Time-resolved Fourier-transform infrared studies of the cytochrome P-450cam carbonmonoxide complex bound with (1R)-camphor and (1S)-camphor substrate.


:The CO-binding reaction of cytochrome P-450cam bound with (1R)-camphor and (1S)-camphor are compared in the temperature region of 210-260 K using time-resolved Fourier-transform infrared spectroscopy with the CO stretch vibration as spectroscopic probe. For (1S)-camphor as substrate the association of CO is slowed down by a factor of 2, while the dissociation is accelerated by a factor of 3. The CO complex for the (1S)-camphor-bound P-450 is less stabilized (deltaG=-22 kJ/mol) compared to the natural substrate (1R)-camphor (deltaG=-30 kJ/mol). The data are interpreted by a smaller change of the mobility of the (1S)-camphor due to CO binding as compared to (1R)-camphor, which would indicate a higher mobility of (1S)-camphor already in the CO free reduced form of P-450cam. The higher mobility of (1S)-camphor in the heme pocket might explain the increased uncoupling rate (hydrogen peroxide formation) of 11% [Maryniak et al. (1993) Tetrahedron 49, 9373-9384] during the P-450cam catalyzed hydroxylation compared to 3% for the conversion of (1R)-camphor.




FEBS letters


Contzen J,Ristau O,Jung C




Has Abstract


1996-03-25 00:00:00














  • Vp165 and GLUT4 share similar vesicle pools along their trafficking pathways in rat adipose cells.

    abstract::vp165 (or gp160) is an aminopeptidase that has been identified as one of the major proteins of the GLUT4-containing vesicles. In the present study we have determined the degree of co-localization between vp165 and GLUT4 in rat adipose cells and used perturbation by wortmannin to assess the exocytic and endocytic steps...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Malide D,St-Denis JF,Keller SR,Cushman SW

    更新日期:1997-06-16 00:00:00

  • Insertion of foreign random sequences of 120 amino acid residues into an active enzyme.

    abstract::Random sequences of 120-130 amino acid residues were inserted into a surface loop region of Escherichia coli RNase HI. This library was screened and about 10% of the clones were found to retain RNase H activity. Subsequent random mutagenesis led to an increase in RNase H activity and solubility of the protein. The ins...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Doi N,Itaya M,Yomo T,Tokura S,Yanagawa H

    更新日期:1997-02-03 00:00:00

  • Three different GB virus C/hepatitis G virus genotypes. Phylogenetic analysis and a genotyping assay based on restriction fragment length polymorphism.

    abstract::The 5'-untranslated region (5'-UTR) sequences of 33 GB virus C/hepatitis G virus (GBV-C/HGV) obtained from different geographic areas were determined through reverse-transcription polymerase chain reaction and dideoxy chain termination sequencing, the alignment of sequences, the estimation of the number of nucleotide ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mukaide M,Mizokami M,Orito E,Ohba K,Nakano T,Ueda R,Hikiji K,Iino S,Shapiro S,Lahat N,Park YM,Kim BS,Oyunsuren T,Rezieg M,Al-Ahdal MN,Lau JY

    更新日期:1997-04-21 00:00:00

  • Correlation between phospholipase A2 activity and intra-Golgi protein transport reconstituted in a cell-free system.

    abstract::A wide variety of phospholipase A2 inhibitors blocks intra-Golgi protein transport reconstituted in a cell-free system. Phospholipase A2 activity detectable under the protein transport assay conditions is actually inhibited by the inhibitors. There is a good correlation between the inhibition of protein transport and ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tagaya M,Henomatsu N,Yoshimori T,Yamamoto A,Tashiro Y,Fukui T

    更新日期:1993-06-14 00:00:00

  • Tissue-specific regulation of the expression of rat intestinal bile acid-binding protein.

    abstract::A lipid-binding protein identical to the rat intestinal bile acid-binding protein, termed I-15P, was expressed in steroid hormone-producing tissues such as ovary and adrenal gland, but not testis. In immature rats, I-15P was expressed in intestine but not in ovaries. The expression of I-15P in the ovaries of immature ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sato E,Fujii H,Fujita M,Kanda T,Iseki S,Hatakeyama K,Tanaka T,Ono T

    更新日期:1995-10-30 00:00:00

  • Nucleolar disruption impairs Kaposi's sarcoma-associated herpesvirus ORF57-mediated nuclear export of intronless viral mRNAs.

    abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) ORF57 plays a pivotal role in mediating the nuclear export of KSHV intronless transcripts. We demonstrate that ORF57 localises and dynamically trafficks through the nucleolus. To assess if nucleolar shuttling of ORF57 is important for the export of intronless viral mRNA, ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Boyne JR,Whitehouse A

    更新日期:2009-11-19 00:00:00

  • Revisiting the scissor-like mechanism of activation for the erythropoietin receptor.

    abstract::An interpretation of alternative crystal structures of the erythropoietin receptor, with and without ligand, led to the proposal of a scissor-like mechanism of activation. This model has been propagated in the literature and is still being used to interpret crystal structures of related type-I cytokine receptors. Here...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Corbett MS,Poger D,Mark AE

    更新日期:2016-09-01 00:00:00

  • APP RNA splicing is not affected by differentiation of neurons and glia in culture.

    abstract::Amyloid precursor protein (APP) gene expression was investigated in primary cultures of neurons, astrocytes, microglial cells and oligodendrocytes. Neurons from various rat brain regions, as well as oligodendrocytes, contained RNA encoding APP695, while astrocytes and microglial cells expressed high levels of RNAs for...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gaul G,Dutly F,Frei K,Foguet M,Lübbert H

    更新日期:1992-08-03 00:00:00

  • Use of monoclonal antibodies to analyse the expression of a multi-tubulin family.

    abstract::We have used a panel of monoclonal antibodies in a study of the expression of multiple tubulins in Physarum polycephalum. Three anti-beta-tubulin monoclonal antibodies, DM1B, DM3B3 and KMX-1 all reacted with the beta 1-tubulin isotypes expressed in both myxamoebae and plasmodia. However, these antibodies showed a spec...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Birkett CR,Foster KE,Johnson L,Gull K

    更新日期:1985-08-05 00:00:00

  • Insulin-like growth factor I supports differentiation of cultured osteoblast-like cells.

    abstract::Rat calvaria cells grown in culture for one week had properties of osteoblasts: a high content in alkaline phosphatase and a marked cyclic AMP response to parathyroid hormone (PTH). In short-term experiments, insulin-like growth factor I (IGF I) stimulated the incorporation of [14C] glucose into glycogen. When IGF I w...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schmid C,Steiner T,Froesch ER

    更新日期:1984-07-23 00:00:00

  • isomiRex: web-based identification of microRNAs, isomiR variations and differential expression using next-generation sequencing datasets.

    abstract::We present an open-access web platform isomiRex, to identify isomiRs and on the fly graphical visualization of the differentially expressed miRNAs in control as well as treated library. The open-access web-platform is not restricted only to NGS sequence dataset from animals and potentially analyzes a wider dataset for...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sablok G,Milev I,Minkov G,Minkov I,Varotto C,Yahubyan G,Baev V

    更新日期:2013-08-19 00:00:00

  • Proteinases of betaretroviruses bind single-stranded nucleic acids through a novel interaction module, the G-patch.

    abstract::Retroviral proteinases (PRs) are essential for retrovirus infectivity but the mechanism of their activity regulation is poorly understood. We investigated possible involvement in this process of the C-terminal domain (CTD) of betaretroviral PRs. We found that the presence of CTD attenuates proteolytic activity of Maso...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Svec M,Bauerová H,Pichová I,Konvalinka J,Strísovský K

    更新日期:2004-10-08 00:00:00

  • A single d(GpG) cisplatin adduct on the estrogen response element decreases the binding of the estrogen receptor.

    abstract::Both cisplatin and the estrogen receptor (ER) are known to bend DNA. The influence of the bending of sequences by the d(GpG)cisPt adduct binding of ER to estrogen response element (ERE)-like sequences was examined. Three ERE-like oligonucleotides with different affinities for ER and which include a GG in the linker se...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Massaad-Massade L,Massaad C,Legendre F,Bas V,Chottard J,Beaune P,Barouki R

    更新日期:2000-01-21 00:00:00

  • Liposome destabilization induced by the HIV-1 fusion peptide effect of a single amino acid substitution.

    abstract::The 23-residue synthetic peptide representing the N-terminus of HIV-1 gp41 is known to induce either leakage or fusion of lipid vesicles depending on the experimental conditions. In this paper we report that a polar amino acid substitution V-->E at position 2, known to block gp41 activity in vivo, makes the peptide un...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pereira FB,Goñi FM,Nieva JL

    更新日期:1995-04-03 00:00:00

  • Peptidyl-prolyl cis-trans isomerase activity as studied by dynamic proton NMR spectroscopy.

    abstract::Recently the identity of the peptidyl-prolyl cis-trans isomerase (PPIase), which accelerates the cis/trans isomerization of prolyl peptide bonds and cyclophilin, the binding protein for the immunosuppressive drug Cyclosporin A (CsA), was discovered. The PPIase catalysis toward the substrate Suc-Ala-Phe-Pro-Phe-pNA has...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hübner D,Drakenberg T,Forsén S,Fischer G

    更新日期:1991-06-17 00:00:00

  • Histone H5 and H1 cross-reacting material is restricted to erythroid cells in chicken.

    abstract::Monoclonal H5 antibodies and a polyclonal antiserum, raised against the globular domain of chicken H5 (GH5) but which cross-reacts with histone H1(0) from mouse liver, were used to search for H5 or H1 (0)-like proteins in chicken embryo and adult tissue sections by indirect immunofluorescence. Chicken cell lines in cu...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Shannon MF,Wigley PL,Wells JR

    更新日期:1985-07-08 00:00:00

  • Molecular cloning of hemocyanin cDNA from Penaeus vannamei (Crustacea, Decapoda): structure, evolution and physiological aspects.

    abstract::Hemocyanin is present as 2 subunits in the hemolymph of Penaeus vannamei. Isolated from a hepatopancreas cDNA library of this penaeid shrimp, the cDNA chain (2095 bp) corresponds to a full length hemocyanin messenger as determined by Northern hybridization, with a short 5' untranslated region (17 bp), an open reading ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sellos D,Lemoine S,Van Wormhoudt A

    更新日期:1997-04-28 00:00:00

  • MiR-199a-3p promotes gastric cancer progression by targeting ZHX1.

    abstract::Accumulating evidence has indicated that microRNAs (miRNAs) act as critical epigenetic regulators in tumor carcinogenesis. Here, we report that miR-199a-3p was significantly upregulated in gastric cancer (GC) cell lines and tissues. Functional studies demonstrated that miR-199a-3p dramatically increased cell prolifera...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wang Z,Ma X,Cai Q,Wang X,Yu B,Cai Q,liu B,Zhu Z,Li C

    更新日期:2014-11-28 00:00:00

  • FKBP25, a novel regulator of the p53 pathway, induces the degradation of MDM2 and activation of p53.

    abstract::The p53 tumour suppressor protein is tightly controlled by the E3 ubiquitin ligase, mouse double minute 2 (MDM2), but maintains MDM2 expression as part of a negative feedback loop. We have identified the immunophilin, 25kDa FK506-binding protein (FKBP25), previously shown to be regulated by p53-mediated repression, as...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ochocka AM,Kampanis P,Nicol S,Allende-Vega N,Cox M,Marcar L,Milne D,Fuller-Pace F,Meek D

    更新日期:2009-02-18 00:00:00

  • Two-dimensional gel electrophoresis and FTIR spectroscopy reveal both forms of yeast plasma membrane H(+)-ATPase in activated and basal-level enzyme preparations.

    abstract::Plasma membrane H(+)-ATPase of the yeast Saccharomyces cerevisiae was isolated and purified in its two forms, the activated A-ATPase from glucose-metabolizing cells, and the basal-level B-ATPase from cells with endogenous metabolism only. Using two-dimensional gel electrophoretic analysis, we showed that both enzyme p...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lapathitis G,Tanfani F,Kotyk A,Bertoli E

    更新日期:2001-09-07 00:00:00

  • Rotational dynamics of 4-aminobutyrate aminotransferase.

    abstract::The fluorescence dye 1-anilinonaphthalene-8-sulfonate (ANS) was used as a probe of non-polar binding sites in 4-aminobutyrate aminotransferase. ANS binds to a single binding site of the dimeric protein with a Kd of 6 microM. Nanosecond emission anisotropy measurements were performed on the ANS-enzyme in an effort to d...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Churchich JE,Kim DS,Schnackerz KD

    更新日期:1983-08-22 00:00:00

  • The 49-kDa subunit of NADH-ubiquinone oxidoreductase (Complex I) is involved in the binding of piericidin and rotenone, two quinone-related inhibitors.

    abstract::Piericidin is a potent inhibitor of the mitochondrial and bacterial type I NADH-ubiquinone oxidoreductases (Complex I) and is considered to bind at or close to the ubiquinone binding site(s) of the enzyme. Piericidin-resistant mutants of the bacterium Rhodobacter capsulatus have been isolated and the present work demo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Darrouzet E,Issartel JP,Lunardi J,Dupuis A

    更新日期:1998-07-10 00:00:00

  • Cloning and expression of the epsilon 4 subunit of the NMDA receptor channel.

    abstract::The primary structure of a novel subunit of the mouse NMDA (N-methyl-D-aspartate) receptor channel, designated epsilon 4, has been revealed by cloning and sequencing the cDNA. The epsilon 4 subunit shares high amino acid sequence identity with the epsilon 1, epsilon 2 and epsilon 3 subunits of the mouse NMDA receptor ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ikeda K,Nagasawa M,Mori H,Araki K,Sakimura K,Watanabe M,Inoue Y,Mishina M

    更新日期:1992-11-16 00:00:00

  • Psoralen photofootprinting of protein-binding sites on DNA.

    abstract::Using a BAL31 exonuclease assay to determine the sites of 4,5',8-trimethylpsoralen photocrosslinking in DNA we have shown that 5'-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, lambda-repressor E. coli or RNA polymerase are bound at t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zhen WP,Jeppesen C,Nielsen PE

    更新日期:1988-02-29 00:00:00

  • Specific binding of phenolic glycolipid antigens from Mycobacterium bovis BCG with antibodies.

    abstract::We studied the molecular binding specificity of two rabbit polyclonal sera generated against phenolic glycolipid antigens namely PheG1 B and PheG1 B-3 from Mycobacterium bovis BCG. PheG1 B is the well-known mycoside B (2-O-Me-alpha-L-Rhap 1----aglycone), while PheG1 B-3 is a recently found glycolipid (alpha-L-Rhap-(1-...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vercellone A,Riviere M,Fournié JJ,Puzo G

    更新日期:1992-05-25 00:00:00

  • Postnatal developmental profiles of filamentous actin and of 200 kDa neurofilament polypeptide in the visual cortex of light- and dark-reared rats and their relationship to critical period plasticity.

    abstract::The concept of critical period plasticity in rat visual cortex has been studied in terms of changes in the level of filamentous actin and of the 200 kDa neurofilament polypeptide. Our results suggest that the postnatal developmental profile of filamentous actin is affected by visual experience, as a consequence of eye...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nona SN,Trowell SC,Cronly-Dillon JR

    更新日期:1985-07-01 00:00:00

  • Structural modeling of dual-affinity purified Pho84 phosphate transporter.

    abstract::The phosphate transporter Pho84 of Saccharomyces cerevisiae is predicted to contain 12 transmembrane (TM) regions, divided into two partially duplicated parts of 6 TM segments. The three-dimensional (3D) organization of the Pho84 protein has not yet been determined. However, the 3D crystal structure of the Escherichia...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lagerstedt JO,Voss JC,Wieslander A,Persson BL

    更新日期:2004-12-17 00:00:00

  • Proton transfer in uncoupled variants of cytochrome c oxidase.

    abstract::Cytochrome c oxidase is a membrane-bound redox-driven proton pump that harbors two proton-transfer pathways, D and K, which are used at different stages of the reaction cycle. Here, we address the question if a D pathway with a modified energy landscape for proton transfer could take over the role of the K pathway whe...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vilhjálmsdóttir J,Albertsson I,Blomberg MRA,Ädelroth P,Brzezinski P

    更新日期:2020-03-01 00:00:00

  • A protein produced by a monocytic human cell line can induce apoptosis on tumor cells.

    abstract::A serum-free medium conditioned by U937, a human cell line of monocyte/macrophage origin, was found capable of inducing apoptosis on exponentially growing U937 cells themselves (autocrine suicide). The apoptosis-inducing agent is a macromolecule and possibly a protein (SKT factor), with a relative molecular mass in th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ghibelli L,Coppola S,Nosseri C,Bergamini A,Beninati S

    更新日期:1994-05-09 00:00:00

  • Clear cell carcinoma of the human ovary synthesizes and secretes a transferrin with microheterogeneity of lectin affinity.

    abstract::Human ovarian clear cell carcinoma cell line (transferrin (Tf)-non-producer), HAC 2, cells were adapted to grow in chemically defined synthetic medium when the cells were cultured with medium containing 10 micrograms/ml of insulin at least for 6 months. They synthesized and secreted constantly the 80 kDa protein immun...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ohkawa K,Takada K,Takizawa N,Hatano T,Tsukada Y,Matsuda M

    更新日期:1990-09-17 00:00:00