Regulation of type V adenylyl cyclase by PMA-sensitive and -insensitive protein kinase C isoenzymes in intact cells.

abstract：:The effects of mildly acidic conditions on the free energy of unfolding (DeltaG(u)(buff)) of the pore-forming alpha-hemolysin (alphaHL) from Staphylococcus aureus were assessed between pH 5.0 and 7.5 by measuring intrinsic tryptophan fluorescence, circular dichroism and elution time in size exclusion chromatography du...

journal_title：FEBS letters

authors： Bortoleto RK,Ward RJ

更新日期：1999-10-15 00:00:00

abstract：:Several studies have indicated the existence of direct effects of melatonin on platelets. Here we show that, melatonin at high concentration is capable of significantly raising platelet intracellular calcium even in the absence of an agonist. The effect of melatonin on platelets was abolished by luzindole, a melatonin...

journal_title：FEBS letters

authors： Kumari S,Dash D

更新日期：2011-07-21 00:00:00

abstract：:Upon dimerization by oxidation, Hsp33 functions as a molecular chaperone in prokaryotes. Previously published structures of both the inactive and active species are of doubtful relevance to the solution conformations since the inactive (reduced) crystal structure was dimeric, while the active (oxidized) species was cr...

journal_title：FEBS letters

authors： Lee YS,Ryu KS,Kim SJ,Ko HS,Sim DW,Jeon YH,Kim EH,Choi WS,Won HS

更新日期：2012-02-17 00:00:00

abstract：:The GAL11 gene encodes an auxiliary transcription factor required for full expression of many, if not all, genes of the yeast Saccharomyces cerevisiae. We have recently shown that GAL11-encoded protein (Gal11p) enhances basal transcription from the CYC1 promoter in a cell-free transcription system [(1993) Proc. Natl. ...

journal_title：FEBS letters

authors： Sakurai H,Ohishi T,Amakasu H,Fukasawa T

更新日期：1994-09-05 00:00:00

abstract：:WT1 encodes a zinc finger protein with a key role in urogenital development that is inactivated in a subset of Wilms' tumors. This tumor suppressor gene product contains an amino-terminal dimerization domain required for trans-inhibition of wild-type WT1 activity by mutants defective for DNA binding. In the course of ...

journal_title：FEBS letters

authors： Bruening W,Moffett P,Chia S,Heinrich G,Pelletier J

更新日期：1996-09-09 00:00:00

abstract：:Nuclear magnetic resonance (NMR) spectroscopy was used to determine the secondary structure and the three-dimensional polypeptide backbone fold of the human sterol carrier protein 2 (hSCP2), which is a basic protein with 123 residues believed to participate in the intracellular transport of cholesterol and various oth...

journal_title：FEBS letters

authors： Szyperski T,Scheek S,Johansson J,Assmann G,Seedorf U,Wüthrich K

更新日期：1993-11-29 00:00:00

abstract：:Transcarboxylase from Propionibacterium shermanii is a complex biotin-containing enzyme composed of 30 polypeptides of three different types. It is composed of six dimeric outer subunits associated with a central cylindrical hexameric subunit through 12 biotinyl subunits; three outer subunits on each face of the centr...

journal_title：FEBS letters

authors： Thornton CG,Kumar GK,Shenoy BC,Haase FC,Phillips NF,Park VM,Magner WJ,Hejlik DP,Wood HG,Samols D

更新日期：1993-09-13 00:00:00

abstract：:Aging is accompanied by troubles resulting from changes in hormonal and nutritional status. Therefore, the abundance of mRNA coding for triiodothyronine (TR) and retinoic acid (RA) nuclear receptors was studied in the brain of young, adult and aged (2.5, 6 and 24 months, respectively) rats. In the brain of aged rats, ...

journal_title：FEBS letters

authors： Enderlin V,Alfos S,Pallet V,Garcin H,Azaïs-Braesco V,Jaffard R,Higueret P

更新日期：1997-08-04 00:00:00

abstract：:Previous studies of oxygen-derived free radical generation based on spin-trapping methods have shown a signal formed of six bands (sextet) using electron spin resonance spectrometry (ESR) of coronary effluents collected during post-ischaemic reperfusion of isolated hearts perfused with 5,5-dimethyl-1-pyrroline N-oxide...

journal_title：FEBS letters

authors： Boucher F,Pucheu S,Coudray C,Favier A,de Leiris J

更新日期：1992-05-18 00:00:00

abstract：:Determination of the protonation state of enzymes is a challenging problem in computational biophysics largely due to the vast number of possible protonic configurations. The protonation state dynamics of respiratory complex I was investigated via Monte Carlo and asynchronous dynamics simulations and a novel eigenvect...

journal_title：FEBS letters

authors： Couch V,Stuchebrukhov A

更新日期：2012-03-09 00:00:00

abstract：:A short form of Escherichia coli translational initiation factor IF3, repeatedly found both in vivo and in vitro, lacking the positively charged N-terminal hexapeptide has been produced by mild trypsinization. The properties of this short form of IF3 have been studied. Compared to the long native form of the factor, t...

journal_title：FEBS letters

authors： Lammi M,Pon CL,Gualerzi CO

更新日期：1987-05-04 00:00:00

abstract：:It was determined that flavan-3-ols and procyanidins have an inhibitory effect on angiotensin I converting enzyme (ACE) activity, and the effect was dependent on the number of epicatechin units forming the procyanidin. The inhibition by flavan-3-ols and procyanidins was competitive with the two substrates assayed: N-h...

journal_title：FEBS letters

authors： Actis-Goretta L,Ottaviani JI,Keen CL,Fraga CG

更新日期：2003-12-18 00:00:00

abstract：:Isolated mitotic chromosomes are able to form complexes with phosphatidylcholine liposomes in the presence and absence of Ca2+ ions, in the latter case in the presence of polyamines. Interactions with chromosomes stimulates liposome fusion. The fusion is promoted by condensed and EDTA-decondensed chromosomes. ...

journal_title：FEBS letters

authors： Balakireva LA,Belyaev ND,Budker VG,Kiseleva EV

更新日期：1991-06-24 00:00:00

abstract：:Peptide T, an octapeptide of sequence ASTTTNYT that binds to human T cells, was studied as a zwitterion in DMSOd6 solution by means of proton NMR spectroscopy at 500 MHz. The unusual dispersion of the resonances of residues of the same type (T) makes it possible to assign all resonances to specific residues by means o...

journal_title：FEBS letters

authors： Picone D,Temussi PA,Marastoni M,Tomatis R,Motta A

更新日期：1988-04-11 00:00:00

abstract：:NG-Methyl-L-arginine (L-NMA) and NG-nitro-L-arginine (L-NNA) inhibited NO-induced cGMP accumulation in porcine aortic endothelial cells with half-maximally effective concentrations of 15 and 3.4 microM, respectively. The effects of both compounds were reversible, but the L-NNA-induced inhibition was only reversed by w...

journal_title：FEBS letters

authors： Mayer B,Schmid M,Klatt P,Schmidt K

更新日期：1993-10-25 00:00:00

abstract：:Protein synthesis in cell-free systems of rat liver and kidney decreases markedly with age. Examination of activity changes of the different steps revealed for both types of organs that reduced binding of aminoacyl-tRNA to ribosomes and reduced peptidyl transfer might be of major importance for the decrease in overall...

journal_title：FEBS letters

authors： Gabius HJ,Engelhardt R,Deerberg F,Cramer F

更新日期：1983-08-22 00:00:00

abstract：:The photocycle of the light-adapted purple membrane was studied with a time-resolved fluorometry apparatus: fluorescence of the sample suspension (greater than 660 nm) was pumped with a 633-nm cw laser and the temporal change induced by a 532-nm pulsed laser was measured with a photon-counting-type transient recorder....

journal_title：FEBS letters

authors： Ohtani H,Itoh H,Shinmura T

更新日期：1992-06-22 00:00:00

abstract：:Steady-state kinetic analysis was performed on the reaction between D-fructose and ferricyanide with the quinohemoprotein fructose dehydrogenase from Gluconobacter species. The D-fructose oxidation dependence on the ferricyanide concentration resulted in a series of parallel reciprocal plots, and the reaction was assu...

journal_title：FEBS letters

authors： Marcinkeviciene J,Johansson G

更新日期：1993-02-22 00:00:00

abstract：:Phosphorylation of the 20 kDa myosin light chain from smooth muscle by five different kinases was investigated. Three of the kinases (myosin light chain kinase, phosphorylase kinase, and cAMP-dependent protein kinase) phosphorylate serine residues, the fourth (casein-kinase-2) mainly threonine, and the fifth (glycogen...

journal_title：FEBS letters

authors： Singh TJ,Akatsuka A,Huang KP

更新日期：1983-08-08 00:00:00

abstract：:The present study shows a direct impairing action of a cytotoxin-producing Helicobacter pylori strain on the Na+,K(+)-ATPase (evaluated as K(+)-dependent phosphatase activity) of human gastric epithelial cells in culture. The toxin itself is likely involved in this action which may also account for the cell edema foun...

journal_title：FEBS letters

authors： Ricci V,Sommi P,Cova E,Fiocca R,Romano M,Ivey KJ,Solcia E,Ventura U

更新日期：1993-11-15 00:00:00

abstract：:Three inositol-containing sialyloligosaccharides were isolated from normal human urine. Structural studies including gas-liquid chromatography of mono- and disaccharide derivatives, methylation analysis, mass spectrometry and glycosidase treatments indicated the structure NeuAc (alpha 2-3)Gal(beta 1-0)scyllo-inositol ...

journal_title：FEBS letters

authors： Parkkinen J

更新日期：1983-10-31 00:00:00

abstract：:Heterotrimeric G proteins were purified from bovine brain by immunoaffinity chromatography on immobilized anti G protein monoclonal antibody 3C2. Release of betagamma subunits was effectuated by exposure of immobilized trimeric G proteins to MgAlF4. The resultant betagamma subunits were pure and biologically active. F...

journal_title：FEBS letters

authors： Rehm A,Ploegh HL

更新日期：1997-02-03 00:00:00

abstract：:The efflux of [3H]purines from cultured sympathetic neurons prelabelled with [3H]adenine is accelerated 2-3-fold within hours of nerve growth factor (NGF) withdrawal and is reduced by readdition of NGF. Addition of 8-(4-chlorphenyl-thio) cAMP, which delays neurite degeneration, reduced the enhanced efflux of purines, ...

journal_title：FEBS letters

authors： Tolkovsky AM,Buckmaster EA

更新日期：1989-09-25 00:00:00

abstract：:The rotary motion in response to ATP hydrolysis of the ring of c subunits of the membrane portion, F(o), of ATP synthase, F(o)F(1), is still under contention. It was studied with EF(o)EF(1) (Escherichia coli) using microvideography with a fluorescent actin filament. To overcome the limited specificity of actin attachm...

journal_title：FEBS letters

authors： Pänke O,Gumbiowski K,Junge W,Engelbrecht S

更新日期：2000-04-21 00:00:00

abstract：:Fibromodulin and lumican are closely related members of the extracellular matrix leucine-rich repeat glycoprotein/proteoglycan family. Similar to decorin, another member of this protein family, they bind to fibrillar collagens and function in the assembly of the collagen network in connective tissues. We have studied ...

journal_title：FEBS letters

authors： Svensson L,Närlid I,Oldberg A

更新日期：2000-03-24 00:00:00

abstract：:There are numerous examples of how fundamental research has been required to understand and treat human disease. This article focuses on three human diseases of lipid metabolism in which advancements in understanding and treatment would not have been possible without basic research. Fabry disease is an inherited metab...

journal_title：FEBS letters

authors： Vance DE

更新日期：2006-10-09 00:00:00

abstract：:F-box proteins play pivotal roles in multiple cellular processes; however, little is known about their functions in glioma progression. In this study, we found that expression of the F-box and leucine-rich repeat protein 18 (FBXL18) is significantly upregulated in glioma tissues. Depletion of FBXL18 in glioma cells su...

journal_title：FEBS letters

authors： Zhang J,Yang Z,Ou J,Xia X,Zhi F,Cui J

更新日期：2017-01-01 00:00:00

abstract：:Coding region and intronic mutations in the gene for microtubule-associated protein tau cause frontotemporal dementia and Parkinsonism linked to chromosome 17 (FTDP-17). Most coding region mutations effect a reduced ability of tau protein to interact with microtubules and lead to the formation of a filamentous patholo...

journal_title：FEBS letters

authors： Smith MJ,Crowther RA,Goedert M

更新日期：2000-11-10 00:00:00

abstract：:We report here the first comprehensive comparative NH2-terminal sequence studies of interstitial retinol-binding protein (IRBPs) from nine mammals (including cattle) and one amphibian. This study has revealed that in many species the N-terminus of IRBP includes a 3-6 amino acid extension. IRBP possessing this leader s...

journal_title：FEBS letters

authors： Fong SL,Cook RG,Alvarez RA,Liou GI,Landers RA,Bridges CD

更新日期：1986-09-15 00:00:00

abstract：:Domain structures of porcine lung and testicular angiotensin-converting enzymes (ACE) were studied by radiation inactivation to test the hypothesis that lung ACE has two catalytic sites localized to discrete, structurally independent domains (the N- and C-domains) of approximately equal size. The minimum functional si...

journal_title：FEBS letters

authors： Sakaguchi H,Hirose S,Kume T,Hagiwara H

更新日期：1992-06-29 00:00:00