Measurement of GTP gamma S binding to specific G proteins in membranes using G-protein antibodies.

abstract：:Islet-activating protein (IAP) suppressed Ca2+-induced histamine release and phospholipase A2 activation in Gpp(NH)p-loaded mast cells. Since the guanine nucleotide-binding protein involved in adenylate cyclase inhibition is known to lose its function upon being ADP-ribosylated by IAP, the nucleotide-binding protein i...

journal_title：FEBS letters

authors： Nakamura T,Ui M

更新日期：1984-08-06 00:00:00

abstract：:Hydrolysis of GTP, bound to members of the G-protein superfamily, terminates their downstream signaling activity. A conserved glutamine serves a critical role in this pivotal guanosine triphosphatase (GTPase) reaction. However, the role of the catalytic glutamine in GTP hydrolysis is still not well understood. We have...

journal_title：FEBS letters

authors： Zor T,Andorn R,Sofer I,Chorev M,Selinger Z

更新日期：1998-08-21 00:00:00

abstract：:Regulators of G protein signaling RGS4 and RGS7 accelerate the kinetics of K(+) channels (GIRKs) in the Xenopus oocyte system. Here, via quantitative analysis of RGS expression, we reveal biphasic effects of RGSs on GIRK regulation. At low concentrations, RGS4 inhibited basal GIRK activity, but stimulated it at high c...

journal_title：FEBS letters

authors： Keren-Raifman T,Bera AK,Zveig D,Peleg S,Witherow DS,Slepak VZ,Dascal N

更新日期：2001-03-09 00:00:00

abstract：:Reconstituted Na+,K+-ATPase from either pig kidney or shark rectal glands was phosphorylated by cAMP dependent protein kinase, PKA. The stoichiometry was approximately 0.9 mol P(i)/mol alpha-subunit in the pig kidney enzyme and approximately 0.2 mol P(i)/mol alpha-subunit in the shark enzyme. In shark, Na+,K+-ATPase P...

journal_title：FEBS letters

authors： Cornelius F,Logvinenko N

更新日期：1996-02-19 00:00:00

abstract：:The interaction of Bordetella pertussis adenylate cyclase toxin (CyaA) with complement receptor 3 (CR3, CD11b/CD18) involves N-linked oligosaccharide chains. To investigate the relative importance of the individual N-glycans of CR3 for toxin activity, the asparagine residues of the consensus N-glycosylation sites of C...

journal_title：FEBS letters

authors： Hasan S,Osickova A,Bumba L,Novák P,Sebo P,Osicka R

更新日期：2015-01-30 00:00:00

abstract：:Several components of translation, e.g. eIF4E and PKR, are implicated in cancer. The e-subunit (p48) of mammalian initiation factor 3 is encoded by the Int6 gene, a common site for integration of the mouse mammary tumor virus genome, leading to the production of a truncated eukaryotic initiation factor-3e (eIF3e). Sta...

journal_title：FEBS letters

authors： Mayeur GL,Hershey JW

更新日期：2002-03-06 00:00:00

abstract：:The complexity of the proteasome gene family in higher plants was investigated by identification and sequencing cDNA clones from the Arabidopsis thaliana database showing homologies to 20S proteasome subunits. We identified plant counterparts for each of the 14 proteasomal subunit subfamilies. Moreover, several of the...

journal_title：FEBS letters

authors： Parmentier Y,Bouchez D,Fleck J,Genschik P

更新日期：1997-10-27 00:00:00

abstract：:2-Deoxyglucose and 5-thioglucose, in the same fashion as glucose, cause the inactivation of the rat hepatocyte glycogen phosphorylase and the activation of glycogen synthase. However, 6-deoxyglucose and 1,5-anhydroglucitol inactivate phosphorylase without increasing the activation state of glycogen synthase. With 3-O-...

journal_title：FEBS letters

authors： Carabaza A,Ciudad CJ,Baqué S,Guinovart JJ

更新日期：1992-01-20 00:00:00

abstract：:The aim of this study was to evaluate the involvement of oxidative stress in glutamate-evoked transglutaminase (TGase) upregulation in astrocyte cultures (14 DIV). A 24 h exposure to glutamate caused a dose-dependent depletion of glutathione intracellular content and increased the ROS production in cell cultures. Thes...

journal_title：FEBS letters

authors： Campisi A,Caccamo D,Li Volti G,Currò M,Parisi G,Avola R,Vanella A,Ientile R

更新日期：2004-12-03 00:00:00

abstract：:The (beta alpha)(8)-barrel is the most frequent and most versatile fold among enzymes [Höcker et al., Curr. Opin. Biotechnol. 12 (2001) 376-381; Wierenga, FEBS Lett. 492 (2001) 193-198]. Structural and functional evidence suggests that (beta alpha)(8)-barrels evolved from an ancestral half-barrel, which consisted of f...

journal_title：FEBS letters

authors： Höcker B,Schmidt S,Sterner R

更新日期：2002-01-16 00:00:00

abstract：:We have isolated a full-length cDNA (HPAsn.6) for human placenta glycosylasparaginase using a 221-bp PCR amplified fragment containing rat liver asparaginase gene sequences. The deduced amino acid sequence from the human clone showed sequence identity to both the alpha and beta subunits of the rat enzyme. The human en...

journal_title：FEBS letters

authors： Fisher KJ,Tollersrud OK,Aronson NN Jr

更新日期：1990-09-03 00:00:00

abstract：:In the methylerythritol phosphate pathway for isoprenoid biosynthesis, the GcpE/IspG enzyme catalyzes the conversion of 2-C-methyl-d-erythritol 2,4-cyclodiphosphate into (E)-4-hydroxy-3-methylbut-2-enyl diphosphate. This reaction requires a double one-electron transfer involving a [4Fe-4S] cluster. A thylakoid prepara...

journal_title：FEBS letters

authors： Seemann M,Tse Sum Bui B,Wolff M,Miginiac-Maslow M,Rohmer M

更新日期：2006-03-06 00:00:00

abstract：:Impaired functioning of the proteasome pathway is one of the molecular mechanism underlying neurodegenerative changes in Alzheimer's disease. In this study, we report that dysfunction of the proteasome pathway in astroglial cells leads to decreased survival and dysregulation of chemokines by differential regulation of...

journal_title：FEBS letters

authors： Choi K,Lee J,Choi C

更新日期：2007-10-02 00:00:00

abstract：:The fraction of polyclonal caldesmon antibodies cross-reacting with rabbit skeletal troponin T are shown to compete with smooth muscle tropomyosin for caldesmon and troponin T, as revealed by ELISA method. The epitope recognized by these antibodies was also found in Mr 77 kDa non-muscle caldesmon. These results provid...

journal_title：FEBS letters

authors： Birukov KG,Shirinsky VP,Vorotnikov AV,Gusev NB

更新日期：1990-03-26 00:00:00

abstract：:Glucocorticoid hormones (GHs) regulate cell proliferation of neural progenitor cells (NPCs) contributing to reduction of neurogenesis after stress. We show here that dexamethasone (Dex) decreases BRUCE/Apollon (BRUCE) in cultured NPCs in a GH-receptor-dependent manner. Downregulation of BRUCE by Dex or using silencing...

journal_title：FEBS letters

authors： Sippel M,Rajala R,Korhonen L,Bornhauser B,Sokka AL,Naito M,Lindholm D

更新日期：2009-07-07 00:00:00

abstract：:In a previous study [(1987) J. Mol. Biol. 194, 663-673], we isolated ten insertion/deletion mutants (indels) of the maltose binding protein for which the maltose binding constant was only a little or not at all affected. In this paper, we have localized these mutations in the recently solved three-dimensional structur...

journal_title：FEBS letters

authors： Betton JM,Martineau P,Saurin W,Hofnung M

更新日期：1993-06-28 00:00:00

abstract：:Casein kinase Ialpha (CKIalpha) belongs to a family of serine/threonine protein kinases involved in membrane trafficking, RNA processing, mitotic spindle formation and cell cycle progression. In this report, we identified several CKIalpha interacting proteins including RCC1, high mobility group proteins 1 and 2 (HMG1,...

journal_title：FEBS letters

authors： Dubois T,Howell S,Zemlickova E,Aitken A

更新日期：2002-04-24 00:00:00

abstract：:Brain-type creatine kinase B-CK (EC 2.7.3.2) was purified from several chicken tissues, e.g. cardiac muscle, brain, gizzard and retina. Two major monomeric chicken B-CK subunits, designated Bb (basic) and Ba (acidic), which differ in isoelectric point, were separated by chromatofocusing in the presence of 8 M urea on ...

journal_title：FEBS letters

authors： Quest AF,Eppenberger HM,Wallimann T

更新日期：1990-03-26 00:00:00

abstract：:Spermatid-specific thioredoxin-1 (Sptrx-1) is the first member of the thioredoxin family of proteins with a tissue-specific expression pattern, found exclusively in the tail of elongating spermatids and spermatozoa. We describe here further biochemical characterization of human Sptrx-1 protein structure and enzymatic ...

journal_title：FEBS letters

authors： Jiménez A,Johansson C,Ljung J,Sagemark J,Berndt KD,Ren B,Tibbelin G,Ladenstein R,Kieselbach T,Holmgren A,Gustafsson JA,Miranda-Vizuete A

更新日期：2002-10-23 00:00:00

abstract：:Numerous bacterial strains produce surface lectins, commonly in the form of fimbriae that are filamentous assemblies of protein subunits. Among the best characterized of these are the type 1 (mannose specific) fimbrial lectins of Escherichia coli that consist almost exclusively of one class of subunit with a molecular...

journal_title：FEBS letters

authors： Sharon N

更新日期：1987-06-15 00:00:00

abstract：:We present an open-access web platform isomiRex, to identify isomiRs and on the fly graphical visualization of the differentially expressed miRNAs in control as well as treated library. The open-access web-platform is not restricted only to NGS sequence dataset from animals and potentially analyzes a wider dataset for...

journal_title：FEBS letters

authors： Sablok G,Milev I,Minkov G,Minkov I,Varotto C,Yahubyan G,Baev V

更新日期：2013-08-19 00:00:00

abstract：:We investigated the expression of formyl peptide receptor (FPR) and its functional role in human bone marrow-derived mesenchymal stem cells (MSCs). We analyzed the expression of FPR by using ligand-binding assay with radio-labeled N-formyl-met-leu-phe (fMLF), and found that MSCs express FPR. FMLF stimulated intracellu...

journal_title：FEBS letters

authors： Kim MK,Min do S,Park YJ,Kim JH,Ryu SH,Bae YS

更新日期：2007-05-01 00:00:00

abstract：:In the mouse macrophage-like cell line RAW 264, vacuolar-type (H(+))-ATPase (V-ATPase) inhibitors, bafilomycin A(1) and concanamycin A, increased the level of cyclooxygenase (COX)-2 protein and its mRNA. The V-ATPase inhibitor-induced expression of COX-2 was suppressed by inhibitors of c-jun N-terminal kinase (JNK) an...

journal_title：FEBS letters

authors： Kamachi F,Yanai M,Ban HS,Ishihara K,Hong J,Ohuchi K,Hirasawa N

更新日期：2007-10-02 00:00:00

abstract：:Abstract Type V adenylyl cyclase (AC) was stably over-expressed in HEK293 cells (293AC-V). Forskolin-stimulated cAMP accumulation in 293AC-V was 5 times as great as that in control cells. PMA, a protein kinase C (PKC) activator, enhanced cAMP accumulation in 293AC-V cells dose-and time-dependently and this enhancement...

journal_title：FEBS letters

authors： Kawabe Ji,Ebina T,Toya Y,Oka N,Schwencke C,Duzic E,Ishikawa Y

更新日期：1996-04-22 00:00:00

abstract：:Structural differences between platelet and endothelial cell thrombospondin (TBSP) were found in two protease-resistant domains (70 and 18 kDa). The 70 kDa fragment is involved in the binding of TBSP to fibrinogen and the 18 kDa fragment in the attachment to various cultured cells. Despite these structural differences...

journal_title：FEBS letters

authors： Clezardin P,Bourdillon MC,Hunter NR,McGregor JL

更新日期：1988-02-15 00:00:00

abstract：:Intracellular vesicular transport involves targeting of vesicles to their correct destination and subsequent fusion with the target membrane. These processes employ unique compartment-specific proteins in combination with general components which act at several transport steps. This review will summarize recent progre...

journal_title：FEBS letters

authors： Söllner T

更新日期：1995-08-01 00:00:00

abstract：:The catalytic cycle of heme peroxidases involves two reactive states, compound I and compound II. Although their reduction potentials at pH 7 are similar, compound I is in general more reactive towards organic substrates than compound II. The different reactivities have until now remained unexplained. In this study, t...

journal_title：FEBS letters

authors： Folkes LK,Candeias LP

更新日期：1997-07-28 00:00:00

abstract：:When bottromycin A2 was added to an in vitro protein synthesis system carried out by naturally occurring polysomes, it inhibited protein synthesis effectively. Examination of the 3 steps of peptide chain elongation revealed that the binding of aminoacyl-tRNA to the polyribosomes was inhibited by bottromycin A2. In con...

journal_title：FEBS letters

authors： Otaka T,Kaji A

更新日期：1983-03-07 00:00:00

abstract：:We have studied the interaction of the yeast RAS2 protein with guanine nucleotides using energetic parameters for the dissociation of RAS.nucleotide complexes. The results indicated that a Gly-->Ser substitution at position 82 led to an altered interaction with GppNHp and, to a lesser extent, also with GDP. It was als...

journal_title：FEBS letters

authors： De Vendittis E,Fasano O

更新日期：1994-06-27 00:00:00

abstract：:Various cDNAs coding for part or all of human pre-pro lactase-phlorizin hydrolase (pre-proLPH) were transfected into COS cells and the subcellular location of the lactase-related proteins assessed. Only the complete proLPH reached the plasma membrane. LPH without the pro sequence, and a construct containing the pro se...

journal_title：FEBS letters

authors： Oberholzer T,Mantei N,Semenza G

更新日期：1993-10-25 00:00:00