Abstract:
:DNA polymerase alpha-primase is a heterotetrameric complex essential for simian vacuolating virus 40 (SV40) DNA replication. We show that the C-terminal 67 amino acid residues of the human p180 subunit are essential for SV40 DNA replication as they are required for binding of the p68 subunit and play a role in the interaction with the primase subunits, p48 and p58. Furthermore, we demonstrate that exchanging these residues to those of mouse origin can only partially rescue the SV40 DNA replication activity of DNA polymerase alpha-primase.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Smith RW,Nasheuer HPdoi
10.1016/s0014-5793(02)03197-6keywords:
subject
Has Abstractpub_date
2002-09-11 00:00:00pages
143-6issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579302031976journal_volume
527pub_type
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