Abstract:
:The 5.8S and 28S rRNA sequences of the oomycete Phytophthora megasperma were determined in order to study the secondary structure of these molecules and to assess the phylogenetic position of the oomycetes among the eukaryotes. Preliminary results point to an affiliation between the oomycetes, dinoflagellates and ciliates, a cluster which seems related to the fungi. In the course of this work, we developed a set of primers which allow sequencing and PCR amplification of eukaryotic large ribosomal subunit RNA genes of a wide range of phylogenetically distant organisms.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Van der Auwera G,Chapelle S,De Wachter Rdoi
10.1016/0014-5793(94)80350-1subject
Has Abstractpub_date
1994-01-31 00:00:00pages
133-6issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(94)80350-1journal_volume
338pub_type
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