Abstract:
:In this study we used the fungal antibiotic brefeldin A (BFA) to analyze its effect on viral replication. Analysis by electron microscopy demonstrated that no viral particles were observed in cells treated before the onset of viral replication. In the presence of BFA expression of IE2, MCP, pUL104, pUL56 and pUL89 were reduced, while no or slight effect was observed on expression of pp65, pUL44 and pUL57. Strikingly, real time PCR revealed that de novo viral DNA synthesis is reduced but not completely abolished in the presence of BFA. These results indicated that BFA represents a multi-functional compound leading to inhibition of several steps of viral maturation such as expression of viral DNA packaging proteins and capsid formation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kregler O,Schilf R,Lander A,Bannert N,Bogner Edoi
10.1016/j.febslet.2009.03.011subject
Has Abstractpub_date
2009-04-02 00:00:00pages
1207-14issue
7eissn
0014-5793issn
1873-3468pii
S0014-5793(09)00190-2journal_volume
583pub_type
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