Saturable [D-Ala2, D-Leu5]-enkephalin transport into cholinergic synaptic vesicles.

abstract：:Biochemical, immunohistochemical and molecular biological methods were used to detect fetal myosin heavy chain (MyHC) in the human masseter of elderly and young subjects. Samples from the elderly subjects contained larger amounts of fetal MyHC than those of young adults. Only a very small amount of embryonic MyHC coul...

journal_title：FEBS letters

authors： Monemi M,Eriksson PO,Dubail I,Butler-Browne GS,Thornell LE

更新日期：1996-05-13 00:00:00

abstract：:The mechanism of stimulation of hepatic respiration by fatty acids was studied in isolated rat hepatocytes. Stimulation of respiration by fatty acids varied from about 35% to about 105% depending on chain length. The stimulatory effect of octanoate (1 mM) or oleate (0.5 mM) was prevented by oligomycin (2 micrograms/ml...

journal_title：FEBS letters

authors： Plomp PJ,van Roermund CW,Groen AK,Meijer AJ,Tager JM

更新日期：1985-12-02 00:00:00

abstract：:Treatment of yeast phosphoglycerate kinase (PGK) with trypsin results in a fourfold increase in the Vmax of this enzyme, without affecting the Km. This activation is shown to be due to the removal of the C-terminal lysine residue. The C-terminal sequence folds back over the N-terminal domain and contacts the extreme N...

journal_title：FEBS letters

authors： Adams B,Fowler R,Hudson M,Pain RH

更新日期：1996-04-29 00:00:00

abstract：:Horse heart cytochrome c is cleaved by thermolysin in 50% aqueous TFE (v/v) at neutral pH (25 degrees C, 24 h) at the Gly56-Ile57 peptide bond of the 104-residue chain of the protein. Additional, but anyway minor, fragmentation at the Gly45-Phe46 and Met80-Ile81 peptide bonds is also observed. On the other hand, in bu...

journal_title：FEBS letters

authors： Fontana A,Zambonin M,De Filippis V,Bosco M,Polverino de Laureto P

更新日期：1995-04-10 00:00:00

abstract：:The OxyR protein is a transcriptional activator for a subset of peroxide stress-inducible genes, most of which are involved in defense systems against oxidative stress. Recently, it was demonstrated that purified OxyR has one intramolecular disulfide bond, which led to the proposal that the reversible disulfide bond f...

journal_title：FEBS letters

authors： Tao K

更新日期：1999-08-20 00:00:00

abstract：:Two lipophilic derivatives of caffeic acid which inhibit lipoxygenase, caffeic acid phenethyl ester (CAPE) and N,N'-dicyclohexyl-O-(3,4-dihydroxycinnamoyl)-isourea (DCHCU), reduced the proliferative response of murine splenocytes to concanavalin A in vitro. Both CAPE and DCHCU induced apoptosis in murine thymocyte cul...

journal_title：FEBS letters

authors： Mirzoeva OK,Yaqoob P,Knox KA,Calder PC

更新日期：1996-11-04 00:00:00

abstract：:There are numerous examples of how fundamental research has been required to understand and treat human disease. This article focuses on three human diseases of lipid metabolism in which advancements in understanding and treatment would not have been possible without basic research. Fabry disease is an inherited metab...

journal_title：FEBS letters

authors： Vance DE

更新日期：2006-10-09 00:00:00

abstract：:Under hypobaric hypoxia, antioxidant defenses of the heart are stressed by the enhanced production of ROS. Mammalian heart acclimatizes to hypoxia through altered gene expression, which we studied in murine heart exposed to 10h of acute hypobaric hypoxia (AHH), equivalent to 15000ft, using cDNA arrays. Functional clas...

journal_title：FEBS letters

authors： Karar J,Dolt KS,Mishra MK,Arif E,Javed S,Pasha MA

更新日期：2007-10-02 00:00:00

abstract：:To identify suitable promoters for expressing exogenous genes in arthritic joints, the constitutive, simian virus 40 (SV 40) and IL-1 or metal inducible, human stromelysin and metallothionein (MT) gene promoters were tested for their activity in chondrocytes and synovial fibroblasts. Transient transfection with plasmi...

journal_title：FEBS letters

authors： Su S,Gedamu L,Dehnade F,Zafarullah M

更新日期：1996-10-21 00:00:00

abstract：:Protein deglutathionylation is mainly catalyzed by glutaredoxins (GRXs). We have analyzed the biochemical properties of four of the six different GRXs of Chlamydomonas reinhardtii. Kinetic parameters were determined for disulfide and dehydroascorbate reduction but also for deglutathionylation of artificial and protein...

journal_title：FEBS letters

authors： Gao XH,Zaffagnini M,Bedhomme M,Michelet L,Cassier-Chauvat C,Decottignies P,Lemaire SD

更新日期：2010-06-03 00:00:00

abstract：:We have investigated the functional importance of nucleotide sequence differences between proximal (P-) and distal (D-) p53-binding elements in the MCK promoter. P- and D-elements normally co-operate to permit synergistic promoter activation by p53. Interestingly, we find that P-elements cannot co-operate with each ot...

journal_title：FEBS letters

authors： Jackson P,Yardley G

更新日期：1997-04-14 00:00:00

abstract：:Insulin receptors (IRs) that are truncated at the end of the ectodomain form dimers that bind insulin with different characteristics to wild type receptors. These soluble IRs have lowered affinity for insulin compared with full-length IR, and exhibit linear Scatchard plots in contrast to the curvilinear plots obtained...

journal_title：FEBS letters

authors： Hoyne PA,Cosgrove LJ,McKern NM,Bentley JD,Ivancic N,Elleman TC,Ward CW

更新日期：2000-08-11 00:00:00

abstract：:Based on imino proton exchange catalysis, base-pair lifetimes and apparent dissociation constants are reported on the complexes formed by bisintercalation of echinomycin at the CpG steps of the d(ACGTATACGT)2 and d(ACGTTAACGT)2 duplexes. The lifetimes of the four central A x T base pairs between two echinomycin bindin...

journal_title：FEBS letters

authors： Park JY,Lee JH,Choi BS

更新日期：1998-04-24 00:00:00

abstract：:We have used the polymerase chain reaction (PCR) to synthesise a cDNA encoding part of human cardiac troponin I. Amplification was achieved using fully degenerate sets of oligonucleotides corresponding to conserved regions of amino acid sequence identified in other troponin I isoforms. The cloned PCR fragment was subs...

journal_title：FEBS letters

authors： Vallins WJ,Brand NJ,Dabhade N,Butler-Browne G,Yacoub MH,Barton PJ

更新日期：1990-09-17 00:00:00

abstract：:Astrocytes (AC) induce blood-brain barrier (BBB) properties in brain endothelial cells (EC). As antioxidative activity (AOA) is assumed to be a BBB characteristic, we tested whether AC improve AOA of EC. Monocultivated AC showed higher AOA [manganese superoxide dismutase (SOD), catalase (Cat), glutathione peroxidase (...

journal_title：FEBS letters

authors： Schroeter ML,Mertsch K,Giese H,Müller S,Sporbert A,Hickel B,Blasig IE

更新日期：1999-04-23 00:00:00

abstract：:Activation of protein kinase C (PKC) in Rat-1 fibroblasts leads to rapid phosphorylation of an 80-kDa protein, a major substrate of PKC. Digitonin-permeabilized cells perfectly supported this early response. Introduction of a PKC pseudosubstrate peptide inhibited 80 kDa phosphorylation with an IC50 of 1 microM, while ...

journal_title：FEBS letters

authors： Eichholtz T,Alblas J,van Overveld M,Moolenaar W,Ploegh H

更新日期：1990-02-12 00:00:00

abstract：:Irreversible conversion of the purified zinc-containing 7Fe ferredoxin from the thermoacidophilic archaeon Sulfolobus sp. strain 7 was found to occur under aerobic conditions at pH 5.0 and at 4 degrees C. This process accompanied a substantial increase of the electron paramagnetic resonance signal attributed to a [3Fe...

journal_title：FEBS letters

authors： Iwasaki T,Oshima T

更新日期：1997-11-10 00:00:00

abstract：:A photoactive reaction center complex was isolated from a thermophilic green sulfur bacterium, Chlorobium tepidum under anaerobic conditions. The electron transfer occurred from heme c to the photo-oxidized reaction center chlorophyll, P840+, with a half time (t1/2) of 110 or 340 microseconds at 24 or 12 degrees C, re...

journal_title：FEBS letters

authors： Oh-oka H,Kamei S,Matsubara H,Iwaki M,Itoh S

更新日期：1995-05-22 00:00:00

abstract：:Glutathione peroxidase 3 (GPX3) is an important member of antioxidant enzymes for reducing reactive oxygen species and maintaining the oxygen balance. Gpx3 mRNA is strongly expressed in decidual cells from days 5 to 8 of pregnancy. After pregnant mice are treated with GPX inhibitor for 3 days, pregnancy rate is signif...

journal_title：FEBS letters

authors： Xu X,Leng JY,Gao F,Zhao ZA,Deng WB,Liang XH,Zhang YJ,Zhang ZR,Li M,Sha AG,Yang ZM

更新日期：2014-05-02 00:00:00

abstract：:Adrenomedullin (ADM) functions as a survival factor against hypoxic cell death. However, molecular mechanisms underlying the cell survival pathway remain largely unknown. In this report, we showed that ADM suppressed reactive oxygen species (ROS) increase by inhibiting reduction of glutathione (GSH) level in hypoxia/r...

journal_title：FEBS letters

authors： Kim SM,Kim JY,Lee S,Park JH

更新日期：2010-01-04 00:00:00

abstract：:Extracellular ATP itself elicited the generation of superoxide (O2-) in guinea pig peritoneal macrophages associated with an increase in cytosolic calcium ([Ca2+]i). The ATP-induced O2- generation was completely inhibited by pretreatment with pertussis toxin (PT) accompanied by the suppression of [Ca2+]i mobilization....

journal_title：FEBS letters

authors： Nakanishi M,Takihara H,Minoru Y,Yagawa K

更新日期：1991-04-22 00:00:00

abstract：:alpha-1-Antitrypsin is found in hepatocytes as a high-mannose glycoprotein (Mr 49 000), extracellularly as a complex-type glycoprotein (Mr 54 000). Deglycosylation of both forms with peptide: N-glycosidase led to proteins of identical app. Mr (41 000). The sequence of 26 N-terminal amino acids of rat alpha 1-antitryps...

journal_title：FEBS letters

authors： Gross V,Kaiser C,Tran-Thi TA,Schmelzer E,Witt I,Plummer TH Jr,Heinrich PC

更新日期：1983-01-24 00:00:00

abstract：:In prokaryotic and eukaryotic organisms, proteins are efficiently sorted to reach their final destinations in a whole range of subcellular compartments. Targeting is mediated by hydrophobic signal sequences or hydrophilic targeting sequences depending upon the compartment, these sequences being often processed. Protei...

journal_title：FEBS letters

authors： Lazdunski CJ,Benedetti H

更新日期：1990-08-01 00:00:00

abstract：:The GTPase RhoA is essential for the development of pre-T cells in the thymus. To investigate the mechanisms used by RhoA to control thymocyte development we have used Affymetrix gene profiling to identify RhoA regulated genes in T cell progenitors. The data show that RhoA plays a specific and essential role in pre-T ...

journal_title：FEBS letters

authors： Mullin M,Lightfoot K,Clarke R,Miller M,Lahesmaa R,Cantrell D

更新日期：2007-09-04 00:00:00

abstract：:The NK-lysin derived peptide NK-2 is a potent antibacterial, but non-toxic to a human keratinocyte cell line and of low hemolytic activity. Its target selectivity is based upon a strong binding preference to membranes containing anionic phospholipids, which are normally not found on the surface of human cells. Here, w...

journal_title：FEBS letters

authors： Schröder-Borm H,Bakalova R,Andrä J

更新日期：2005-11-07 00:00:00

abstract：:Chromogranin A is known to undergo pH induced conformational changes, and the difference in conformation is supposed to be responsible for the difference in Ca2+ binding property. To gain insight regarding the overall structure and the nature of pH-induced conformational changes of chromogranin A, limited trypsin dige...

journal_title：FEBS letters

authors： Yoo SH,Ferretti JA

更新日期：1993-11-22 00:00:00

abstract：:To assess the role of crystallinity in biological response, we quantitated the generation of reactive oxygen metabolites in human polymorphonuclear leukocytes (PMN) with a chemiluminescence assay using three hydroxyapatite preparations with sintering temperatures of 1,200 degrees C and 900 degrees C and a drying tempe...

journal_title：FEBS letters

authors： Nagase M,Nishiya H,Abe Y

更新日期：1993-07-05 00:00:00

abstract：:Clusters of codons pairing to low-abundance tRNAs synchronize the translation with co-translational folding of single domains in multidomain proteins. Although proven with some examples, the impact of the ribosomal speed on the folding and solubility on a global, cell-wide level remains elusive. Here we show that upre...

journal_title：FEBS letters

authors： Fedyunin I,Lehnhardt L,Böhmer N,Kaufmann P,Zhang G,Ignatova Z

更新日期：2012-09-21 00:00:00

abstract：:Sodium azide inhibited multi-site (steady-state) ATPase activity of E. coli F1 more than 90%, but did not affect uni-site (single-site) ATPase activity. Thus azide inhibited multi-site ATPase activity by lowering catalytic cooperativity. Consistent with this observation, azide changed the ligand-induced fluorescence r...

journal_title：FEBS letters

authors： Noumi T,Maeda M,Futai M

更新日期：1987-03-23 00:00:00

abstract：:The accumulation of pre-PhoS under conditions of PhoS overproduction has been previously described. It is now demonstrated that during the induction of PhoS, a delay in the completion of polypeptide chain elongation can be detected. This delay is related to the extent of jamming of export sites by pre-PhoS or by other...

journal_title：FEBS letters

authors： Anba J,Lazdunski C,Pages JM

更新日期：1986-02-03 00:00:00