Abstract:
:We have recently cloned a cDNA encoding sucrose:fructan 6-fructosyltransferase (6-SFT), a key enzyme of fructan synthesis forming the beta-2,6 linkages typical of the grass fructans, graminans and phleins [Sprenger et al. (1995) Proc. Natl. Acad. Sci. USA 92, 11652-11656]. Here we report functional expression of 6-SFT from barley in transgenic tobacco and chicory. Transformants of tobacco, a plant naturally unable to form fructans, synthesized the trisaccharide kestose and a series of unbranched fructans of the phlein type (beta-2,6 linkages). Transformants of chicory, a plant naturally producing only unbranched fructans of the inulin type (beta-2,1 linkages), synthesized in addition branched fructans of the graminan type, particularly the tetrasaccharide bifurcose which is also a main fructan in barley leaves.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sprenger N,Schellenbaum L,van Dun K,Boller T,Wiemken Adoi
10.1016/s0014-5793(96)01418-4subject
Has Abstractpub_date
1997-01-06 00:00:00pages
355-8issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(96)01418-4journal_volume
400pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Annexin I (AnxI) is a calcium-dependent membrane binding protein which has been implicated in various physiological activities. The region of the chicken anxI cDNA encoding the first 130 amino terminal residues was cloned by reverse transcription PCR in order to determine the relationship of its variable amino-termina...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80241-l
更新日期:1993-08-30 00:00:00
abstract::The number of synthetic UTP analogues containing methyl groups in different positions of the ribose moiety were tested as substrates for T7 RNA polymerase (T7 RNAP). Two of these compounds (containing substituents in the 5' position) were shown to be weak substrates of T7 RNAP. 3'Me-UTP was neither substrate nor inhib...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01400-7
更新日期:1997-01-06 00:00:00
abstract::Particulate drug carriers, including liposomes, are rapidly removed from blood by cells of the reticuloendothelial system (RES) with resulting adverse effects on this important host defense system. In order to overcome this and other major disadvantages of liposomes, we have altered liposome composition in an effort t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80506-9
更新日期:1987-10-19 00:00:00
abstract::Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin affinity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identified by mass spectrometry as adenosine kinase. The ligand interaction reported here can be dis...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03750-x
更新日期:2003-01-02 00:00:00
abstract::The 4-chlorobenzoyl-CoA dehalogenase catalyzes the hydrolytic dechlorination of 4-chlorobenzoyl-CoA via a two-step mechanism, namely nucleophilic aromatic substitution and ester hydrolysis. The mutation of an active-site Histidine residue has been shown to reduce the catalytic activity in both the substitution and sub...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.06.056
更新日期:2005-08-15 00:00:00
abstract::The importance of the individual amino acid residues of AIP (KKALRRQEAVDAL), a highly specific inhibitor of calmodulin-dependent protein kinase II (CaMKII), was studied. Replacement of Arg6, Gln7, or Ala9 by other amino acid residues produced a marked increase in the IC50 value. Leu4 and Val10 were also sensitive to r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00405-0
更新日期:1998-05-01 00:00:00
abstract::The contribution of hepatic glycogen to lipogenesis was studied in isolated, intact rat hepatocytes. To establish its importance as a substrate for lipogenesis, the glycogen of isolated hepatocytes was prelabelled with 14C from glucose. Evidence is presented that neither glucose nor glycogen constitute major sources o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80558-x
更新日期:1984-09-17 00:00:00
abstract::The fluorescence intensity of the Ca2+,Mg2(+)-ATPase protein of rabbit skeletal sarcoplasmic reticulum that incorporated about 2 mol of 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) was enhanced at high MgATP concentrations with or without 50 microM calcium. The observed enhancement indicates that the fluorophore, ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80021-a
更新日期:1990-01-01 00:00:00
abstract::hTrp3 is a human homologue of the Drosophila gene responsible for a transient receptor potential (trp) mutation. When stably expressed in HEK293 cells, hTrp3 formed ion channels that were active under resting conditions but could be further stimulated by carbachol or ATP via endogenous muscarinic or purinergic recepto...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00035-0
更新日期:1998-02-06 00:00:00
abstract::Di(1,N6-ethenoadenosine)5',5'''-P1,P4-tetraphosphate, epsilon-(Ap4A), a fluorescent analog of Ap4A has been synthesized by reaction of 2-chloroacetaldehyde with Ap4A. At neutral pH this Ap4A analog presents characteristics maxima at 265 and 274 nm, shoulders at ca 260 and 310 nm and moderate fluorescence (lambda exc 3...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80334-y
更新日期:1991-03-25 00:00:00
abstract::Piericidin is a potent inhibitor of the mitochondrial and bacterial type I NADH-ubiquinone oxidoreductases (Complex I) and is considered to bind at or close to the ubiquinone binding site(s) of the enzyme. Piericidin-resistant mutants of the bacterium Rhodobacter capsulatus have been isolated and the present work demo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00719-4
更新日期:1998-07-10 00:00:00
abstract::Decades of neuroscience research have shed light on the hippocampus as a key structure for the formation of episodic memory. The hippocampus is divided into distinct subfields - CA1, CA2 and CA3. While accumulating evidence points to cellular and synaptic heterogeneity within each subfield, this heterogeneity has not ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2014.06.004
更新日期:2014-08-01 00:00:00
abstract::Epigallocatechin-3-gallate (EGCG) is a major component of green tea polyphenols which displays potential properties of anticancer and neuroprotection. Here we show that protection of motor neuron by EGCG is associated with regulating glutamate level in organotypic culture of rat spinal cord. In this model, EGCG blocke...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.05.011
更新日期:2010-07-02 00:00:00
abstract::The effect of the uremic solute indoxyl sulfate (IS) on scavenging superoxide anion radicals (O(2)(*-)) generated from both the xanthine/xanthine oxidase (X/XO) system and activated neutrophils was investigated by electron paramagnetic resonance spectroscopy, combined with 2-ethoxycarbonyl-2-methyl-3,4-dihydro-2H-pyrr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.04.046
更新日期:2010-07-02 00:00:00
abstract::Epidemiological studies testing the effect of beta-carotene in humans have found a relative risk for lung cancer in smokers supplemented with beta-carotene. We investigated the reactions of retinal and beta-apo-8'-carotenal, two beta-carotene oxidation products, with 2'-deoxyguanosine to evaluate their DNA damaging po...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(04)00084-5
更新日期:2004-02-27 00:00:00
abstract::Diphtheria toxin fragment A is able to inhibit protein synthesis in the eukaryotic cell by ADP-ribosylating the diphthamide residue of elongation factor-2 (EF-2) [(1980) J. Biol. Chem. 255, 10710-10720]. The reaction requires NAD as ADP-ribose donor. This work reports on the capacity of an NAD analog, the nicotinamide...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80006-5
更新日期:1985-10-28 00:00:00
abstract::There is evidence that CCK-8 may interact with opioids and that both systems are probably implicated in pain modulation. In order to elucidate this relationship we sought to examine factors governing the movement of CCK-8 from the spinal cord into the extracellular space. We report that CCK-8 like immunoreactivity, as...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80723-9
更新日期:1989-07-03 00:00:00
abstract::Rat soleus muscle strips cultured for 24 h in medium 199 were well preserved in terms of electron microscopy; ATP and creatine phosphate concentrations; rates of glucose utilization, glycogen and protein synthesis, and effects of insulin thereon. Culture led to modest changes in fluid spaces and intracellular (K+); in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81058-v
更新日期:1990-10-29 00:00:00
abstract::Mouse embryonic stem (ES) cells homozygous for disruption of the MSK1 gene had no detectable MSK1 activity. However, their activators (extracellular signal related kinase (ERK)1/ERK2) were stimulated normally in mitogen- and stress-activated protein kinase (MSK)1-/- and wild type cells in response to tetradecanoylphor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02031-7
更新日期:2000-09-29 00:00:00
abstract::Regulated permeability changes have been detected in mitochondria across species. We review here their key features, with the goal of assessing whether a "permeability transition" similar to that observed in higher eukaryotes is present in other species. The recent discoveries (i) that treatment with cyclosporin A (Cs...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.04.023
更新日期:2010-06-18 00:00:00
abstract::The WW domain is known as one of the smallest protein modules with a triple-stranded beta-sheet fold. Here, we present the solution structure of the second WW domain from the mouse salvador homolog 1 protein. This WW domain forms a homodimer with a beta-clam-like motif, as evidenced by size exclusion chromatography, a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.01.008
更新日期:2007-02-06 00:00:00
abstract::The thermal blockage of QA-. oxidation was analysed in PS II membrane fragments by monitoring flash-induced changes of the relative fluorescence quantum yield as a function of temperature. The results obtained reveal: (a) in dark-adapted samples the fraction of QA-. that is not reoxidised within a time domain of 10 s ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00491-8
更新日期:1998-05-29 00:00:00
abstract::Pin2/TRF1 was independently identified as a telomeric DNA-binding protein (TRF1) that regulates telomere length, and as a protein (Pin2) that can bind the mitotic kinase NIMA and suppress its lethal phenotype. We have previously demonstrated that Pin2/TRF1 levels are cell cycle-regulated and its overexpression induces...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02363-3
更新日期:2002-03-13 00:00:00
abstract::A major house dust mite allergen Der f 1 belongs to the papain-like cysteine protease family. This study investigated whether Der f 1 can cleave the latency-associated peptide (LAP) of transforming growth factor (TGF)-beta via its proteolytic activity and activate latent TGF-beta. We found that Der f 1 can cleave LAP ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.05.030
更新日期:2009-06-18 00:00:00
abstract::We found that, in the presence of chimeric oligonucleotides containing complementary deoxyribo- and 2'-O-methylnucleosides, a nonaribonucleotide, [5'-32P]pACUUACCUG, was cleaved specifically upon treatment with RNase H. When 3'm(UG)d(AATG)m(GAC)5' was used as a hybridization strand, pACUUACCUG was cleaved between C6 a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80171-0
更新日期:1987-05-11 00:00:00
abstract::Uncoupling protein 3 (UCP3) may reduce mitochondrial ROS production, and thereby protect against mitochondrial dysfunction in skeletal muscle. UCP3 has been suggested to specifically fulfill this role under high-fat conditions. Here we show that UCP3 knockout mice indeed have elevated mitochondrial ROS production afte...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.11.012
更新日期:2011-12-15 00:00:00
abstract::Human synovium was analyzed for the possible expression of cartilage oligomeric matrix protein (COMP). Immunostaining with polyclonal antiserum to COMP demonstrated positive staining within the synovial cells and immediately subjacent connective tissue, with less intense staining in the deeper connective tissue. Weste...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00789-8
更新日期:1997-07-21 00:00:00
abstract::Winter flounder contains two distinct anti-freeze protein isoforms, which are the liver-type extracellular anti-freeze proteins and the skin-type intracellular anti-freeze protein. The skin-type anti-freeze proteins exhibit lower anti-freeze activities than the liver-type isoforms and this might be due to their lackin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00752-8
更新日期:1999-06-25 00:00:00
abstract::The N-terminus of MDM2 proto-oncoprotein interacts with p53 and down modulates p53 activity by inhibiting transcriptional activity and promoting p53 degradation. MDMX is structurally related to MDM2 and also binds to p53. However, the function of MDMX has not been clarified yet. We found that MDM2 hetero-oligomerized ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00254-9
更新日期:1999-03-19 00:00:00
abstract::The isolation and primary structure of intestinal neuropeptide Y (NPY) is described. The peptide was purified from porcine intestinal extracts using a chemical assay and radioimmunoassay for NPY. The amino acid sequence of this peptide is: Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu-Asp-Ala-Pro-Ala-Glu-Asp-Leu-Ala- Arg-Ty...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80216-7
更新日期:1985-01-01 00:00:00