Abstract:
:We found that, in the presence of chimeric oligonucleotides containing complementary deoxyribo- and 2'-O-methylnucleosides, a nonaribonucleotide, [5'-32P]pACUUACCUG, was cleaved specifically upon treatment with RNase H. When 3'm(UG)d(AATG)m(GAC)5' was used as a hybridization strand, pACUUACCUG was cleaved between C6 and C7 to yield pACUUAC. In the presence of 3'm(UGAA)d(TGGA)m(C)5', the nonaribonucleotide was hydrolyzed, mainly between U8 and C9, to give pACUUACCU. This method will have a variety of applications in the field of RNA engineering.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Inoue H,Hayase Y,Iwai S,Ohtsuka Edoi
10.1016/0014-5793(87)80171-0subject
Has Abstractpub_date
1987-05-11 00:00:00pages
327-30issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(87)80171-0journal_volume
215pub_type
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