Nature of the pH-induced conformational changes and exposure of the C-terminal region of chromogranin A.

abstract：:We developed a novel method to quantitatively measure GTP gamma S binding to specific G proteins in crude membranes using G-protein antibodies. The basic strategy was that the materials were initially incubated with [35S]GTP gamma S at 37 degrees C. After 4 degrees C incubation in the wells of an ELISA plate precoated...

journal_title：FEBS letters

authors： Okamoto T,Ikezu T,Murayama Y,Ogata E,Nishimoto I

更新日期：1992-06-29 00:00:00

abstract：:Researchers still have great difficulty in isolating individual ribosomal proteins from the ribosome in quantities high enough for structural research. To this end, when studying protein S7, we created an E. coli overproducer of the recombinant protein S7 of Thermus thermophilus. The vector for expression was pQE-32 h...

journal_title：FEBS letters

authors： Karginov AV,Karginova OA,Spiridonova VA,Kopylov AM

更新日期：1995-08-07 00:00:00

abstract：:The red cell membrane is comprised of a lipid bilayer studded with transmembrane proteins, and laminated by a protein network, the membrane skeleton, at the surface of the inner monolayer. The erythrocyte owes its mechanical properties to the membrane skeleton. Hereditary spherocytosis, hereditary elliptocytosis or po...

journal_title：FEBS letters

authors： Delaunay J

更新日期：1995-08-01 00:00:00

abstract：:Single channel recording techniques have been applied to neurons cultured from the hippocampus and the respiratory area of the brain stem of fetal rats in order to search for nicotinic acetylcholine receptors (nAChR) in the central nervous system. In addition to acetylcholine (ACh), the potent and specific agonist (+)...

journal_title：FEBS letters

authors： Aracava Y,Deshpande SS,Swanson KL,Rapoport H,Wonnacott S,Lunt G,Albuquerque EX

更新日期：1987-09-28 00:00:00

abstract：:Hematopoietic progenitor kinase 1 (HPK1) is a hematopoietic cell-restricted member of the Ste20 serine/threonine kinase super family. We recently reported that HPK1 is involved in c-Jun NH2-terminal kinase (JNK) signaling pathway by sequential activation of MLK3-MKK7-JNK3 after cerebral ischemia. Here, we used 4-amino...

journal_title：FEBS letters

authors： Li T,Yu XJ,Zhang GY

更新日期：2008-06-11 00:00:00

abstract：:Tissue-transglutaminase (t-TGase) is a family of calcium-dependent enzymes. A Ca2+-independent soluble enzyme, in addition to t-TGase, capable of incorporating polyamines into proteins was demonstrated in rat intestinal mucosa. The Ca2+-independent enzyme was stimulated 2- to 5-fold by Fe2+ and Co2+ ions but inhibited...

journal_title：FEBS letters

authors： Tsai YH,Lai WF,Wu YW,Johnson LR

更新日期：1998-09-18 00:00:00

abstract：:To complete the amino acid sequence of GTP:AMP phosphotransferase (MgGTP + AMP in equilibrium with MgGDP + ADP) from beef heart mitochondria it was necessary to sequence an intermediate region of about 33 residues after position 102 [(1984) Eur. J. Biochem. 143, 331-339] and find a suitable overlap with the rest of th...

journal_title：FEBS letters

authors： Tomasselli AG,Frank R,Schiltz E

更新日期：1986-07-07 00:00:00

abstract：:In Clostridium thermoaceticum, the synthesis of acetyl-CoA from methyl tetrahydrofolate occurs via a series of enzymatic reactions involving methyl transferase, corrinoid/Fe-S protein (corrinoid), carbon monoxide dehydrogenase (CODH) and ferredoxin. We have investigated the possibility of one or more of these proteins...

journal_title：FEBS letters

authors： Shanmugasundaram T,Sundaresh CS,Kumar GK

更新日期：1993-07-12 00:00:00

abstract：:The ability of a yeast nuclear protein to be transported into the nucleus of a higher eukaryotic cell was investigated. Mcm1, a transcriptional activator protein from Saccharomyces cerevisiae, was microinjected into the cytoplasm of Xenopus laevis frog oocytes. Mcm1 was imported into the oocyte nucleus indicating that...

journal_title：FEBS letters

authors： Wagner P,Hall MN

更新日期：1993-04-26 00:00:00

abstract：:We investigated insulin's effect on intestinal lipid, transport and, particularly, the biogenesis of apolipoproteins crucial to lipoprotein secretion. Adding insulin (3 mU) to the serum-free medium of cultured jejunal explants from human fetuses (17-20 weeks) reduced triglyceride and chylomicron production and inhibit...

journal_title：FEBS letters

authors： Levy E,Sinnett D,Thibault L,Nguyen TD,Delvin E,Ménard D

更新日期：1996-09-16 00:00:00

abstract：:Glycine, proline and taurine, when present in the range 0.1-0.60 M, inhibit chromatin precipitation by sodium chloride. Histone gel electrophoresis revealed that the linker histones H1 and H5 were largely depleted from the supernatant chromatin fraction at 0.2 M NaCl, while this depletion was absent in the presence of...

journal_title：FEBS letters

authors： Buche A,Ouassaidi A,Hacha R,Delpire E,Gilles R,Houssier C

更新日期：1989-04-24 00:00:00

abstract：:Glycoprotein synthesis is initiated in the endoplasmic reticulum (ER) lumen upon transfer of a glycan (Glc3Man9GlcNAc2) from a lipid derivative to Asn residues (N-glycosylation). N-Glycan-dependent quality control of glycoprotein folding in the ER prevents exit to Golgi of folding intermediates, irreparably misfolded ...

journal_title：FEBS letters

authors： Caramelo JJ,Parodi AJ

更新日期：2015-11-14 00:00:00

abstract：:Mammalian voltage-activated Shaker K+ channels associate with at least three cytoplasmic proteins: Kv beta 1, Kv beta 2 and Kv beta 3. These beta subunits contain variable N-termini, which can modulate the inactivation of Shaker alpha subunits, but are homologous throughout an aldo-keto reductase core. Human and ferre...

journal_title：FEBS letters

authors： McCormack K,McCormack T,Tanouye M,Rudy B,Stühmer W

更新日期：1995-08-14 00:00:00

abstract：:We have previously generated transgenic (Tg) mice expressing the human apolipoprotein (apo) A-I/C-III/A-IV gene cluster. This expression induced hyperlipidemia but reduced atherosclerotic lesions in genetically modified mice lacking apoE. Atherosclerosis is a multifactorial process and environmental factors such as di...

journal_title：FEBS letters

authors： Baroukh N,Ostos MA,Vergnes L,Recalde D,Staels B,Fruchart J,Ochoa A,Castro G,Zakin MM

更新日期：2001-07-27 00:00:00

abstract：:The effects of intracellular purine nucleotides on tolbutamide-induced block of ATP-dependent K+ channels from mouse pancreatic B-cells were studied using the patch-clamp technique. When applied to the inside of excised patches, tolbutamide alone blocked channel activity half-maximally at 55 microM and the concentrati...

journal_title：FEBS letters

authors： Zünkler BJ,Lins S,Ohno-Shosaku T,Trube G,Panten U

更新日期：1988-11-07 00:00:00

abstract：:The ATP-binding cassette (ABC) transporters constitute one of the largest families of proteins in evolution. The ATM1 gene of the yeast Saccharomyces cerevisiae encodes an ABC protein, which is localized to the mitochondrial inner membrane. A deletion of ATM1 results in the accumulation of up to a 30-fold excess of mi...

journal_title：FEBS letters

authors： Chloupková M,Reaves SK,LeBard LM,Koeller DM

更新日期：2004-07-02 00:00:00

abstract：:Study of the relative proportions of ribosomes and polysomes released by a standardized lysing procedure from isogenic RC(str) and RC(rel) strains of Escherichia coli shows that a 20-min period of amino acid starvation of RC(str) bacteria reduces the fraction of ribosomes recovered in polysomes to about 60% of its val...

journal_title：FEBS letters

authors： Matzura H

更新日期：1970-01-15 00:00:00

abstract：:The use of tobacco plants as a novel expression system for the production of human homotrimeric collagen I is presented in this report. Constructs were engineered from cDNA encoding the human proalpha1(I) chain to generate transgenic tobacco plants expressing collagen I. The recombinant proalpha1(I) chains were expres...

journal_title：FEBS letters

authors： Ruggiero F,Exposito JY,Bournat P,Gruber V,Perret S,Comte J,Olagnier B,Garrone R,Theisen M

更新日期：2000-03-03 00:00:00

abstract：:The photoreduction of the quinone (Q) pool in the photosynthetic membrane of the purple bacterium Rhodobacter sphaeroides was investigated by steady-state and time-resolved Fourier transform infrared difference spectroscopy. The results are consistent with the existence of a homogeneous Q pool inside the chromatophore...

journal_title：FEBS letters

authors： Mezzetti A,Leibl W,Breton J,Nabedryk E

更新日期：2003-02-27 00:00:00

abstract：:Benzimidazoles are widely used as anthelmintic agents and systemic fungicides. In susceptible organisms, benzimidazoles bind to beta-tubulin and block microtubule polymerization. To further characterize this interaction, site-directed mutagenesis followed by gene replacement was used to change Saccharomyces cerevisiae...

journal_title：FEBS letters

authors： Li J,Katiyar SK,Edlind TD

更新日期：1996-04-29 00:00:00

abstract：:We have raised monoclonal antibodies (Mab) to the Mr 55,000 desmin polypeptide, electrophoretically purified from cytoskeletal preparations of isolated bovine heart Purkinje fibers. One of the Mabs, 39AB6, revealed desmin only in cow Purkinje fibers and did not react with desmins from other muscle cells, including ven...

journal_title：FEBS letters

authors： Virtanen I,Närvänen O,Thornell LE

更新日期：1990-07-02 00:00:00

abstract：:Specific activity was compared between wild-type (WT) neutral protease from Bacillus stearothermophilus and mutant protease (M1; Gly144 replaced by Ala144) with enhanced thermostability. When casein was used as a substrate, M1 showed 1.5-times higher specific activity than that of WT. In contrast, the specific activit...

journal_title：FEBS letters

authors： Takagi M,Imanaka T

更新日期：1989-08-28 00:00:00

abstract：:Adhesion of platelets to the exposed extracellular matrix proteins at sites of vascular injury is partly regulated by the local fluid shear stress. Because the Leu33Pro (Pl(A)) polymorphism of integrin beta(3) confers only a modest increase in adhesion under static conditions, we used CHO and 293 cells expressing the ...

journal_title：FEBS letters

authors： Vijayan KV,Huang TC,Liu Y,Bernardo A,Dong JF,Goldschmidt-Clermont PJ,Alevriadou BR,Bray PF

更新日期：2003-04-10 00:00:00

abstract：:Protective antigen (PA) is the central receptor binding component of anthrax toxin, which translocates catalytic components of the toxin into the cytosol of mammalian cells. Ever since the crystal structure of PA was solved, there have been speculations regarding the possible role of calcium ions present in domain I o...

journal_title：FEBS letters

authors： Gupta PK,Chandra H,Gaur R,Kurupati RK,Chowdhury S,Tandon V,Singh Y,Maithal K

更新日期：2003-11-20 00:00:00

abstract：:Sodium azide inhibited multi-site (steady-state) ATPase activity of E. coli F1 more than 90%, but did not affect uni-site (single-site) ATPase activity. Thus azide inhibited multi-site ATPase activity by lowering catalytic cooperativity. Consistent with this observation, azide changed the ligand-induced fluorescence r...

journal_title：FEBS letters

authors： Noumi T,Maeda M,Futai M

更新日期：1987-03-23 00:00:00

abstract：:Challenge of Rhodobacter capsulatus cells with the superoxide propagator methyl viologen resulted in the induction of a diaphorase activity identified as a member of the ferredoxin (flavodoxin)-(reduced) nicotinamide adenine dinucleotide phosphate (NADP(H)) reductase (FPR) family by N-terminal sequencing. The gene cod...

journal_title：FEBS letters

authors： Bittel C,Tabares LC,Armesto M,Carrillo N,Cortez N

更新日期：2003-10-23 00:00:00

abstract：:Antibodies to a synthetic peptide from the 'amphipathic helix' of the alpha-chain of the nicotinic acetylcholine receptor (nAChR) bound both to detergent-solubilised and membrane-bound nAChR, indicating that this region, suggested as a component of the transmembrane ion channel in one model, is not buried in the membr...

journal_title：FEBS letters

authors： Roth B,Schwendimann B,Hughes GJ,Tzartos SJ,Barkas T

更新日期：1987-08-31 00:00:00

abstract：:Transfer of naked plasmid with insulin precursor DNA into skeletal muscle of streptozotocin (STZ)-induced diabetic mice through electroporation and detection of gene expression is described. Four different human insulin precursor DNA fragments were inserted into pcDNA3.1(-), downstream of a CMV promoter. Three of them...

journal_title：FEBS letters

authors： Yin D,Tang JG

更新日期：2001-04-20 00:00:00

abstract：:Polyhydroxyalkanoate (PHA) synthases catalyze the polymerization reaction of the acyl moiety of hydroxyacyl-coenzyme A into polyester. The catalytic subunit PhaC of PHA synthase has the PhaC box sequence at the active site that is typically described as G-X-C-X-G-G (X is an arbitrary amino acid), and cysteine is an ac...

journal_title：FEBS letters

authors： Nambu Y,Ishii-Hyakutake M,Harada K,Mizuno S,Tsuge T

更新日期：2020-02-01 00:00:00

abstract：:The amino acid sequence of protein Z has been determined from sequence analysis performed on fragments obtained by chemical and enzymatic degradations. The polypeptide consists of a single chain containing 396 amino acid residues (Mr 43 677). Comparison with the vitamin K-dependent plasma proteins reveals an extensive...

journal_title：FEBS letters

authors： Højrup P,Jensen MS,Petersen TE

更新日期：1985-05-20 00:00:00