Functional regulation of reconstituted Na,K-ATPase by protein kinase A phosphorylation.


:Reconstituted Na+,K+-ATPase from either pig kidney or shark rectal glands was phosphorylated by cAMP dependent protein kinase, PKA. The stoichiometry was approximately 0.9 mol P(i)/mol alpha-subunit in the pig kidney enzyme and approximately 0.2 mol P(i)/mol alpha-subunit in the shark enzyme. In shark, Na+,K+-ATPase PKA phosphorylation increased the maximum hydrolytic activity for cytoplasmic Na+ activation and extracellular K+ activation without affecting the apparent K(m) values. In contrast, no significant functional effect after PKA phosphorylation was observed in pig kidney Na+,K+-ATPase.




FEBS letters


Cornelius F,Logvinenko N




Has Abstract


1996-02-19 00:00:00














  • Voltage-gated K+ currents of mouse dendritic cells.

    abstract::Dendritic cells (DC) were enriched from murine spleen by exploring their intermediate density and transient weak adherence. The isolated population contained excellent antigen presenting cells with high surface expression of major histocompatibility complex (MHC) class II determinants thus exhibiting crucial immunofun...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fischer HG,Eder C

    更新日期:1995-10-09 00:00:00

  • Functional proteomics of circadian expressed proteins from Chlamydomonas reinhardtii.

    abstract::In this study, functional proteomics was successfully applied for the characterization of circadian expressed, basic proteins. For this purpose, we have chosen the green model alga Chlamydomonas reinhardtii since its entire nuclear genome is available and it is ideally suited for biochemical enrichment procedures. Pro...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wagner V,Fiedler M,Markert C,Hippler M,Mittag M

    更新日期:2004-02-13 00:00:00

  • Thiazolidinedione inhibits production of RANTES in a cytokine-treated human lung epithelial cell line.

    abstract::The chemokine RANTES is a potent chemoattractant for eosinophils. RANTES is produced by lung epithelial cells during eosinophil-rich inflammatory diseases such as asthma. In this study, we examined the effects of thiazolidinediones (TZD) on RANTES expression in a human lung epithelial cell line, A549. In A549 cells, i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Momoi A,Murao K,Imachi H,Sayo Y,Nakamura H,Hosokawa H,Sato M,Fujita J,Okada H,Ishida T,Takahara J

    更新日期:1999-06-11 00:00:00

  • Peptidyl-prolyl cis-trans isomerase activity as studied by dynamic proton NMR spectroscopy.

    abstract::Recently the identity of the peptidyl-prolyl cis-trans isomerase (PPIase), which accelerates the cis/trans isomerization of prolyl peptide bonds and cyclophilin, the binding protein for the immunosuppressive drug Cyclosporin A (CsA), was discovered. The PPIase catalysis toward the substrate Suc-Ala-Phe-Pro-Phe-pNA has...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hübner D,Drakenberg T,Forsén S,Fischer G

    更新日期:1991-06-17 00:00:00

  • Inhibition of the replication of native and 3'-azido-2',3'-dideoxythymidine (AZT)-resistant simian immunodeficiency virus (SIV) by 3-nitrosobenzamide.

    abstract::The 3-nitrosobenzamide (NOBA) drug abolishes SIV replication sharply at 20 microM concentration when CEM x 174 cells are preincubated for 1 h with the drug prior to viral infection. Treatment of CEM x 174 cells with 20 microM NOBA resulted in the inhibition of the synthesis of the DNA sequence coding for the gag gene,...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chuang AJ,Killam KF Jr,Chuang RY,Rice WG,Schaeffer CA,Mendeleyev J,Kun E

    更新日期:1993-07-12 00:00:00

  • The p53-inducible E3 ubiquitin ligase p53RFP induces p53-dependent apoptosis.

    abstract::Recently, it has been shown that really interesting new gene (RING)-in between ring finger (IBR)-RING domain-containing proteins, such as Parkin and Parc, are E3 ubiquitin ligases and are involved in regulation of apoptosis. In this report, we show that p53-inducible RING-finger protein (p53RFP), a p53-inducible E3 ub...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Huang J,Xu LG,Liu T,Zhai Z,Shu HB

    更新日期:2006-02-06 00:00:00

  • Are there isoenzymes of cytochrome c oxidase in Paracoccus denitrificans?

    abstract::We have used a gene replacement strategy to delete the previously isolated gene [(1987) EMBO J. 6, 2825-2833] for the cytochrome c oxidase subunit I from Paracoccus denitrificans. The resulting mutant was still able to synthesize active cytochrome c oxidase. This led us to look for another locus which could completely...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Raitio M,Pispa JM,Metso T,Saraste M

    更新日期:1990-02-26 00:00:00

  • Uncoupling protein UCP2: when mitochondrial activity meets immunity.

    abstract::Uncoupling protein 2 (UCP2) belongs to the family of mitochondrial carriers. Here, we highlight recent findings regarding UCP2 function in the immune system. UCP2 controls immune cell activation by modulating MAPK pathways and the production of mitochondrial reactive oxygen species. In several models of infection, inf...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Emre Y,Nübel T

    更新日期:2010-04-16 00:00:00

  • The common C-terminal sequences of substance P and neurokinin A contact the same region of the NK-1 receptor.

    abstract::Although neurokinin A (NKA), a tachykinin peptide with sequence homology to substance P (SP), is a weak competitor of radiolabeled SP binding to the NK-1 receptor (NK-1R), more recent direct binding studies using radiolabeled NKA have demonstrated an unexpected high-affinity interaction with this receptor. To document...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bremer AA,Leeman SE,Boyd ND

    更新日期:2000-12-01 00:00:00

  • Functional and molecular characterization of a peptide transporter in the rat PC12 neuroendocrine cell line.

    abstract::We have studied functional properties of peptide transport in the pheochromocytoma neuroendocrine cell line from rat. The neutral peptide D-Phe-L-Ala (resistant to hydrolysis) is a good substrate for uptake into these cells. Transport is substantially inhibited by diethylpyrocarbonate pretreatment and is stimulated by...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hussain I,Zanic-Grubisic T,Kudo Y,Boyd CA

    更新日期:2001-11-23 00:00:00

  • The 18S ribosomal RNA sequence of the sea anemone Anemonia sulcata and its evolutionary position among other eukaryotes.

    abstract::Evolutionary trees based on partial small ribosomal subunit RNA sequences of 22 metazoa species have been published [(1988) Science 239, 748-753]. In these trees, cnidarians (Radiata) seemed to have evolved independently from the Bilateria, which is in contradiction with the general evolutionary view. In order to furt...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hendriks L,Van de Peer Y,Van Herck M,Neefs JM,De Wachter R

    更新日期:1990-09-03 00:00:00

  • TeloPCR-seq: a high-throughput sequencing approach for telomeres.

    abstract::We have developed a high-throughput sequencing approach that enables us to determine terminal telomere sequences from tens of thousands of individual Schizosaccharomyces pombe telomeres. This method provides unprecedented coverage of telomeric sequence complexity in fission yeast. S. pombe telomeres are composed of mo...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Bennett HW,Liu N,Hu Y,King MC

    更新日期:2016-12-01 00:00:00

  • Enhancement by streptozotocin of O2- radical generation by the xanthine oxidase system of pancreatic beta-cells.

    abstract::Spin-trapping techniques and electron spin resonance (ESR) spectroscopy were used to study the relationship between the effect of streptozotocin (STZ) on pancreatic beta-cells and free radical formation by these cells. Results showed that STZ enhanced generation of the DMPO-OH radical adduct, which is a degradation pr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nukatsuka M,Sakurai H,Yoshimura Y,Nishida M,Kawada J

    更新日期:1988-11-07 00:00:00

  • Protein transport via the cpTat pathway displays cooperativity and is stimulated by transport-incompetent substrate.

    abstract::Kinetic analyses of cpTat-mediated protein transport across the thylakoid membrane were conducted, revealing three important characteristics of this translocation pathway. First, transport via the cpTAT system displays a non-Michaelis-Menten, sigmoidal rate-substrate relationship with an apparent Hill coefficient of 1...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Alder NN,Theg SM

    更新日期:2003-04-10 00:00:00

  • Biochemical characterization of the p51 sub-unit of human immunodeficiency virus reverse transcriptase in homo- and heterodimeric recombinant forms of the enzyme.

    abstract::The biochemical properties of the p51 subunit of HIV-1 reverse transcriptase (RT) were studied in order to understand its role in the heterodimeric form p66/p51 found in virions. A recombinant form of RT, p51/p51, expressed in yeast, was purified and characterized. The enzyme was affinity labeled using a 5' modified o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: el Dirani-Diab R,Andreola ML,Nevinsky G,Tharaud D,Barr PJ,Litvak S,Tarrago-Litvak L

    更新日期:1992-04-13 00:00:00

  • The design of an alternative, covalently flavinylated 6-hydroxy-D-nicotine oxidase by replacing the FAD-binding histidine by cysteine and reconstitution of the holoenzyme with 8-(methylsulfonyl)FAD.

    abstract::The cofactor of several flavoenzymes is autocatalytically bound to the polypeptide via a histidyl(N3)-(8alpha)-FAD linkage which makes the generation of apoenzyme difficult. We introduced an alternative covalent protein-FAD bond at the active site of 6-hydroxy-D-nicotine oxidase (6HDNO) by replacing the FAD-binding hi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Stoltz M,Henninger HP,Brandsch R

    更新日期:1996-05-20 00:00:00

  • Cryogenic absorption spectra of hydroperoxo-ferric heme oxygenase, the active intermediate of enzymatic heme oxygenation.

    abstract::Using radiolysis with (32)P enriched phosphate as an internal source of ionizing radiation, the formation of hydroperoxo-ferric complex from oxy-ferrous precursor with a high yield was monitored at 77 K in heme oxygenase (HO) by means of optical absorption spectroscopy. Well-resolved absorption spectra (maxima at 421 ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Denisov IG,Ikeda-Saito M,Yoshida T,Sligar SG

    更新日期:2002-12-04 00:00:00

  • Studies on the synthesis and secretion of transthyretin by the human hepatoma cell line Hep G2.

    abstract::Transthyretin (TTR) is a circulatory protein which plays an important role in the transport of both thyroid hormone and retinol. Hep G2 cells, a human hepatoma-derived cell line, have been used extensively in studies of protein secretion by liver cells. The original description of this cell line indicated that this li...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Blaner WS,Bonifácio MJ,Feldman HD,Piantedosi R,Saraiva MJ

    更新日期:1991-08-05 00:00:00

  • Induction of nuclear factor kappaB and activation protein 1 in postischemic myocardium.

    abstract::Ischemia/reperfusion induces nuclear factor kappaB (NF-kappaB) and AP-1 in rat hearts after 15 min of ischemia followed by reperfusion (R) for various periods of time (15 and 30 min, 1, 2, 3, 6, 12, and 24 h). Low levels of NF-kappaB and no signal for AP-1 were detected in shams and in non-ischemic tissue distant from...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chandrasekar B,Freeman GL

    更新日期:1997-01-13 00:00:00

  • The nuclear-encoded 18 kDa (IP) AQDQ subunit of bovine heart complex I is phosphorylated by the mitochondrial cAMP-dependent protein kinase.

    abstract::In bovine heart mitochondria a protein of M(r) 18 kDa, phosphorylated by mtPKA, is associated to the NADH-ubiquinone oxidoreductase in the inner membrane and is present in purified preparation of this complex. The 18 kDa phosphoprotein has now been isolated and sequenced. It is identified as the 18 kDa (IP) AQDQ subun...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Papa S,Sardanelli AM,Cocco T,Speranza F,Scacco SC,Technikova-Dobrova Z

    更新日期:1996-02-05 00:00:00

  • The interplay between structure and function in intrinsically unstructured proteins.

    abstract::Intrinsically unstructured proteins (IUPs) are common in various proteomes and occupy a unique structural and functional niche in which function is directly linked to structural disorder. The evidence that these proteins exist without a well-defined folded structure in vitro is compelling, and justifies considering th...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Tompa P

    更新日期:2005-06-13 00:00:00

  • Rainbow trout ovarian cholesterol side-chain cleavage cytochrome P450 (P450scc). cDNA cloning and mRNA expression during oogenesis.

    abstract::A cDNA clone encoding cholesterol side-chain cleavage cytochrome P450 (P450scc) was isolated from a rainbow trout ovarian follicle cDNA library. The cDNA contains an open reading frame of 1,542 nucleotides encoding a protein of 514 amino acids. The predicted amino acid sequence of trout P450scc shows 48% homology with...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Takahashi M,Tanaka M,Sakai N,Adachi S,Miller WL,Nagahama Y

    更新日期:1993-03-15 00:00:00

  • Amino acids 225-235** of the protein C serine-protease domain are important for the interaction with the thrombin-thrombomodulin complex.

    abstract::Protein C (PC) is a vitamin K-dependent zymogen that inactivates factors Va and VIIIa after its activation by thrombin complexed to thrombomodulin. We characterized a monoclonal antibody (mAb) against PC, whose only influence on PC functions was to inhibit PC activation by the thrombin-thrombomodulin complex. It recog...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vincenot A,Gaussem P,Pittet JL,Debost S,Aiach M

    更新日期:1995-06-26 00:00:00

  • Isolation and characterisation of a functional alpha beta heterodimer from the ATP synthase of Rhodospirillum rubrum.

    abstract::An alpha beta heterodimer of the F1-ATPase of Rhodospirillum rubrum was isolated by extraction of chromatophores with LiCl. Each alpha beta heterodimer contains one tightly bound ADP, which is released upon removal of medium Mg2+. The dimer can be reversibly dissociated by removal of Mg(2+)-ions. The alpha beta hetero...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Andralojc PJ,Harris DA

    更新日期:1992-09-28 00:00:00

  • N-acetylcysteine suppresses TNF-induced NF-kappaB activation through inhibition of IkappaB kinases.

    abstract::Here, we used a reductant, N-acetyl-L-cysteine (NAC), to investigate the redox-sensitive step(s) in the signalling pathway from the tumor necrosis factor (TNF) receptor to nuclear factor kappaB (NF-kappaB). We found that NAC suppressed NF-kappaB activation triggered by TNF or by overexpression of either the TNF recept...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Oka S,Kamata H,Kamata K,Yagisawa H,Hirata H

    更新日期:2000-04-28 00:00:00

  • Promiscuity in the part-phosphorylative Entner-Doudoroff pathway of the archaeon Sulfolobus solfataricus.

    abstract::The hyperthermophilic archaeon Sulfolobus solfataricus metabolises glucose and galactose by a 'promiscuous' non-phosphorylative variant of the Entner-Doudoroff pathway, in which a series of enzymes have sufficient substrate promiscuity to permit the metabolism of both sugars. Recently, it has been proposed that the pa...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lamble HJ,Theodossis A,Milburn CC,Taylor GL,Bull SD,Hough DW,Danson MJ

    更新日期:2005-12-19 00:00:00

  • Binding of Ala-scanning analogs of omega-conotoxin MVIIC to N- and P/Q-type calcium channels.

    abstract::omega-Conotoxin MVIIC binds to P/Q-type calcium channels with high affinity and N-type channels with low affinity. To reveal the residues essential for subtype selectivity, we synthesized Ala-scanning analogs of MVIIC. Binding assays using rat cerebellar P(2) membranes suggested that Thr(11), Tyr(13) and Lys(2) are es...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sato K,Raymond C,Martin-Moutot N,Sasaki T,Ohtake A,Minami K,Van Renterghem C,Kim JI,Takahashi M,Seagar MJ

    更新日期:2000-03-10 00:00:00

  • Interaction between calmodulin and microtubule-associated proteins prepared at different stages of brain development.

    abstract::Rat brain microtubules were prepared at the adult stage and from immature (i.e., 4-day-old) animals. At an early stage of development, the composition of microtubule-associated proteins is qualitatively different from that found at the adult stage [(1982) Eur. J. Biochem. 129, 465-471]. The influence of calmodulin on ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Erneux C,Passareiro H,Nunez J

    更新日期:1984-07-09 00:00:00

  • Visualisation by electron microscopy of the unique part of the cytoplasmic domain of a desmoglein, a cadherin-like protein of the desmosome type of cell junction.

    abstract::Part of the cytoplasmic domain of a human desmoglein, Dsg1, a cadherin-like protein found in desmosomes of epithelial cells, has been visualised by electron microscopy. The cloned fragment contains five repeats of a 29 +/- 4 residue sequence unique to desmogleins, followed by a glycine-rich region. In rotary shadowed ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rutman AJ,Buxton RS,Burdett ID

    更新日期:1994-10-17 00:00:00

  • Functional relevance of the internal hydrophobic cavity of urate oxidase.

    abstract::Urate oxidase from Aspergillus flavus is a 135 kDa homo-tetramer which has a hydrophobic cavity buried within each monomer and located close to its active site. Crystallographic studies under moderate gas pressure and high hydrostatic pressure have shown that both gas presence and high pressure would rigidify the cavi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Colloc'h N,Prangé T

    更新日期:2014-05-02 00:00:00