Abstract:
:The H3K9 protein lysine methyltransferase SUV39H2 was reported to methylate K134 of H2AX and stimulate H2AX phosphorylation during DNA damage response [Sone K et al. (2014) Nat Commun 5, 5691]. However, the sequence context of H2AX-K134 differs from the specificity of SUV39H2. We performed in vitro methylation reactions with SUV39H2 (and its homolog SUV39H1) using H2AX protein and peptides, but no methylation at K134 or any other lysine in H2AX was detected. Positive controls demonstrated the functionality of the assays. While our data cannot finally exclude H2AX methylation of SUV39H2 in cells, additional experimental evidence is required to validate this claim.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Schuhmacher MK,Kudithipudi S,Jeltsch Adoi
10.1002/1873-3468.12216subject
Has Abstractpub_date
2016-06-01 00:00:00pages
1713-9issue
12eissn
0014-5793issn
1873-3468journal_volume
590pub_type
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