Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid-Blastula Transition and induces apoptosis in zebrafish.

abstract：:Rab5 GTPases are master regulators of early endosome biogenesis and transport. The genome of Saccharomyces cerevisiae encodes three Rab5 proteins: Vps21, the major isoform, Ypt52 and Ypt53. Here, we show that Vps21 is the most abundant Rab5 protein and Ypt53 is the least abundant. In stressed cells, Ypt53 levels incre...

journal_title：FEBS letters

authors： Schmidt O,Weyer Y,Fink MJ,Müller M,Weys S,Bindreither M,Teis D

更新日期：2017-09-01 00:00:00

abstract：:RNA-binding protein kinase from amphibian oocytes modifies serine and threonine residues in the molecules of substrates and utilizes both ATP and GTP. Low concentrations of heparin inhibit protein kinase. The foregoing suggests that this enzyme is casein kinase II. It is shown that RNA-binding proteins lack active for...

journal_title：FEBS letters

authors： Kandror KV,Stepanov AS

更新日期：1984-05-07 00:00:00

abstract：:A cDNA clone encoding cytochrome P-450(11)beta of rat adrenal has been cloned and sequenced using a bovine P-450(11)beta cDNA insert (pcP-450(11 beta)-2; (1987) J. Biochem. 102, 559-568) as a probe. The nucleotide sequence contains an open reading frame sufficient to encode the entire amino acid sequence of a P-450(11...

journal_title：FEBS letters

authors： Nonaka Y,Matsukawa N,Morohashi,Omura T,Ogihara T,Teraoka H,Okamoto M

更新日期：1989-09-11 00:00:00

abstract：:We have measured SGLT1 mRNA content and SGLT1-mediated glucose transport at different positions along the small intestine of control and streptozotocin diabetic rats and shown both parameters to be similar but higher in jejunal compared with ileal tissue. No such correlation was seen when comparing measurements of SGL...

journal_title：FEBS letters

authors： Dong R,Srai SK,Debnam E,Smith M

更新日期：1997-04-07 00:00:00

abstract：:Recently, we cloned several fluorescent proteins of different colors homologous to Aequorea victoria green fluorescent protein, which have great biotechnological potential as in vivo markers of gene expression. However, later investigations revealed severe drawbacks in the use of novel fluorescent proteins (FPs), in p...

journal_title：FEBS letters

authors： Yanushevich YG,Staroverov DB,Savitsky AP,Fradkov AF,Gurskaya NG,Bulina ME,Lukyanov KA,Lukyanov SA

更新日期：2002-01-30 00:00:00

abstract：:The matrix protein M of Borna disease virus (BDV) is a constituent of the viral envelope covering the inner leaflet of the lipid bilayer. BDV-M was expressed as recombinant protein in Escherichia coli, purified to homogeneity and structurally analyzed. Recombinant M (i) forms non-covalently bound multimers with a Stok...

journal_title：FEBS letters

authors： Kraus I,Bogner E,Lilie H,Eickmann M,Garten W

更新日期：2005-05-09 00:00:00

abstract：:The delta psi of R. sphaeroides, grown under high light to reduce the levels of light-harvesting bacteriochlorophyll, was naturally manipulated using light intensity. The relationship between delta psi and the swimming speed of free swimming populations of cells was investigated. After de-energisation by incubation in...

journal_title：FEBS letters

authors： Harrison DM,Packer HL,Armitage JP

更新日期：1994-07-04 00:00:00

abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV) ORF57 plays a pivotal role in mediating the nuclear export of KSHV intronless transcripts. We demonstrate that ORF57 localises and dynamically trafficks through the nucleolus. To assess if nucleolar shuttling of ORF57 is important for the export of intronless viral mRNA, ...

journal_title：FEBS letters

authors： Boyne JR,Whitehouse A

更新日期：2009-11-19 00:00:00

abstract：:Complexes between the urokinase-type plasminogen activator (uPA) and its type-2 inhibitor (PAI-2) are bound by a cell-surface receptor for uPA and rapidly cleaved into two fragments of 70 and 22 kDa. The 70-kDa fragment contains the active site of uPA and PAI-2, while the 22-kDa species was identified as the amino ter...

journal_title：FEBS letters

authors： Ragno P,Montuori N,Vassalli JD,Rossi G

更新日期：1993-06-01 00:00:00

abstract：:Fourier transform infrared spectroscopy is used to estimate the secondary structure of bacteriorhodopsin dissolved in chloroform-methanol (1:1 v/v), 0.1 M LiClO4. Curve-fitting of the deconvolved spectra in the amide I region shows that the total content of alpha-helices, reverse turns and beta-sheets are similar to t...

journal_title：FEBS letters

authors： Torres J,Padrós E

更新日期：1993-02-22 00:00:00

abstract：:A membrane-bound phospholipase C-like hydrolase present in lysates of bloodstream forms of Trypanosoma brucei rapidly converts the membrane form of the variant surface protein to the soluble form and 1,2-dimyristoylglycerol [(1985) M.A.J. Ferguson et al. J. Biol. Chem., 260, 4963-4968]. The hydrolase is inhibited by p...

journal_title：FEBS letters

authors： Bülow R,Overath P

更新日期：1985-07-22 00:00:00

abstract：:The effects of cold exposure and T4 administration on fructose 2,6-bisphosphate levels, phosphofructokinase-2 and pyruvate kinase activities were examined in rat brown adipose tissue. Cold adaptation (14 days) gave rise to a 2-fold increase in the amount of fructose 2,6-bisphosphate and phosphofructokinase-2 activity,...

journal_title：FEBS letters

authors： Sobrino F,Gualberto A,Pintado E

更新日期：1988-02-29 00:00:00

abstract：:Three clones were isolated from a lobster digestive gland cDNA library, using oligonucleotide probes based on the partial amino terminal sequence of a digestive cysteine proteinase. The cDNAs, LCP1, LCP2 and LCP3 encode preproenzymes of 322, 323 and 321 amino acid residues, and putative mature enzymes of 217, 216 and ...

journal_title：FEBS letters

authors： Laycock MV,MacKay RM,Di Fruscio M,Gallant JW

更新日期：1991-11-04 00:00:00

abstract：:The purpose of this investigation was to study the effect on cap structure for trans-splicing in mammalian cells. The early SV40 Bst/Bam pre-mRNA (cRNA) was synthesized in vitro in both capped (cap-Bst/Bam-cRNA) and non-capped (Bst/Bam-cRNA) versions and microinjected into the nuclei of TC7 cells. Trans-splicing was m...

journal_title：FEBS letters

authors： Graessmann M,Eul J,Berg B,Zimmermann C,Graessmann A

更新日期：1996-09-30 00:00:00

abstract：:As an attempt to investigate the dynamic interactions between plasma serine protease, coagulation factor VIIa (VIIa) and its cofactor, tissue factor (TF), we performed normal mode analysis (NMA) of the complex of VIIa with soluble TF (the extracellular part of TF; sTF). We compared fluctuations of Calpha atoms of VIIa...

journal_title：FEBS letters

authors： Soejima K,Kurihara Y,Kamiya K,Umeyama H

更新日期：1999-12-10 00:00:00

abstract：:Chromogranin A is known to undergo pH induced conformational changes, and the difference in conformation is supposed to be responsible for the difference in Ca2+ binding property. To gain insight regarding the overall structure and the nature of pH-induced conformational changes of chromogranin A, limited trypsin dige...

journal_title：FEBS letters

authors： Yoo SH,Ferretti JA

更新日期：1993-11-22 00:00:00

abstract：:Nitric oxide, as well as being a major regulator of vascular reactivity, has been shown to be one of the mediators of cytotoxicity in macrophages. This cytotoxic effect seems to be due to the interaction between nitric oxide and oxygen-related free radicals. This study shows that, in vitro, nitric oxide reacts with hy...

journal_title：FEBS letters

authors： Noronha-Dutra AA,Epperlein MM,Woolf N

更新日期：1993-04-19 00:00:00

abstract：:Cyclic AMP-mediated phosphorylation of calcium channel subunits was studied in vitro and in vivo in preparations from dog heart. Calcium channels in native cardiac membranes were phosphorylated by cAMP-dependent protein kinase (PKA) solubilized with digitonin and subsequently immunoprecipitated using a polyclonal anti...

journal_title：FEBS letters

authors： Haase H,Karczewski P,Beckert R,Krause EG

更新日期：1993-12-06 00:00:00

abstract：:Liposomes can be loaded with weak acids and bases, which exist in solutions in equilibrium with membrane permeable uncharged form, using various gradients across their membranes. Because in some cases the estimated drug concentration in the loaded liposomes exceeds their aqueous solubility we investigated the physical...

journal_title：FEBS letters

authors： Lasic DD,Frederik PM,Stuart MC,Barenholz Y,McIntosh TJ

更新日期：1992-11-09 00:00:00

abstract：:Jun activation domain-binding protein 1 (Jab1) is a coactivator of activating protein-1 (AP-1) and is the fifth component of the COP9 signalosome complex. It interacts with a variety of proteins and plays important roles in diverse signaling pathways and cellular function including oncogenesis. We show here that Jab1 ...

journal_title：FEBS letters

authors： Wang H,Sun X,Luo Y,Lin Z,Wu J

更新日期：2006-10-30 00:00:00

abstract：:We report here the regulation of the biosynthesis and the secretion of C-type natriuretic peptide (CNP) in cultured bovine chromaffin cells. The combined treatment with protein kinase A and -C activators induced a 6-fold increase of intracellular levels of CNP-(1-103). The biosynthesized CNP-(1-103) was co-released wi...

journal_title：FEBS letters

authors： Babinski K,Féthière J,Roy M,De Léan A,Ong H

更新日期：1992-11-30 00:00:00

abstract：:Glutathione peroxidase 3 (GPX3) is an important member of antioxidant enzymes for reducing reactive oxygen species and maintaining the oxygen balance. Gpx3 mRNA is strongly expressed in decidual cells from days 5 to 8 of pregnancy. After pregnant mice are treated with GPX inhibitor for 3 days, pregnancy rate is signif...

journal_title：FEBS letters

authors： Xu X,Leng JY,Gao F,Zhao ZA,Deng WB,Liang XH,Zhang YJ,Zhang ZR,Li M,Sha AG,Yang ZM

更新日期：2014-05-02 00:00:00

abstract：:We have studied functional properties of peptide transport in the pheochromocytoma neuroendocrine cell line from rat. The neutral peptide D-Phe-L-Ala (resistant to hydrolysis) is a good substrate for uptake into these cells. Transport is substantially inhibited by diethylpyrocarbonate pretreatment and is stimulated by...

journal_title：FEBS letters

authors： Hussain I,Zanic-Grubisic T,Kudo Y,Boyd CA

更新日期：2001-11-23 00:00:00

abstract：:A native female-specific chemoreceptive protein of a swallowtail butterfly [oviposition stimulant binding protein (OSBP)] was shown to specifically bind to aristolochic acid, a main stimulant for oviposition from its host plant. Oviposition stimulants are recognized by chemoreceptive organs of insects. OSBP isolated p...

journal_title：FEBS letters

authors： Tsuchihara K,Wazawa T,Ishii Y,Yanagida T,Nishida R,Zheng XG,Ishiguro M,Yoshihara K,Hisatomi O,Tokunaga F

更新日期：2009-01-22 00:00:00

abstract：:The scorpion toxin peptide BeKm-1 was synthesised by fluorenylmethoxycarbonyl solid phase chemistry and folded by air oxidation. The peptide's effects on heterologous human ether-a-go-go-related gene potassium current (I(HERG)) in HEK293 cells were assessed using 'whole-cell' patch clamp. Blockade of I(HERG) by BeKm-1...

journal_title：FEBS letters

authors： Milnes JT,Dempsey CE,Ridley JM,Crociani O,Arcangeli A,Hancox JC,Witchel HJ

更新日期：2003-07-17 00:00:00

abstract：:Fibroblast growth factors (FGFs) and their receptors are expressed in a variety of mammalian tissues, playing a role in development and cell proliferation. While analyzing human sperm motility, we found that sperm treated with endo-β-galactosidase (EBG), which specifically hydrolyzes poly-N-acetyllactosamine type glyc...

journal_title：FEBS letters

authors： Sugihara K,Shibata TK,Takata K,Kimura T,Kanayama N,Williams R,Hatakeyama S,Akama TO,Kuo CW,Khoo KH,Fukuda MN

更新日期：2013-10-01 00:00:00

abstract：:Several deletion mutants of Raf-1 were expressed with v-ras p21 or 14-3-3 protein in COS-7 cells and Sf9 cells and the interaction of Raf-1 with ras p21 or with 14-3-3 protein in intact cells was examined. Raf(1-135) (residues 1-135) and Raf(1-322) interacted with v-ras p21, but other deletion mutants such as Raf(136-...

journal_title：FEBS letters

authors： Koyama S,Williams LT,Kikuchi A

更新日期：1995-07-17 00:00:00

abstract：:A single copy of a glucocorticoid-responsive element (GRE) is sufficient in mediating the combinatorial response of a promoter to both glucocorticoids and insulin in HepG2 cells. This requires the presence of active glucocorticoid receptor (GR) since the response is significantly inhibited by the anti-glucocorticoid R...

journal_title：FEBS letters

authors： Georgakopoulos A,Tsawdaroglou N

更新日期：1996-03-04 00:00:00

abstract：:Development of targeted therapies for triple-negative breast cancer (TNBC, a more aggressive subtype) is an unmet medical need. We analyzed data from 887 patients with invasive breast cancer and observed that increased Wnt and histone deacetylase (HDAC) activities are associated with estrogen receptor 1 (ESR1) and pro...

journal_title：FEBS letters

authors： Sulaiman A,Sulaiman B,Khouri L,McGarry S,Nessim C,Arnaout A,Li X,Addison C,Dimitroulakos J,Wang L

更新日期：2016-12-01 00:00:00

abstract：:A method was developed to study amino acids as possible precursors of PQQ biosynthesis. Cultures of Hyphomicrobium X, growing on [13C]methanol, were supplemented with unlabelled amino acids. Uptake and participation in metabolism were determined via gas chromatography/mass spectrometry of derivatized amino acids, obta...

journal_title：FEBS letters

authors： van Kleef MA,Duine JA

更新日期：1988-09-12 00:00:00