Unexpected influence of a C-terminal-fused His-tag on the processing of an enzyme and on the kinetic and folding parameters.

abstract：:The F-box protein Skp2 is important for S phase entry and binds to Skp1 and the cyclin A-Cdk2 complex. Here we report the cloning, analysis of genomic organization and characterization of a novel gene product related to Skp2 named FBL2. The human FBL2 gene was found to be a highly interrupted gene of at least 126.6 kb...

journal_title：FEBS letters

authors： Ilyin GP,Rialland M,Glaise D,Guguen-Guillouzo C

更新日期：1999-10-01 00:00:00

abstract：:Activator protein 1 (AP1) is a complex of Fos and Jun, and it regulates the transcription of genes possessing the AP1-binding sequence. The purpose of this study was to detect living cells that express AP1 after stimulation with a tumor promoter. The Fos and Jun components of AP1 were induced rapidly and transiently i...

journal_title：FEBS letters

authors： Shimokawa N,Miura M

更新日期：1996-06-10 00:00:00

abstract：:Macrophages contain arginase and an inducible NO synthase, demonstrated by using L-arginine, the common substrate, for production of both nitric oxide and urea. Arginase was inhibited by nitrite, the stable end product of NO. This inhibition was non-competitive, and could not be explained by the reaction of nitrite wi...

journal_title：FEBS letters

authors： Hrabák A,Bajor T,Temesi A,Mészáros G

更新日期：1996-07-22 00:00:00

abstract：:Nuclear ionic channels (NICs) represent ubiquitous structures of living cells, although little is known about their functional properties and encoding genes. To characterize NICs, liver nuclear membrane vesicles were reconstituted into either planar lipid bilayers or proteoliposomes. Reconstitution of nuclear envelope...

journal_title：FEBS letters

authors： Guihard G,Proteau S,Payet MD,Escande D,Rousseau E

更新日期：2000-07-07 00:00:00

abstract：:The nucleotide sequences of the dnaQ genes from Salmonella typhimurium and Buchnera aphidicola, encoding the epsilon-subunit of the DNA polymerase III holoenzyme, have been determined. The Salmonella typhimurium dnaQ protein consists of 243 amino acid residues with a calculated molecular weight of 27224. The Buchnera ...

journal_title：FEBS letters

authors： Huang Y,Braithwaite DK,Ito J

更新日期：1997-01-02 00:00:00

abstract：:Wilms tumor (WT) is a genetically heterogeneous childhood kidney tumor. Several genetic mutations have been identified in WT patients, including inactivation of WTX, somatic stabilizing CTNNB1, and p53 mutations. However, the molecular mechanisms in tumorigenesis remain largely unexplored. Stat3 is a transcription fac...

journal_title：FEBS letters

authors： Cao X,Liu D,Yan X,Zhang Y,Yuan L,Zhang T,Fu M,Zhou Y,Wang J

更新日期：2013-03-18 00:00:00

abstract：:In Alzheimer's disease (AD) brain, microtubule-associated protein tau is abnormally modified by hyperphosphorylation and glycosylation, and is aggregated as neurofibrillary tangles of paired helical filaments. To investigate the role of tau glycosylation in neurofibrillary pathology, we isolated various pools of tau p...

journal_title：FEBS letters

authors： Liu F,Zaidi T,Iqbal K,Grundke-Iqbal I,Merkle RK,Gong CX

更新日期：2002-02-13 00:00:00

abstract：:To gain insight into the nuclear organization of the mouse Ig kappa locus and how it may relate to the formation of synapses during recombination, we have studied the kinetics of rearrangement of different V kappa gene families to J kappa gene segments in the pre-B cell line, 103bcl2. Remarkably, V kappa gene families...

journal_title：FEBS letters

authors： Li S,Garrard WT

更新日期：2003-02-11 00:00:00

abstract：:During steady-state, the Pi released in the medium is derived from glucose-6-phosphate which continuously regenerates the ATP hydrolyzed. A membrane potential (delta psi) can be built up in submitochondrial particles using glucose-6-phosphate and hexokinase as an ATP-regenerating system. The energy derived from the me...

journal_title：FEBS letters

authors： de Meis L,Grieco MA,Galina A

更新日期：1992-08-17 00:00:00

abstract：:In rat brain astroglia-enriched cultures long-term treatment with interleukin-1beta induces NO release and stimulation of soluble guanylyl cyclase. The cGMP formed is recovered in the extracellular medium but not in the cell monolayer. The interleukin-1beta effect is mediated by type I receptor and potentiated by inte...

journal_title：FEBS letters

authors： Pedraza CE,Baltrons MA,García A

更新日期：2001-11-02 00:00:00

abstract：:Protein import into isolated tobacco mitochondria was investigated using mitochondria from leaves harvested at different times of the day and night. Efficient import was only detected with mitochondria isolated from leaves harvested during the dark period of the growth cycle, only low levels of import were detected fr...

journal_title：FEBS letters

authors： Dessi P,Whelan J

更新日期：1997-09-29 00:00:00

abstract：:The 5.8S and 28S rRNA sequences of the oomycete Phytophthora megasperma were determined in order to study the secondary structure of these molecules and to assess the phylogenetic position of the oomycetes among the eukaryotes. Preliminary results point to an affiliation between the oomycetes, dinoflagellates and cili...

journal_title：FEBS letters

authors： Van der Auwera G,Chapelle S,De Wachter R

更新日期：1994-01-31 00:00:00

abstract：:ISG20 is an interferon-induced antiviral exoribonuclease that acts on single-stranded RNA and also has minor activity towards single-stranded DNA. It belongs to the DEDDh group of RNases of the DEDD exonuclease superfamily. We have solved the crystal structure of human ISG20 complexed with two Mn2+ ions and uridine 5'...

journal_title：FEBS letters

authors： Horio T,Murai M,Inoue T,Hamasaki T,Tanaka T,Ohgi T

更新日期：2004-11-05 00:00:00

abstract：:Iron dependent regulator, IdeR, regulates the expression of genes in response to intracellular iron levels in M. tuberculosis. Orthologs of IdeR are present in all the sequenced genomes of mycobacteria. We have used a computational approach to identify conserved IdeR regulated genes across the mycobacteria and the gen...

journal_title：FEBS letters

authors： Yellaboina S,Ranjan S,Vindal V,Ranjan A

更新日期：2006-05-15 00:00:00

abstract：:Gene duplication provides genetic material required for functional diversification. An interesting example is the amyloid precursor protein (APP) protein family. The APP gene family has experienced both expansion and contraction during evolution. The three mammalian members have been studied quite extensively in combi...

journal_title：FEBS letters

authors： Shariati SA,De Strooper B

更新日期：2013-06-27 00:00:00

abstract：:The H3K9 protein lysine methyltransferase SUV39H2 was reported to methylate K134 of H2AX and stimulate H2AX phosphorylation during DNA damage response [Sone K et al. (2014) Nat Commun 5, 5691]. However, the sequence context of H2AX-K134 differs from the specificity of SUV39H2. We performed in vitro methylation reactio...

journal_title：FEBS letters

authors： Schuhmacher MK,Kudithipudi S,Jeltsch A

更新日期：2016-06-01 00:00:00

abstract：:The protein RPE65 has an important role in retinoid processing and/or retinoid transport in the eye. Retinoids are involved in cell differentiation, embryogenesis and carcinogenesis. Since the kidney is known as an important site for retinoid metabolism, the expression of RPE65 in normal kidney and transformed kidney ...

journal_title：FEBS letters

authors： Ma JX,Zhang D,Laser M,Brownlee NA,Re GG,Hazen-Martin DJ,Redmond TM,Crouch RK

更新日期：1999-06-11 00:00:00

abstract：:The rapid conversion of the carotenoid violaxanthin to zeaxanthin via antheraxanthin (xanthophyll cycle) in potato leaves exposed at 23 degrees C to a strong white light of 2000 microE.m-2.s-1 was associated with a slight inhibition of photosynthetic electron transport (as estimated from chlorophyll fluorescence measu...

journal_title：FEBS letters

authors： Sarry JE,Montillet JL,Sauvaire Y,Havaux M

更新日期：1994-10-17 00:00:00

abstract：:Heparin Binding Hemagglutinin A (HBHA) is hitherto the sole virulence factor associated with tuberculosis dissemination from the lungs, the site of primary infection, to epithelial cells. We have previously reported the solution structure of HBHA, a dimeric and elongated molecule. Since oligomerisation of HBHA is asso...

journal_title：FEBS letters

authors： Esposito C,Carullo P,Pedone E,Graziano G,Del Vecchio P,Berisio R

更新日期：2010-03-19 00:00:00

abstract：:Previously, we found that intracellular reactive oxygen species (ROS) affect photomorphogenesis in Neurospora crassa. In this study, we investigated the physiological roles of ROS in the response to light and found that the exposure of mycelia to air was important for the light-induced carotenogenesis. Mycelia treated...

journal_title：FEBS letters

authors： Iigusa H,Yoshida Y,Hasunuma K

更新日期：2005-07-18 00:00:00

abstract：:It was found for RNases of different specificities that changes in the free energy for substrate-enzyme binding induced by variations in the nucleotide base structure are accompanied by proportional changes in kcat/Km. This was considered to be a consequence of the strain in the enzyme-substrate complex. ...

journal_title：FEBS letters

authors： Yakovlev GI,Bocharov AL,Moiseyev GP

更新日期：1984-10-01 00:00:00

abstract：:The chemotherapeutic activity of three synthetic antibacterial peptides was investigated. KLKLLLLLKLK-NH2 and its D-enantiomer showed significant chemotherapeutic activity in MRSA-infected mice, whereas KLKLLLKLK-NH2, which showed the highest antibacterial activity among them in vitro, was found to have almost no abil...

journal_title：FEBS letters

authors： Nakajima Y,Alvarez-Bravo J,Cho J,Homma K,Kanegasaki S,Natori S

更新日期：1997-09-22 00:00:00

abstract：:SSTR3, a somatostatin (SST) receptor, is an adenylyl cyclase (AC)-inhibiting receptor. To assign the G-protein alpha-subunit (G alpha) linked to this receptor, we created a novel reporter system which utilizes the well-established facts that the C-terminal 5 residues of G alpha are the receptor contact site and G alph...

journal_title：FEBS letters

authors： Komatsuzaki K,Murayama Y,Giambarella U,Ogata E,Seino S,Nishimoto I

更新日期：1997-04-07 00:00:00

abstract：:We report the identification, gene expression and biological activity of two parathyroid hormones (PTH; PTHA and PTHB), two PTH-related peptides (PTHrP; PTHrPA and PTHrPB) and a PTH-like ligand (PTH-L) with hybrid characteristics in puffer fishes (Takifugu rubripes and Tetraodon fluviatilis). Experimental data are con...

journal_title：FEBS letters

authors： Canario AV,Rotllant J,Fuentes J,Guerreiro PM,Rita Teodósio H,Power DM,Clark MS

更新日期：2006-01-09 00:00:00

abstract：:Internalization of membrane proteins has been studied for more than three decades without solving all the underlying mechanisms. Our knowledge of the clathrin-coated endocytosis is sufficient to understand the basic principles. However, more detailed insight is required to recognize why different proteins enter clathr...

journal_title：FEBS letters

authors： Strous GJ,Gent J

更新日期：2002-10-02 00:00:00

abstract：:The dependence of CMP-N-acetylneuraminate:GM1 sialyltransferase (SAT IV) activity of rat liver Golgi apparatus on GM1 ganglioside ceramide composition was evaluated. SAT IV activity was assayed on GM1 molecular species carrying homogeneous ceramide moieties containing long chain bases of different length (18 or 20 C a...

journal_title：FEBS letters

authors： Pitto M,Palestini P,Masserini M

更新日期：1996-04-01 00:00:00

abstract：:Mona is an SH3 and SH2 domain-containing adapter molecule that is induced during monocytic differentiation. Here we have first shown that M-CSFR is the major Mona partner in M-CSF signaling, the interaction being mediated through tyrosine 697 of the receptor. Next we asked whether Mona expression would alter the Ras/M...

journal_title：FEBS letters

authors： Bourgin C,Bourette R,Mouchiroud G,Arnaud S

更新日期：2000-09-01 00:00:00

abstract：:Although signal transducer and activator of transcription 1 (STAT1) mediated regulation of p53 transcription and apoptosis has been previously reported, modulation of other members of the p53 family of transcription factors remains poorly understood. In this study, we found that STAT1 and TA-p73 can interact directly ...

journal_title：FEBS letters

authors： Soond SM,Carroll C,Townsend PA,Sayan E,Melino G,Behrmann I,Knight RA,Latchman DS,Stephanou A

更新日期：2007-03-20 00:00:00

abstract：:The primary structure of a new sarafotoxin, SRTX-d, from the venom of Atractaspis engaddensis is described. SRTX-d differs from SRTX-b in two substitutions: Ile19 instead of Val and Thr2 instead of Ser. The toxicity of SRTX-d and its vasoconstriction potency are very low in comparison to SRTX-a and SRTX-b, whereas its...

journal_title：FEBS letters

authors： Bdolah A,Wollberg Z,Fleminger G,Kochva E

更新日期：1989-10-09 00:00:00

abstract：:Whether all inflammatory agents activate nuclear transcription factors NF-kappaB and activated protein-1 (AP-1) through the same mechanism is not known. We examined the effect of the phosphatidylinositol-3-kinase (PI-3K) inhibitor wortmannin on the activation of NF-kappaB and AP-1 by different inflammatory agents. Wor...

journal_title：FEBS letters

authors： Manna SK,Aggarwal BB

更新日期：2000-05-04 00:00:00