Abstract：
:The effects of cold exposure and T4 administration on fructose 2,6-bisphosphate levels, phosphofructokinase-2 and pyruvate kinase activities were examined in rat brown adipose tissue. Cold adaptation (14 days) gave rise to a 2-fold increase in the amount of fructose 2,6-bisphosphate and phosphofructokinase-2 activity, and increased the pyruvate kinase activity 4-fold. If, in addition, the cold-acclimated rats were treated with T4, these parameters were again significantly enhanced. The effect on phosphofructokinase-2 was on the Vmax, without modification of the Km (for both fructose 6-phosphate and ATP) of the enzyme. In the hypothyroid state, however, the activity of pyruvate kinase remains unchanged. These data support previous observations on stimulation of glycolytic flux during cold adaptation in brown adipose tissue, and a permissive role of thyroid hormones in the process.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sobrino F,Gualberto A,Pintado Edoi
10.1016/0014-5793(88)80804-4subject
Has Abstractpub_date
1988-02-29 00:00:00pages
91-4issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(88)80804-4journal_volume
229pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract：:The addition of a poly-His C-terminal extension, designed to facilitate the purification of the protein, to the beta-lactamase of a thermophilic Bacillus licheniformis strain modified the site of action of the signal peptidase. This resulted in the secretion of a protein with a different N-terminus, showing that this ...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/s0014-5793(97)00908-3
更新日期：1997-08-18 00:00:00
abstract：:The addition of 1-acyl-sn-glycero-3-phosphocholine (1-acyl-GPC) to peroxisomes decreased the production of acid-soluble radioactivity formed by beta-oxidation of [1-(14)C]arachidonate due to substrate removal by esterification into the acceptor. This peroxisomal-associated acyl-CoA:1-acyl-GPC acyltransferase activity ...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(95)00565-q
更新日期：1995-06-26 00:00:00
abstract：:Rab proteins are small GTPases involved in the regulation of membrane traffic. Rab5a has been shown to regulate transport in the early endocytic pathway. Here we report the isolation of cDNA clones encoding two highly related isoforms, Rab5b and Rab5c. The two proteins share with Rab5a all the structural features requ...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(95)00477-q
更新日期：1995-06-05 00:00:00
abstract：:The effect on cytosolic Ca2+ concentration ([Ca2+]i) of cAMP analogues and the adenylate cyclase-stimulating agents forskolin, isoproterenol and glucagon has been examined in an insulin-secreting beta-cell line (HIT T-15) using fura 2. All these manipulations of the cAMP messenger system promoted a rise in [Ca2+]i whi...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(87)80884-0
更新日期：1987-08-10 00:00:00
abstract：:The effects of protein disulfide isomerase (PDI) on the four structured des species that accumulate in the rate-determining steps of ribonuclease A folding were investigated at pH 8.0 and 15 degrees C. The results indicate that PDI catalyzes the conversion of the kinetically trapped intermediates, des-[26-84] and des-...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/s0014-5793(02)02825-9
更新日期：2002-06-19 00:00:00
abstract：:Mature dermal glands of Xenopus laevis contain storage granules with a characteristic ellipsoid shape. A few major proteins are present in these granules, including two with the same amino-terminal sequence and apparent molecular masses of 26 and 28 kDa. Using antibodies against these proteins, positive clones were is...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(89)80644-1
更新日期：1989-06-05 00:00:00
abstract：:Several approaches have been used to identify the factors involved in mRNA splicing. None of them, however, comprises a straightforward reversible method for inhibiting the second step of splicing using an external reagent other than a chelator. This investigation demonstrates that the addition of boric acid to an in ...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/s0014-5793(03)00928-1
更新日期：2003-09-25 00:00:00
abstract：:We have recently developed an in vitro differentiation model of immortalized non-transformed human hepatocytes using butyrate, and observed the induction of inducible NO synthase (iNOS). In this study, we analyzed the effect of NO on the urea-synthetic capacity of these cells. The inhibition of iNOS during butyrate tr...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/s0014-5793(00)01599-4
更新日期：2000-06-02 00:00:00
abstract：:A cDNA was cloned and expressed that encodes human stress-activated protein kinase kinase-4 (SKK4), a novel MAP kinase kinase family member whose mRNA is widely expressed in human tissues. SKK4 activated SAPK1/JNK in vitro, but not SAPK2a/p38, SAPK2b/p38beta, SAPK3/ERK6 or SAPK4. It appears to be the mammalian homolog...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/s0014-5793(97)00990-3
更新日期：1997-09-01 00:00:00
abstract：:The functional molecular masses of the vacuolar membrane H+-ATPase in Saccharomyces cerevisiae under two kinetic conditions for ATP hydrolysis were measured by radiation inactivation. When vacuolar membrane vesicles were exposed to gamma-rays from 60Co, the activities catalyzing a single-cycle and multi-cycles of ATP ...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(89)80571-x
更新日期：1989-02-27 00:00:00
abstract：:The possible relationship between pyridoxal phosphate-dependent ornithine decarboxylase (ODC) activity and glucose-6-phosphate dehydrogenase (G6PD) activity has been studied in the osteoblasts of the growth-plate of metatarsals of rats fed a pyridoxine-deficient diet, which caused depressed G6PD levels. The G6PD activ...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(86)80579-8
更新日期：1986-05-26 00:00:00
abstract：:The fungal metabolite brefeldin A (BFA) is known to disrupt the Golgi apparatus resulting in redistribution of Golgi proteins to the endoplasmic reticulum and inhibition of protein secretion. BFA was found to inhibit protein synthesis in rat glioma C6 cells by up to 70% between 0.1 and 1 microgram/ml. Inhibition was b...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(92)81508-j
更新日期：1992-12-21 00:00:00
abstract：:The cholesterol content of rat liver plasma membranes was manipulated using either cholesterol-free or cholesterol-enriched liposomes. Removal of cholesterol from the membranes led to a marked increase in 5'-nucleotidase activity. However, increase in cholesterol content failed to exert any significant effect on 5'-nu...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(83)81118-1
更新日期：1983-06-27 00:00:00
abstract：:Under hypobaric hypoxia, antioxidant defenses of the heart are stressed by the enhanced production of ROS. Mammalian heart acclimatizes to hypoxia through altered gene expression, which we studied in murine heart exposed to 10h of acute hypobaric hypoxia (AHH), equivalent to 15000ft, using cDNA arrays. Functional clas...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/j.febslet.2007.08.044
更新日期：2007-10-02 00:00:00
abstract：:We investigated the role of p53 in nuclear factor (NF)-kappaB dependent, platelet-activating factor (PAF)-induced vascular endothelial growth factor (VEGF) expression. Transfected NF-kappaB subunits in ECV304 cells increased the tumor necrosis factor-alpha promoter activity, which was completely inhibited by p53. Tran...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/j.febslet.2006.04.042
更新日期：2006-05-29 00:00:00
abstract：:The mediator complex in the RNA polymerase II holoenzyme is known to be involved in transcriptional activation. The role of the essential mediator component Srb7p has been difficult to investigate, since no conditional lethal allele has been available to date. While the expression of Srb7p under the control of a repre...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/s0014-5793(00)02123-2
更新日期：2000-10-27 00:00:00
abstract：:The critical urea concentration (C3*) which destabilizes the structure of bovine serum albumin and chymotrypsinogen was determined by UV difference spectroscopy. The increase of the relative content of mobile rotating water molecules in aqueous urea was formerly shown by millimeter spectroscopy [1]. The rise of rotato...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(93)81152-p
更新日期：1993-01-04 00:00:00
abstract：:We synthesized and evaluated by surface plasmon resonance 64 LNA/2'-O-methyl sequences corresponding to all possible combinations of such residues in a kissing aptamer loop complementary to the 6-nt loop of the TAR element of HIV-1. Three combinations of LNA/2'-O-methyl nucleoside analogues where one or two LNA units ...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/j.febslet.2007.01.047
更新日期：2007-02-20 00:00:00
abstract：:Measurements of the effects of NH3/NH4+ on glucose-induced electrical activity in beta-cells from microdissected mouse islets of Langerhans and on intracellular pH in single collagenase-isolated islets pre-loaded with a fluorescent pH probe were performed and are reported here. Application of NH3/NH4+ (15 mM) in the p...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(86)80539-7
更新日期：1986-05-05 00:00:00
abstract：:Currently described substrates of the bacterial Tat protein transport system are directed for export by signal peptides containing a pair of invariant arginine residues. The signal peptide of the TtrB subunit of Salmonella enterica tetrathionate reductase contains a single arginine residue but is nevertheless able to ...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/s0014-5793(01)02428-0
更新日期：2001-05-18 00:00:00
abstract：:Mechanisms underlying the association between fibroblastic growth factor 23 (FGF-23) and inflammation are uncertain. We found that FGF-23 was markedly up-regulated in LPS/INF-γ-induced proinflammatory M1 macrophages and Hyp mouse-derived peritoneal macrophages, but not in IL-4-induced M2 anti-inflammatory macrophages....
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1002/1873-3468.12040
更新日期：2016-01-01 00:00:00
abstract：:Structural differences between platelet and endothelial cell thrombospondin (TBSP) were found in two protease-resistant domains (70 and 18 kDa). The 70 kDa fragment is involved in the binding of TBSP to fibrinogen and the 18 kDa fragment in the attachment to various cultured cells. Despite these structural differences...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(88)80001-2
更新日期：1988-02-15 00:00:00
abstract：:Escherichia coli phosphofructokinase-2 (Pfk-2) is a homodimer whose subunits consist of a large domain and an additional beta-sheet that provides the interfacial contacts between the subunits, creating a beta-barrel flattened-like structure with the adjacent subunit's beta-sheet. To determine how the structural organi...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/j.febslet.2009.05.034
更新日期：2009-06-18 00:00:00
abstract：:The crystal structure of the free form of IF1 from Mycobacterium tuberculosis has been determined at 1.47 A resolution. The structure adopts the expected OB fold and matches the high structural conservation among IF1 orthologues. In order to further explore the function of Mtb-IF1, we built a model of its interaction ...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/j.febslet.2010.01.051
更新日期：2010-03-05 00:00:00
abstract：:Carotenoids present in lipids extracted from the cyanobacterium Synechococcus 6716 indicate trans-membrane potential in proteoliposomes reconstituted from these lipids and the ATPase complex isolated from the same organism. A carotenoid absorbance band shift to a longer wavelength is obtained with valinomycin-induced ...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(85)80661-x
更新日期：1985-05-06 00:00:00
abstract：:Incubation of HER14 cells with phorbol myristate acetate (PMA) decreases epidermal growth factor (EGF)-dependent protein tyrosine phosphorylation, except for a 40-kDa MAP kinase II-like protein, whose tyrosine phosphorylation is further enhanced. The inhibitory effect of PMA on EGF-dependent protein tyrosine phosphory...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(94)80374-9
更新日期：1994-02-14 00:00:00
abstract：:The regulation by insulin and carbohydrates of the gene expression of three key enzymes involved in glucose metabolism was studied in the liver of the Wistar fatty rat, a model of obese non-insulin-dependent diabetes mellitus. A high glucose or fructose diet, or insulin administration caused a similar magnitude of inc...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(93)80982-z
更新日期：1993-08-09 00:00:00
abstract：:The phytocannabinoid Delta(9)-Tetrahydrocannabinol (Delta(9)-THC), the main psychoactive cannabinoid in cannabis, activates a number of signalling cascades including p53. This study examines the role of Delta(9)-THC in regulating the p53 post-translational modifier proteins, Murine double minute (Mdm2) and Small Ubqui...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/j.febslet.2009.09.056
更新日期：2009-11-03 00:00:00
abstract：:The HBx protein of hepatitis B virus is involved in deregulation of cell cycle and development of hepatocellular carcinoma. Since c-Myc also plays an important role in cell proliferation and tumor development, we studied its regulation by HBx in a human hepatoma cell line. Co-expression of HBx and c-Myc resulted in in...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/j.febslet.2005.12.034
更新日期：2006-01-23 00:00:00
abstract：:The mechanism of stimulation of hepatic respiration by fatty acids was studied in isolated rat hepatocytes. Stimulation of respiration by fatty acids varied from about 35% to about 105% depending on chain length. The stimulatory effect of octanoate (1 mM) or oleate (0.5 mM) was prevented by oligomycin (2 micrograms/ml...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(85)80161-7
更新日期：1985-12-02 00:00:00
