstract::The alpha-subunit of the TRAP complex (TRAP alpha) is a single-spanning membrane protein of the endoplasmic reticulum (ER) which is found in proximity of nascent polypeptide chains translocating across the membrane. Here, we demonstrate the widespread occurrence of TRAP alpha in eukaryotes as indicated by its existenc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00693-8
更新日期:1994-08-08 00:00:00
abstract::Phospholipase C(gamma)2 (PLCgamma2) is a phospholipid-converting enzyme which, upon receptor stimulation, is activated within membrane-bound signalling complexes. In contrast to the highly ubiquitous PLCgamma1, PLCgamma2 is expressed predominantly in B-lymphocytes. Associated with antigen-coupling receptors it is acti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01276-8
更新日期:1996-12-09 00:00:00
abstract::The accumulation of protoporphyrin IX (Proto IX) in light-sensitive mutants of Escherichia coli was detected by spectrofluorimetry. Fluorescence emission and excitation spectra were recorded from extracts of bacterial cells. Proto IX clearly accumulated in cells with mutations in the visA (hemH) gene but not in the wi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81341-i
更新日期:1992-10-05 00:00:00
abstract::African horse sickness virus (AHSV), a member of the orbivirus genus of the family Reoviridae, is an insect-vectored pathogen of horses of concern to the equine industry. Studies on AHSV replication and pathogenesis have been hampered by the lack of reverse genetics allowing targeted mutation of viral genomes. We demo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.06.030
更新日期:2010-08-04 00:00:00
abstract::Recently, the existence and extended diversity of antimicrobial peptides has been revealed in two mussel species. These molecules are classified into four groups according to common features of their primary structure: defensins, mytilins, myticins and mytimycin. In Mytilus galloprovincialis, gene structure reveals sy...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(00)02192-x
更新日期:2000-12-15 00:00:00
abstract::The requirement for a proline residue at the C-terminus of the Ca(2+)-binding photoprotein, aequorin, was investigated by measuring luminescence activities of a series of C-terminal deletion mutants, substitution mutants and an addition mutant. CD spectral measurements of apoaequorin with the C-terminal proline delete...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81385-l
更新日期:1991-12-16 00:00:00
abstract::The biological activity and the fate of SV40 DNA (minichromosomes, DNA I, DNA II, DNA III) were tested in culture cells by immunofluorescence staining and blot analysis. Following microinjection of 2-4 circular SV40 molecules (minichromosomes, DNA I, DNA II) into the cytoplasm or the nuclei of monkey and rat cells, T-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80187-3
更新日期:1985-01-01 00:00:00
abstract::The action of substrate analogs containing the tetrazolyl group instead of the C-terminal carboxy group on the peptidase activity of carboxypeptidase A is studied. The analogs compete with the substrate for the secondary binding site thus showing activation phenomena. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81561-2
更新日期:1987-02-09 00:00:00
abstract::Iron regulatory protein 2 (IRP2) is a critical switch for cellular and systemic iron homeostasis. In iron-deficient or hypoxic cells, IRP2 binds to mRNAs containing iron responsive elements (IREs) and regulates their expression. Iron promotes proteasomal degradation of IRP2 via the F-box protein FBXL5. Here, we explor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.01.036
更新日期:2011-02-18 00:00:00
abstract::Homeodomain-interacting protein kinase 2 (HIPK2) interacts with and phosphorylates various transcription factors that are critical regulators of cell fate decisions and apoptosis during development. Here we show that lysine 25 of HIPK2 is the major sumoylation site, both in vitro and in vivo, and that the sumoylation ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.04.053
更新日期:2005-06-06 00:00:00
abstract::In exocrine acinar cells a variety of neurotransmitters (e.g. acetylcholine) stimulate phosphatidylinositol 4,5-bisphosphate hydrolysis elevating intracellular calcium to activate calcium-dependent membrane currents (outward K+ and inward Cl-). This study shows that in lacrimal acinar cells extracellular application o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80782-e
更新日期:1990-05-07 00:00:00
abstract::alpha-1-Antitrypsin is found in hepatocytes as a high-mannose glycoprotein (Mr 49 000), extracellularly as a complex-type glycoprotein (Mr 54 000). Deglycosylation of both forms with peptide: N-glycosidase led to proteins of identical app. Mr (41 000). The sequence of 26 N-terminal amino acids of rat alpha 1-antitryps...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80069-6
更新日期:1983-01-24 00:00:00
abstract::Data obtained from peptide mapping of the active and inactive forms of human renin show that there are extensive regions of common sequence in the two forms of the enzyme, and are consistent with the hypothesis that inactive renin is a renin zymogen. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80784-x
更新日期:1984-10-01 00:00:00
abstract::Sperm beta-acrosin activity is inhibited by suramin, a polysulfonated naphthylurea compound with therapeutic potential as a combined antifertility agent and microbicide. A kinetic analysis of enzyme inhibition suggests that three and four molecules of suramin bind to one molecule of ram and boar beta-acrosins respecti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00488-5
更新日期:2003-06-05 00:00:00
abstract::The amino acid sequence of the alpha-subunit of Gi, the human adenylate cyclase inhibiting GTP-binding protein, has been deduced from the nucleotide sequence of a DNA clone complementary to Gi alpha mRNA from differentiated U937 cells. The cDNA encodes a polypeptide of 355 amino acids (Mr 40456). The amino acid sequen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81428-x
更新日期:1987-01-26 00:00:00
abstract::The rise of antibiotic resistance combined with the lack of new products entering the market has led to bacterial infections becoming one of the biggest threats to global health. Therefore, there is an urgent need to identify novel antibiotic targets, such as dihydrodipicolinate synthase (DHDPS), an enzyme involved in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13733
更新日期:2020-05-01 00:00:00