Functional regulation of reconstituted Na,K-ATPase by protein kinase A phosphorylation.


:Reconstituted Na+,K+-ATPase from either pig kidney or shark rectal glands was phosphorylated by cAMP dependent protein kinase, PKA. The stoichiometry was approximately 0.9 mol P(i)/mol alpha-subunit in the pig kidney enzyme and approximately 0.2 mol P(i)/mol alpha-subunit in the shark enzyme. In shark, Na+,K+-ATPase PKA phosphorylation increased the maximum hydrolytic activity for cytoplasmic Na+ activation and extracellular K+ activation without affecting the apparent K(m) values. In contrast, no significant functional effect after PKA phosphorylation was observed in pig kidney Na+,K+-ATPase.




FEBS letters


Cornelius F,Logvinenko N




Has Abstract


1996-02-19 00:00:00














  • The amino acid sequence of the zinc-requiring beta-lactamase II from the bacterium Bacillus cereus 569.

    abstract::The amino acid sequence of the zinc-requiring beta-lactamase II from Bacillus cereus strain 569 has been determined. It consists of a single polypeptide chain of 227 residues. It is the only example so far fully characterized of a class B beta-lactamase, and is structurally and mechanistically distinct from both the w...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ambler RP,Daniel M,Fleming J,Hermoso JM,Pang C,Waley SG

    更新日期:1985-09-23 00:00:00

  • A generic rate law for surface-active enzymes.

    abstract::Many biochemical reactions are confined to interfaces, such as membranes or cell walls. Despite their importance, no canonical rate laws describing the kinetics of surface-active enzymes exist. Combining the approach chosen by Michaelis and Menten 100 years ago with concepts from surface chemical physics, we here pres...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kartal O,Ebenhöh O

    更新日期:2013-09-02 00:00:00

  • PsbS enhances nonphotochemical fluorescence quenching in the absence of zeaxanthin.

    abstract::Leaves and chloroplasts from Arabidopsis plants with increased amounts of PsbS protein showed the same percentage increase in nonphotochemical quenching in comparison to the wild type both in the presence and absence of zeaxanthin. The absorption change at 525-535 nm was also more pronounced in both cases. It is sugge...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Crouchman S,Ruban A,Horton P

    更新日期:2006-04-03 00:00:00

  • NPC1L1 and cholesterol transport.

    abstract::The polytopic transmembrane protein, Niemann-Pick C1-Like 1 (NPC1L1), is enriched in the apical membrane of small intestine absorptive enterocytes where it mediates extracellular sterol transport across the brush border membrane. It is essential for intestinal sterol absorption and is the molecular target of ezetimibe...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Betters JL,Yu L

    更新日期:2010-07-02 00:00:00

  • Different epitopes are required for gp130 activation by interleukin-6, oncostatin M and leukemia inhibitory factor.

    abstract::Gp130 is the common signal transducing receptor subunit of interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor and cardiotrophin-1. IL-6 and IL-11 induce gp130 homodimerization whereas the others lead to the formation of heterodimers with LIFR or OSMR. Binding e...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Timmermann A,Pflanz S,Grötzinger J,Küster A,Kurth I,Pitard V,Heinrich PC,Müller-Newen G

    更新日期:2000-02-25 00:00:00

  • Structural and functional characterization of the bacterial translocation inhibitor GE82832.

    abstract::The structure of GE82832, a translocation inhibitor produced by a soil microorganism, is shown to be highly related to that of dityromycin, a bicyclodecadepsipeptide antibiotic discovered long ago whose characterization had never been pursued beyond its structural elucidation. GE82832 and dityromycin were shown to int...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Brandi L,Maffioli S,Donadio S,Quaglia F,Sette M,Milón P,Gualerzi CO,Fabbretti A

    更新日期:2012-09-21 00:00:00

  • Malarial toxic antigens synergistically enhance insulin signalling.

    abstract::Hypoglycaemia is a major complication of severe malaria [(1990) Trans. Roy. Soc. Trop. Med. 84 (suppl. 2) 1-65], especially cerebral malaria, in which it is associated with increased mortality [(1990) Lancet 336, 1039-1043; (1989) Quart. J. Med. (New series) 71, 441-459]; however, the mechanisms responsible have not b...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Taylor K,Carr R,Playfair JH,Saggerson ED

    更新日期:1992-10-26 00:00:00

  • The pro-phenoloxidase of coleopteran insect, Tenebrio molitor, larvae was activated during cell clump/cell adhesion of insect cellular defense reactions.

    abstract::To characterize the proteins involved in cell clump/cell adhesion of insect cellular defense reactions, we induced the cell clump/cell adhesion reaction in vitro with the hemolymph of larvae of the coleopteran insect, Tenebrio molitor. The 72 kDa protein was specifically enriched in the residues of cell clump/cell adh...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lee HS,Cho MY,Lee KM,Kwon TH,Homma K,Natori S,Lee BL

    更新日期:1999-02-12 00:00:00

  • Mesdc2 plays a key role in cell-surface expression of Lrp4 and postsynaptic specialization in myotubes.

    abstract::Low-density lipoprotein receptor-related protein 4 (Lrp4) is essential for pre- and post-synaptic specialization at the neuromuscular junction (NMJ), an indispensable synapse between a motor nerve and skeletal muscle. Muscle-specific receptor tyrosine kinase MuSK must form a complex with Lrp4 to organize postsynaptic ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hoshi T,Tezuka T,Yokoyama K,Iemura S,Natsume T,Yamanashi Y

    更新日期:2013-11-29 00:00:00

  • Traffic of prion protein between different compartments on the neuronal surface, and the propagation of prion disease.

    abstract::The key mechanism in prion disease is the conversion of cellular prion protein into an altered, pathogenic conformation, in which cellular mechanisms play a poorly understood role. Both forms of prion protein are lipid-anchored and reside in rafts that appear to protect the native conformation against conversion. Neur...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Morris RJ,Parkyn CJ,Jen A

    更新日期:2006-10-09 00:00:00

  • EGF induces efficient Cx43 gap junction endocytosis in mouse embryonic stem cell colonies via phosphorylation of Ser262, Ser279/282, and Ser368.

    abstract::Gap junctions (GJs) traverse apposing membranes of neighboring cells to mediate intercellular communication by passive diffusion of signaling molecules. We have shown previously that cells endocytose GJs utilizing the clathrin machinery. Endocytosis generates cytoplasmic double-membrane vesicles termed annular gap jun...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fong JT,Nimlamool W,Falk MM

    更新日期:2014-03-03 00:00:00

  • Promiscuous actions of small molecule inhibitors of the protein kinase D-class IIa HDAC axis in striated muscle.

    abstract::PKD-mediated phosphorylation of class IIa HDACs frees the MEF2 transcription factor to activate genes that govern muscle differentiation and growth. Studies of the regulation and function of this signaling axis have involved MC1568 and Gö-6976, which are small molecule inhibitors of class IIa HDAC and PKD catalytic ac...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lemon DD,Harrison BC,Horn TR,Stratton MS,Ferguson BS,Wempe MF,McKinsey TA

    更新日期:2015-04-28 00:00:00

  • Sirtuin-mediated deacetylation pathway stabilizes Werner syndrome protein.

    abstract::Caloric restriction (CR) is known to promote longevity in various species. Sirtuin-mediated deacetylation has been shown to be related to the promotion of longevity in some species. Here, we show that CR of rats led to an increase in the level of Werner syndrome protein (WRN), a recognized DNA repair protein. In addit...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kahyo T,Mostoslavsky R,Goto M,Setou M

    更新日期:2008-07-23 00:00:00

  • Involvement of soluble proteinous factors in auxin-induced modulation of P-type ATPase in rice (Oryza sativa L.) seedlings.

    abstract::The marked difference in auxin sensitivity between plant roots and shoots was studied in terms of auxin-induced stimulation of membrane P-type ATPase. The results suggest the existence in rice seedlings of, at least, two isoforms of soluble proteinous factors (SPF), SPF(I) and SPF(II), which are involved in the stimul...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kim D,Kim YS,Jung J

    更新日期:1997-06-09 00:00:00

  • Insulin activates protein kinase B, inhibits glycogen synthase kinase-3 and activates glycogen synthase by rapamycin-insensitive pathways in skeletal muscle and adipose tissue.

    abstract::Insulin stimulated protein kinase B alpha (PKB alpha) more than 10-fold and decreased glycogen synthase kinase-3 (GSK3) activity by 50 +/- 10% in skeletal muscle and adipocytes. Rapamycin did not prevent the activation of PKB, inhibition of GSK3 or stimulation of glycogen synthase up to 5 min. Thus rapamycin-insensiti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cross DA,Watt PW,Shaw M,van der Kaay J,Downes CP,Holder JC,Cohen P

    更新日期:1997-04-07 00:00:00

  • Curved or linear? Predicting the 3-dimensional structure of α-helical antimicrobial peptides in an amphipathic environment.

    abstract::α-Helical membrane-active antimicrobial peptides (AMPs) are known to act via a range of mechanisms, including the formation of barrel-stave and toroidal pores and the micellisation of the membrane (carpet mechanism). Different mechanisms imply that the peptides adopt different 3D structures when bound at the water-mem...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: van den Bergen G,Stroet M,Caron B,Poger D,Mark AE

    更新日期:2020-03-01 00:00:00

  • Mutations in subunit 6 of the F1F0-ATP synthase cause two entirely different diseases.

    abstract::A lowered efficiency of oxidative phosphorylation was recently found in a Leber hereditary optic neuropathy (LHON) proband carrying a mutation in the mtDNA gene for subunit 6 of the membrane-bound F0 segment of the F1F0-ATP synthase [9]. This phenotype was transferred to cytoplasmic hybrid cells together with the muta...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Majander A,Lamminen T,Juvonen V,Aula P,Nikoskelainen E,Savontaus ML,Wikström M

    更新日期:1997-07-28 00:00:00

  • An evaluation of the evidence for H+ pumping by reconstituted cytochrome c oxidase in the light of recent criticism.

    abstract::We review the evidence for H+ translocation by reconstituted cytochrome c oxidase; attention is paid to those aspects which we feel most open to criticism. Possible alternative hypotheses are assessed, with regard to experiments carried out to test them directly and with regard to published data. We conclude that, whi...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Casey RP,Azzi A

    更新日期:1983-04-18 00:00:00

  • Cell type specific localization of sphingomyelin biosynthesis.

    abstract::We have studied the incorporation of [(14)C]serine and of [(3)H]sphingosine into sphingomyelin in the presence or absence of brefeldin A (BFA) in three different cell types. Administration of BFA (1 microgram/ml) to fibroblasts for 24 h increased the incorporation of label into sphingomyelin 1.5-3 fold compared with u...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sadeghlar F,Sandhoff K,van Echten-Deckert G

    更新日期:2000-07-28 00:00:00

  • A novel heterodimeric antimicrobial peptide from the tree-frog Phyllomedusa distincta.

    abstract::We present here the purification and the analysis of the structural and functional properties of distinctin, a 5.4 kDa heterodimeric peptide with antimicrobial activity from the tree-frog Phyllomedusa distincta. This peptide was isolated from the crude extract of skin granular glands by different chromatographic steps...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Batista CV,Scaloni A,Rigden DJ,Silva LR,Rodrigues Romero A,Dukor R,Sebben A,Talamo F,Bloch C

    更新日期:2001-04-06 00:00:00

  • Primary structure of sheep prostaglandin endoperoxide synthase deduced from cDNA sequence.

    abstract::The complete amino acid sequence of prostaglandin endoperoxide synthase from sheep vesicular gland has been deduced by cloning and sequence analysis of DNA complementary to its messenger RNA. The results were confirmed by digestion of the enzyme with carboxypeptidase Y and by automated Edman degradation of the intact ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yokoyama C,Takai T,Tanabe T

    更新日期:1988-04-25 00:00:00

  • A conjugate of the A1 peptide of cholera toxin and the lectin of Wisteria floribunda that activates the adenylate cyclase of intact cells.

    abstract::The active A1 peptide of cholera toxin was linked by a disulphide bond to the lectin of Wisteria floribunda. The resulting conjugate activated the adenylate cyclase of intact U937 or K562 cells at the same concentrations as native toxin did, but to a greater extent. Activation was inhibited by N-acetyl-D-galactosamine...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: van Heyningen S

    更新日期:1983-11-28 00:00:00

  • The crystal structure of the amidohydrolase VinJ shows a unique hydrophobic tunnel for its interaction with polyketide substrates.

    abstract::VinJ is an amidohydrolase belonging to the serine peptidase family that catalyzes the hydrolysis of the terminal aminoacyl moiety of a polyketide intermediate during the biosynthesis of vicenistatin. Herein, we report the crystal structure of VinJ. VinJ possesses a unique hydrophobic tunnel for the recognition of the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Shinohara Y,Miyanaga A,Kudo F,Eguchi T

    更新日期:2014-03-18 00:00:00

  • Identification of a troponin-I like protein in platelet preparations as histone H2B.

    abstract::A tropomyosin-binding protein (app. Mr 17000) was detected in equine platelet preparations by a gel overlay technique. Its isolation, amino acid and partial sequence analyses have shown it to be histone H2B. As with a similar protein from pig platelet preparations [der Terrossian et al. (1983) FEBS Lett. 152, 202-206]...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Stewart DI,Golosinska K,Smillie LB

    更新日期:1983-06-27 00:00:00

  • Inhibition of ADP-ribosylation suppresses aberrant accumulation of lipidated apolipoprotein B in the endoplasmic reticulum.

    abstract::ApoB-crescent, an endoplasmic reticulum (ER)-lipid droplet amalgamation structure, is a useful marker to indicate aberrant lipidated apolipoprotein B accumulation in the hepatocyte ER. Blockade of the ER-to-Golgi transport by either vesicle transport inhibitors or dominant-negative Arf1 caused a significant increase i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ohsaki Y,Cheng J,Yamairi K,Pan X,Hussain MM,Fujimoto T

    更新日期:2013-11-15 00:00:00

  • TeloPCR-seq: a high-throughput sequencing approach for telomeres.

    abstract::We have developed a high-throughput sequencing approach that enables us to determine terminal telomere sequences from tens of thousands of individual Schizosaccharomyces pombe telomeres. This method provides unprecedented coverage of telomeric sequence complexity in fission yeast. S. pombe telomeres are composed of mo...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Bennett HW,Liu N,Hu Y,King MC

    更新日期:2016-12-01 00:00:00

  • Amino acid substitutions contributing to α2,6-sialic acid linkage binding specificity of human parainfluenza virus type 3 hemagglutinin-neuraminidase.

    abstract::Human parainfluenza virus type 3 (hPIV3) recognizes both α2,3- and α2,6-linked sialic acids, whereas human parainfluenza virus type 1 (hPIV1) recognizes only α2,3-linked sialic acids. To identify amino acid residues that confer α2,6-linked sialic acid recognition of hPIV3, amino acid residues in or neighboring the sia...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fukushima K,Takahashi T,Ueyama H,Takaguchi M,Ito S,Oishi K,Minami A,Ishitsubo E,Tokiwa H,Takimoto T,Suzuki T

    更新日期:2015-05-08 00:00:00

  • A factor that specifically binds to the 5'-untranslated region of encephalomyocarditis virus RNA.

    abstract::A protein factor that specifically binds to the 5'-untranslated region of encephalomyocarditis virus (EMCV) RNA has been found in extracts of ascites carcinoma Krebs-2 cells. This was done using UV-irradiation on extracts supplemented with in vitro synthesized 32P-labelled transcripts followed by analysis of crosslink...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Borovjagin AV,Evstafieva AG,Ugarova TYu,Shatsky IN

    更新日期:1990-02-26 00:00:00

  • Cell cycle-dependent association of Gag-Mil and hsp90.

    abstract::Immunoprecipitated p100Gag-Mil protein kinase from MH2-transformed quail embryo fibroblasts is associated with an 89 kDa protein. The molar ratio between p89 and Gag-Mil in the immunocomplex is 0.72, indicating that the majority of Gag-Mil is complexed with p89. During mitosis part of Gag-Mil is shifted to a form with...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lovrić J,Bischof O,Moelling K

    更新日期:1994-04-18 00:00:00

  • A novel human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, GalNAc-T7, with specificity for partial GalNAc-glycosylated acceptor substrates.

    abstract::A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase gene family, designated GalNAc-T7, was cloned and expressed. GalNAc-T7 exhibited different properties compared to other characterized members of this gene family, in showing apparent exclusive specificity for partial...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bennett EP,Hassan H,Hollingsworth MA,Clausen H

    更新日期:1999-10-29 00:00:00