Regulation of type V adenylyl cyclase by PMA-sensitive and -insensitive protein kinase C isoenzymes in intact cells.


:Abstract Type V adenylyl cyclase (AC) was stably over-expressed in HEK293 cells (293AC-V). Forskolin-stimulated cAMP accumulation in 293AC-V was 5 times as great as that in control cells. PMA, a protein kinase C (PKC) activator, enhanced cAMP accumulation in 293AC-V cells dose-and time-dependently and this enhancement was abolished by staurosporine. Insulin also enhanced cAMP accumulation in 293AC-V cells. Co-transfection of PKC-zeta, but not PKC-alpha, potentiated the effects of insulin. These data suggest that type V AC activity is regulated in cells by PKC isoenzymes through different extracellular stimuli.




FEBS letters


Kawabe Ji,Ebina T,Toya Y,Oka N,Schwencke C,Duzic E,Ishikawa Y




Has Abstract


1996-04-22 00:00:00














  • Cloning and sequence of full-length cDNAs encoding the human neuronal nicotinic acetylcholine receptor (nAChR) subunits beta3 and beta4 and expression of seven nAChR subunits in the human neuroblastoma cell line SH-SY5Y and/or IMR-32.

    abstract::Using PCR-based techniques, we have isolated and sequenced the full-length cDNAs that encode the human neuronal nAChR subunits alpha3,4,5,7 and beta2,3,4 in the neuroblastoma cell lines SH-SY5Y and/or IMR-32. The predicted nAChR beta3- and beta4-subunit proteins contain 458 and 498 amino acids, respectively. Except fo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Groot Kormelink PJ,Luyten WH

    更新日期:1997-01-06 00:00:00

  • Methionine acts as a "magnet" in photoaffinity crosslinking experiments.

    abstract::Photoaffinity crosslinking has been utilized to probe the nature of the ligand-receptor interface for a number of G protein-coupled receptor systems. Often the photoreactive benzophenone moiety incorporated in the ligand is found to react with a methionine in the receptor. We introduced methionines one-at-a-time into ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wittelsberger A,Thomas BE,Mierke DF,Rosenblatt M

    更新日期:2006-03-20 00:00:00

  • The human lactase-phlorizin hydrolase gene is located on chromosome 2.

    abstract::The lactase-phlorizin hydrolase gene was assigned to chromosome 2 by analysis of Southern blots of DNA from a panel of human-rodent cell hybrids containing characteristic sets of human chromosomes The hybridization probe used was a recently isolated cDNA clone of the human lactase-phlorizin hydrolase gene. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kruse TA,Bolund L,Grzeschik KH,Ropers HH,Sjöström H,Norén O,Mantei N,Semenza G

    更新日期:1988-11-21 00:00:00

  • High and low affinity receptors mediate growth effects of gastrin and gastrin-Gly on DLD-1 human colonic carcinoma cells.

    abstract::Gastrin (G17) and N-carboxymethylgastrin (G17-Gly) have been shown to stimulate the growth of colon cancer cells both in vivo and in vitro. The identity of the receptor mediating these effects is controversial. A recent study demonstrated the presence of a low affinity binding site for G17 and G17-Gly on the DLD-1 hum...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ahmed S,Budai B,Herédi-Szabó K,Farkas J,Tóth G,Murphy RF,Lovas S

    更新日期:2004-01-02 00:00:00

  • The human homologue of the yeast mitochondrial AAA metalloprotease Yme1p complements a yeast yme1 disruptant.

    abstract::In yeast, three AAA superfamily metalloproteases (Yme1p, Afg3p and Rca1p) are localized to the mitochondrial inner membrane where they perform roles in the assembly and turnover of the respiratory chain complexes. We have investigated the function of the proposed human orthologue of yeast Yme1p, encoded by the YME1L g...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Shah ZH,Hakkaart GA,Arku B,de Jong L,van der Spek H,Grivell LA,Jacobs HT

    更新日期:2000-08-04 00:00:00

  • Molecular cloning of the full-length cDNA encoding the human calbindin-D9k.

    abstract::The full-length cDNA encoding the human calbindin-D9k (CaBP-9k) has been cloned using reverse transcription/PCR methodology with rat- and bovine-derived primers and intestinal RNA. A core product, and both a 5' and 3' product encompassing the full-length cDNA were obtained. The clones include coding region for 79 amin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jeung EB,Krisinger J,Dann JL,Leung PC

    更新日期:1992-07-28 00:00:00

  • Rapid reduction in parvalbumin concentration during chronic stimulation of rabbit fast twitch muscle.

    abstract::Chronic indirect stimulation of fast twitch rabbit muscle induced a rapid reduction in parvalbumin concentration. When compared to the unstimulated contralateral muscle, parvalbumins were reduced to 55% following 6 days of stimulation. Prolonged stimulation further reduced parvalbumins so that they were undetectable a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Klug G,Reichmann H,Pette D

    更新日期:1983-02-21 00:00:00

  • The SUPERMAN protein is an active repressor whose carboxy-terminal repression domain is required for the development of normal flowers.

    abstract::SUPERMAN was identified as a putative regulator of transcription that acts in floral development, but its function remains to be clarified. We demonstrate here that SUPERMAN is an active repressor whose repression domain is located in the carboxy-terminal region. Ectopic expression of SUPERMAN that lacked the repressi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hiratsu K,Ohta M,Matsui K,Ohme-Takagi M

    更新日期:2002-03-13 00:00:00

  • Mutational analysis of 6-aminohexanoate-dimer hydrolase: relationship between nylon oligomer hydrolytic and esterolytic activities.

    abstract::Carboxylesterase (EII') from Arthrobacter sp. KI72 has 88% homology to 6-aminohexanoate-dimer hydrolase (EII) and possesses ca. 0.5% of the level of 6-aminohexanoate-linear dimer (Ald)-hydrolytic activity of EII. To study relationship between Ald-hydrolytic and esterolytic activities, random mutations were introduced ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ohki T,Wakitani Y,Takeo M,Yasuhira K,Shibata N,Higuchi Y,Negoro S

    更新日期:2006-09-18 00:00:00

  • Site-specific mutagenesis of the reaction centre from Rhodobacter sphaeroides studied by Fourier transform Raman spectroscopy: mutations at tyrosine M210 do not affect the electronic structure of the primary donor.

    abstract::The effects of mutation of residue tyrosine M210 on the primary donor bacteriochlorophylls have been investigated by near infrared FT-Raman spectroscopy in reaction centres purified from an antenna-deficient strain of Rhodobacter sphaeroides. We find that mutation at the M210 position does not significantly perturb th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jones MR,Heer-Dawson M,Mattioli TA,Hunter CN,Robert B

    更新日期:1994-02-14 00:00:00

  • Characterization of cytosolic malic enzyme in human tumor cells.

    abstract::Cytosolic NADP(+)-dependent malic enzyme (ME) from human tumor cells was characterized in detail and compared to ME from normal human tissues produced in recombinant E. coli. Kinetic properties, size as seen in SDS gels, and HPLC elution profiles of tryptic digests of human 'normal cell' ME and NADP(+)-ME from tumor c...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Loeber G,Dworkin MB,Infante A,Ahorn H

    更新日期:1994-05-16 00:00:00

  • The Saccharomyces cerevisiae DNA damage checkpoint is required for efficient repair of double strand breaks by non-homologous end joining.

    abstract::In this work we report that the Saccharomyces cerevisiae RAD9, RAD24, RAD17, MEC1, MEC3 and RAD53 checkpoint genes are required for efficient non-homologous end joining (NHEJ). RAD9 and RAD24 function additionally in this process. Defective NHEJ in rad9Delta-rad24Delta, but not yku80Delta cells, is only partially resc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: de la Torre-Ruiz M,Lowndes NF

    更新日期:2000-02-11 00:00:00

  • Creutzfeldt-Jakob infection increases adenylate cyclase activity in specific regions of guinea pig brain.

    abstract::Creutzfeldt-Jakob disease is a slow, infectious, progressive neurological disorder which results in human dementia. Synaptic membranes from various brain regions of guinea pigs infected with Creutzfeldt-Jakob disease show increased guanyl nucleotide- or 5-hydroxytryptamine-mediated activation of adenylate cyclase. Thi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rasenick MM,Valley S,Manuelidis EE,Manuelidis L

    更新日期:1986-03-17 00:00:00

  • CD4 receptor binding peptides that block HIV infectivity cause human monocyte chemotaxis. Relationship to vasoactive intestinal polypeptide.

    abstract::The octapeptide Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr (peptide T) and two structural analogs are potent agonists of human monocyte chemotaxis, evincing identical rank potency orders as was previously shown for their inhibition of human immunodeficiency virus (HIV) envelope binding and T cell infectivity. Chemotactic activit...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ruff MR,Martin BM,Ginns EI,Farrar WL,Pert CB

    更新日期:1987-01-19 00:00:00

  • Cyclic AMP raises cytosolic Ca2+ and promotes Ca2+ influx in a clonal pancreatic beta-cell line (HIT T-15).

    abstract::The effect on cytosolic Ca2+ concentration ([Ca2+]i) of cAMP analogues and the adenylate cyclase-stimulating agents forskolin, isoproterenol and glucagon has been examined in an insulin-secreting beta-cell line (HIT T-15) using fura 2. All these manipulations of the cAMP messenger system promoted a rise in [Ca2+]i whi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Prentki M,Glennon MC,Geschwind JF,Matschinsky FM,Corkey BE

    更新日期:1987-08-10 00:00:00

  • Effects of growth hormone-releasing factor, somatostatin and dopamine on growth hormone and prolactin secretion from cultured ovine pituitary cells.

    abstract::A synthetic form of human pancreatic growth hormone releasing factor (GRF-44-NH2) was shown to be a potent stimulator of growth hormone (GH) secretion and cellular cyclic AMP levels in cultured sheep pituitary cells. A small dose-dependent stimulation of prolactin secretion was also observed. Somatostatin (0.5 microM)...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Law GJ,Ray KP,Wallis M

    更新日期:1984-01-23 00:00:00

  • m-Calpain subunits remain associated in the presence of calcium.

    abstract::The hypothesis that calpain subunits dissociate in the presence of Ca2+ has been tested by methods which avoid interference by Ca2+-induced aggregation and large subunit autolysis. Inactive Cys105Ser-m-calpain, bound either to Ni-NTA-agarose or to immobilized casein, after incubation with Ca2+, could be recovered in h...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dutt P,Arthur JS,Croall DE,Elce JS

    更新日期:1998-10-09 00:00:00

  • Lipopolysaccharide-binding protein mediates CD14-independent intercalation of lipopolysaccharide into phospholipid membranes.

    abstract::Lipopolysaccharides (LPS, endotoxin) stimulate mononuclear cells to release cytokines which initiate endotoxic effects. Interaction of LPS at low concentrations with target cells is CD14-dependent whereas at high LPS concentrations it is CD14-independent. Here, we demonstrate by resonance energy transfer (RET) techniq...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schromm AB,Brandenburg K,Rietschel ET,Flad HD,Carroll SF,Seydel U

    更新日期:1996-12-16 00:00:00

  • Ganglioside antigen of DU-PAN-2 in a human pancreatic cancer.

    abstract::DU-PAN-2 reactive gangliosides were isolated from the tumor of a patient with pancreatic cancer (duct cell carcinoma, moderately differentiated adenocarcinoma), having a negative Lewis blood phenotype, and were analyzed by means of TLC-immunostaining, enzyme-linked immunosorbent assay (ELISA), permethylation study, 1H...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hamanaka Y,Hamanaka S,Shinagawa Y,Suzuki T,Inagaki F,Suzuki M,Suzuki A

    更新日期:1994-10-10 00:00:00

  • Sequence and detailed organization of the human caveolin-1 and -2 genes located near the D7S522 locus (7q31.1). Methylation of a CpG island in the 5' promoter region of the caveolin-1 gene in human breast cancer cell lines.

    abstract::The CA microsatellite repeat marker, D7S522, is located at the center of a approximately 1000 kb smallest common deleted region that is lost in many forms of human cancer. It has been proposed that a putative tumor suppressor gene lies in close proximity to D7S522, within this smallest common deleted region. However, ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Engelman JA,Zhang XL,Lisanti MP

    更新日期:1999-04-09 00:00:00

  • Primary and post-irradiation inactivation of the sulfhydryl enzyme malate synthase: correlation of protective effects of additives.

    abstract::The presence of additives during X-irradiation of malate synthase led to radioprotective effects against primary and post-irradiation inactivation. Pronounced effects were provided by typical scavengers, sulfhydryl reagents and specific ligands (substrates, products, analogues). The results show that scavenging and sp...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Durchschlag H,Zipper P

    更新日期:1988-09-12 00:00:00

  • ING4 induces G2/M cell cycle arrest and enhances the chemosensitivity to DNA-damage agents in HepG2 cells.

    abstract::The known members of inhibitor of growth (ING) gene family are considered as candidate tumor suppressor genes. ING4, a novel member of ING family, is recently reported to interact with tumor suppressor p53, p300 (a major component of histone acetyl transferase complexes), and p65(RelA) subunit of NF-kappaB. In this st...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zhang X,Xu LS,Wang ZQ,Wang KS,Li N,Cheng ZH,Huang SZ,Wei DZ,Han ZG

    更新日期:2004-07-16 00:00:00

  • Modulation of phosphoinositide 3-kinase activation by cholesterol level suggests a novel positive role for lipid rafts in lysophosphatidic acid signalling.

    abstract::Methyl-beta-cyclodextrin (MbetaCD) was used to explore a role for cholesterol-enriched plasma membrane microdomains in coupling lysophosphatidic acid (LPA) stimulation to phosphoinositide 3-kinase (PI3K) activation. Cholesterol depletion strongly inhibited the production of phosphatidylinositol 3,4-bisphosphate and ph...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Peres C,Yart A,Perret B,Salles JP,Raynal P

    更新日期:2003-01-16 00:00:00

  • Interleukin-4 stimulates collagen gene expression in human fibroblast monolayer cultures. Potential role in fibrosis.

    abstract::A role for the cytokines produced by tissue-infiltrated inflammatory cells (mainly T-lymphocytes and mast cells) in the pathophysiology of fibrosis has been suggested by several groups. Among the products of these cells, interleukin-4 (IL-4) might be one of the factors involved in the initiation of the fibrotic proces...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gillery P,Fertin C,Nicolas JF,Chastang F,Kalis B,Banchereau J,Maquart FX

    更新日期:1992-05-18 00:00:00

  • Specific binding sites for 3,3'-diiodo-L-thyronine (3,3'-T2) in rat liver mitochondria.

    abstract::Specific binding sites for 3,3'-T2 can be detected in swollen and osmotically treated mitochondria (OTM) from normal and hypothyroid rat liver. In hypothyroid animals, maximal values of binding were obtained at 0 degrees C while values were lower at 37 degrees C and no specific binding could be observed at 60 degrees ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lanni A,Moreno M,Horst C,Lombardi A,Goglia F

    更新日期:1994-09-05 00:00:00

  • Bacterial lectins, cell-cell recognition and infectious disease.

    abstract::Numerous bacterial strains produce surface lectins, commonly in the form of fimbriae that are filamentous assemblies of protein subunits. Among the best characterized of these are the type 1 (mannose specific) fimbrial lectins of Escherichia coli that consist almost exclusively of one class of subunit with a molecular...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Sharon N

    更新日期:1987-06-15 00:00:00

  • Spectral imaging and linear un-mixing enables improved FRET efficiency with a novel GFP2-YFP FRET pair.

    abstract::Spectral variants of the green fluorescent protein (GFP) have been extensively used as reporters to image molecular interactions in living cells by fluorescence resonance energy transfer (FRET). However, those GFP variants which are the most efficient donor acceptor pairs for FRET measurements show a high degree of sp...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zimmermann T,Rietdorf J,Girod A,Georget V,Pepperkok R

    更新日期:2002-11-06 00:00:00

  • Gene relaxation and aging: changes in the abundance of rat ventral prostate SGP-2 (clusterin) and ornithine decarboxylase mRNAs.

    abstract::Sulfated glycoprotein 2 (SGP-2) mRNA progressively increased in the ventral prostate of the aging rat, reaching, at 24 months, 4-fold higher than at 3 months. Ornithine decarboxylase (ODC) mRNA peaked at 6 months (4-fold increase), and at 12 and 24 months was maintained at higher levels than at 3 months. ODC enzymatic...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bettuzzi S,Strocchi P,Marinelli M,Astancolle S,Davalli P,Corti A

    更新日期:1994-07-18 00:00:00

  • SABP2, a methyl salicylate esterase is required for the systemic acquired resistance induced by acibenzolar-S-methyl in plants.

    abstract::Tobacco SABP2, a 29kDa protein catalyzes the conversion of methyl salicylic acid (MeSA) into salicylic acid (SA) to induce SAR. Pretreatment of plants with acibenzolar-S-methyl (ASM), a functional analog of salicylic acid induces systemic acquired resistance (SAR). Data presented in this paper suggest that SABP2 catal...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tripathi D,Jiang YL,Kumar D

    更新日期:2010-08-04 00:00:00

  • TIMP-1 protein expression is stimulated by IL-1 beta and IL-6 in primary rat hepatocytes.

    abstract::Degradation of extracellular matrix proteins is performed by metalloproteinases which are inhibited by tissue inhibitors of metalloproteinases (TIMP). We expressed the murine TIMP-1 protein in E. coli and prepared a polyclonal antiserum against the recombinant protein. Using this antiserum we studied the biosynthesis ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Roeb E,Graeve L,Müllberg J,Matern S,Rose-John S

    更新日期:1994-07-25 00:00:00