Regulation of type V adenylyl cyclase by PMA-sensitive and -insensitive protein kinase C isoenzymes in intact cells.


:Abstract Type V adenylyl cyclase (AC) was stably over-expressed in HEK293 cells (293AC-V). Forskolin-stimulated cAMP accumulation in 293AC-V was 5 times as great as that in control cells. PMA, a protein kinase C (PKC) activator, enhanced cAMP accumulation in 293AC-V cells dose-and time-dependently and this enhancement was abolished by staurosporine. Insulin also enhanced cAMP accumulation in 293AC-V cells. Co-transfection of PKC-zeta, but not PKC-alpha, potentiated the effects of insulin. These data suggest that type V AC activity is regulated in cells by PKC isoenzymes through different extracellular stimuli.




FEBS letters


Kawabe Ji,Ebina T,Toya Y,Oka N,Schwencke C,Duzic E,Ishikawa Y




Has Abstract


1996-04-22 00:00:00














  • Clustering in coated vesicles of polyunsaturated phospholipids segregated from plasma and Golgi membranes of adrenocortical cells.

    abstract::In bovine adrenocortical cells, the fatty acyl chains of the phospholipids have been identified in the membranes of the different cell compartments: plasma membranes, Golgi complex and coated vesicle membranes. An increase in the total number of unsaturation in the fatty acid is demonstrated in the coated vesicle memb...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: de Paillerets C,Bomsel M,Weintraub H,Pépin D,Alfsen A

    更新日期:1987-07-13 00:00:00

  • Conversion of the sodium channel activator aconitine into a potent alpha 7-selective nicotinic ligand.

    abstract::Methyllycaconitine (MLA) is a competitive antagonist of nicotinic acetylcholine receptors, with a remarkable preference for neuronal [125I]alpha Bgt binding sites. We have begun to investigate the structural basis of its potency and subtype selectivity. MLA is a substituted norditerpenoid alkaloid linked to a 2-(methy...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hardick DJ,Cooper G,Scott-Ward T,Blagbrough IS,Potter BV,Wonnacott S

    更新日期:1995-05-22 00:00:00

  • Phosphate regulation of biosynthesis of extracellular RNases of endospore-forming bacteria.

    abstract::The gene for the extracellular ribonuclease of B. pumilus KMM62 (RNase Bp) has been cloned and sequenced. The structural gene for this enzyme is similar to those of the extracellular ribonucleases of B. intermedius 7P (binase) and B. amyloliquefaciens H2 (barnase), as are the regulatory regions of binase and RNase Bp....

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Znamenskaya LV,Gabdrakhmanova LA,Chernokalskaya EB,Leshchinskaya IB,Hartley RW

    更新日期:1995-01-02 00:00:00

  • Overexpression of OsMYB1R1-VP64 fusion protein increases grain yield in rice by delaying flowering time.

    abstract::VP64 is widely used as a transcriptional activator to investigate the biological function of genes, but its potential for application in genetic improvement of crops has not been fully investigated. Here, we characterized an OsMYB1R1-VP64 fusion protein that enhanced the grain yield of rice cultivar 'Kita-ake' by 35%....

    journal_title:FEBS letters

    pub_type: 信件


    authors: Wang J,Wu F,Zhu S,Xu Y,Cheng Z,Wang J,Li C,Sheng P,Zhang H,Cai M,Guo X,Zhang X,Wang C,Wan J

    更新日期:2016-10-01 00:00:00

  • Cell fate determination, neuronal maintenance and disease state: The emerging role of transcription factors Lmx1a and Lmx1b.

    abstract::LIM-homeodomain (LIM-HD) proteins are evolutionary conserved developmental transcription factors. LIM-HD Lmx1a and Lmx1b orchestrate complex temporal and spatial gene expression of the dopaminergic pathway, and evidence shows they are also involved in adult neuronal homeostasis. In this review, the multiple roles play...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Doucet-Beaupré H,Ang SL,Lévesque M

    更新日期:2015-12-21 00:00:00

  • Transbilayer reorientation of platelet-activating factor in the erythrocyte membrane.

    abstract::Platelet-activating factor (PAF) is a phospholipid, intermediate in its structure between diacyl- and lysophospholipids. The reorientation of this highly bioactive compound to the inner membrane layer of human erythrocytes after its primary incorporation into the outer membrane layer has been studied. Reorientation wa...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schneider E,Haest CW,Deuticke B

    更新日期:1986-03-31 00:00:00

  • Continuous hepatocyte growth factor supply prevents lipopolysaccharide-induced liver injury in rats.

    abstract::We present a rat model in which continuous supply of hepatocyte growth factor (HGF) prevents liver injury induced by carbon tetrachloride (CCl4) and E. coli 011:B4 lipopolysaccharide (LPS). Rat fibroblasts genetically modified to secrete rat HGF were implanted in syngenic rat spleen 7 days before administration of the...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kaido T,Yamaoka S,Seto S,Funaki N,Kasamatsu T,Tanaka J,Nakamura T,Imamura M

    更新日期:1997-07-14 00:00:00

  • Arginine kinase overexpression improves Trypanosoma cruzi survival capability.

    abstract::Arginine kinase catalyzes the reversible transphosphorylation between adenosine diphosphate (ADP) and phosphoarginine, which is involved in temporal and spatial adenosine triphosphate (ATP) buffering. Here we demonstrate that the homologous overexpression of the Trypanosoma cruzi arginine kinase improves the ability o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pereira CA,Alonso GD,Ivaldi S,Silber AM,Alves MJ,Torres HN,Flawiá MM

    更新日期:2003-11-06 00:00:00

  • Peroxisomal-microsomal communication in unsaturated fatty acid metabolism.

    abstract::The addition of 1-acyl-sn-glycero-3-phosphocholine (1-acyl-GPC) to peroxisomes decreased the production of acid-soluble radioactivity formed by beta-oxidation of [1-(14)C]arachidonate due to substrate removal by esterification into the acceptor. This peroxisomal-associated acyl-CoA:1-acyl-GPC acyltransferase activity ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Baykousheva SP,Luthria DL,Sprecher H

    更新日期:1995-06-26 00:00:00

  • Elevated citrate levels in non-alcoholic fatty liver disease: the potential of citrate to promote radical production.

    abstract::Plasma citrate levels were found to be elevated in non-alcoholic fatty liver disease (NAFLD) patients. Cellular experiments indicated that increased citrate levels might originate from an excess of fatty acids. The impact of elevated citrate levels on oxidative stress was examined. It was found that citrate stimulated...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: van de Wier B,Balk JM,Haenen GR,Giamouridis D,Bakker JA,Bast BC,den Hartog GJ,Koek GH,Bast A

    更新日期:2013-08-02 00:00:00

  • Volume-dependent and NEM-stimulated K+,Cl- transport is elevated in oxygenated SS, SC and CC human red cells.

    abstract::Mechanisms involved in cell volume regulation are important in SS, SC cells as they might be involved in determining the extent of sickling and the generation of dense cells and irreversibly sickled cells. We have studied in these cells the response to cell swelling of the K+,Cl- transporter. We found that Hb SS, SC a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Canessa M,Spalvins A,Nagel RL

    更新日期:1986-05-05 00:00:00

  • Specific effects of ATP on the kinetics of reconstituted bovine heart cytochrome-c oxidase.

    abstract::Bovine heart cytochrome-c oxidase was reconstituted in liposomes and the kinetics of cytochrome c oxidation were measured by the polarographic and photometric method under uncoupled conditions in the presence of various polyvalent anions. In order to distinguish between specific and unspecific ionic effects of ATP, th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hüther FJ,Kadenbach B

    更新日期:1986-10-20 00:00:00

  • Interaction between calmodulin and microtubule-associated proteins prepared at different stages of brain development.

    abstract::Rat brain microtubules were prepared at the adult stage and from immature (i.e., 4-day-old) animals. At an early stage of development, the composition of microtubule-associated proteins is qualitatively different from that found at the adult stage [(1982) Eur. J. Biochem. 129, 465-471]. The influence of calmodulin on ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Erneux C,Passareiro H,Nunez J

    更新日期:1984-07-09 00:00:00

  • Proteinases of betaretroviruses bind single-stranded nucleic acids through a novel interaction module, the G-patch.

    abstract::Retroviral proteinases (PRs) are essential for retrovirus infectivity but the mechanism of their activity regulation is poorly understood. We investigated possible involvement in this process of the C-terminal domain (CTD) of betaretroviral PRs. We found that the presence of CTD attenuates proteolytic activity of Maso...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Svec M,Bauerová H,Pichová I,Konvalinka J,Strísovský K

    更新日期:2004-10-08 00:00:00

  • Linguistic analysis of protein folding.

    abstract::Folding of nascent chains resembles the decoding of spoken language in that information is emitted as a unidirectional, one-dimensional string of elements, with higher structures and long-distance interactions emerging with time. Applying a "pseudolinguistic' analysis of structure to a set of all 36 possible six-stran...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gross M

    更新日期:1996-07-29 00:00:00

  • Akt mediates TIGAR induction in HeLa cells following PFKFB3 inhibition.

    abstract::Neoplastic cells metabolize higher amounts of glucose relative to normal cells in order to cover increased energetic and anabolic needs. Inhibition of the glycolytic enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) diminishes cancer cell proliferation and tumour growth in animals. In this work, w...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Simon-Molas H,Calvo-Vidal MN,Castaño E,Rodríguez-García A,Navarro-Sabaté À,Bartrons R,Manzano A

    更新日期:2016-09-01 00:00:00

  • ING4 induces G2/M cell cycle arrest and enhances the chemosensitivity to DNA-damage agents in HepG2 cells.

    abstract::The known members of inhibitor of growth (ING) gene family are considered as candidate tumor suppressor genes. ING4, a novel member of ING family, is recently reported to interact with tumor suppressor p53, p300 (a major component of histone acetyl transferase complexes), and p65(RelA) subunit of NF-kappaB. In this st...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zhang X,Xu LS,Wang ZQ,Wang KS,Li N,Cheng ZH,Huang SZ,Wei DZ,Han ZG

    更新日期:2004-07-16 00:00:00

  • Rat prolactin synthesis by lactating mammary epithelial cells.

    abstract::It has previously been suggested that the mammary cell could produce prolactin (PRL). This hypothesis was investigated by incubation with [35S]methionine-cysteine followed by SDS-PAGE, immunoblotting and autoradiography of immunoprecipitated PRL, and by electron microscopic analysis after incubation without or with cy...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lkhider M,Delpal S,Le Provost F,Ollivier-Bousquet M

    更新日期:1997-01-20 00:00:00

  • A role for a pertussis toxin-sensitive trimeric G-protein in store-operated Ca2+ inflow in hepatocytes.

    abstract::The mechanism of store-operated Ca2+ inflow in hepatocytes was investigated using fluo-3 and fura-2 to monitor changes in the concentration of intracellular free Ca2+ in single cells, and 1-(alpha-glycerophosphoryl)-myo-inositol 4,5-diphosphate, P4(5)-1-(2-nitrophenyl)ethyl ester ('caged' GPIP2) and 'caged' guanosine ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Berven LA,Barritt GJ

    更新日期:1994-06-13 00:00:00

  • Fluorescence-detected interactions of oligonucleotides in RecA complexes.

    abstract::A technique has been developed to probe directly RecA-DNA interactions by the use of the fluorescent chromophore, (+)anti-benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE), covalently attached to DNA. The 24-mer oligonucleotide 5'-d(CTACTAAACATGTACAAATCATCC) was specifically modified on the exocyclic nitrogen of the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wittung P,Funk M,Jernström B,Nordén B,Takahashi M

    更新日期:1995-07-10 00:00:00

  • In vitro dissociation of self-assembly of three chaperonin 60s: the role of ATP.

    abstract::A comparative study has investigated the in vitro dissociation and self-assembly of chaperonin 60 14-mers isolated from E. coli (GroEL), yeast mitochondria and pea chloroplasts. In all cases Mg2+ inhibits, and low temperature stimulates, the urea-induced dissociation. ATP or ADP in the presence of Mg2+ enhance the dis...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lissin NM

    更新日期:1995-03-13 00:00:00

  • Evidence for two H2O2-binding sites in ferric cytochrome c oxidase. Indication to the O-cycle?

    abstract::H2O2 addition to the oxidized cytochrome c oxidase reconstituted in liposomes brings about a red shift of the Soret band of the enzyme and an increased absorption in the visible region with two distinct peaks at approximately 570 and 605 nm. Throughout pH range 6-8.5, the spectral changes at 570 nm and in the Soret ba...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vygodina T,Konstantinov AA

    更新日期:1987-07-27 00:00:00

  • Dimeric structure of H(+)-translocating pyrophosphatase from pumpkin vacuolar membranes.

    abstract::Vacuolar membrane H(+)-translocating pyrophosphatase (H(+)-PPase) was purified from pumpkin seedlings. Its enzymatic properties including molecular size of constituting polypeptide (75 kDa) were very similar to those of mung bean H(+)-PPase [(1989) J. Biol. Chem. 264, 20068-20073]. The native, functional molecular siz...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sato MH,Maeshima M,Ohsumi Y,Yoshida M

    更新日期:1991-09-23 00:00:00

  • Pyrroloquinoline-quinone: a reactive oxygen species scavenger in bacteria.

    abstract::Transgenic Escherichia coli expressing pyrroloquinoline-quinone (PQQ) synthase gene from Deinococcus radiodurans showed superior survival during Rose Bengal induced oxidative stress. Such cells showed significantly low levels of protein carbonylation as compared to non-transgenic control. In vitro, PQQ reacted with re...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Misra HS,Khairnar NP,Barik A,Indira Priyadarsini K,Mohan H,Apte SK

    更新日期:2004-12-03 00:00:00

  • Retinal dehydrogenase gene expression in stomach and small intestine of rats during postnatal development and in vitamin A deficiency.

    abstract::Retinal dehydrogenase (RALDH) catalyzes the oxidation of retinal to all-trans and 9-cis retinoic acid, which function as ligands controlling RAR and RXR nuclear receptor-signaling pathways. We have recently shown the expression of RALDH transcript in the stomach and small intestine by reverse transcription polymerase ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bhat PV

    更新日期:1998-04-17 00:00:00

  • Expression of a Cu,Zn superoxide dismutase typical of familial amyotrophic lateral sclerosis induces mitochondrial alteration and increase of cytosolic Ca2+ concentration in transfected neuroblastoma SH-SY5Y cells.

    abstract::We have set up a model system for familial amyotrophic lateral sclerosis (FALS) by transfecting human neuroblastoma cell line SH-SY5Y with plasmids directing constitutive expression of either wild-type human Cu,Zn superoxide dismutase (Cu,ZnSOD) or a mutant of this enzyme (G93A) associated with FALS. We have tested mi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Carrì MT,Ferri A,Battistoni A,Famhy L,Gabbianelli R,Poccia F,Rotilio G

    更新日期:1997-09-08 00:00:00

  • Redoxal as a new lead structure for dihydroorotate dehydrogenase inhibitors: a kinetic study of the inhibition mechanism.

    abstract::Mitochondrial dihydroorotate dehydrogenase (DHOdehase; EC is a target of anti-proliferative, immunosuppressive and anti-parasitic agents. Here, redoxal, (2,2'-[3,3'-dimethoxy[1, 1'-biphenyl]-4,4'-diyl)diimino]bis-benzoic acid, was studied with isolated mitochondria and the purified recombinant human and rat...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Knecht W,Löffler M

    更新日期:2000-02-04 00:00:00

  • The inhibitory effect of nitrite, a stable product of nitric oxide (NO) formation, on arginase.

    abstract::Macrophages contain arginase and an inducible NO synthase, demonstrated by using L-arginine, the common substrate, for production of both nitric oxide and urea. Arginase was inhibited by nitrite, the stable end product of NO. This inhibition was non-competitive, and could not be explained by the reaction of nitrite wi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hrabák A,Bajor T,Temesi A,Mészáros G

    更新日期:1996-07-22 00:00:00

  • A novel subunit of axonemal dynein conserved among lower and higher eukaryotes.

    abstract::To elucidate the subunit composition of axonemal inner-arm dynein, we examined a 38 kDa protein (p38) co-purified with a Chlamydomonas inner arm subspecies, dynein d. We found it is a novel protein conserved among a variety of organisms with motile cilia and flagella. Immunoprecipitation using specific antibody verifi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yamamoto R,Yanagisawa HA,Yagi T,Kamiya R

    更新日期:2006-11-27 00:00:00

  • Cyclooxygenase-2 induction by adiponectin is regulated by a sphingosine kinase-1 dependent mechanism in cardiac myocytes.

    abstract::The adipose-derived plasma protein, adiponectin (APN), has various protective effects on cardiovascular diseases. In this study, we show that endogenous APN is required for full cyclooxygenase-2 (COX-2) induction by ischemia-reperfusion injury in the heart in vivo. In rat neonatal cardiac myocytes, APN-induced COX-2 e...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ikeda Y,Ohashi K,Shibata R,Pimentel DR,Kihara S,Ouchi N,Walsh K

    更新日期:2008-04-02 00:00:00