Abstract:
:Abstract Type V adenylyl cyclase (AC) was stably over-expressed in HEK293 cells (293AC-V). Forskolin-stimulated cAMP accumulation in 293AC-V was 5 times as great as that in control cells. PMA, a protein kinase C (PKC) activator, enhanced cAMP accumulation in 293AC-V cells dose-and time-dependently and this enhancement was abolished by staurosporine. Insulin also enhanced cAMP accumulation in 293AC-V cells. Co-transfection of PKC-zeta, but not PKC-alpha, potentiated the effects of insulin. These data suggest that type V AC activity is regulated in cells by PKC isoenzymes through different extracellular stimuli.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kawabe Ji,Ebina T,Toya Y,Oka N,Schwencke C,Duzic E,Ishikawa Ydoi
10.1016/0014-5793(96)00331-6subject
Has Abstractpub_date
1996-04-22 00:00:00pages
273-6issue
3eissn
0014-5793issn
1873-3468pii
0014579396003316journal_volume
384pub_type
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