Abstract:
:Chemical conjugation of fatty acids to antibodies generates lipid-modified molecules which have found use in the targeting of liposome-mediated drug delivery and in liposome-based immunoassays. Alternatively, bacterial expression of antibodies as single-chain FV fragments fused to lipoprotein signal peptide and N-terminal sequence leads to in vivo enzymatic addition of a single glycerolipid group at the N-terminus of the molecule. This lipid-modification converts the antibody from a soluble protein into a functional membrane-bound molecule. These biosynthetically lipid-tagged antibodies may prove useful for immobilization of antibodies to membranes in various biotechnological applications.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Keinänen K,Laukkanen MLdoi
10.1016/0014-5793(94)00313-0subject
Has Abstractpub_date
1994-06-06 00:00:00pages
123-6issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(94)00313-0journal_volume
346pub_type
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